FastQCFastQC Report
Thu 26 May 2016
SRR938478_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938478_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2974382
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70700.237696435763799No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53620.1802727423713565No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38870.13068260902600945No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA98350.033.7375031
GGTATCA66450.032.4532131
ATCAACG118900.027.500273
TCAACGC121400.027.0122534
TATCAAC121850.026.8734912
CAACGCA122200.026.8340595
AACGCAG126600.025.8202786
GTACATG98900.025.126311
TACATGG98200.024.63412
ACGCAGA141150.023.0886387
CGCAGAG141050.023.0043498
ACATGGG101650.022.4888953
TACACCG4050.022.2949775
TAAGGTG14800.021.1929155
GAGTACT75150.020.85807612-13
AGAGTAC123150.020.36640510-11
GCAGAGT154300.020.3512279
GTACTTT79100.019.66752414-15
AGTACTT81200.019.59648112-13
GGACCGA9450.018.6027056
Produced by FastQC (version 0.11.5)