##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938480_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2455490 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.786366875857773 31.0 31.0 34.0 30.0 34.0 2 32.03995943783114 31.0 31.0 34.0 30.0 34.0 3 32.10093993459554 33.0 31.0 34.0 30.0 34.0 4 35.760310976627885 37.0 35.0 37.0 35.0 37.0 5 35.646661562458 37.0 35.0 37.0 33.0 37.0 6 35.676748021779765 37.0 35.0 37.0 33.0 37.0 7 35.59505271860199 37.0 35.0 37.0 33.0 37.0 8 35.563198384029256 37.0 35.0 37.0 33.0 37.0 9 37.10611853438621 39.0 37.0 39.0 33.0 39.0 10-11 37.02761546575225 39.0 37.0 39.0 33.0 39.0 12-13 36.908264134653365 39.0 37.0 39.0 33.0 39.0 14-15 37.9249310728205 40.0 37.0 41.0 33.0 41.0 16-17 38.0128943713882 40.0 37.0 41.0 33.0 41.0 18-19 38.13467291660727 40.0 37.0 41.0 33.0 41.0 20-21 38.08026890763147 40.0 37.0 41.0 33.0 41.0 22-23 37.9513233611214 40.0 37.0 41.0 33.0 41.0 24-25 37.826862255598684 40.0 37.0 41.0 32.0 41.0 26-27 37.609674647422715 40.0 37.0 41.0 32.0 41.0 28-29 37.46297521065042 39.0 36.5 41.0 32.0 41.0 30-31 37.20101323971997 39.0 36.0 41.0 31.0 41.0 32-33 37.108017340734435 39.0 36.0 41.0 31.0 41.0 34-35 36.978073622779974 39.0 36.0 41.0 30.5 41.0 36-37 36.8572482478039 39.0 35.5 41.0 30.0 41.0 38-39 36.63586310675262 39.0 35.0 40.0 30.0 41.0 40-41 36.40353839763143 39.0 35.0 40.0 30.0 41.0 42-43 36.14260921445414 38.0 35.0 40.0 29.5 41.0 44-45 35.859619057703355 38.0 35.0 40.0 29.0 41.0 46-47 35.45871964455159 38.0 34.0 40.0 27.5 41.0 48-49 35.04969272935341 38.0 34.0 40.0 26.0 41.0 50-51 34.642847456108555 38.0 33.0 40.0 25.0 41.0 52-53 34.02065921669402 37.5 33.0 40.0 23.5 41.0 54-55 33.568124895642015 37.0 32.0 40.0 23.0 41.0 56-57 33.15443658903111 36.5 31.5 39.0 22.0 40.5 58-59 32.5962681582902 36.0 31.0 39.0 19.5 40.0 60-61 32.076040627328965 35.5 31.0 39.0 18.0 40.0 62-63 31.523200257382435 35.0 30.0 38.5 13.5 40.0 64-65 30.834707125665346 34.5 29.0 38.0 8.0 40.0 66-67 30.153451653234182 34.0 28.0 37.5 7.5 40.0 68-69 29.402372846152907 34.0 27.5 37.0 3.5 39.0 70-71 28.605299756871336 33.0 26.0 36.0 2.0 39.0 72-73 27.991013606245595 33.0 26.0 36.0 2.0 38.5 74-75 27.253291196461806 32.0 24.5 35.0 2.0 38.0 76-77 26.131835804666277 30.5 23.0 34.0 2.0 36.0 78-79 26.601314605231543 32.0 24.0 35.0 2.0 36.0 80-81 26.205909614781568 32.0 23.0 35.0 2.0 36.0 82-83 25.507259854448602 31.0 20.5 34.0 2.0 36.0 84-85 24.70739404355139 31.0 18.5 34.0 2.0 35.0 86-87 23.780820935943538 30.0 10.0 34.0 2.0 35.0 88-89 22.790297863155622 29.5 4.5 34.0 2.0 35.0 90-91 21.920178050002242 29.0 2.0 34.0 2.0 35.0 92-93 20.83068409971126 28.0 2.0 33.5 2.0 35.0 94-95 19.652789463610116 26.5 2.0 33.0 2.0 34.0 96-97 18.273376393306428 24.5 2.0 32.0 2.0 34.0 98-99 16.516424420380453 11.0 2.0 32.0 2.0 34.0 100-101 12.380746205441683 2.0 2.0 27.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27714451400047224 1101 2 0.28048290661465103 1101 3 0.28921400003753206 1101 4 0.1308419960366649 1101 5 0.11923809945286479 1101 6 0.2261072936722286 1101 7 0.2619573008559186 1101 8 0.25751086529812994 1101 9 0.29514799222869925 1101 10-11 0.37342511992392957 1101 12-13 0.3391122590128006 1101 14-15 0.45277152370390894 1101 16-17 0.44405253594567284 1101 18-19 0.4573721151276473 1101 20-21 0.41667131231558585 1101 22-23 0.31354217459860223 1101 24-25 0.319873881121822 1101 26-27 0.480242432568609 1101 28-29 0.49802991919826667 1101 30-31 0.4824682005637939 1101 32-33 0.4177666104792124 1101 34-35 0.2791238032043921 1101 36-37 0.3522883779114352 1101 38-39 0.46685696837752744 1101 40-41 0.5865464927741115 1101 42-43 0.6272120940384553 1101 44-45 0.5429702986378899 1101 46-47 0.6896051651056112 1101 48-49 0.7576221152068925 1101 50-51 0.9115202084839424 1101 52-53 0.8905532179541993 1101 54-55 0.8209827327992869 1101 56-57 1.029367765115559 1101 58-59 0.994887184486501 1101 60-61 1.120603913264219 1101 62-63 1.2476492260820145 1101 64-65 1.1346286422359064 1101 66-67 1.2051804664250483 1101 68-69 1.4896353863197618 1101 70-71 1.4432574432274095 1101 72-73 1.5489276379644146 1101 74-75 1.467602242871994 1101 76-77 1.5416369653302056 1101 78-79 1.8240243950830681 1101 80-81 1.8312650003612987 1101 82-83 2.0710036471711746 1101 84-85 2.2973696106530284 1101 86-87 2.347967855630973 1101 88-89 2.3102600787304617 1101 90-91 2.5137247021758995 1101 92-93 2.771048462960323 1101 94-95 2.5024080983534454 1101 96-97 2.7310549931743218 1101 98-99 2.5201044137623363 1101 100-101 2.2078152734094605 1102 1 0.18385102822340116 1102 2 0.22716138230353522 1102 3 0.1764555198018627 1102 4 0.15279067415239922 1102 5 0.18887691387755723 1102 6 0.16773599149071572 1102 7 0.19821889280052574 1102 8 0.15147216075418157 1102 9 0.315484454637172 1102 10-11 0.2258571785775132 1102 12-13 0.3201005226129112 1102 14-15 0.4296690978023676 1102 16-17 0.3803269157351892 1102 18-19 0.2949177014266553 1102 20-21 0.3363612781199379 1102 22-23 0.38937930481675664 1102 24-25 0.3754526968726566 1102 26-27 0.4800269337005787 1102 28-29 0.38153170476031306 1102 30-31 0.47456862777253406 1102 32-33 0.2709853584071027 1102 34-35 0.5108414475886249 1102 36-37 0.5062630295610688 1102 38-39 0.438667348318603 1102 40-41 0.5885598679126431 1102 42-43 0.583128129995977 1102 44-45 0.507403986557641 1102 46-47 0.46108732369383176 1102 48-49 0.549842842882633 1102 50-51 0.7717097527630372 1102 52-53 0.8119572286012655 1102 54-55 0.6697318921168858 1102 56-57 0.8614382592947223 1102 58-59 1.1582594812781792 1102 60-61 1.187607010764438 1102 62-63 1.3900470890910448 1102 64-65 1.4373415598537669 1102 66-67 1.6248514872739719 1102 68-69 1.691898834869999 1102 70-71 1.8144492703736361 1102 72-73 1.7532783469793713 1102 74-75 1.5901206905223475 1102 76-77 1.6904045959793557 1102 78-79 1.917845497868921 1102 80-81 2.087680709518345 1102 82-83 2.139135390391253 1102 84-85 2.305703496547565 1102 86-87 2.194656751045347 1102 88-89 2.057016228736618 1102 90-91 2.2411077407573003 1102 92-93 2.4337122184188367 1102 94-95 2.4293047252041298 1102 96-97 2.443889253231724 1102 98-99 2.4183235879517255 1102 100-101 2.242920275824943 1103 1 0.18532098708471523 1103 2 0.18492535859724057 1103 3 0.17162664995645116 1103 4 0.08739913264759736 1103 5 0.07659185183044315 1103 6 0.030697335421677963 1103 7 0.14775898079643923 1103 8 0.20396123916145825 1103 9 0.20286287831707028 1103 10-11 0.2563825605697829 1103 12-13 0.28963195947936526 1103 14-15 0.4430451735803942 1103 16-17 0.42298953909855896 1103 18-19 0.3357069520317353 1103 20-21 0.3192533030432543 1103 22-23 0.31542791060110176 1103 24-25 0.34299474053703705 1103 26-27 0.39397999582466525 1103 28-29 0.41709242899914756 1103 30-31 0.4440635905974233 1103 32-33 0.47661830674685035 1103 34-35 0.4479142866162178 1103 36-37 0.4731781412696989 1103 38-39 0.5764546386730558 1103 40-41 0.6683897229237488 1103 42-43 0.6558913685609582 1103 44-45 0.5519694001851789 1103 46-47 0.6004771807943996 1103 48-49 0.6111299549908225 1103 50-51 0.5921635338289803 1103 52-53 0.6403881941461194 1103 54-55 0.8104558926777443 1103 56-57 0.88013615433929 1103 58-59 0.8946515020775436 1103 60-61 0.9255763047534629 1103 62-63 1.0823058861624908 1103 64-65 1.2841287741442606 1103 66-67 1.2975001716882844 1103 68-69 1.3047523604343603 1103 70-71 1.4210487808150205 1103 72-73 1.3506086854089894 1103 74-75 1.3607633942935635 1103 76-77 1.3927994192314657 1103 78-79 1.504191651186364 1103 80-81 1.6764823539660867 1103 82-83 1.954438725200042 1103 84-85 2.1290451419300673 1103 86-87 1.926085827821371 1103 88-89 2.049071944209768 1103 90-91 2.16594491348755 1103 92-93 2.446217499365183 1103 94-95 2.3056540585447927 1103 96-97 2.1222643621727215 1103 98-99 2.236818401398004 1103 100-101 2.125013170993233 1104 1 0.22679461940323264 1104 2 0.2822759058859603 1104 3 0.26723171904743737 1104 4 0.1456035234103581 1104 5 0.2043246289984353 1104 6 0.1756692827259414 1104 7 0.18807324920590673 1104 8 0.12627459469406688 1104 9 0.23601873068625423 1104 10-11 0.21249860399472453 1104 12-13 0.24732135183991488 1104 14-15 0.3604738145516819 1104 16-17 0.37465080517746685 1104 18-19 0.45481062427703733 1104 20-21 0.384262846054213 1104 22-23 0.371038269643698 1104 24-25 0.2136724049513532 1104 26-27 0.4616625596192776 1104 28-29 0.385337796303439 1104 30-31 0.46125902086139803 1104 32-33 0.5252310581031736 1104 34-35 0.6364627249226729 1104 36-37 0.5546351627284807 1104 38-39 0.6121937164409985 1104 40-41 0.605476348968935 1104 42-43 0.5917095383097433 1104 44-45 0.446553992326308 1104 46-47 0.5819067747803288 1104 48-49 0.6390241580169018 1104 50-51 0.6148084375935454 1104 52-53 0.6398067111380357 1104 54-55 0.81998012122218 1104 56-57 1.1549352468141265 1104 58-59 1.1312291503852947 1104 60-61 0.9925078292115117 1104 62-63 1.0714354987249912 1104 64-65 1.4239862439109991 1104 66-67 1.4138721602624003 1104 68-69 1.676834846629287 1104 70-71 1.7294344347544133 1104 72-73 1.770024145755709 1104 74-75 1.714782342406238 1104 76-77 1.8104642555131214 1104 78-79 1.7949967066922632 1104 80-81 1.9705967167507623 1104 82-83 2.0689615463497617 1104 84-85 2.499731554318849 1104 86-87 2.1340254379807533 1104 88-89 2.173481520257198 1104 90-91 2.4746097307294974 1104 92-93 2.509392283380265 1104 94-95 2.17644879144083 1104 96-97 2.405526605348914 1104 98-99 2.374871472786104 1104 100-101 2.3106477619250807 1105 1 0.16624489496479455 1105 2 0.15436560860582915 1105 3 0.12818240158924965 1105 4 0.11893320460592349 1105 5 0.09655888255392853 1105 6 0.16116854744182518 1105 7 0.1504232055939454 1105 8 0.15524109798584362 1105 9 0.34817481295259967 1105 10-11 0.3134991537580234 1105 12-13 0.27270589474961326 1105 14-15 0.3367099077744413 1105 16-17 0.303462772533571 1105 18-19 0.23620735328258746 1105 20-21 0.3113419905992316 1105 22-23 0.4108414718691762 1105 24-25 0.2785075633822629 1105 26-27 0.32483876361059316 1105 28-29 0.43018867058248844 1105 30-31 0.504651711146515 1105 32-33 0.5984654049047293 1105 34-35 0.47507805962749927 1105 36-37 0.4260981255835219 1105 38-39 0.5376437258627362 1105 40-41 0.5586544373079363 1105 42-43 0.470001804519498 1105 44-45 0.6674063391162193 1105 46-47 0.7137431201560034 1105 48-49 0.7496873318411801 1105 50-51 0.8387170214771231 1105 52-53 0.8129695207198395 1105 54-55 0.7961147122118888 1105 56-57 0.939471503023249 1105 58-59 0.9677857866887152 1105 60-61 0.9377194492260088 1105 62-63 1.014063425534598 1105 64-65 1.353108545845668 1105 66-67 1.3616927276258863 1105 68-69 1.4121728733481724 1105 70-71 1.5266487569623237 1105 72-73 1.5627417733425588 1105 74-75 1.5645000302145071 1105 76-77 1.5256281063427188 1105 78-79 1.4527236491730342 1105 80-81 1.4745220363613853 1105 82-83 1.7412992253385084 1105 84-85 1.716845802124162 1105 86-87 1.6760833010527811 1105 88-89 1.8881839973002101 1105 90-91 1.9716647090012245 1105 92-93 1.8772528882752937 1105 94-95 1.766623569544322 1105 96-97 1.8568414720750255 1105 98-99 1.7653104966551574 1105 100-101 1.4771643606969533 1106 1 0.09229717982714902 1106 2 0.10874463918623434 1106 3 0.05105876245188057 1106 4 0.1555326082533881 1106 5 0.06085054055267847 1106 6 -0.005012715993444772 1106 7 0.07157291781917507 1106 8 0.031144294712838416 1106 9 0.011356977851015415 1106 10-11 0.04662445713503871 1106 12-13 0.11138386437724535 1106 14-15 0.11877252479129652 1106 16-17 0.07322215121742914 1106 18-19 0.10052674860884281 1106 20-21 0.1234562683885656 1106 22-23 0.20795852339126242 1106 24-25 0.10317298186919288 1106 26-27 -0.15293843630619364 1106 28-29 0.1569413229644212 1106 30-31 0.21830367702960274 1106 32-33 0.16409365537280252 1106 34-35 0.11064424782527027 1106 36-37 0.10528646203729863 1106 38-39 0.24447230917873952 1106 40-41 0.2457375106038242 1106 42-43 0.30337154309518155 1106 44-45 0.31404318815581433 1106 46-47 -0.25060747966370656 1106 48-49 -0.0330496489507226 1106 50-51 0.3057061345760772 1106 52-53 0.17588124667505411 1106 54-55 0.07765032000607874 1106 56-57 0.15990524548216456 1106 58-59 0.32953947922044335 1106 60-61 0.3613447179313596 1106 62-63 0.4265556244112325 1106 64-65 0.6364280105152424 1106 66-67 0.557736691289989 1106 68-69 0.714724707478954 1106 70-71 0.5318165677937472 1106 72-73 0.7809253251073436 1106 74-75 0.7085035667237669 1106 76-77 0.82979910696681 1106 78-79 0.9541122491314304 1106 80-81 0.9783445868086496 1106 82-83 1.2388687420155762 1106 84-85 1.4686556627440908 1106 86-87 1.4170929397740935 1106 88-89 1.5121837199269343 1106 90-91 1.6901620392834502 1106 92-93 1.5621707164425516 1106 94-95 1.2946798763191651 1106 96-97 1.1873109263431054 1106 98-99 1.5117341149895296 1106 100-101 1.4104526208730856 1107 1 0.03180397779267352 1107 2 0.013829320902388531 1107 3 -0.013659078523339474 1107 4 0.0063627989289472 1107 5 0.01033247436414797 1107 6 0.046951098727042506 1107 7 -0.040860670705527014 1107 8 -0.04730097769702013 1107 9 0.10750114459955284 1107 10-11 0.02098830510849581 1107 12-13 0.05448084969303579 1107 14-15 0.1810288900903103 1107 16-17 0.10189943868181928 1107 18-19 0.0508415182186539 1107 20-21 0.16046342533503832 1107 22-23 0.17419201971454612 1107 24-25 0.21574336102864322 1107 26-27 0.2227169873291075 1107 28-29 0.26006370145882585 1107 30-31 0.114520017842338 1107 32-33 0.11789394104606288 1107 34-35 0.18799519515709306 1107 36-37 0.15499738790585127 1107 38-39 0.1903738803816637 1107 40-41 0.25195808361299044 1107 42-43 0.11779464181872612 1107 44-45 0.1035537648681526 1107 46-47 0.466891896843066 1107 48-49 0.4580007670893025 1107 50-51 0.3111248575107979 1107 52-53 0.24636658891614616 1107 54-55 0.3750735925519777 1107 56-57 0.5452966102899381 1107 58-59 0.6359648603070553 1107 60-61 0.42025197992699503 1107 62-63 0.5852221802346627 1107 64-65 0.6236615143960158 1107 66-67 0.8542194337532791 1107 68-69 1.0554252115637794 1107 70-71 0.8236673224094915 1107 72-73 1.1824961501589222 1107 74-75 1.0874653938234253 1107 76-77 1.081357840028577 1107 78-79 1.3267964441449962 1107 80-81 1.3698862399348073 1107 82-83 1.2615502919063708 1107 84-85 1.3469074633169527 1107 86-87 1.6319275440259382 1107 88-89 1.8648477364324982 1107 90-91 2.0292331477410386 1107 92-93 2.0306529449962767 1107 94-95 1.798939279730618 1107 96-97 1.9152351095669822 1107 98-99 2.0127351050211963 1107 100-101 1.7119716770932794 1108 1 0.04231876949068436 1108 2 0.05806871664330515 1108 3 0.03321905739989717 1108 4 -0.011707568449708106 1108 5 -0.051153312371887694 1108 6 0.04191989104146643 1108 7 0.0543742596997987 1108 8 -0.005883638990283657 1108 9 0.015193294986559636 1108 10-11 -0.03465398309480605 1108 12-13 -0.0780288967746472 1108 14-15 0.060771063337149656 1108 16-17 0.05249109199532853 1108 18-19 -0.011447210935450869 1108 20-21 -0.00483615530028203 1108 22-23 0.08312210661111408 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-0.4126264272347484 2316 52-53 -0.27374219099186803 2316 54-55 -0.14653008569606385 2316 56-57 -0.23856239211590946 2316 58-59 -0.2589077130311068 2316 60-61 -0.15014532942824843 2316 62-63 -0.26401429333259685 2316 64-65 -0.1526154796152035 2316 66-67 0.034529739278688965 2316 68-69 0.21590543365968173 2316 70-71 0.022445091574446963 2316 72-73 0.21587247581536673 2316 74-75 0.4628469417193166 2316 76-77 0.4522628750827522 2316 78-79 0.42738670936241263 2316 80-81 0.11222822340967298 2316 82-83 0.24016860907631354 2316 84-85 0.4472292956655508 2316 86-87 0.5884107002222869 2316 88-89 0.5281172918387682 2316 90-91 0.7218101894098581 2316 92-93 0.8962497559563758 2316 94-95 1.0666041721565058 2316 96-97 1.0146643667400248 2316 98-99 0.6998667634482665 2316 100-101 0.45855274669060186 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 13.0 4 49.0 5 87.0 6 189.0 7 459.0 8 1191.0 9 2157.0 10 3569.0 11 5537.0 12 7772.0 13 10984.0 14 14802.0 15 19918.0 16 24759.0 17 30292.0 18 31059.0 19 30969.0 20 32835.0 21 35242.0 22 39217.0 23 43087.0 24 48361.0 25 54543.0 26 62207.0 27 72273.0 28 85053.0 29 98658.0 30 113903.0 31 131717.0 32 153025.0 33 181176.0 34 216342.0 35 260595.0 36 296321.0 37 259834.0 38 85400.0 39 1887.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.19360126309512 19.102114038653774 15.084348912103929 23.619935786147177 2 16.188906348387874 20.22649016009247 39.33307429193314 24.25152919958651 3 19.633778966603774 23.414378667596296 29.553683896724724 27.398158469075206 4 13.761041584368089 16.84107856273086 35.68082134319423 33.717058509706824 5 14.193006348239287 36.72131441296298 34.2017296793626 14.883949559435125 6 33.47657697648942 35.590493139862104 16.739510240318634 14.193419643329843 7 29.508285515314665 31.616459443939902 20.21103730823583 18.6642177325096 8 26.324787577866392 35.72556656762322 19.17924257825736 18.77040327625303 9 26.085996825068435 16.942104332910063 19.61017085823336 37.36172798378814 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26.43720130430285 27.885908527739247 22.570397897084543 36-37 23.215040591139264 25.991076221094033 28.004139343227465 22.789743844539235 38-39 22.662299339239436 26.33148976397452 28.00510019342612 23.001110703359927 40-41 23.135476847050075 26.151008540832937 27.976517647911752 22.736996964205236 42-43 22.8204385198423 26.204526521117867 27.824178276746895 23.15085668229294 44-45 22.810834651646616 26.446630354593527 27.976070603083276 22.766464390676585 46-47 23.18384842113837 26.685739593461584 27.291097999888002 22.839313985512042 48-49 22.976289864752044 26.51930979152837 27.449002032180946 23.05539831153863 50-51 23.167738414735958 26.810942011574063 27.279158131370927 22.74216144231905 52-53 23.312923286187278 26.602959083523043 27.188117239329014 22.896000390960662 54-55 22.889382567226907 26.73617078464991 27.36516947737519 23.009277170747996 56-57 23.003066597705548 26.83761693185474 27.265311607866455 22.894004862573254 58-59 22.939779840276277 26.46750750359399 27.311147673173174 23.28156498295656 60-61 22.780076481679828 26.756105705989437 27.400559562449857 23.06325824988088 62-63 22.745012197158204 26.78426709129339 27.542343890628757 22.928376820919656 64-65 22.591227774025413 26.664022250562365 27.413669418529146 23.33108055688308 66-67 22.55430112475229 26.738829244373996 27.299494924401564 23.40737470647215 68-69 22.845555291066113 26.473972643465217 27.462645051476002 23.217827013992668 70-71 22.764682405548385 26.539448338213557 27.175838630986078 23.520030625251987 72-73 22.794412754626325 26.58315432935768 27.116185373523894 23.506247542492105 74-75 22.7348920174792 26.930083201316236 26.871866714993747 23.463158066210816 76-77 22.81935581085649 26.641362823713393 27.190133130250988 23.34914823517913 78-79 22.867940818329537 26.67540898150675 27.210434577212695 23.24621562295102 80-81 22.912287017722765 26.882641562607155 26.977918017044285 23.227153402625795 82-83 22.863016649840212 26.871065654120063 26.906456185992113 23.359461510047613 84-85 22.83711079886778 26.843586454069683 26.962876982914857 23.35642576414768 86-87 22.96345937915033 26.82842594029349 26.801790519337764 23.40632416121842 88-89 22.81683085657038 26.96514748583786 26.565919633148578 23.652102024443185 90-91 22.76628329552973 26.809854065654264 26.82044274102523 23.603419897790776 92-93 22.755628643896337 26.572098444456387 26.913295018969848 23.75897789267743 94-95 22.608531335306328 26.504720648310787 26.96623768218876 23.920510334194127 96-97 22.718891691452967 26.338821648392308 26.90681430321734 24.035472356937383 98-99 22.57991276690192 26.511633115997213 26.60855878052853 24.299895336572334 100-101 22.892333418041545 26.18601679177418 26.761645750254125 24.16000403993015 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 293.0 1 213.0 2 200.0 3 273.0 4 241.5 5 223.0 6 362.5 7 809.5 8 1325.0 9 1541.0 10 1401.0 11 1202.0 12 1260.5 13 1455.0 14 1599.0 15 1748.5 16 1889.5 17 2030.5 18 2326.5 19 2646.5 20 3010.0 21 3551.5 22 4275.0 23 5188.5 24 6481.0 25 8384.5 26 10808.5 27 13802.5 28 16813.0 29 19793.5 30 23430.0 31 28811.5 32 36007.0 33 39973.5 34 41903.5 35 45349.0 36 50446.5 37 56532.5 38 61054.0 39 64290.0 40 67847.5 41 73175.5 42 77860.0 43 81720.0 44 85360.5 45 89416.5 46 92257.0 47 94343.0 48 95929.5 49 95607.0 50 95222.0 51 93412.0 52 91413.0 53 91465.0 54 91909.0 55 86519.5 56 77773.0 57 70817.0 58 64668.0 59 56707.5 60 46580.0 61 38834.5 62 32885.0 63 25517.5 64 18655.5 65 13927.0 66 10417.5 67 8057.5 68 6196.5 69 4672.0 70 3602.0 71 2653.5 72 1985.0 73 1538.0 74 1143.0 75 907.5 76 679.5 77 404.5 78 242.0 79 156.5 80 94.5 81 52.5 82 32.0 83 15.5 84 8.0 85 4.5 86 4.0 87 1.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023131839266297156 2 0.011565919633148578 3 0.00134392728131656 4 0.0 5 2.443504147848291E-4 6 0.0 7 0.0 8 8.14501382616097E-5 9 1.629002765232194E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001486465023274377 26-27 0.0083893642409458 28-29 2.2398788021942668E-4 30-31 0.0 32-33 3.258005530464388E-4 34-35 0.009448216038346726 36-37 0.0011403019356625358 38-39 0.011769544978802601 40-41 0.01158628216771398 42-43 0.004744470553738765 44-45 6.7196364065828E-4 46-47 1.0181267282701212E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 6.108760369620727E-5 66-67 6.516011060928776E-4 68-69 0.0010384892628355236 70-71 0.0 72-73 1.8326281108862184E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.629002765232194E-4 82-83 0.0012624771430549504 84-85 0.005701509678312679 86-87 0.0046019328117809484 88-89 0.0 90-91 0.001486465023274377 92-93 5.701509678312679E-4 94-95 4.072506913080485E-5 96-97 2.0362534565402424E-5 98-99 0.0 100-101 8.14501382616097E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2455490.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.2594260742371 #Duplication Level Percentage of deduplicated Percentage of total 1 79.14220086836903 46.10779200843169 2 12.307186123847618 14.340192003283539 3 3.6091702771790772 6.308045668579449 4 1.5723767605771612 3.664230705747742 5 0.8506931278463831 2.478044669681394 6 0.5408432076673135 1.890552892490827 7 0.3356682023698932 1.3689085775008596 8 0.2522176129069525 1.1755242699018518 9 0.19522656900532426 1.0236409078223372 >10 1.0763552277226653 11.628168657663792 >50 0.07586679697267873 3.023543843467073 >100 0.03764573829785232 3.9978344972205697 >500 0.002729692342871753 1.095678053763951 >1k 0.001749802783890248 1.6808688148182274 >5k 6.999211135560993E-5 0.21697442962683552 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5321 0.2166980928450126 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4815 0.19609120786482537 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3071 0.1250666873007017 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.072506913080485E-5 0.0 3 0.0 0.0 0.0 4.072506913080485E-5 0.0 4 0.0 0.0 0.0 4.072506913080485E-5 0.0 5 0.0 0.0 0.0 4.072506913080485E-5 0.0 6 0.0 0.0 0.0 4.072506913080485E-5 0.0 7 0.0 0.0 0.0 4.072506913080485E-5 0.0 8 0.0 0.0 0.0 4.072506913080485E-5 0.0 9 0.0 0.0 0.0 4.072506913080485E-5 0.0 10-11 0.0 0.0 0.0 4.072506913080485E-5 0.0 12-13 0.0 0.0 0.0 1.8326281108862184E-4 0.0 14-15 0.0 0.0 0.0 2.0362534565402426E-4 0.0 16-17 0.0 0.0 0.0 3.665256221772436E-4 0.0 18-19 0.0 0.0 0.0 3.665256221772436E-4 0.0 20-21 0.0 0.0 0.0 4.2761322587345094E-4 0.0 22-23 0.0 0.0 0.0 5.090633641350606E-4 0.0 24-25 4.072506913080485E-5 0.0 0.0 7.534137789198897E-4 0.0 26-27 6.108760369620727E-5 0.0 0.0 0.001099576866531731 2.0362534565402424E-5 28-29 1.8326281108862184E-4 0.0 0.0 0.0034616308761184125 4.072506913080485E-5 30-31 2.0362534565402426E-4 0.0 0.0 0.010629243043140066 4.072506913080485E-5 32-33 2.0362534565402426E-4 0.0 0.0 0.025412443137622226 4.072506913080485E-5 34-35 2.0362534565402426E-4 0.0 0.0 0.04711890498434121 4.072506913080485E-5 36-37 2.0362534565402426E-4 0.0 0.0 0.08538010743273236 4.072506913080485E-5 38-39 2.2398788021942668E-4 0.0 0.0 0.14339296840956386 4.072506913080485E-5 40-41 2.443504147848291E-4 0.0 0.0 0.20285156934053894 4.072506913080485E-5 42-43 2.443504147848291E-4 0.0 0.0 0.26214726999499083 4.072506913080485E-5 44-45 2.443504147848291E-4 0.0 0.0 0.3420498556296299 4.072506913080485E-5 46-47 2.443504147848291E-4 0.0 0.0 0.43934204578312275 6.108760369620727E-5 48-49 2.443504147848291E-4 0.0 0.0 0.5400347792090376 8.14501382616097E-5 50-51 2.443504147848291E-4 0.0 0.0 0.6489539765993753 1.2217520739241455E-4 52-53 2.443504147848291E-4 0.0 0.0 0.7574251982292739 1.2217520739241455E-4 54-55 2.443504147848291E-4 0.0 0.0 0.8778288651144985 1.2217520739241455E-4 56-57 2.443504147848291E-4 0.0 0.0 1.0206720450907967 1.2217520739241455E-4 58-59 2.443504147848291E-4 0.0 0.0 1.1779522620739649 1.2217520739241455E-4 60-61 2.443504147848291E-4 0.0 0.0 1.3515835943131513 1.2217520739241455E-4 62-63 2.443504147848291E-4 0.0 0.0 1.5195337794085906 1.2217520739241455E-4 64-65 2.443504147848291E-4 0.0 0.0 1.6952217276388826 1.2217520739241455E-4 66-67 2.647129493502315E-4 0.0 0.0 1.9042024198836076 1.2217520739241455E-4 68-69 2.8507548391563394E-4 0.0 0.0 2.138941718353567 1.2217520739241455E-4 70-71 2.8507548391563394E-4 0.0 0.0 2.3893398059043207 1.2217520739241455E-4 72-73 2.8507548391563394E-4 0.0 0.0 2.6437493127644585 1.2217520739241455E-4 74-75 2.8507548391563394E-4 0.0 0.0 2.9003376108230943 1.2217520739241455E-4 76-77 2.8507548391563394E-4 0.0 0.0 3.181442400498475 1.2217520739241455E-4 78-79 2.8507548391563394E-4 0.0 0.0 3.499607003082888 1.2217520739241455E-4 80-81 2.8507548391563394E-4 0.0 0.0 3.832819518711133 1.2217520739241455E-4 82-83 2.8507548391563394E-4 0.0 0.0 4.181609373281911 1.2217520739241455E-4 84-85 2.8507548391563394E-4 0.0 0.0 4.536365450480352 1.2217520739241455E-4 86-87 2.8507548391563394E-4 0.0 0.0 4.907065392243503 1.2217520739241455E-4 88-89 2.8507548391563394E-4 0.0 0.0 5.308797836684327 1.2217520739241455E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3460 0.0 55.341263 1 GTATCAA 6120 0.0 40.759422 1 ATCAACG 7905 0.0 31.06471 3 TCAACGC 8050 0.0 30.50454 4 CAACGCA 8255 0.0 29.804544 5 TATCAAC 8275 0.0 29.678131 2 TGGTATC 1590 0.0 28.680593 2 AACGCAG 8650 0.0 28.388617 6 GTGGTAT 1840 0.0 26.080967 1 ACGCAGA 10130 0.0 24.053469 7 CGCAGAG 10195 0.0 23.806934 8 GGACCGA 800 0.0 23.748697 6 GTACATG 7440 0.0 22.224215 1 TACATGG 7285 0.0 22.104675 2 GCAGAGT 11420 0.0 20.920483 9 GAGTACT 6875 0.0 20.657047 12-13 ACATGGG 7600 0.0 20.624289 3 AGAGTAC 10470 0.0 19.801922 10-11 GTACTTT 7300 0.0 19.16162 14-15 CAGAGTA 11035 0.0 19.13239 10-11 >>END_MODULE