##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938479_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2944819 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.784218996142037 31.0 31.0 34.0 30.0 34.0 2 32.00866097373047 31.0 31.0 34.0 30.0 34.0 3 32.074440907913186 33.0 31.0 34.0 30.0 34.0 4 35.7508668614268 37.0 35.0 37.0 35.0 37.0 5 35.64702550479333 37.0 35.0 37.0 33.0 37.0 6 35.64274374757837 37.0 35.0 37.0 33.0 37.0 7 35.57610739403678 37.0 35.0 37.0 33.0 37.0 8 35.55326931808033 37.0 35.0 37.0 33.0 37.0 9 37.13059682106099 39.0 37.0 39.0 33.0 39.0 10-11 37.0306957745111 39.0 37.0 39.0 33.0 39.0 12-13 36.926956461500694 39.0 37.0 39.0 33.0 39.0 14-15 37.977182638389664 40.0 37.0 41.0 33.0 41.0 16-17 37.89018832736409 40.0 37.0 41.0 33.0 41.0 18-19 38.11674367762501 40.0 37.0 41.0 33.0 41.0 20-21 38.08154066514784 40.0 37.0 41.0 33.0 41.0 22-23 37.98034395322769 40.0 37.0 41.0 33.0 41.0 24-25 37.864053104791836 40.0 37.0 41.0 32.0 41.0 26-27 37.67341184636475 40.0 37.0 41.0 32.0 41.0 28-29 37.56503948120411 39.5 37.0 41.0 32.0 41.0 30-31 37.32762862505302 39.0 36.0 41.0 31.0 41.0 32-33 37.14415079500641 39.0 36.0 41.0 31.0 41.0 34-35 37.0604830042186 39.0 36.0 41.0 31.0 41.0 36-37 37.02564894480781 39.0 36.0 41.0 31.0 41.0 38-39 36.86666226345321 39.0 35.5 41.0 30.0 41.0 40-41 36.70484807385445 39.0 35.0 41.0 30.0 41.0 42-43 36.503428054491636 39.0 35.0 40.0 30.0 41.0 44-45 36.225524556857316 39.0 35.0 40.0 30.0 41.0 46-47 35.87983879484614 38.0 35.0 40.0 28.5 41.0 48-49 35.556435387030575 38.0 34.5 40.0 27.5 41.0 50-51 35.19444981168622 38.0 34.0 40.0 27.0 41.0 52-53 34.71018948872579 38.0 33.0 40.0 26.0 41.0 54-55 34.384493410291086 38.0 33.0 40.0 24.5 41.0 56-57 34.059885174606656 37.0 33.0 40.0 24.0 41.0 58-59 33.596581487690756 37.0 32.0 39.5 23.0 41.0 60-61 33.20208508570475 36.0 32.0 39.0 22.0 40.0 62-63 32.717446810822665 36.0 31.0 39.0 21.5 40.0 64-65 32.18916578574099 35.0 31.0 38.5 19.5 40.0 66-67 31.694842365523996 35.0 30.0 38.0 18.0 40.0 68-69 31.200000747074778 34.5 30.0 38.0 16.5 40.0 70-71 30.627846057771293 34.0 29.0 37.0 12.5 39.0 72-73 30.122231621026625 34.0 29.0 36.5 8.0 39.0 74-75 29.52016745341564 34.0 28.0 36.0 7.0 39.0 76-77 28.29853023224857 31.5 26.5 34.5 7.0 36.5 78-79 28.992139924389242 33.0 28.0 35.0 4.0 37.0 80-81 28.611481214974503 33.0 27.5 35.0 2.0 37.0 82-83 28.162824438445963 33.0 26.5 35.0 2.0 36.0 84-85 27.595257807016324 33.0 26.0 35.0 2.0 36.0 86-87 26.9848484745582 32.0 25.5 35.0 2.0 35.5 88-89 26.234313552038344 32.0 24.5 34.0 2.0 35.0 90-91 25.62146739748691 31.0 22.0 34.0 2.0 35.0 92-93 24.78747556301423 31.0 19.0 34.0 2.0 35.0 94-95 23.974265141592742 31.0 12.0 34.0 2.0 35.0 96-97 22.8987509928454 30.0 2.0 34.0 2.0 35.0 98-99 21.53052530562999 29.5 2.0 34.0 2.0 35.0 100-101 17.578201750260373 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23516520000566032 1101 2 0.2979450876513212 1101 3 0.27881092613115044 1101 4 0.2281978051192226 1101 5 0.11689101562676996 1101 6 0.19245171387898097 1101 7 0.18351047192644643 1101 8 0.2543235102698347 1101 9 0.3556646191199704 1101 10-11 0.17167761028149897 1101 12-13 0.32610215888490757 1101 14-15 0.3715181199931692 1101 16-17 0.4130168805284029 1101 18-19 0.4004815449401491 1101 20-21 0.35196269845833683 1101 22-23 0.2868787879001573 1101 24-25 0.3818929588672191 1101 26-27 0.32433878123861604 1101 28-29 0.17998255159159982 1101 30-31 0.37847694056728187 1101 32-33 0.3807938323654909 1101 34-35 0.41845331833151533 1101 36-37 0.44255722722660096 1101 38-39 0.41561763600838475 1101 40-41 0.6372600765113319 1101 42-43 0.693944208565874 1101 44-45 0.60339027309373 1101 46-47 0.5853464029631752 1101 48-49 0.5864981899827555 1101 50-51 0.7372161529305359 1101 52-53 0.6517667617303289 1101 54-55 0.657378773807487 1101 56-57 0.6214904771720029 1101 58-59 0.8559939578415339 1101 60-61 0.7169051115782636 1101 62-63 0.7897674896155138 1101 64-65 0.6746649955384569 1101 66-67 0.6692663580613818 1101 68-69 0.6887233757067364 1101 70-71 0.5850793965290961 1101 72-73 0.739197632950269 1101 74-75 0.6219998021494035 1101 76-77 0.640752301214615 1101 78-79 0.7558145640089329 1101 80-81 0.7743903406778436 1101 82-83 0.762625372245612 1101 84-85 0.9176848827866912 1101 86-87 0.8908534871244136 1101 88-89 1.1047330805177147 1101 90-91 1.3080334508829488 1101 92-93 1.4621165185429454 1101 94-95 1.2092411994582264 1101 96-97 1.2901280237350896 1101 98-99 1.5429378598866208 1101 100-101 1.7066379127124272 1102 1 0.29522995447664613 1102 2 0.26228128895751013 1102 3 0.2015662076584448 1102 4 0.18389086813742495 1102 5 0.10128674704124307 1102 6 0.1252949733781108 1102 7 0.10421473476467469 1102 8 0.16879159096773577 1102 9 0.19529407416566613 1102 10-11 0.2416021160205375 1102 12-13 0.2503827545214605 1102 14-15 0.4255112178157887 1102 16-17 0.34163606518742995 1102 18-19 0.26419351137004554 1102 20-21 0.2475882320253575 1102 22-23 0.33811497374045985 1102 24-25 0.3343909713647477 1102 26-27 0.27395500764541936 1102 28-29 0.29661182385859064 1102 30-31 0.2538565886899278 1102 32-33 0.38792861475647555 1102 34-35 0.2156568412983617 1102 36-37 0.29527367383116854 1102 38-39 0.3665402614208446 1102 40-41 0.31375461300700636 1102 42-43 0.2747372633701346 1102 44-45 0.37816548119874227 1102 46-47 0.3496963188381059 1102 48-49 0.34022597503303587 1102 50-51 0.5067287652771455 1102 52-53 0.3840234767507198 1102 54-55 0.29928361394025416 1102 56-57 0.4051416752357824 1102 58-59 0.41990335486177344 1102 60-61 0.25993999764251186 1102 62-63 0.32190040351984095 1102 64-65 0.500232269321458 1102 66-67 0.40638742835026775 1102 68-69 0.3885769554188485 1102 70-71 0.3691377150193631 1102 72-73 0.5207236278546681 1102 74-75 0.6532457707198525 1102 76-77 0.5151436247272336 1102 78-79 0.8043998421100476 1102 80-81 0.8341033509936153 1102 82-83 0.8726722065112149 1102 84-85 0.8107327064252274 1102 86-87 0.6719947464424898 1102 88-89 0.8753776381155731 1102 90-91 0.75585068846787 1102 92-93 1.1008887143761363 1102 94-95 0.9699709378478936 1102 96-97 0.911393362900565 1102 98-99 1.3073551152807497 1102 100-101 1.6790385764383515 1103 1 0.2657106140332388 1103 2 0.27876421440426213 1103 3 0.2344684516755038 1103 4 0.28837408682265675 1103 5 0.07429515105487638 1103 6 0.13216834921664855 1103 7 0.17997962841661064 1103 8 0.13662052656236057 1103 9 0.3116607583451838 1103 10-11 0.2803458310981384 1103 12-13 0.36208601251118466 1103 14-15 0.48058536953843145 1103 16-17 0.44801383445234677 1103 18-19 0.46664940760770435 1103 20-21 0.35855300339876806 1103 22-23 0.38094863493071074 1103 24-25 0.47657563877744025 1103 26-27 0.538314409735996 1103 28-29 0.4839943278229413 1103 30-31 0.39392438092279747 1103 32-33 0.4452281087523957 1103 34-35 0.48877788099168384 1103 36-37 0.3862370173440013 1103 38-39 0.464295884999423 1103 40-41 0.5089696270089021 1103 42-43 0.5681897440023747 1103 44-45 0.5200254660528785 1103 46-47 0.48715856498258603 1103 48-49 0.5898506824833305 1103 50-51 0.6283558339680297 1103 52-53 0.4099141107759223 1103 54-55 0.5169731175119452 1103 56-57 0.560418099221252 1103 58-59 0.6474397659054603 1103 60-61 0.5982202929029938 1103 62-63 0.5296448940982401 1103 64-65 0.6318813740217308 1103 66-67 0.6787265586538105 1103 68-69 0.734329501430814 1103 70-71 0.6100098287824807 1103 72-73 0.5375398006277123 1103 74-75 0.6694737296195079 1103 76-77 0.5679637451993749 1103 78-79 0.8114430758596889 1103 80-81 0.8598023857742412 1103 82-83 0.9902077718589553 1103 84-85 0.9387368136805563 1103 86-87 0.8896922158471021 1103 88-89 0.8812820572242757 1103 90-91 1.0958637620168261 1103 92-93 1.4221684248366735 1103 94-95 1.3786818935873306 1103 96-97 1.3467083439131358 1103 98-99 1.516792614876465 1103 100-101 1.6438833193599613 1104 1 0.16803608638834433 1104 2 0.21855930769390852 1104 3 0.2545392055360054 1104 4 0.13419461233739582 1104 5 0.0841704446176621 1104 6 0.11289720775443612 1104 7 0.19385459284891482 1104 8 0.14814032334727045 1104 9 0.2641158832883548 1104 10-11 0.28821473879404635 1104 12-13 0.2568973347837158 1104 14-15 0.4250185135298068 1104 16-17 0.4201587539142082 1104 18-19 0.2721193861728537 1104 20-21 0.3909468438780479 1104 22-23 0.322401562146446 1104 24-25 0.29366976136200407 1104 26-27 0.3602335576373932 1104 28-29 0.33602822550473377 1104 30-31 0.3281844739895021 1104 32-33 0.4455895317177081 1104 34-35 0.5040187829011487 1104 36-37 0.40815625718128956 1104 38-39 0.4045015869552131 1104 40-41 0.43149295459164705 1104 42-43 0.5560135249669571 1104 44-45 0.458610797957526 1104 46-47 0.4071480245328587 1104 48-49 0.3221126868971709 1104 50-51 0.4938817831026512 1104 52-53 0.38475677399315344 1104 54-55 0.39497373256304513 1104 56-57 0.3963094337580273 1104 58-59 0.47581970843633314 1104 60-61 0.5523668083719002 1104 62-63 0.4406639390774103 1104 64-65 0.4798397537252299 1104 66-67 0.5713498067579756 1104 68-69 0.6079986719714299 1104 70-71 0.8376799802790345 1104 72-73 0.6844941484827807 1104 74-75 0.7624289582203971 1104 76-77 0.7002507561751408 1104 78-79 0.8463935385040635 1104 80-81 0.8517207614141711 1104 82-83 0.9977360570845342 1104 84-85 1.1694834847969204 1104 86-87 1.2794290217828497 1104 88-89 1.132982526419088 1104 90-91 1.0890051148258593 1104 92-93 1.4397702403866184 1104 94-95 1.4233142396671141 1104 96-97 1.4129888338762662 1104 98-99 1.7259486020403791 1104 100-101 2.014524121702852 1105 1 0.16601552754132243 1105 2 0.1803153986788928 1105 3 0.2388263184281385 1105 4 0.15829193996312085 1105 5 0.13778966526433578 1105 6 0.15033438367920127 1105 7 0.15217953777461446 1105 8 0.17404231723376284 1105 9 0.17168360248606973 1105 10-11 0.15296543012860297 1105 12-13 0.2331855216886396 1105 14-15 0.27637941541579636 1105 16-17 0.2659355345497687 1105 18-19 0.27922565582257874 1105 20-21 0.23103016662622622 1105 22-23 0.17069536559542797 1105 24-25 0.23320159589729172 1105 26-27 0.15855879397916084 1105 28-29 0.23807044034688118 1105 30-31 0.39669833660706644 1105 32-33 0.3879592804169576 1105 34-35 0.3807098024591653 1105 36-37 0.48313655322886007 1105 38-39 0.2983821499710473 1105 40-41 0.27278465294887866 1105 42-43 0.3730547910384985 1105 44-45 0.34333433420913195 1105 46-47 0.3388607323419919 1105 48-49 0.4207052474232853 1105 50-51 0.450367136331181 1105 52-53 0.46937621242327765 1105 54-55 0.4565612360194393 1105 56-57 0.42626822841670275 1105 58-59 0.3764021735092271 1105 60-61 0.4797591618368955 1105 62-63 0.4106244488642119 1105 64-65 0.5028997180301005 1105 66-67 0.333268668073071 1105 68-69 0.3990175403828431 1105 70-71 0.5622744503589487 1105 72-73 0.7037881719397703 1105 74-75 0.6773914712145483 1105 76-77 0.45809540961359474 1105 78-79 0.7439035662197355 1105 80-81 0.6678129231896826 1105 82-83 0.7306975605778554 1105 84-85 0.7806006516277222 1105 86-87 0.7180779880010562 1105 88-89 0.7458464099807962 1105 90-91 0.8124076153536315 1105 92-93 0.9853372014576607 1105 94-95 1.1318154245737233 1105 96-97 1.1785778135440985 1105 98-99 1.1281117211799412 1105 100-101 1.3951965853987538 1106 1 -0.02337000353059082 1106 2 0.08389957894169697 1106 3 0.10352804466326404 1106 4 0.06331526616626348 1106 5 -0.03634610992671128 1106 6 -0.04844535751972501 1106 7 0.07938426683247712 1106 8 0.14955673459337504 1106 9 -0.18677517934703047 1106 10-11 0.08679254993369767 1106 12-13 -0.007485957520344755 1106 14-15 0.03836466809083561 1106 16-17 0.11131923924276066 1106 18-19 0.025130799648259483 1106 20-21 0.14357123557700646 1106 22-23 0.19905946311231304 1106 24-25 0.1831807266244283 1106 26-27 0.20044876481077978 1106 28-29 0.15194307825058928 1106 30-31 0.0869675673465693 1106 32-33 0.1524780042799705 1106 34-35 0.18001918912565884 1106 36-37 0.3026062428604206 1106 38-39 0.14922245988554295 1106 40-41 0.17302511070995052 1106 42-43 0.18114953963583247 1106 44-45 0.09995537196036963 1106 46-47 -0.643863662595443 1106 48-49 -0.11465753939943824 1106 50-51 0.25954118915943525 1106 52-53 0.13481807716762972 1106 54-55 0.19234591460447348 1106 56-57 0.20232491552484078 1106 58-59 0.12391401388088497 1106 60-61 -0.03482690809190103 1106 62-63 -0.22587103306799605 1106 64-65 0.019007107696587866 1106 66-67 -0.11630710417562184 1106 68-69 -0.12628425779853458 1106 70-71 -0.056690787142652965 1106 72-73 0.30293787194466404 1106 74-75 0.36295603115785013 1106 76-77 0.24708419808134252 1106 78-79 0.5822586500220979 1106 80-81 -0.006683853421623809 1106 82-83 0.45240815396213563 1106 84-85 0.3390702353390189 1106 86-87 0.4979157190499741 1106 88-89 0.6976460106205593 1106 90-91 0.6382247338123541 1106 92-93 0.6983271558213815 1106 94-95 0.720423573850816 1106 96-97 0.9478343980233461 1106 98-99 0.9563362889142901 1106 100-101 1.258627876010035 1107 1 0.19712902579559 1107 2 0.16927925782752595 1107 3 0.11300496068839294 1107 4 0.0548988548190934 1107 5 0.05816841418432972 1107 6 0.06639522225832906 1107 7 0.11920377869147813 1107 8 0.12914313776896336 1107 9 0.3535744251043198 1107 10-11 0.2181630727812447 1107 12-13 0.2623880417867781 1107 14-15 0.347096398001014 1107 16-17 0.26832707396334854 1107 18-19 0.24836351993435102 1107 20-21 0.24339690939630998 1107 22-23 0.2853047557782702 1107 24-25 0.24912957000019986 1107 26-27 0.2979337776474793 1107 28-29 0.26146787566788277 1107 30-31 0.2948594452843665 1107 32-33 0.3129247048675978 1107 34-35 0.39903571640628144 1107 36-37 0.21220018927223805 1107 38-39 0.2716965859889058 1107 40-41 0.3452859803770352 1107 42-43 0.39040820980471125 1107 44-45 0.36649103928437654 1107 46-47 0.4096261677738582 1107 48-49 0.43644998936374435 1107 50-51 0.4885361836582902 1107 52-53 0.5104184183438534 1107 54-55 0.2539543353396354 1107 56-57 0.43715726515613085 1107 58-59 0.3665609560267242 1107 60-61 0.3287416890844739 1107 62-63 0.5632465254410732 1107 64-65 0.3680821054414949 1107 66-67 0.4403128550591653 1107 68-69 0.7642773677150885 1107 70-71 0.7527406862889805 1107 72-73 0.8097096916580178 1107 74-75 0.6418611468735236 1107 76-77 0.7820196162431934 1107 78-79 1.0267938187236005 1107 80-81 0.9922679423915035 1107 82-83 1.145071118695725 1107 84-85 1.2315870531987798 1107 86-87 1.3630774161939918 1107 88-89 1.2020255179384982 1107 90-91 1.4147381761191617 1107 92-93 1.407969430915955 1107 94-95 1.4927158710064035 1107 96-97 1.4933645326823068 1107 98-99 1.602964905351854 1107 100-101 1.6246407032390415 1108 1 0.1609683102017705 1108 2 0.09206153943792117 1108 3 0.11323759398005251 1108 4 0.12848454277528276 1108 5 0.06307956145261073 1108 6 0.10408003648447561 1108 7 0.1557072176456984 1108 8 0.14880091253473182 1108 9 0.2038361297088045 1108 10-11 0.1734918297722814 1108 12-13 0.1650913913294474 1108 14-15 0.27897780169544717 1108 16-17 0.24301467769603846 1108 18-19 0.27213203903037453 1108 20-21 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-1.1576341759895747 2316 80-81 -1.0603664850383794 2316 82-83 -1.2222818016393546 2316 84-85 -1.402427895334764 2316 86-87 -1.3869177570604982 2316 88-89 -1.3677416480738174 2316 90-91 -1.398531676952647 2316 92-93 -1.514082018083318 2316 94-95 -1.604253086858165 2316 96-97 -1.6578618201158193 2316 98-99 -1.5563647829328104 2316 100-101 -2.1535762456210783 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 13.0 4 42.0 5 77.0 6 208.0 7 506.0 8 1198.0 9 2133.0 10 3510.0 11 5514.0 12 7816.0 13 11079.0 14 14332.0 15 18637.0 16 23284.0 17 28495.0 18 27646.0 19 25317.0 20 25396.0 21 27687.0 22 30594.0 23 34545.0 24 39762.0 25 47270.0 26 55906.0 27 67258.0 28 80076.0 29 95061.0 30 112545.0 31 133905.0 32 160808.0 33 195636.0 34 246197.0 35 318482.0 36 413204.0 37 459475.0 38 222174.0 39 9026.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.06631139672161 19.381262515692903 15.005859475149952 23.546566612435534 2 15.95525537656785 20.466785196520483 39.38275006701705 24.19520935989462 3 19.482283970593777 23.887172692107733 29.56429580222078 27.066247535077707 4 13.438822555817524 16.8715632437851 36.35466220504554 33.33495199535184 5 13.843832682630516 37.39568484150075 34.26942358103526 14.49105889483347 6 33.04755439811709 36.14268730450823 16.818871936694197 13.990886360680477 7 29.333313864111854 32.07178437791932 20.2599888142531 18.334912943715725 8 26.327967864918012 35.64874445594109 19.319693332595314 18.703594346545575 9 26.206602171474714 16.79094708367475 19.774831661979906 37.227619082870625 10-11 25.09405501662411 26.56385672599912 27.203352735770856 21.138735521605913 12-13 26.069513949753787 23.57333336955514 27.525138217323374 22.8320144633677 14-15 22.663684932758176 25.223417805984 25.648588249396653 26.46430901186117 16-17 22.512300416426275 28.48657251939763 26.452593521027946 22.548533543148153 18-19 22.542743713620432 27.11433198441059 28.452274995509065 21.890649306459924 20-21 22.874750536450627 26.223547185752334 28.80852779067236 22.093174487124674 22-23 22.77185796478493 26.14919626639192 28.537865994480477 22.541079774342666 24-25 22.75608317352314 26.357050898259516 28.554518907071465 22.332347021145885 26-27 22.25745430144635 26.74080854480646 28.50360105808301 22.49813609566418 28-29 22.341973148746817 27.09726176440226 28.367442991344788 22.193322095506137 30-31 22.44467970045326 26.57827870139815 28.279592805241133 22.69744879290746 32-33 22.2359639310705 26.50882604830335 28.31821518972727 22.936994830898875 34-35 22.873234452363963 26.626983412707766 28.20530802860808 22.29447410632019 36-37 22.974911532536513 26.22086118577395 28.1750848246606 22.629142457028937 38-39 22.429424922937848 26.62144645585409 28.165117612959683 22.784011008248374 40-41 22.908845298162976 26.38029768161776 28.16616326356663 22.544693756652638 42-43 22.565418668441556 26.526814798716035 27.972320536406336 22.93544599643607 44-45 22.582268146689856 26.667704727324843 28.14359605154328 22.606431074442025 46-47 22.93536546728339 26.90287586435703 27.5095345418513 22.652224126508287 48-49 22.681224211063565 26.8614471721352 27.53666693946215 22.920661677339083 50-51 22.896429428263332 26.95830645693939 27.48737311781055 22.657890996986733 52-53 23.073659196032082 26.794244400080274 27.41820465026883 22.713891753618814 54-55 22.679119855147466 26.929239048787213 27.561304922459612 22.830336173605705 56-57 22.710699706841066 26.985308774495138 27.46813980757391 22.835851711089884 58-59 22.796260082382798 26.64693849650964 27.378075238202793 23.178726182904764 60-61 22.473843044343305 27.019521403522596 27.571575706350714 22.93505984578339 62-63 22.499339883836537 27.02159832164935 27.68409175232611 22.794970042188005 64-65 22.243479179342586 27.00900745161443 27.52218579331479 23.225327575728194 66-67 22.299083063875138 26.909624204084466 27.45269503761661 23.33859769442379 68-69 22.61398890656679 26.673418571031444 27.57658766972523 23.136004852676535 70-71 22.568685931927178 26.75962547078477 27.26052247049647 23.41116612679158 72-73 22.576484764680743 26.848276048252213 27.195614941973506 23.379624245093545 74-75 22.336559779835145 27.168926934105937 27.14705310432611 23.347460181732803 76-77 22.472645535299154 26.79386189101906 27.357233655058632 23.37625891862315 78-79 22.506892086702383 26.90598976343396 27.36538861314503 23.22172953671862 80-81 22.504069652408802 27.03850736103952 27.15326434101352 23.30415864553816 82-83 22.576122213373022 26.9584795956341 27.182749872230143 23.28264831876273 84-85 22.44503744097205 27.001159501801403 27.15542011052966 23.39838294669688 86-87 22.54633784365483 27.121428880717463 26.878912428671008 23.4533208469567 88-89 22.41469901908205 27.188236869491217 26.68980806472114 23.707256046705588 90-91 22.3333439760227 27.03723152400821 26.933516251726324 23.695908248242766 92-93 22.332985351652557 26.88403888359868 27.042643125510658 23.7403326392381 94-95 22.222197690592367 26.74653410528711 27.080903994334328 23.950364209786194 96-97 22.22227695284574 26.53910770097807 27.103888103126987 24.134727243049205 98-99 22.05250675314222 26.78132865960341 26.765835632230186 24.400328955024182 100-101 22.20034091592201 26.63968569712008 27.03103598296575 24.12893740399216 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 544.0 1 377.0 2 311.5 3 410.0 4 370.0 5 363.5 6 581.5 7 1222.0 8 1916.5 9 2086.0 10 1751.5 11 1431.5 12 1435.0 13 1567.5 14 1731.5 15 1875.0 16 2045.0 17 2274.5 18 2527.0 19 2841.0 20 3195.5 21 3773.0 22 4626.5 23 5566.5 24 6918.5 25 8956.5 26 11641.0 27 15178.0 28 18956.5 29 23282.5 30 28524.0 31 35489.0 32 45498.0 33 51381.0 34 54078.0 35 59450.0 36 66564.5 37 73260.5 38 77666.0 39 81876.5 40 87137.5 41 93820.5 42 98453.0 43 102923.5 44 107793.0 45 111853.0 46 114136.0 47 116786.5 48 119209.0 49 116529.0 50 112900.5 51 108434.5 52 104124.5 53 102582.5 54 102340.5 55 96753.0 56 87740.5 57 80116.0 58 73525.0 59 65029.0 60 52952.5 61 43632.5 62 36355.5 63 27391.0 64 19645.5 65 14845.0 66 11227.0 67 8491.5 68 6439.0 69 4865.5 70 3740.0 71 2708.5 72 1914.0 73 1511.0 74 1182.5 75 876.5 76 606.5 77 389.5 78 263.0 79 141.5 80 77.0 81 49.0 82 25.0 83 13.5 84 8.5 85 1.5 86 2.5 87 3.0 88 0.5 89 0.0 90 0.0 91 0.0 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.433271111059797E-4 2 0.0522273185550623 3 0.0 4 0.0 5 0.04533385583290518 6 6.791588888824746E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006112429999942271 26-27 0.002020497694425362 28-29 1.018738333323712E-4 30-31 0.001239464972210516 32-33 4.414532777736085E-4 34-35 0.011409869333225573 36-37 0.022327348472011354 38-39 0.004007037444406601 40-41 9.678014166575263E-4 42-43 0.001239464972210516 44-45 0.007657516472149902 46-47 0.0 48-49 0.0 50-51 3.5655841666329917E-4 52-53 0.0 54-55 0.0010526962777678355 56-57 0.0 58-59 0.021614231638684755 60-61 0.0 62-63 0.008880002472138356 64-65 0.0143811894720864 66-67 0.005959619249943715 68-69 0.015773465194295472 70-71 6.791588888824746E-5 72-73 1.6978972222061865E-5 74-75 0.02246318024978785 76-77 0.04990119936063983 78-79 0.029305706055278775 80-81 0.02619855413864146 82-83 0.035638862694107855 84-85 0.015315032944299804 86-87 0.009389371638800211 88-89 0.08397799661031799 90-91 0.023804519055330736 92-93 0.023719624194220426 94-95 0.02628344899975177 96-97 0.037642381416311155 98-99 0.05295741436061095 100-101 0.013515261888761244 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2944819.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.49139313276643 #Duplication Level Percentage of deduplicated Percentage of total 1 70.83534119028609 34.349043773518055 2 15.074566719138428 14.619734821677168 3 5.7114591614468475 8.308698346783787 4 2.7942256917405555 5.4198358607947 5 1.5160702175666207 3.6758178468450873 6 0.9330369657063332 2.7146557386881587 7 0.6249827377078506 2.1214398544768818 8 0.44315432874021243 1.7191336618743083 9 0.31240151252390025 1.363390610316057 >10 1.5948391609600008 13.897277531973995 >50 0.10409382775495403 3.4775028267862163 >100 0.04936543549698114 4.474225854832978 >500 0.0038637804480950583 1.325964436977013 >1k 0.002458769375946976 2.0986034101612048 >5k 1.4050110719697007E-4 0.4346754242944075 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6992 0.23743394755331312 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5768 0.19586942355370568 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4090 0.13888799277646607 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.6978972222061865E-5 1.6978972222061865E-5 16-17 0.0 0.0 0.0 3.395794444412373E-5 3.395794444412373E-5 18-19 0.0 0.0 0.0 6.791588888824746E-5 3.395794444412373E-5 20-21 0.0 0.0 0.0 1.0187383333237119E-4 3.395794444412373E-5 22-23 0.0 0.0 0.0 1.8676869444268052E-4 3.395794444412373E-5 24-25 0.0 0.0 0.0 6.961378611045365E-4 3.395794444412373E-5 26-27 0.0 0.0 0.0 0.0014432126388752586 3.395794444412373E-5 28-29 0.0 0.0 0.0 0.004261722027737528 3.395794444412373E-5 30-31 0.0 0.0 0.0 0.013990673110978978 3.395794444412373E-5 32-33 0.0 0.0 0.0 0.03538417811077693 3.395794444412373E-5 34-35 0.0 0.0 0.0 0.06688017158270168 3.395794444412373E-5 36-37 0.0 0.0 0.0 0.11708699244333862 3.395794444412373E-5 38-39 0.0 0.0 0.0 0.1922019655537403 3.395794444412373E-5 40-41 0.0 0.0 0.0 0.275263097664067 3.395794444412373E-5 42-43 0.0 0.0 0.0 0.3613804447743647 3.395794444412373E-5 44-45 0.0 0.0 0.0 0.4727115656344244 3.395794444412373E-5 46-47 0.0 0.0 0.0 0.5966071259388098 3.395794444412373E-5 48-49 0.0 0.0 0.0 0.7292977938542233 3.395794444412373E-5 50-51 0.0 0.0 0.0 0.87494341757507 3.395794444412373E-5 52-53 0.0 0.0 0.0 1.0205211254070283 3.395794444412373E-5 54-55 0.0 0.0 0.0 1.179919716627745 3.395794444412373E-5 56-57 3.395794444412373E-5 0.0 0.0 1.367350590987086 3.395794444412373E-5 58-59 3.395794444412373E-5 0.0 0.0 1.571539710929602 3.395794444412373E-5 60-61 3.395794444412373E-5 0.0 0.0 1.7907382423164209 3.395794444412373E-5 62-63 3.395794444412373E-5 0.0 0.0 2.0046732923144 3.395794444412373E-5 64-65 3.395794444412373E-5 0.0 0.0 2.235604293506664 3.395794444412373E-5 66-67 3.395794444412373E-5 0.0 0.0 2.5025646737541427 3.395794444412373E-5 68-69 3.395794444412373E-5 0.0 0.0 2.799170339501341 3.395794444412373E-5 70-71 3.395794444412373E-5 0.0 0.0 3.109410119942855 3.395794444412373E-5 72-73 3.395794444412373E-5 0.0 0.0 3.4326388141342474 5.09369166661856E-5 74-75 3.395794444412373E-5 0.0 0.0 3.756393856464523 6.791588888824746E-5 76-77 3.395794444412373E-5 0.0 0.0 4.10790951837787 6.791588888824746E-5 78-79 3.395794444412373E-5 0.0 0.0 4.507645461401872 6.791588888824746E-5 80-81 3.395794444412373E-5 0.0 0.0 4.926329937425695 6.791588888824746E-5 82-83 3.395794444412373E-5 0.0 0.0 5.357341147282736 6.791588888824746E-5 84-85 3.395794444412373E-5 0.0 0.0 5.799796184417446 6.791588888824746E-5 86-87 3.395794444412373E-5 0.0 0.0 6.262761819996408 6.791588888824746E-5 88-89 3.395794444412373E-5 0.0 0.0 6.757817713075065 6.791588888824746E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10330 0.0 33.2131 1 GGTATCA 7110 0.0 32.749077 1 ATCAACG 12475 0.0 27.261715 3 TCAACGC 12565 0.0 27.10425 4 TATCAAC 12875 0.0 26.500237 2 CAACGCA 12850 0.0 26.46614 5 AACGCAG 13255 0.0 25.647024 6 GTACATG 9920 0.0 23.232859 1 TACATGG 9805 0.0 22.729916 2 ACGCAGA 14900 0.0 22.71993 7 CGCAGAG 14890 0.0 22.639526 8 ACATGGG 9890 0.0 21.41999 3 GAGTACT 8085 0.0 20.729956 12-13 GGACCGA 1200 0.0 20.574337 6 TATATCG 185 6.559507E-6 20.53993 5 GTACTTT 8300 0.0 20.192974 14-15 GCAGAGT 16250 0.0 20.101978 9 AGAGTAC 13000 0.0 19.996061 10-11 GTAAGGT 1545 0.0 19.983227 4 TAAGGTG 1695 0.0 19.335709 5 >>END_MODULE