FastQCFastQC Report
Thu 26 May 2016
SRR938478_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938478_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2974382
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72220.24280674103057376No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58570.19691485491776106No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43090.14487043022718668No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA29910.10055870429554778No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA29810.10022250000168101No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67700.035.476241
GTATCAA100400.033.6798971
ATCAACG125700.026.511983
TATCAAC127850.026.076672
TCAACGC128250.025.9848444
CAACGCA130850.025.4685235
AACGCAG136500.024.5653236
TAAGGTG17350.023.564515
GTACATG101250.023.0163211
TACATGG102300.022.0827352
ACGCAGA154050.021.735917
CGCAGAG154850.021.5622738
GTAAGGT17800.021.0992264
ACATGGG101450.021.0872483
GAGTACT87600.020.46742812-13
AGTACTT90750.020.25418312-13
AGAGTAC139850.019.88450810-11
GTACTTT89700.019.64408914-15
GCAGAGT168100.019.5236389
AGGTAAG17600.019.456052
Produced by FastQC (version 0.11.5)