FastQCFastQC Report
Thu 26 May 2016
SRR938479_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938479_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2944819
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69920.23743394755331312No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57680.19586942355370568No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40900.13888799277646607No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA103300.033.21311
GGTATCA71100.032.7490771
ATCAACG124750.027.2617153
TCAACGC125650.027.104254
TATCAAC128750.026.5002372
CAACGCA128500.026.466145
AACGCAG132550.025.6470246
GTACATG99200.023.2328591
TACATGG98050.022.7299162
ACGCAGA149000.022.719937
CGCAGAG148900.022.6395268
ACATGGG98900.021.419993
GAGTACT80850.020.72995612-13
GGACCGA12000.020.5743376
TATATCG1856.559507E-620.539935
GTACTTT83000.020.19297414-15
GCAGAGT162500.020.1019789
AGAGTAC130000.019.99606110-11
GTAAGGT15450.019.9832274
TAAGGTG16950.019.3357095
Produced by FastQC (version 0.11.5)