FastQCFastQC Report
Thu 26 May 2016
SRR938479_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938479_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2944819
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70750.24025245694217537No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51680.17549465688723143No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37650.12785166083212585No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA100800.032.883561
GGTATCA68950.032.071941
ATCAACG124100.026.2488673
TATCAAC125050.026.0114272
TCAACGC125850.025.7705044
CAACGCA127900.025.1715475
GTACATG99350.025.0345821
AACGCAG132300.024.46076
TACATGG98150.024.370782
ACATGGG102450.023.0229763
ACGCAGA148850.021.5799267
CGCAGAG148500.021.5664238
TAAGGTG16150.020.6118645
GAGTACT78550.020.26243612-13
GCAGAGT155950.019.8045649
AGAGTAC127150.019.65652310-11
GTACTTT82400.019.37205114-15
AGTACTT84200.018.9592112-13
GTAAGGT15200.018.771524
CATGGGG57500.018.3601784
Produced by FastQC (version 0.11.5)