##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938478_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2974382 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.781490407082885 31.0 31.0 34.0 27.0 34.0 2 30.881456383208345 31.0 31.0 34.0 27.0 34.0 3 30.94333545590311 31.0 31.0 34.0 27.0 34.0 4 34.56310823559314 37.0 35.0 37.0 30.0 37.0 5 34.570038414702616 37.0 35.0 37.0 30.0 37.0 6 34.5935885168751 37.0 35.0 37.0 30.0 37.0 7 34.50233023196079 37.0 35.0 37.0 30.0 37.0 8 34.45209458637122 37.0 35.0 37.0 30.0 37.0 9 35.918824818063044 39.0 35.0 39.0 30.0 39.0 10-11 35.75354258464448 38.0 35.0 39.0 30.0 39.0 12-13 35.59220705343161 38.0 35.0 39.0 30.0 39.0 14-15 36.52187832632123 39.0 36.0 41.0 29.5 41.0 16-17 36.35959284987604 39.0 36.0 40.5 29.0 41.0 18-19 36.35034807230544 39.0 35.5 40.5 29.0 41.0 20-21 36.42337601558912 39.0 36.0 41.0 29.0 41.0 22-23 36.33571662953851 39.0 36.0 41.0 29.0 41.0 24-25 36.21253927706663 39.0 35.0 41.0 28.0 41.0 26-27 36.00734606382099 39.0 35.0 40.5 27.0 41.0 28-29 35.81164641932341 39.0 35.0 40.0 27.0 41.0 30-31 35.59110985071857 38.0 34.0 40.0 27.0 41.0 32-33 35.30719238483826 38.0 34.0 40.0 26.0 41.0 34-35 35.04461582271544 38.0 34.0 40.0 25.0 41.0 36-37 34.74111311190022 38.0 33.5 40.0 24.5 41.0 38-39 34.46394814116008 38.0 33.0 40.0 24.0 41.0 40-41 34.39959443676031 38.0 33.0 40.0 24.0 41.0 42-43 34.21019475642335 38.0 33.0 40.0 23.5 41.0 44-45 33.90900395443491 38.0 33.0 40.0 22.5 41.0 46-47 33.54621615515425 38.0 32.5 40.0 19.5 41.0 48-49 33.18583557861768 37.5 31.5 40.0 18.0 41.0 50-51 32.45721733119687 36.5 30.5 39.5 15.0 40.5 52-53 32.53981381678614 37.0 31.0 39.0 15.0 40.0 54-55 32.656025520595534 37.0 31.0 40.0 14.0 41.0 56-57 32.33714196764235 37.0 31.0 40.0 11.0 41.0 58-59 31.986387592447777 36.0 31.0 39.0 8.0 41.0 60-61 31.512801146591123 36.0 30.0 39.0 7.5 40.5 62-63 30.976421488564682 35.0 29.0 39.0 3.5 40.0 64-65 30.457978329616033 35.0 28.5 38.5 2.0 40.0 66-67 29.898455544714835 34.5 28.0 38.0 2.0 40.0 68-69 29.31191269312415 34.0 27.5 37.5 2.0 40.0 70-71 28.668903489867812 34.0 26.0 37.0 2.0 39.0 72-73 28.08834104025643 33.5 26.0 36.0 2.0 39.0 74-75 27.45214165497236 33.0 25.0 36.0 2.0 38.5 76-77 26.686684830663985 32.5 23.0 35.0 2.0 37.5 78-79 25.88069286325697 32.0 20.5 35.0 2.0 37.0 80-81 25.20080238516774 31.0 18.5 35.0 2.0 36.0 82-83 24.520503755065757 31.0 15.0 34.0 2.0 36.0 84-85 23.840974696592433 30.5 7.5 34.0 2.0 35.5 86-87 23.14967949644666 30.0 4.0 34.0 2.0 35.0 88-89 22.33211117469108 29.0 2.0 34.0 2.0 35.0 90-91 21.478582945969954 29.0 2.0 34.0 2.0 35.0 92-93 20.610596587795378 28.0 2.0 34.0 2.0 35.0 94-95 19.631721648396205 26.5 2.0 33.5 2.0 35.0 96-97 18.390622320872033 25.0 2.0 33.0 2.0 34.5 98-99 16.923313313488315 17.5 2.0 32.0 2.0 34.0 100-101 13.701103624215047 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.749347521379665 1101 2 0.7955337079474312 1101 3 0.7884520391117462 1101 4 0.7349270428371355 1101 5 0.6706741894500325 1101 6 0.6908290153492374 1101 7 0.5968201948309897 1101 8 0.7215627734105041 1101 9 0.8424060301277976 1101 10-11 0.8105612779346032 1101 12-13 0.8259981624845025 1101 14-15 0.9961761240631759 1101 16-17 0.9571106384396515 1101 18-19 0.8859335315271792 1101 20-21 1.0590363209450544 1101 22-23 1.0677812209867383 1101 24-25 1.1055084600668934 1101 26-27 1.1620982044323185 1101 28-29 1.2384681723670568 1101 30-31 1.2448356443927011 1101 32-33 1.280509644818416 1101 34-35 1.2881828869112653 1101 36-37 1.2803111881256228 1101 38-39 1.3716274106669673 1101 40-41 1.3108061059642964 1101 42-43 1.4648762893870853 1101 44-45 1.427272717077237 1101 46-47 1.5551294905766397 1101 48-49 1.4624197607010458 1101 50-51 1.4717015191605114 1101 52-53 1.5529854677083037 1101 54-55 1.6082229055571418 1101 56-57 1.5234113873790704 1101 58-59 1.5230679752146017 1101 60-61 1.4773582570947958 1101 62-63 1.3941749343552274 1101 64-65 1.318578030502529 1101 66-67 1.201787705514068 1101 68-69 1.2067788957503147 1101 70-71 1.50590926383234 1101 72-73 1.5030757618349249 1101 74-75 1.5276170548356696 1101 76-77 1.729486754412072 1101 78-79 1.5849197689762988 1101 80-81 1.8081858728774165 1101 82-83 1.7444710110878 1101 84-85 1.898139579955906 1101 86-87 1.955616675789063 1101 88-89 2.199259878744904 1101 90-91 2.020343206220673 1101 92-93 1.944365848797986 1101 94-95 1.9485673786991775 1101 96-97 2.025678609170736 1101 98-99 2.1928710957364608 1101 100-101 1.7929444258859082 1102 1 0.7134825311190021 1102 2 0.7640306197452453 1102 3 0.7491848875989682 1102 4 0.7394531432844573 1102 5 0.742775145764945 1102 6 0.7387583048180275 1102 7 0.7534913967338284 1102 8 0.7612346702811905 1102 9 0.9339883544526515 1102 10-11 0.9453997223812323 1102 12-13 0.9252864322103349 1102 14-15 1.0404356651886246 1102 16-17 1.0470366635172255 1102 18-19 1.1628936344998735 1102 20-21 0.9234058823322542 1102 22-23 1.0796103567795399 1102 24-25 1.006058439022091 1102 26-27 0.9838083636876291 1102 28-29 1.0476778422083868 1102 30-31 1.026146392902433 1102 32-33 1.0945490964789926 1102 34-35 1.1547099074568692 1102 36-37 1.1512162613908998 1102 38-39 1.2865903864997108 1102 40-41 1.2156037088146476 1102 42-43 1.3481746704056548 1102 44-45 1.3251518416135184 1102 46-47 1.2020393307340314 1102 48-49 1.1102653603799908 1102 50-51 1.2650969999118544 1102 52-53 1.37762623785013 1102 54-55 1.4358667198538981 1102 56-57 1.3667771362313914 1102 58-59 1.3980373868411178 1102 60-61 1.486878949517692 1102 62-63 1.3530738263219888 1102 64-65 1.5492220748238559 1102 66-67 1.53026572593242 1102 68-69 1.3693061805794606 1102 70-71 1.3588417100212773 1102 72-73 1.493911607493331 1102 74-75 1.421194475232486 1102 76-77 1.3192506611101926 1102 78-79 1.3452054154432176 1102 80-81 1.6174920796254568 1102 82-83 1.6891508364648935 1102 84-85 1.3803979562760986 1102 86-87 1.2497297597822872 1102 88-89 1.4575720857082857 1102 90-91 1.6364233918064564 1102 92-93 1.5057410531283253 1102 94-95 1.7268178216188232 1102 96-97 1.9725831881278957 1102 98-99 2.100323402344525 1102 100-101 1.8357096637900412 1103 1 0.8408805834394641 1103 2 0.8149238684642199 1103 3 0.7674378235496988 1103 4 0.8818228575423248 1103 5 0.8418787108881389 1103 6 0.8237724925914307 1103 7 0.7933624135679906 1103 8 0.8059739488600854 1103 9 0.8405378767163967 1103 10-11 0.8360039303586149 1103 12-13 0.9186257224093382 1103 14-15 1.0819135832406488 1103 16-17 1.2081838583863913 1103 18-19 1.168873303064494 1103 20-21 0.9557671720139709 1103 22-23 1.1363295888030507 1103 24-25 1.2984551887662619 1103 26-27 1.3000902861777774 1103 28-29 1.134434811460089 1103 30-31 1.2060635715409305 1103 32-33 1.3520368016581799 1103 34-35 1.4012421494999074 1103 36-37 1.4509629618433308 1103 38-39 1.4630606577729282 1103 40-41 1.507285447257324 1103 42-43 1.3705309559453767 1103 44-45 1.3929500003891278 1103 46-47 1.5061801176775376 1103 48-49 1.410061258857759 1103 50-51 1.4353528577567474 1103 52-53 1.5250521034542501 1103 54-55 1.5436356971722063 1103 56-57 1.5477434041910172 1103 58-59 1.4562714494922275 1103 60-61 1.5075742160116796 1103 62-63 1.73233460959203 1103 64-65 1.590127421571541 1103 66-67 1.5473922613793647 1103 68-69 1.5377639677366197 1103 70-71 1.7696445187131111 1103 72-73 1.7995754247353233 1103 74-75 1.7188359687533428 1103 76-77 1.5842007696269782 1103 78-79 1.5627429183951023 1103 80-81 1.5480942821976207 1103 82-83 1.6910475374795908 1103 84-85 1.6980033056476778 1103 86-87 1.8922109895642443 1103 88-89 1.901022001181392 1103 90-91 1.897630701977043 1103 92-93 1.9375807031757404 1103 94-95 1.7837010470601058 1103 96-97 1.9551570132790488 1103 98-99 1.9476422505297286 1103 100-101 1.6991828007311067 1104 1 0.5719729278574839 1104 2 0.6088309620943839 1104 3 0.6560277290968379 1104 4 0.6251140519821519 1104 5 0.5730854686958935 1104 6 0.6478204338247551 1104 7 0.5147028709518082 1104 8 0.5297827234005226 1104 9 0.6028605809184526 1104 10-11 0.6404181742636865 1104 12-13 0.7262388208318313 1104 14-15 0.8137318570145808 1104 16-17 0.9234478183934058 1104 18-19 0.9589140558101903 1104 20-21 0.9563720569487231 1104 22-23 0.9966748969549499 1104 24-25 0.9270796655891189 1104 26-27 0.8897463267102381 1104 28-29 1.0127811221340295 1104 30-31 0.9839385170316035 1104 32-33 0.9608296617231886 1104 34-35 0.8971782263569352 1104 36-37 1.0000058455103158 1104 38-39 0.9660159623732909 1104 40-41 1.0233561452913946 1104 42-43 1.1026860636378117 1104 44-45 1.149717923280356 1104 46-47 1.3202721833674573 1104 48-49 1.2447777429275462 1104 50-51 1.1157942371911034 1104 52-53 1.2312146918492672 1104 54-55 1.2985789625168636 1104 56-57 1.3393940364991934 1104 58-59 1.3686064042438701 1104 60-61 1.423498918963947 1104 62-63 1.4828792532921113 1104 64-65 1.4335281966154838 1104 66-67 1.479104775821522 1104 68-69 1.5256881851625188 1104 70-71 1.6360238406845298 1104 72-73 1.539598307413268 1104 74-75 1.3176232767344054 1104 76-77 1.5899683058953507 1104 78-79 1.6492506800735853 1104 80-81 1.7354612682027728 1104 82-83 1.6153918521006787 1104 84-85 1.7071795292415608 1104 86-87 1.8491253570987105 1104 88-89 1.944992595366763 1104 90-91 1.9110702292182253 1104 92-93 2.033375392955861 1104 94-95 1.8413083011837088 1104 96-97 1.714851918399102 1104 98-99 1.6356824548104534 1104 100-101 1.3361056200208896 1105 1 0.6425273903288584 1105 2 0.6218307793163724 1105 3 0.5576802573467923 1105 4 0.6223422772076646 1105 5 0.632247485274938 1105 6 0.6557723524562888 1105 7 0.6001193360250809 1105 8 0.586203069818616 1105 9 0.6394785426210774 1105 10-11 0.7474293237223364 1105 12-13 0.7888682437909011 1105 14-15 0.959515060790558 1105 16-17 0.8926292954547606 1105 18-19 0.8145285662339106 1105 20-21 0.8974127089286341 1105 22-23 0.9269725025630109 1105 24-25 0.9851589912457754 1105 26-27 1.054937867313832 1105 28-29 0.975050902477868 1105 30-31 0.9602112433269099 1105 32-33 0.9334554291220414 1105 34-35 0.9568627730137109 1105 36-37 1.0389106826766081 1105 38-39 1.011340608375292 1105 40-41 1.1252823029750587 1105 42-43 1.1379434043252772 1105 44-45 1.1600228400088426 1105 46-47 1.1061375297317824 1105 48-49 1.1477188561531548 1105 50-51 0.9321958757422095 1105 52-53 1.0589405033298718 1105 54-55 1.160924541976918 1105 56-57 1.2940478872018204 1105 58-59 1.284355955362578 1105 60-61 1.28334474033608 1105 62-63 1.2766992061998934 1105 64-65 1.4355683217646629 1105 66-67 1.381324453487128 1105 68-69 1.2753844511152757 1105 70-71 1.34382308591886 1105 72-73 1.2674816305325365 1105 74-75 1.2808332886687346 1105 76-77 1.269944974819861 1105 78-79 1.4355407165216718 1105 80-81 1.3946039318159755 1105 82-83 1.1593085698376733 1105 84-85 1.191082162320079 1105 86-87 1.2875474452837246 1105 88-89 1.2153879334523268 1105 90-91 1.4084823076192379 1105 92-93 1.409070755770216 1105 94-95 1.3849734303647594 1105 96-97 1.4624586016233074 1105 98-99 1.4642737739301062 1105 100-101 0.8424732833652957 1106 1 0.29478861346386864 1106 2 0.44429045390450383 1106 3 0.2174486774587514 1106 4 0.4700102013505685 1106 5 0.4184254596530579 1106 6 0.006869931335792501 1106 7 0.038889179574610466 1106 8 0.27959717053160915 1106 9 0.2927553214393086 1106 10-11 0.11460153413577956 1106 12-13 0.16881277440757003 1106 14-15 0.5835175374442727 1106 16-17 0.293503145693812 1106 18-19 0.09308221323416177 1106 20-21 0.3497731897058429 1106 22-23 0.4093467708969527 1106 24-25 0.48572070651996313 1106 26-27 0.19891027943412354 1106 28-29 0.3020860032993724 1106 30-31 0.40463972995106445 1106 32-33 0.5746250096866987 1106 34-35 0.3740914741109691 1106 36-37 0.3864905196515167 1106 38-39 0.399652143326108 1106 40-41 0.621410297460038 1106 42-43 0.3001804992360064 1106 44-45 0.6909200524967929 1106 46-47 0.2999005752498718 1106 48-49 0.68755723534926 1106 50-51 0.3393880653466468 1106 52-53 0.4966548966454667 1106 54-55 0.24011714010155316 1106 56-57 -0.06196948767291133 1106 58-59 -0.40043815684004613 1106 60-61 0.3962699364576885 1106 62-63 0.5494017588128237 1106 64-65 0.5892552165643288 1106 66-67 0.17316904985002068 1106 68-69 -0.11940563971663565 1106 70-71 -0.09108336933860173 1106 72-73 0.47952723159979627 1106 74-75 0.5326224794795813 1106 76-77 0.6947517256161575 1106 78-79 0.7697485384068621 1106 80-81 0.682897274851662 1106 82-83 0.7570358570720543 1106 84-85 0.6199683702951582 1106 86-87 0.8520805513776999 1106 88-89 0.5581446788229627 1106 90-91 0.5311561361556514 1106 92-93 0.45344753905662927 1106 94-95 0.3906738829285388 1106 96-97 0.48843569895963057 1106 98-99 0.5449654496927288 1106 100-101 0.11472837869731478 1107 1 0.47403777499150834 1107 2 0.3557273833063803 1107 3 0.35640726566271397 1107 4 0.41678842853288955 1107 5 0.4604335489347591 1107 6 0.39705189856255174 1107 7 0.39128725212847826 1107 8 0.43496797088780426 1107 9 0.4017230225686461 1107 10-11 0.5857491385599118 1107 12-13 0.5872306623726828 1107 14-15 0.5764910142541666 1107 16-17 0.554759585078294 1107 18-19 0.5707112297872285 1107 20-21 0.6305704042299283 1107 22-23 0.7321889009080209 1107 24-25 0.7370075431281222 1107 26-27 0.6406809749292606 1107 28-29 0.6064798689274156 1107 30-31 0.7878048486687632 1107 32-33 0.6853828388963237 1107 34-35 0.7689495371774058 1107 36-37 0.7245406240964627 1107 38-39 0.8644652243271196 1107 40-41 0.7929280364558693 1107 42-43 0.8308774299694548 1107 44-45 0.9293117876144308 1107 46-47 0.8590521993479356 1107 48-49 0.885989000760091 1107 50-51 0.9300037287592957 1107 52-53 0.8756209957308485 1107 54-55 0.8963921590348036 1107 56-57 0.8496078806473975 1107 58-59 0.8974233225331147 1107 60-61 0.7899488327534279 1107 62-63 0.7906939216969988 1107 64-65 0.7654134735405016 1107 66-67 0.9290012180604741 1107 68-69 0.9209975968282755 1107 70-71 0.9024595612783308 1107 72-73 0.8589967261861347 1107 74-75 0.9038884687105195 1107 76-77 0.9215513203198391 1107 78-79 0.8455735910454507 1107 80-81 0.7209542451992839 1107 82-83 0.9312162139325473 1107 84-85 0.8415896179753162 1107 86-87 0.7523240828651474 1107 88-89 0.6815758200608215 1107 90-91 0.9123775802422358 1107 92-93 1.1688967563437274 1107 94-95 0.9588074601054473 1107 96-97 1.1099100579339911 1107 98-99 0.9973797507438391 1107 100-101 0.6822939584707814 1108 1 0.3707068721016711 1108 2 0.43002743550114886 1108 3 0.4382190816024405 1108 4 0.30707691420721517 1108 5 0.35200967162035823 1108 6 0.3813561143859374 1108 7 0.3361635983351974 1108 8 0.24866292233869558 1108 9 0.3537968682075956 1108 10-11 0.354483973190149 1108 12-13 0.4487193887007592 1108 14-15 0.5334596137210568 1108 16-17 0.44595615018429413 1108 18-19 0.4079353644895818 1108 20-21 0.4884609286877648 1108 22-23 0.46875537154546265 1108 24-25 0.39431949254302623 1108 26-27 0.5490270581301075 1108 28-29 0.38075237941617246 1108 30-31 0.5260439425516168 1108 32-33 0.37927306253697424 1108 34-35 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82-83 0.05054397331531746 2311 84-85 0.3657065917241482 2311 86-87 0.4838355845840674 2311 88-89 0.34004506959742997 2311 90-91 0.34639023054879203 2311 92-93 0.4224639130744485 2311 94-95 0.41968399089551767 2311 96-97 0.1415277049928143 2311 98-99 0.2860875500499347 2311 100-101 0.37655755537197066 2312 1 -0.46417215344825635 2312 2 -0.3265043250215278 2312 3 -0.2981157713646745 2312 4 -0.28784690240852484 2312 5 -0.19509470610859836 2312 6 -0.09986988645676576 2312 7 -0.16275828907292578 2312 8 -0.32155514113376427 2312 9 -0.323112088916119 2312 10-11 -0.3025702221739195 2312 12-13 -0.25777502139660413 2312 14-15 -0.33845433867238484 2312 16-17 -0.3351122044801471 2312 18-19 -0.5113707862141652 2312 20-21 -0.39381824520386033 2312 22-23 -0.5281631079491902 2312 24-25 -0.41900847010242614 2312 26-27 -0.4405232144823543 2312 28-29 -0.38657581819686726 2312 30-31 -0.3014645205512565 2312 32-33 -0.36373775568909394 2312 34-35 -0.5102439201726199 2312 36-37 -0.45686373069705155 2312 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-1.2679866675068183 2315 98-99 -1.162732881976245 2315 100-101 -0.9408180684399099 2316 1 -0.8902603150633226 2316 2 -0.8862129334550701 2316 3 -0.8341087456705765 2316 4 -0.8928555715486439 2316 5 -0.8637220596182047 2316 6 -0.9396421840726461 2316 7 -0.7465732831453735 2316 8 -0.8367946007096592 2316 9 -1.0159832087039078 2316 10-11 -1.1986191730648628 2316 12-13 -0.9896232278411432 2316 14-15 -1.6165734591350187 2316 16-17 -1.4314908990326742 2316 18-19 -1.3355378784294132 2316 20-21 -1.2990024060597136 2316 22-23 -1.1479653521342428 2316 24-25 -1.2406168479112338 2316 26-27 -1.4117553772181566 2316 28-29 -1.650335649122276 2316 30-31 -1.2954803283629772 2316 32-33 -1.3828829227376218 2316 34-35 -1.36215994324413 2316 36-37 -1.4358012601653485 2316 38-39 -1.540002355525715 2316 40-41 -1.5055370769789747 2316 42-43 -1.5818055130175637 2316 44-45 -1.3985610287903043 2316 46-47 -1.4802178632297753 2316 48-49 -1.525991726527458 2316 50-51 -1.6596900583134158 2316 52-53 -1.3975437141083304 2316 54-55 -1.3784075719014197 2316 56-57 -1.4967225582119354 2316 58-59 -1.5305651709135475 2316 60-61 -1.6844006749821538 2316 62-63 -1.5444724086399404 2316 64-65 -1.5468003913976318 2316 66-67 -1.5888739424605305 2316 68-69 -1.4373619804154494 2316 70-71 -1.8571344989348795 2316 72-73 -1.555107209890835 2316 74-75 -1.5023835710783082 2316 76-77 -1.3729759325797275 2316 78-79 -1.5614661468212034 2316 80-81 -1.5354673593992594 2316 82-83 -1.3247681620229343 2316 84-85 -1.342198015282598 2316 86-87 -1.3856620664652546 2316 88-89 -1.5307137190655347 2316 90-91 -1.5263479848936221 2316 92-93 -1.7361178689034276 2316 94-95 -1.8590121550518113 2316 96-97 -2.0629764154959638 2316 98-99 -1.9161070712460102 2316 100-101 -1.4573656860388908 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13281.0 3 4976.0 4 5150.0 5 5707.0 6 6573.0 7 8003.0 8 9834.0 9 12356.0 10 15318.0 11 18675.0 12 22072.0 13 25052.0 14 27872.0 15 31888.0 16 35276.0 17 40198.0 18 38648.0 19 37902.0 20 40907.0 21 44356.0 22 48679.0 23 53637.0 24 59394.0 25 66598.0 26 74153.0 27 83900.0 28 94470.0 29 106654.0 30 122310.0 31 141841.0 32 165048.0 33 195097.0 34 235952.0 35 283834.0 36 328085.0 37 316756.0 38 146347.0 39 7583.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.2386934884935 18.526764287338224 14.395102686954253 23.839439537214023 2 15.425394680056282 19.69035784168127 40.29966520353695 24.58458227472551 3 19.499331976206783 23.61316107782578 28.976793405064605 27.910713540902833 4 12.96281781695748 16.13867471131861 36.63474214538031 34.263765326343595 5 13.97905494963903 37.254401959267156 34.0275656509248 14.738977440169013 6 33.030583181773224 35.598963537429796 17.150986166817724 14.21946711397926 7 29.42079036090116 31.894771466932863 20.26944488541731 18.41499328674866 8 26.40548181461678 35.062801808885816 19.533034094524577 18.998682281972822 9 26.54326448144813 16.78054903691336 19.734337628405665 36.94184885323284 10-11 25.093610724354026 26.31727295920212 27.27225791001161 21.316858406432246 12-13 26.138684593293355 23.535474839619106 27.4028629169033 22.92297765018424 14-15 22.803806525712883 25.084795926432278 25.61824091964693 26.49315662820791 16-17 22.579972529726952 28.261724977096897 26.415704467215267 22.742598025960888 18-19 22.649069213454045 26.987991722604672 28.334220437363307 22.028718626577973 20-21 23.03648049901846 26.154521749309424 28.60443893388086 22.204558817791256 22-23 22.7414764557071 26.091643678999898 28.418020222867966 22.748859642425035 24-25 22.68783195524351 26.256068008118827 28.45654836861461 22.599551668023057 26-27 22.237585484895572 26.641080415046925 28.39083288076088 22.730501219296624 28-29 22.32425951672723 27.06299256165782 28.25867045582781 22.354077465787142 30-31 22.43426477956565 26.57513837643807 28.130652855569295 22.85994398842699 32-33 22.175152431614954 26.48699977345281 28.220935147123647 23.116912647808586 34-35 22.922245855961933 26.582188427488102 28.0666066694462 22.42895904710376 36-37 22.91842206437798 26.201902943619064 28.013624745330684 22.86605024667227 38-39 22.42266784460021 26.61180890707429 27.96841102392445 22.997112224401047 40-41 22.882689834345314 26.407713412465693 28.02417453038387 22.685422222805123 42-43 22.546060519749062 26.472047586132337 27.84139144073079 23.140500453387816 44-45 22.54889451386567 26.683790400521545 28.01872212833252 22.748592957280266 46-47 22.928147025708213 26.93223510811411 27.398675962979958 22.74094190319772 48-49 22.666227531299324 26.839308062538798 27.451609367234852 23.04285503892703 50-51 22.847418418528644 27.01481709997739 27.35545825246146 22.782306229032503 52-53 22.98940296101104 26.86699827560438 27.343178264377404 22.800420499007174 54-55 22.553917701017788 26.975317971659152 27.51283478587171 22.957929541451353 56-57 22.634517019783214 27.061064435188648 27.44358190888879 22.860836636139354 58-59 22.69092043753087 26.605258884088478 27.41407096645554 23.289749711925108 60-61 22.43822397067971 27.00201745095072 27.517459356769386 23.042299221600175 62-63 22.42224904513422 26.94503469793964 27.740616090160845 22.892100166765292 64-65 22.240737257030098 26.9805133181324 27.552674965818074 23.22607445901943 66-67 22.29438422943069 26.796159299372718 27.49877364000122 23.41068283119537 68-69 22.63029223414507 26.554391514537734 27.664702389478308 23.150613861838888 70-71 22.52160486613701 26.62345665415753 27.380679952389176 23.474258527316284 72-73 22.541535079157633 26.659793263223946 27.349353003911013 23.44931865370741 74-75 22.355749668600698 27.006504236732425 27.36038354214908 23.277362552517793 76-77 22.60965293138873 26.547049209845902 27.442019752129156 23.401278106636212 78-79 22.569463532181462 26.64122456946051 27.469933557023403 23.31937834133463 80-81 22.619523543262424 26.781971936306114 27.22073403508819 23.377770485343273 82-83 22.56465544052513 26.651265269288537 27.365661698029275 23.418417592157056 84-85 22.507662091630394 26.683092258963313 27.362732521008446 23.446513128397843 86-87 22.585335592810022 26.76925094312978 27.121453596669966 23.523959867390236 88-89 22.571181386104318 26.688935766287113 27.052617684176187 23.68726516343238 90-91 22.561962652058348 26.585057661850552 27.14613778775852 23.706841898332584 92-93 22.623958344017307 26.27869309196771 27.338739522488975 23.75860904152601 94-95 22.4365163844145 26.120844620897593 27.473267180219313 23.969371814468598 96-97 22.60477818454548 25.908744646109295 27.413316158281773 24.073161011063444 98-99 22.48824117108419 26.055015884129084 27.071637136343902 24.385105808442816 100-101 22.98999888913949 25.438638421635524 27.320924235946777 24.250438453278218 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 585.0 1 401.0 2 300.0 3 395.5 4 355.0 5 372.5 6 633.5 7 1228.5 8 1926.0 9 2115.0 10 1802.0 11 1541.0 12 1532.5 13 1669.5 14 1841.0 15 2000.0 16 2179.0 17 2331.0 18 2582.0 19 3003.5 20 3409.5 21 3973.5 22 4866.0 23 5891.0 24 7170.0 25 9255.5 26 12042.0 27 15519.0 28 19366.0 29 23683.5 30 28726.5 31 35498.0 32 44688.5 33 50508.0 34 53729.5 35 59398.0 36 66781.0 37 73282.0 38 77700.5 39 82053.5 40 87601.0 41 94142.0 42 98635.5 43 102511.5 44 106647.0 45 111014.5 46 113531.5 47 115612.5 48 118297.5 49 116223.0 50 112313.5 51 108345.5 52 104476.5 53 102609.5 54 101579.0 55 96373.0 56 88640.0 57 81566.0 58 75427.5 59 67133.5 60 55622.5 61 46500.5 62 38788.5 63 30030.5 64 22528.0 65 17690.5 66 13533.0 67 10051.0 68 7762.5 69 6036.0 70 4593.5 71 3330.0 72 2480.0 73 1937.5 74 1485.5 75 1099.5 76 769.5 77 508.5 78 342.0 79 215.0 80 123.5 81 76.5 82 39.5 83 32.5 84 25.0 85 15.0 86 14.0 87 6.0 88 4.0 89 6.0 90 3.5 91 2.5 92 3.0 93 1.5 94 1.0 95 1.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18178566169375687 2 0.051472877391000885 3 0.04821169574049332 4 0.05517112462353524 5 0.06192883093025711 6 0.06603052331543158 7 0.038932457229770755 8 0.03563765514987651 9 0.0432022517618786 10-11 0.043656127558598726 12-13 0.04120183621337138 14-15 0.035872998155583244 16-17 0.03314974337526249 18-19 0.041168215783984705 20-21 0.050632366656333985 22-23 0.04743842586459977 24-25 0.06828309208433887 26-27 0.05249830048729451 28-29 0.04506818559283912 30-31 0.05391035852153489 32-33 0.05014487043022718 34-35 0.04930435969556029 36-37 0.04737118500582642 38-39 0.027333409091367555 40-41 0.025433854831020362 42-43 0.02213905275112612 44-45 0.004959013334534703 46-47 0.012489989517150118 48-49 0.01196887286165664 50-51 0.00956501216050931 52-53 0.009968457313149421 54-55 0.011279654059229783 56-57 0.00906070571970917 58-59 0.011061121268216388 60-61 0.011161982556376416 62-63 0.004774100972907986 64-65 0.0074805455385354 66-67 0.0044715171084279024 68-69 0.008741311640535748 70-71 0.023147665632726397 72-73 0.02018906784669891 74-75 0.0211136296548325 76-77 0.012557230375923469 78-79 0.0059676262161349825 80-81 0.007362874035682035 82-83 0.006488742871628459 84-85 0.0054296993459481665 86-87 0.00813614391157558 88-89 0.0304096783802484 90-91 0.036948851895956876 92-93 0.023248526920886425 94-95 0.03284715951078241 96-97 0.03620920244945 98-99 0.022155862965819454 100-101 0.12479903388334114 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2974382.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.95728877185872 #Duplication Level Percentage of deduplicated Percentage of total 1 73.72791607541586 38.30702626077747 2 13.904224071146812 14.44851570426408 3 5.1397842989868225 8.011477711425714 4 2.408838662203408 5.0062690390768125 5 1.3291073546284207 3.452840731661505 6 0.8074911234594867 2.5173029689378303 7 0.5210887678514696 1.8952051710921605 8 0.3627787946463006 1.5079202074995741 9 0.27152394817026815 1.269688336520204 >10 1.3892736553339806 12.787932899302504 >50 0.09048540198325367 3.2419800247875585 >100 0.04193993965594662 4.058369008956499 >500 0.003132935445657682 1.1011309715725444 >1k 0.0022844320953408536 1.9727437902259508 >5k 1.305389768766202E-4 0.42159717389962564 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7070 0.237696435763799 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5362 0.1802727423713565 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3887 0.13068260902600945 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.3620429386675955E-5 0.0 5 0.0 0.0 0.0 3.3620429386675955E-5 0.0 6 0.0 0.0 0.0 3.3620429386675955E-5 0.0 7 0.0 0.0 0.0 3.3620429386675955E-5 0.0 8 0.0 0.0 0.0 3.3620429386675955E-5 0.0 9 0.0 0.0 0.0 6.724085877335191E-5 0.0 10-11 0.0 0.0 0.0 6.724085877335191E-5 0.0 12-13 0.0 0.0 0.0 8.40510734666899E-5 0.0 14-15 0.0 0.0 0.0 1.0086128816002787E-4 0.0 16-17 0.0 0.0 0.0 1.0086128816002787E-4 0.0 18-19 0.0 0.0 0.0 1.0086128816002787E-4 3.3620429386675955E-5 20-21 0.0 0.0 0.0 1.3448171754670382E-4 3.3620429386675955E-5 22-23 0.0 0.0 0.0 3.866349379467735E-4 3.3620429386675955E-5 24-25 0.0 0.0 0.0 8.573209493602369E-4 3.3620429386675955E-5 26-27 0.0 0.0 0.0 0.0015297295370937558 3.3620429386675955E-5 28-29 0.0 0.0 0.0 0.004252984317414508 3.3620429386675955E-5 30-31 0.0 0.0 0.0 0.014322302918723957 3.3620429386675955E-5 32-33 0.0 0.0 0.0 0.03578894708211655 3.3620429386675955E-5 34-35 0.0 0.0 0.0 0.06505553086321797 3.3620429386675955E-5 36-37 0.0 0.0 0.0 0.11607453245749873 3.3620429386675955E-5 38-39 0.0 0.0 0.0 0.19363686305256017 3.3620429386675955E-5 40-41 0.0 0.0 0.0 0.2768810462139698 3.3620429386675955E-5 42-43 0.0 0.0 0.0 0.36565579000948767 3.3620429386675955E-5 44-45 0.0 0.0 0.0 0.47487175487210453 3.3620429386675955E-5 46-47 0.0 0.0 0.0 0.6028815397618732 3.3620429386675955E-5 48-49 0.0 0.0 0.0 0.7400192712301245 3.3620429386675955E-5 50-51 0.0 0.0 0.0 0.884587117592831 3.3620429386675955E-5 52-53 0.0 0.0 0.0 1.0303821096281514 5.0430644080013936E-5 54-55 0.0 0.0 0.0 1.1893899304124353 6.724085877335191E-5 56-57 0.0 0.0 0.0 1.3795638892381676 8.40510734666899E-5 58-59 0.0 0.0 0.0 1.5831355891744907 1.0086128816002787E-4 60-61 0.0 0.0 0.0 1.8056187806408188 1.0086128816002787E-4 62-63 0.0 0.0 0.0 2.0283541253275468 1.0086128816002787E-4 64-65 0.0 0.0 0.0 2.2534260898566494 1.0086128816002787E-4 66-67 0.0 0.0 0.0 2.515228373490695 1.0086128816002787E-4 68-69 3.3620429386675955E-5 0.0 0.0 2.8077597295841623 1.0086128816002787E-4 70-71 6.724085877335191E-5 0.0 0.0 3.1174879353089144 1.0086128816002787E-4 72-73 6.724085877335191E-5 0.0 0.0 3.435587627950949 1.0086128816002787E-4 74-75 6.724085877335191E-5 0.0 0.0 3.761655362357626 1.0086128816002787E-4 76-77 6.724085877335191E-5 0.0 0.0 4.1162164106695105 1.0086128816002787E-4 78-79 6.724085877335191E-5 0.0 0.0 4.505944428119858 1.0086128816002787E-4 80-81 6.724085877335191E-5 0.0 0.0 4.922652840153013 1.0086128816002787E-4 82-83 6.724085877335191E-5 0.0 0.0 5.3500525487311315 1.0086128816002787E-4 84-85 6.724085877335191E-5 0.0 0.0 5.788126743639519 1.0086128816002787E-4 86-87 6.724085877335191E-5 0.0 0.0 6.235984483499429 1.0086128816002787E-4 88-89 6.724085877335191E-5 0.0 0.0 6.714957930756709 1.0086128816002787E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9835 0.0 33.737503 1 GGTATCA 6645 0.0 32.453213 1 ATCAACG 11890 0.0 27.50027 3 TCAACGC 12140 0.0 27.012253 4 TATCAAC 12185 0.0 26.873491 2 CAACGCA 12220 0.0 26.834059 5 AACGCAG 12660 0.0 25.820278 6 GTACATG 9890 0.0 25.12631 1 TACATGG 9820 0.0 24.6341 2 ACGCAGA 14115 0.0 23.088638 7 CGCAGAG 14105 0.0 23.004349 8 ACATGGG 10165 0.0 22.488895 3 TACACCG 405 0.0 22.294977 5 TAAGGTG 1480 0.0 21.192915 5 GAGTACT 7515 0.0 20.858076 12-13 AGAGTAC 12315 0.0 20.366405 10-11 GCAGAGT 15430 0.0 20.351227 9 GTACTTT 7910 0.0 19.667524 14-15 AGTACTT 8120 0.0 19.596481 12-13 GGACCGA 945 0.0 18.602705 6 >>END_MODULE