##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938479_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2944819 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.838837633144855 31.0 31.0 34.0 27.0 34.0 2 30.881993426421115 31.0 31.0 34.0 27.0 34.0 3 30.92425986113238 31.0 31.0 34.0 27.0 34.0 4 34.57344067665959 37.0 35.0 37.0 30.0 37.0 5 34.53377881628718 37.0 35.0 37.0 30.0 37.0 6 34.53555583551994 37.0 35.0 37.0 30.0 37.0 7 34.43663973914865 37.0 35.0 37.0 30.0 37.0 8 34.39703289064625 37.0 35.0 37.0 30.0 37.0 9 35.8483709864681 39.0 35.0 39.0 30.0 39.0 10-11 35.6611253526957 38.0 35.0 39.0 30.0 39.0 12-13 35.51132022035989 38.0 35.0 39.0 30.0 39.0 14-15 36.41013046981835 39.0 36.0 40.5 29.0 41.0 16-17 36.21845298471655 38.5 35.5 40.0 28.5 41.0 18-19 36.36934697854096 39.0 36.0 40.5 29.0 41.0 20-21 36.33691985823238 39.0 36.0 41.0 29.0 41.0 22-23 36.242877745627155 39.0 35.0 41.0 27.5 41.0 24-25 36.0951445572716 39.0 35.0 41.0 27.0 41.0 26-27 35.865682746545716 39.0 35.0 40.0 27.0 41.0 28-29 35.64525459799057 38.0 34.0 40.0 27.0 41.0 30-31 35.38415366105693 38.0 34.0 40.0 26.5 41.0 32-33 35.10665290464372 38.0 34.0 40.0 25.0 41.0 34-35 34.83957655801596 38.0 34.0 40.0 25.0 41.0 36-37 34.54504894867902 38.0 33.0 40.0 24.0 41.0 38-39 34.39508455358377 38.0 33.0 40.0 24.0 41.0 40-41 34.291429965644745 38.0 33.0 40.0 24.0 41.0 42-43 33.98194167451378 38.0 33.0 40.0 22.5 41.0 44-45 33.62938910676684 38.0 32.5 40.0 20.5 41.0 46-47 33.240023580396624 37.5 32.0 40.0 18.5 41.0 48-49 32.87425152445702 37.0 31.0 40.0 15.5 41.0 50-51 32.194039599717335 36.5 30.5 39.5 14.5 40.5 52-53 32.234764853120005 36.5 31.0 39.0 13.5 40.0 54-55 32.324975321063874 37.0 31.0 40.0 10.5 41.0 56-57 31.98899423020566 36.5 30.5 39.5 8.0 41.0 58-59 31.59224794461052 36.0 30.0 39.0 7.5 41.0 60-61 31.090733929657475 35.5 29.0 39.0 3.5 40.0 62-63 30.498656284138345 35.0 28.0 38.5 2.0 40.0 64-65 29.95504800125237 34.5 28.0 38.0 2.0 40.0 66-67 29.38778036952356 34.0 27.5 38.0 2.0 40.0 68-69 28.757323964562847 34.0 26.0 37.0 2.0 39.5 70-71 28.092536247558847 33.5 25.5 36.5 2.0 39.0 72-73 27.424339832091547 33.0 24.5 36.0 2.0 39.0 74-75 26.72338792299289 32.5 22.5 35.5 2.0 38.5 76-77 25.939997161115844 32.0 20.5 35.0 2.0 37.5 78-79 25.123272941393004 31.0 18.0 35.0 2.0 37.0 80-81 24.39497368089516 31.0 11.5 34.5 2.0 36.0 82-83 23.622353020678013 30.0 7.0 34.0 2.0 36.0 84-85 22.885813355591633 30.0 2.0 34.0 2.0 35.0 86-87 22.097440793474913 29.0 2.0 34.0 2.0 35.0 88-89 21.243922801367418 29.0 2.0 34.0 2.0 35.0 90-91 20.301950815992427 27.0 2.0 33.5 2.0 35.0 92-93 19.322855666171673 25.5 2.0 33.0 2.0 35.0 94-95 18.298723962321624 24.5 2.0 32.5 2.0 34.0 96-97 17.04153701806461 19.0 2.0 32.0 2.0 34.0 98-99 15.543647843891254 2.0 2.0 32.0 2.0 34.0 100-101 12.269763608561341 2.0 2.0 27.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5710861106819465 1101 2 0.4757168473379316 1101 3 0.5328558832524344 1101 4 0.4636664142089657 1101 5 0.502869281920745 1101 6 0.5657289657645848 1101 7 0.5150309421281278 1101 8 0.581724024428091 1101 9 0.8174950280622042 1101 10-11 0.8253098293673062 1101 12-13 0.8789204600196925 1101 14-15 1.023901160365888 1101 16-17 0.8927414734946595 1101 18-19 1.0514650630784317 1101 20-21 1.0506420659781597 1101 22-23 0.969303259250303 1101 24-25 0.9409369000395387 1101 26-27 0.9690770283700019 1101 28-29 1.129907201233756 1101 30-31 0.9735113878524473 1101 32-33 0.9970117771695897 1101 34-35 0.9217811547478973 1101 36-37 1.0476543507827998 1101 38-39 1.143609868554826 1101 40-41 1.234011263075594 1101 42-43 1.292760269046461 1101 44-45 1.4044327337135982 1101 46-47 1.148820909233983 1101 48-49 1.0874942996240762 1101 50-51 1.1948296887866903 1101 52-53 1.1758552301342604 1101 54-55 1.335689859267248 1101 56-57 1.21980291780541 1101 58-59 1.2627354682319663 1101 60-61 1.3649296079519395 1101 62-63 1.3514973732543858 1101 64-65 1.3114682366616854 1101 66-67 1.3508976105986257 1101 68-69 1.382065251641631 1101 70-71 1.4109586271245753 1101 72-73 1.344176318172952 1101 74-75 1.3083417583830403 1101 76-77 1.3530591175024043 1101 78-79 1.4212155103777242 1101 80-81 1.384973738396603 1101 82-83 1.4577271353256762 1101 84-85 1.599875212564367 1101 86-87 1.8516350852132284 1101 88-89 1.6534090068640133 1101 90-91 1.3894243719722752 1101 92-93 1.3972330748311919 1101 94-95 1.702568524340741 1101 96-97 1.6577414669214114 1101 98-99 1.6250772147511956 1101 100-101 1.3166017981594234 1102 1 0.5619831890101388 1102 2 0.49315112285720275 1102 3 0.4383706445437028 1102 4 0.4265607358101775 1102 5 0.4486291491952912 1102 6 0.47188382248557303 1102 7 0.44500783209313255 1102 8 0.5580446868318987 1102 9 0.7559805006092617 1102 10-11 0.8666435292446835 1102 12-13 0.8188309773919329 1102 14-15 0.7791110931669749 1102 16-17 0.8565976648947853 1102 18-19 0.7514952548628671 1102 20-21 0.7016588310517875 1102 22-23 0.7765178007384748 1102 24-25 0.7351169279274714 1102 26-27 0.6166419647563615 1102 28-29 0.5911139032179094 1102 30-31 0.6798117398679366 1102 32-33 0.8477748227214406 1102 34-35 0.64642172081426 1102 36-37 0.8542550049874578 1102 38-39 0.9038803785567211 1102 40-41 1.1080815522137257 1102 42-43 1.2158935645414033 1102 44-45 1.3545193500951598 1102 46-47 1.3029124140206463 1102 48-49 1.2970297235411508 1102 50-51 1.36801355058725 1102 52-53 1.1192530342739317 1102 54-55 0.9798457633274182 1102 56-57 1.2856647292187375 1102 58-59 1.1683000558341377 1102 60-61 1.1748223753175786 1102 62-63 1.3275127261714346 1102 64-65 1.4591998568579179 1102 66-67 1.1265695689675077 1102 68-69 1.271402125109688 1102 70-71 1.4750622110916503 1102 72-73 1.3333242339901652 1102 74-75 1.3528693987890215 1102 76-77 1.6246551849731503 1102 78-79 1.6829610765143812 1102 80-81 1.8388476477368911 1102 82-83 1.91524582697555 1102 84-85 1.7698180825056085 1102 86-87 1.976013890481891 1102 88-89 1.8748074094127496 1102 90-91 2.001850967579717 1102 92-93 2.1009900650062825 1102 94-95 2.107938000002683 1102 96-97 2.075335285301545 1102 98-99 2.350368791169336 1102 100-101 2.046993109832126 1103 1 0.6020924155529173 1103 2 0.36285285571934267 1103 3 0.4772284567019369 1103 4 0.43236273706698114 1103 5 0.338850747886454 1103 6 0.541309709933266 1103 7 0.46375556961690023 1103 8 0.6346121531244435 1103 9 0.6219593745016212 1103 10-11 0.7891700542704569 1103 12-13 0.8450619860676198 1103 14-15 0.945022622829697 1103 16-17 1.0455463234844657 1103 18-19 0.9303394041202964 1103 20-21 0.8101022665705884 1103 22-23 0.9954984280723735 1103 24-25 1.03435122571355 1103 26-27 1.0387807992819305 1103 28-29 1.1117432543295465 1103 30-31 1.2579436238748656 1103 32-33 1.2778201956266386 1103 34-35 1.330577223935073 1103 36-37 1.234845492417378 1103 38-39 1.4189313706682682 1103 40-41 1.4887192955998714 1103 42-43 1.2840047900889928 1103 44-45 1.3241234086690667 1103 46-47 1.142968970236545 1103 48-49 1.268138186561842 1103 50-51 1.421081510282292 1103 52-53 1.3806474096052952 1103 54-55 1.6558776740947607 1103 56-57 1.5940517091426898 1103 58-59 1.5072351665184982 1103 60-61 1.6824580753777418 1103 62-63 1.7223585807160404 1103 64-65 1.841824446677414 1103 66-67 1.7971756095364775 1103 68-69 2.044700389612238 1103 70-71 1.9258923586907493 1103 72-73 2.1362280855482503 1103 74-75 2.1143794284315014 1103 76-77 2.1485632250021176 1103 78-79 1.987757801634693 1103 80-81 2.2043258720245618 1103 82-83 2.2513435439079394 1103 84-85 2.4686700083325626 1103 86-87 2.4114430883620486 1103 88-89 2.082224162865071 1103 90-91 2.043573185449681 1103 92-93 1.834572902601277 1103 94-95 2.1014807640676487 1103 96-97 2.2586367672818746 1103 98-99 2.5160544625165624 1103 100-101 2.0415765830167363 1104 1 0.5445584762701365 1104 2 0.46967891163750863 1104 3 0.4872193596456782 1104 4 0.4255707433958449 1104 5 0.552532279207405 1104 6 0.5947456069448407 1104 7 0.604337726055924 1104 8 0.776339600906006 1104 9 0.8638599375629781 1104 10-11 0.8686832215583777 1104 12-13 0.8653014422852294 1104 14-15 0.9488042907214123 1104 16-17 1.1052419053673859 1104 18-19 1.0438933284267122 1104 20-21 1.063034446843993 1104 22-23 1.1893851281209606 1104 24-25 1.052564759687769 1104 26-27 1.0642048650639069 1104 28-29 1.0712128577962616 1104 30-31 1.1686708873249785 1104 32-33 1.091550728776653 1104 34-35 1.1787221185742425 1104 36-37 1.364294442486532 1104 38-39 1.3886789468934637 1104 40-41 1.4541012961156312 1104 42-43 1.5378444925109065 1104 44-45 1.3478713682669152 1104 46-47 1.3605405388788725 1104 48-49 1.2870361832328356 1104 50-51 1.3304396184991063 1104 52-53 1.4725883555060761 1104 54-55 1.554398609505668 1104 56-57 1.7433953558440933 1104 58-59 1.794092149669865 1104 60-61 1.738960379061286 1104 62-63 1.812856477912412 1104 64-65 1.810685186329227 1104 66-67 1.8949004720808311 1104 68-69 1.931886089536249 1104 70-71 2.0140683000797814 1104 72-73 1.949994128935554 1104 74-75 1.9255020851580582 1104 76-77 1.8685409857104638 1104 78-79 1.8226239360844936 1104 80-81 2.103267299209726 1104 82-83 2.134494062725146 1104 84-85 2.02556269478659 1104 86-87 2.192053142746726 1104 88-89 2.2156979512534782 1104 90-91 2.1018839829981175 1104 92-93 2.33813691227477 1104 94-95 2.3355557442209864 1104 96-97 2.4183839941506875 1104 98-99 2.6669036743271963 1104 100-101 2.1383369231611393 1105 1 0.4606927154106053 1105 2 0.4040054101889936 1105 3 0.5205317747929961 1105 4 0.43795247797098114 1105 5 0.4918775852176225 1105 6 0.5477913608237373 1105 7 0.4593809257064052 1105 8 0.3648695759398848 1105 9 0.5947616745139328 1105 10-11 0.6437467269020871 1105 12-13 0.7222060898375133 1105 14-15 0.8252626223399844 1105 16-17 0.8084232455742821 1105 18-19 0.8446610095401326 1105 20-21 0.7769166515777925 1105 22-23 0.7176141682431663 1105 24-25 0.9635305347621781 1105 26-27 0.8537955167035847 1105 28-29 0.7495499220708552 1105 30-31 0.9077161460190126 1105 32-33 0.9221269918038999 1105 34-35 0.9684671539901899 1105 36-37 0.9193164644201559 1105 38-39 0.9176602742424649 1105 40-41 1.0002654376584132 1105 42-43 1.0563700451391966 1105 44-45 1.1466520256259187 1105 46-47 0.9107730488543027 1105 48-49 0.9545438595990632 1105 50-51 1.0264891623439922 1105 52-53 0.9607272212658273 1105 54-55 0.9527897815121023 1105 56-57 1.0371223915354477 1105 58-59 1.0922161754559596 1105 60-61 1.1034944548307983 1105 62-63 1.0592976426779401 1105 64-65 1.1778239877994743 1105 66-67 1.476116070804025 1105 68-69 1.2971419761963148 1105 70-71 1.2784217646274634 1105 72-73 1.4865501049557928 1105 74-75 1.5232438665021384 1105 76-77 1.6260397772236281 1105 78-79 1.6831893588791473 1105 80-81 1.9012638026618944 1105 82-83 1.8286968406988109 1105 84-85 1.828512214628578 1105 86-87 1.9738940180093039 1105 88-89 1.6878205012730412 1105 90-91 1.7897521877483769 1105 92-93 1.7089815361267071 1105 94-95 1.854055547681991 1105 96-97 2.097550092630186 1105 98-99 2.054665334250883 1105 100-101 1.8407430076615636 1106 1 0.2810396986879091 1106 2 0.2890119062264809 1106 3 0.19081140095964955 1106 4 0.31176036424775333 1106 5 0.32608731019937665 1106 6 0.17829091676492936 1106 7 0.40966213349776126 1106 8 0.40031354252209894 1106 9 0.3013182709989479 1106 10-11 0.20852618689874447 1106 12-13 0.2248554216618288 1106 14-15 0.5662872058905819 1106 16-17 0.38284658487663137 1106 18-19 0.3155844629911613 1106 20-21 0.4332913446547977 1106 22-23 0.4250099535888623 1106 24-25 0.21662610675421234 1106 26-27 0.21722951330684737 1106 28-29 0.31907508453806344 1106 30-31 0.1903629176435686 1106 32-33 0.4924827833612184 1106 34-35 0.016619673859629813 1106 36-37 0.21961077694411557 1106 38-39 0.19856587808678228 1106 40-41 0.3969380173777637 1106 42-43 -0.01727142815304461 1106 44-45 0.19549451822668829 1106 46-47 0.19982998414056397 1106 48-49 0.5824746560078538 1106 50-51 0.24363706667469387 1106 52-53 0.1850739478604595 1106 54-55 0.3836514654498089 1106 56-57 -0.44274392362470394 1106 58-59 -0.04091167184127187 1106 60-61 0.6739720160273599 1106 62-63 0.6675082187770052 1106 64-65 0.7220111801516573 1106 66-67 0.31177510300212674 1106 68-69 0.4157169433847798 1106 70-71 0.17195219063564693 1106 72-73 0.6461417254816162 1106 74-75 0.9679246097022833 1106 76-77 0.837544197213024 1106 78-79 0.9541026179195597 1106 80-81 1.0397719903861677 1106 82-83 0.9185136295787508 1106 84-85 0.9215580803090972 1106 86-87 0.7156580000631649 1106 88-89 0.6504900164840848 1106 90-91 0.9182874696315615 1106 92-93 0.7265889841653106 1106 94-95 0.46772068570249203 1106 96-97 0.6031891038010748 1106 98-99 0.7620955795761173 1106 100-101 0.27249418982110285 1107 1 0.3840924675817021 1107 2 0.26080735938988653 1107 3 0.36839375061756385 1107 4 0.20509423290981488 1107 5 0.21290862794086962 1107 6 0.3822015357522659 1107 7 0.3708460435182346 1107 8 0.26980085911831253 1107 9 0.4701388370792685 1107 10-11 0.5967546595217357 1107 12-13 0.5642073870619839 1107 14-15 0.7096244026075951 1107 16-17 0.7522446031024472 1107 18-19 0.6546013331945701 1107 20-21 0.8071564646048159 1107 22-23 0.7666120036281185 1107 24-25 0.9304458096701893 1107 26-27 0.7099577931156489 1107 28-29 0.8641032952617635 1107 30-31 0.8591748041565666 1107 32-33 0.9786318446527034 1107 34-35 0.8000249056046087 1107 36-37 0.8716758377026821 1107 38-39 0.9510230742414905 1107 40-41 0.846008659364351 1107 42-43 0.9972401693995963 1107 44-45 0.8653743642607523 1107 46-47 0.8062895630761133 1107 48-49 0.9795132089418743 1107 50-51 0.9323758552562964 1107 52-53 1.0113919721055282 1107 54-55 0.9781288218431712 1107 56-57 1.0589578573053586 1107 58-59 0.9462571587355981 1107 60-61 1.045165373463977 1107 62-63 1.1294887320301754 1107 64-65 1.2036632138488272 1107 66-67 1.3448506443115988 1107 68-69 1.3111325769788138 1107 70-71 1.444724100446102 1107 72-73 1.3680750133688058 1107 74-75 1.4511434916696437 1107 76-77 1.733740246380485 1107 78-79 1.7007793819936943 1107 80-81 1.682496026259301 1107 82-83 1.60540745099415 1107 84-85 1.6843252179550028 1107 86-87 1.7441971096077182 1107 88-89 1.6556081390052846 1107 90-91 1.5355928335296127 1107 92-93 1.793553070671738 1107 94-95 1.8947333522263783 1107 96-97 2.094834088411613 1107 98-99 1.9327022195622536 1107 100-101 1.3454692760549989 1108 1 0.34592731740922034 1108 2 0.18279902268338688 1108 3 0.21183970790512774 1108 4 0.22893961622208536 1108 5 0.1936103202721995 1108 6 0.2892453327629241 1108 7 0.3687767269598936 1108 8 0.27370340554764994 1108 9 0.38513214167755905 1108 10-11 0.4054271198003576 1108 12-13 0.38335949051483453 1108 14-15 0.43028424815499733 1108 16-17 0.5870445656801948 1108 18-19 0.5908621658689626 1108 20-21 0.5776795242882855 1108 22-23 0.4176004282643433 1108 24-25 0.5883567229447451 1108 26-27 0.710177031417949 1108 28-29 0.5774628192875682 1108 30-31 0.6622508274186458 1108 32-33 0.7910178564327666 1108 34-35 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2313 66-67 -1.1591454891054447 2313 68-69 -0.8900866181566371 2313 70-71 -0.9597479755097389 2313 72-73 -1.0752220428757298 2313 74-75 -1.2225433693779664 2313 76-77 -1.2986402152000132 2313 78-79 -1.3698105225227835 2313 80-81 -1.2476883132903822 2313 82-83 -1.4308274081041858 2313 84-85 -1.368594862238016 2313 86-87 -1.3453512664382963 2313 88-89 -1.254301849571977 2313 90-91 -1.24719643227251 2313 92-93 -1.3946087109164509 2313 94-95 -1.597569742174663 2313 96-97 -1.7571870714310585 2313 98-99 -1.6275274468615937 2313 100-101 -1.5424381998550771 2314 1 -0.7048659630008096 2314 2 -0.6945921197639855 2314 3 -0.7539651664155791 2314 4 -0.602567364515437 2314 5 -0.5860220405602234 2314 6 -0.6886071269998624 2314 7 -0.7485616168507789 2314 8 -0.8766901857678135 2314 9 -0.9647523257342883 2314 10-11 -0.9312546395471273 2314 12-13 -0.9662833015850438 2314 14-15 -1.1406292641982887 2314 16-17 -1.3975723637058977 2314 18-19 -1.2484125724143524 2314 20-21 -1.2538817266832964 2314 22-23 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88-89 -2.5667065552432966 2314 90-91 -2.7392932313796123 2314 92-93 -2.7967949994950807 2314 94-95 -2.9538036106510503 2314 96-97 -2.8438943222706445 2314 98-99 -2.9108212922182055 2314 100-101 -2.548888940784053 2315 1 -0.5981137929672933 2315 2 -0.504688175079739 2315 3 -0.5236669109554164 2315 4 -0.4754994084930999 2315 5 -0.4652785538137465 2315 6 -0.5281456421388668 2315 7 -0.6033293846406451 2315 8 -0.6594830732918169 2315 9 -0.6691560039923345 2315 10-11 -0.8035101738026 2315 12-13 -0.8571768461885725 2315 14-15 -1.063768852609222 2315 16-17 -1.032264996154403 2315 18-19 -1.2032206194314838 2315 20-21 -0.9727392233490306 2315 22-23 -0.9816062077491168 2315 24-25 -1.0574008031052031 2315 26-27 -1.3182593387586223 2315 28-29 -1.4283847845500262 2315 30-31 -1.4281215157529132 2315 32-33 -1.5695918048221316 2315 34-35 -1.5168697823886035 2315 36-37 -1.489930056870456 2315 38-39 -1.60267377697825 2315 40-41 -1.6136976752482823 2315 42-43 -1.5609067907241396 2315 44-45 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-0.7054269458924196 2316 6 -0.6887038692784344 2316 7 -0.7973440604211106 2316 8 -0.948184724317116 2316 9 -0.9672263349736738 2316 10-11 -1.0093223671208733 2316 12-13 -1.039691442298178 2316 14-15 -1.1840585685315475 2316 16-17 -1.327642656446038 2316 18-19 -1.1994105138982363 2316 20-21 -1.1205843309455403 2316 22-23 -1.304454583893623 2316 24-25 -1.3247260481441572 2316 26-27 -1.2088075151131292 2316 28-29 -1.3488189294365824 2316 30-31 -1.3067588989533903 2316 32-33 -1.4407464602558875 2316 34-35 -1.3217781200459555 2316 36-37 -1.3684973705734436 2316 38-39 -1.7390224820203954 2316 40-41 -1.5643891774320835 2316 42-43 -1.5159624173252197 2316 44-45 -1.430495173942937 2316 46-47 -1.7423176127540074 2316 48-49 -1.6870391905154811 2316 50-51 -1.7441638508873254 2316 52-53 -1.7268489975157593 2316 54-55 -2.116254130091683 2316 56-57 -2.075975097145566 2316 58-59 -1.971629681810299 2316 60-61 -1.968876296754285 2316 62-63 -2.235929690322383 2316 64-65 -2.304797916985759 2316 66-67 -2.4078046818993784 2316 68-69 -2.5107467009811373 2316 70-71 -2.8935234931131717 2316 72-73 -2.9693741645953544 2316 74-75 -2.8368586567455907 2316 76-77 -2.9728100580639243 2316 78-79 -3.1231316477359776 2316 80-81 -3.213456953023691 2316 82-83 -3.3479319380220254 2316 84-85 -3.4336028039728 2316 86-87 -3.339467180999332 2316 88-89 -3.610052082757999 2316 90-91 -3.597939697194679 2316 92-93 -3.799342113982089 2316 94-95 -4.1741154997784164 2316 96-97 -4.122698089035982 2316 98-99 -4.038326318827318 2316 100-101 -3.2458451494441825 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14093.0 3 5178.0 4 5751.0 5 6102.0 6 7141.0 7 8840.0 8 10826.0 9 13518.0 10 16260.0 11 19620.0 12 23390.0 13 26746.0 14 30115.0 15 33322.0 16 37131.0 17 42187.0 18 41562.0 19 41355.0 20 44112.0 21 48291.0 22 52689.0 23 58045.0 24 64479.0 25 70672.0 26 78817.0 27 88023.0 28 99734.0 29 111461.0 30 127018.0 31 146444.0 32 168247.0 33 196283.0 34 231700.0 35 272775.0 36 305024.0 37 277042.0 38 115975.0 39 4851.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.1664180217053 18.529409667166675 14.441829134895523 23.86234317623251 2 15.47874412656367 19.687017487559636 40.24470753329612 24.589530852580573 3 19.450925782064093 23.59405592872119 29.048046288933214 27.906972000281506 4 12.884788647158219 16.12839944378147 36.641507637805184 34.34530427125513 5 13.985220285900771 37.19264340700062 34.03578980521484 14.786346501883774 6 33.028555151548936 35.61162927216605 17.135980197972685 14.223835378312334 7 29.40895141142763 31.890434968690613 20.28104571833268 18.41956790154908 8 26.475686277040676 35.03383279490034 19.5154816328735 18.974999295185487 9 26.471173651002406 16.70846761264517 19.769967990348196 37.05039074600423 10-11 25.118190026492844 26.323372397326423 27.244769595489338 21.313667980691395 12-13 26.157427856551656 23.514863595776518 27.414969060507673 22.912739487164156 14-15 22.81067146926489 25.09680831478124 25.609807463547423 26.48271275240645 16-17 22.627357364931598 28.26305504813878 26.3656984560093 22.743889130920323 18-19 22.638678596943464 26.971092779428595 28.335398223775236 22.0548303998527 20-21 23.030216538693836 26.15345016959692 28.58371920560855 22.2326140861007 22-23 22.743355000821214 26.102911140879776 28.378039830335606 22.775694027963407 24-25 22.680103436079705 26.237867648432843 28.454893830563112 22.627135084924337 26-27 22.245210385583306 26.64958807336784 28.339597504026848 22.765604037022005 28-29 22.342077384273214 27.092055469216653 28.213528759009805 22.352338387500332 30-31 22.470039590404717 26.528935350964073 28.163965884390446 22.837059174240768 32-33 22.19010190692406 26.527890369703943 28.161343456300703 23.120664267071295 34-35 22.931969693932093 26.595283782135294 28.016088842706317 22.456657681226297 36-37 22.98763427068699 26.177955059348367 27.969125599047302 22.865285070917345 38-39 22.394017224377187 26.650907263988564 27.95618732062187 22.998888191012384 40-41 22.905190195753377 26.421763193581967 27.98368993621641 22.68935667444825 42-43 22.549515158061936 26.450755236517125 27.851032289053162 23.148697316367773 44-45 22.55253533273552 26.66130886107803 27.982127365781622 22.804028440404828 46-47 22.89929001900188 26.913934544540652 27.39850803801735 22.788267398440112 48-49 22.698832087537397 26.856637811293332 27.38357383251205 23.060956268657222 50-51 22.847637250566972 27.031089004944004 27.343425781215508 22.777847963273516 52-53 22.956229553125958 26.86307891843869 27.334191197821312 22.846500330614045 54-55 22.54120839467683 26.995450817375534 27.48465351326293 22.978687274684706 56-57 22.672980608674106 27.005041972283845 27.390080130526474 22.931897288515575 58-59 22.73732240102152 26.617833439784178 27.36541214307197 23.279432016122335 60-61 22.45084011456841 26.99850633089823 27.494125948828035 23.056527605705323 62-63 22.463201226289005 26.95679239910022 27.659850497207756 22.920155877403023 64-65 22.2609623823291 26.97550760779162 27.52340173208307 23.24012827779621 66-67 22.34787193487558 26.774913037535015 27.472990056944813 23.404224970644595 68-69 22.67005380420432 26.560377113387883 27.636563085578146 23.133005996829652 70-71 22.557114665489987 26.627320640222997 27.364935499250052 23.45062919503696 72-73 22.566957776480585 26.617806232991683 27.351867643739467 23.463368346788265 74-75 22.35535610288981 26.971932734488462 27.35772452309324 23.314986639528485 76-77 22.579782861816586 26.569418460413925 27.418807546985242 23.43199113078425 78-79 22.58201669114588 26.667119461400663 27.45464270250537 23.296221144948085 80-81 22.638273985042172 26.778181934261763 27.169480951913606 23.41406312878246 82-83 22.62396594229636 26.684432221899414 27.2520379759968 23.439563859807425 84-85 22.516516709260582 26.665607125010126 27.301467957609503 23.516408208119792 86-87 22.6261362789231 26.760475603976257 27.058071959393082 23.55531615770756 88-89 22.60487758478362 26.717363019631957 26.94175977606185 23.73599961952257 90-91 22.566218138070443 26.549200310557687 27.06145743683241 23.82312411453946 92-93 22.61033114544387 26.266239754777025 27.26814774656896 23.855281353210145 94-95 22.482565646479742 26.09733272609876 27.369450653725618 24.050650973695884 96-97 22.620878919281786 25.874037378326115 27.31628823138524 24.18879547100686 98-99 22.46222668320855 26.041081268358045 26.98271169658726 24.513980351846143 100-101 23.1035155407038 25.337163554357208 27.32580406944558 24.23351683549341 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 593.0 1 406.5 2 297.5 3 388.5 4 359.0 5 364.0 6 585.0 7 1200.5 8 1844.0 9 1978.0 10 1717.0 11 1483.0 12 1513.0 13 1651.5 14 1754.0 15 1894.5 16 2115.0 17 2319.0 18 2559.0 19 2905.0 20 3308.5 21 3879.5 22 4766.0 23 5835.5 24 7080.5 25 9179.0 26 11993.5 27 15510.0 28 19148.0 29 23325.0 30 28483.5 31 35135.5 32 44004.5 33 49573.0 34 53021.0 35 58563.5 36 65742.5 37 72569.0 38 77117.5 39 81484.0 40 86719.0 41 92981.0 42 97437.5 43 101365.5 44 105674.0 45 109854.5 46 112161.5 47 113876.5 48 116327.0 49 114434.5 50 110740.0 51 107040.0 52 103670.5 53 101842.5 54 100572.0 55 95646.0 56 87843.5 57 81093.5 58 75456.5 59 67217.5 60 55529.0 61 46180.0 62 38660.0 63 30072.0 64 22550.0 65 17538.0 66 13531.5 67 10244.5 68 7796.0 69 6020.5 70 4661.0 71 3442.0 72 2520.5 73 1865.5 74 1435.5 75 1101.5 76 775.0 77 544.0 78 384.0 79 229.0 80 143.5 81 95.5 82 58.0 83 36.0 84 28.0 85 23.0 86 12.5 87 10.0 88 9.0 89 5.0 90 4.0 91 3.5 92 3.0 93 2.0 94 1.0 95 1.0 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006519925333271757 2 0.10815605305453407 3 0.11776615133222108 4 0.11983758594331265 5 0.15987400244293454 6 0.09841012299907058 7 0.02764176677751672 8 0.026555112555304756 9 0.024008266721995478 10-11 0.05336490969394044 12-13 0.05620039805502477 14-15 0.02910195838861404 16-17 0.03349951219412806 18-19 0.039391215555183524 20-21 0.06151481636053013 22-23 0.03421262902745466 24-25 0.052702729777280036 26-27 0.05971504530499158 28-29 0.05558915505503055 30-31 0.06997034452711694 32-33 0.052465024166171166 34-35 0.06485967388827632 36-37 0.054468542888374466 38-39 0.03287129022191177 40-41 0.03786310805519796 42-43 0.03304107994413239 44-45 0.006978357583267426 46-47 0.012700271222102275 48-49 0.008455528166586809 50-51 0.00818386461103382 52-53 0.010119467444348872 54-55 0.011341953444337326 56-57 0.014788684805415883 58-59 0.019712586749813826 60-61 0.02467044663865589 62-63 0.011053310916562274 64-65 0.009236560888801655 66-67 0.010238320249903305 68-69 0.011766427749888873 70-71 0.031869530860810125 72-73 0.029051021471947852 74-75 0.029662264471942076 76-77 0.023872434944218982 78-79 0.0048899439999538176 80-81 0.004754112222177322 82-83 0.005755871583278973 84-85 0.005076712694396498 86-87 0.013396409083206811 88-89 0.03901767816629817 90-91 0.03675947486076394 92-93 0.030086738777493627 94-95 0.04823726008287776 96-97 0.04397553805514023 98-99 0.02636834386086208 100-101 0.07660912266594314 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2944819.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.61509074874604 #Duplication Level Percentage of deduplicated Percentage of total 1 74.56842012380224 39.23424191804476 2 13.458507266904249 14.162411623816501 3 4.91034409478536 7.750746004641029 4 2.350969851758739 4.947859683914083 5 1.3060182733374 3.435813498558396 6 0.7645582952631795 2.4136382452787255 7 0.5227776548970584 1.925419562767776 8 0.3614311846709163 1.5213387664709639 9 0.2660753429422108 1.2599620483417329 >10 1.3578040545169348 12.731703273030382 >50 0.08645824025710545 3.139449470369673 >100 0.04130524599751608 4.061848537899532 >500 0.002925203524760795 1.0457661200662354 >1k 0.0022751582968353226 1.9510638512255514 >5k 1.3000904553344702E-4 0.4187373955746239 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7075 0.24025245694217537 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5168 0.17549465688723143 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3765 0.12785166083212585 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 3.395794444412373E-5 0.0 0.0 0.0 12-13 0.0 3.395794444412373E-5 0.0 0.0 0.0 14-15 0.0 3.395794444412373E-5 0.0 1.6978972222061865E-5 3.395794444412373E-5 16-17 0.0 3.395794444412373E-5 0.0 3.395794444412373E-5 3.395794444412373E-5 18-19 0.0 3.395794444412373E-5 0.0 6.791588888824746E-5 3.395794444412373E-5 20-21 0.0 3.395794444412373E-5 0.0 1.0187383333237119E-4 3.395794444412373E-5 22-23 0.0 3.395794444412373E-5 0.0 2.2072663888680425E-4 3.395794444412373E-5 24-25 0.0 3.395794444412373E-5 0.0 7.640537499927839E-4 3.395794444412373E-5 26-27 0.0 3.395794444412373E-5 0.0 0.0014771705833193823 3.395794444412373E-5 28-29 0.0 3.395794444412373E-5 0.0 0.004176827166627218 3.395794444412373E-5 30-31 0.0 3.395794444412373E-5 0.0 0.013583177777649492 3.395794444412373E-5 32-33 0.0 3.395794444412373E-5 0.0 0.03434846080523116 3.395794444412373E-5 34-35 0.0 3.395794444412373E-5 0.0 0.06548789586049261 3.395794444412373E-5 36-37 0.0 3.395794444412373E-5 0.0 0.11501555783224707 3.395794444412373E-5 38-39 0.0 3.395794444412373E-5 0.0 0.1895362669148766 3.395794444412373E-5 40-41 0.0 3.395794444412373E-5 0.0 0.27203709294187517 3.395794444412373E-5 42-43 0.0 3.395794444412373E-5 0.0 0.3571017437744052 3.395794444412373E-5 44-45 0.0 3.395794444412373E-5 0.0 0.46719339966225426 3.395794444412373E-5 46-47 0.0 3.395794444412373E-5 0.0 0.5900532426610939 3.395794444412373E-5 48-49 0.0 3.395794444412373E-5 0.0 0.722642036743175 3.395794444412373E-5 50-51 0.0 3.395794444412373E-5 0.0 0.8676254805473613 3.395794444412373E-5 52-53 0.0 3.395794444412373E-5 0.0 1.0126768402404358 5.09369166661856E-5 54-55 0.0 3.395794444412373E-5 0.0 1.1703605552667242 6.791588888824746E-5 56-57 0.0 3.395794444412373E-5 0.0 1.3571292497094047 6.791588888824746E-5 58-59 0.0 3.395794444412373E-5 0.0 1.5601807785130428 6.791588888824746E-5 60-61 0.0 3.395794444412373E-5 0.0 1.7783945295109818 6.791588888824746E-5 62-63 0.0 3.395794444412373E-5 0.0 1.9909373037867524 6.791588888824746E-5 64-65 0.0 3.395794444412373E-5 0.0 2.2212231040345776 6.791588888824746E-5 66-67 3.395794444412373E-5 3.395794444412373E-5 0.0 2.485093311337641 6.791588888824746E-5 68-69 3.395794444412373E-5 3.395794444412373E-5 0.0 2.778099434973762 6.791588888824746E-5 70-71 3.395794444412373E-5 3.395794444412373E-5 0.0 3.0837209349708763 6.791588888824746E-5 72-73 3.395794444412373E-5 3.395794444412373E-5 0.0 3.4022124959123126 6.791588888824746E-5 74-75 3.395794444412373E-5 3.395794444412373E-5 0.0 3.7195664657148706 6.791588888824746E-5 76-77 3.395794444412373E-5 3.395794444412373E-5 0.0 4.064375433600503 8.489486111030932E-5 78-79 3.395794444412373E-5 3.395794444412373E-5 0.0 4.4538730563746025 1.0187383333237119E-4 80-81 3.395794444412373E-5 3.395794444412373E-5 0.0 4.861062768204089 1.0187383333237119E-4 82-83 3.395794444412373E-5 3.395794444412373E-5 0.0 5.2786945479501455 1.0187383333237119E-4 84-85 3.395794444412373E-5 3.395794444412373E-5 0.0 5.705868510085001 1.0187383333237119E-4 86-87 3.395794444412373E-5 3.395794444412373E-5 0.0 6.151498615025236 1.0187383333237119E-4 88-89 3.395794444412373E-5 3.395794444412373E-5 0.0 6.625059129270763 1.0187383333237119E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10080 0.0 32.88356 1 GGTATCA 6895 0.0 32.07194 1 ATCAACG 12410 0.0 26.248867 3 TATCAAC 12505 0.0 26.011427 2 TCAACGC 12585 0.0 25.770504 4 CAACGCA 12790 0.0 25.171547 5 GTACATG 9935 0.0 25.034582 1 AACGCAG 13230 0.0 24.4607 6 TACATGG 9815 0.0 24.37078 2 ACATGGG 10245 0.0 23.022976 3 ACGCAGA 14885 0.0 21.579926 7 CGCAGAG 14850 0.0 21.566423 8 TAAGGTG 1615 0.0 20.611864 5 GAGTACT 7855 0.0 20.262436 12-13 GCAGAGT 15595 0.0 19.804564 9 AGAGTAC 12715 0.0 19.656523 10-11 GTACTTT 8240 0.0 19.372051 14-15 AGTACTT 8420 0.0 18.95921 12-13 GTAAGGT 1520 0.0 18.77152 4 CATGGGG 5750 0.0 18.360178 4 >>END_MODULE