##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938478_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2974382 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.774194773905975 31.0 31.0 34.0 30.0 34.0 2 32.00571580920003 31.0 31.0 34.0 30.0 34.0 3 32.074156581098194 33.0 31.0 34.0 30.0 34.0 4 35.745734071817274 37.0 35.0 37.0 33.0 37.0 5 35.60864441756304 37.0 35.0 37.0 33.0 37.0 6 35.636484150320975 37.0 35.0 37.0 33.0 37.0 7 35.572606343099174 37.0 35.0 37.0 33.0 37.0 8 35.56103788955151 37.0 35.0 37.0 33.0 37.0 9 37.135465115106264 39.0 37.0 39.0 33.0 39.0 10-11 37.04286537505942 39.0 37.0 39.0 33.0 39.0 12-13 36.940728023502025 39.0 37.0 39.0 33.0 39.0 14-15 38.000024206709156 40.0 37.0 41.0 33.0 41.0 16-17 37.92684076893956 40.0 37.0 41.0 33.0 41.0 18-19 38.025837299983664 40.0 37.0 41.0 33.0 41.0 20-21 38.06791309925894 40.0 37.0 41.0 33.0 41.0 22-23 37.976981604918265 40.0 37.0 41.0 33.0 41.0 24-25 37.88934239112528 40.0 37.0 41.0 32.0 41.0 26-27 37.704228979330836 40.0 37.0 41.0 32.0 41.0 28-29 37.58726216067741 39.5 37.0 41.0 32.0 41.0 30-31 37.358857402983205 39.0 36.0 41.0 31.5 41.0 32-33 37.159081449524635 39.0 36.0 41.0 31.0 41.0 34-35 37.01833355634885 39.0 36.0 41.0 31.0 41.0 36-37 37.027570096914246 39.0 36.0 41.0 31.0 41.0 38-39 36.91174637285998 39.0 36.0 41.0 30.0 41.0 40-41 36.74815978579752 39.0 35.0 41.0 30.0 41.0 42-43 36.54062911219877 39.0 35.0 40.5 30.0 41.0 44-45 36.2944509145093 39.0 35.0 40.0 30.0 41.0 46-47 35.9679267827737 38.5 35.0 40.0 29.0 41.0 48-49 35.643862825958465 38.0 35.0 40.0 28.0 41.0 50-51 35.29108366040408 38.0 34.0 40.0 27.0 41.0 52-53 34.80960649977037 38.0 33.5 40.0 26.0 41.0 54-55 34.51020077448021 38.0 33.0 40.0 25.5 41.0 56-57 34.18566394632566 37.0 33.0 40.0 25.0 41.0 58-59 33.758941857501824 37.0 32.5 40.0 23.0 41.0 60-61 33.39518696656987 36.5 32.0 39.0 23.0 40.5 62-63 32.94252251392054 36.0 31.5 39.0 22.0 40.0 64-65 32.447078922613166 35.0 31.0 39.0 20.5 40.0 66-67 31.968145651769007 35.0 30.5 38.0 19.5 40.0 68-69 31.431539223946352 35.0 30.0 38.0 18.0 40.0 70-71 30.863102654601864 34.0 29.0 37.0 14.5 39.0 72-73 30.41891004585154 34.0 29.0 36.5 12.5 39.0 74-75 29.902094451889504 34.0 29.0 36.0 8.0 39.0 76-77 28.63383553289389 32.5 27.0 34.5 7.5 37.0 78-79 29.331081549041112 33.5 29.0 35.0 7.0 37.0 80-81 29.065763913310395 34.0 28.5 35.0 4.5 37.0 82-83 28.59798153028091 33.0 27.5 35.0 2.0 36.0 84-85 28.075755904924115 33.0 26.5 35.0 2.0 36.0 86-87 27.51204653605354 32.5 26.0 35.0 2.0 35.5 88-89 26.879219784143395 32.0 25.0 34.5 2.0 35.0 90-91 26.34719077778174 32.0 24.5 34.0 2.0 35.0 92-93 25.612616839397226 31.5 22.5 34.0 2.0 35.0 94-95 24.8532609463075 31.0 19.0 34.0 2.0 35.0 96-97 23.865589725865743 31.0 8.5 34.0 2.0 35.0 98-99 22.637641365500464 30.5 2.0 34.0 2.0 35.0 100-101 18.96772287486947 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2986846195778199 1101 2 0.25103639681385914 1101 3 0.3040089049886632 1101 4 0.22222088571981402 1101 5 0.1894876921568951 1101 6 0.19062039294846045 1101 7 0.3328412643968335 1101 8 0.27692852936175427 1101 9 0.2729926472105859 1101 10-11 0.32605414413806244 1101 12-13 0.35118574527667334 1101 14-15 0.5188173932021911 1101 16-17 0.4172385764551052 1101 18-19 0.5086782164830694 1101 20-21 0.4404021860108216 1101 22-23 0.4021186888536832 1101 24-25 0.45368420791793085 1101 26-27 0.5058641603409484 1101 28-29 0.45164642687238654 1101 30-31 0.3583032543746043 1101 32-33 0.3378526117708347 1101 34-35 0.16197527651437582 1101 36-37 0.3785652241137143 1101 38-39 0.5812317366699773 1101 40-41 0.6136302388866568 1101 42-43 0.6474314327041668 1101 44-45 0.6968956225169407 1101 46-47 0.6167489319238371 1101 48-49 0.5836392626536409 1101 50-51 0.6352016543307499 1101 52-53 0.6568681825562095 1101 54-55 0.6236540945018447 1101 56-57 0.7745050149162793 1101 58-59 0.7370037550882103 1101 60-61 0.5255034503594658 1101 62-63 0.46285851775363795 1101 64-65 0.550119783379138 1101 66-67 0.32340209163271894 1101 68-69 0.2762657286379877 1101 70-71 0.26673512670694777 1101 72-73 0.2166845494750156 1101 74-75 0.3751990094233584 1101 76-77 0.24442881444327114 1101 78-79 0.26936902279750896 1101 80-81 0.46654700136364724 1101 82-83 0.3696600053870256 1101 84-85 0.39158668732915203 1101 86-87 0.45426923977347045 1101 88-89 0.2781355414208413 1101 90-91 0.4651881062475276 1101 92-93 0.5409821467240157 1101 94-95 0.5050180999611413 1101 96-97 0.6234113024174377 1101 98-99 0.4913043726184725 1101 100-101 0.44280896413097537 1102 1 0.38143497929709014 1102 2 0.3573092157404716 1102 3 0.3016450354860041 1102 4 0.2744381757367478 1102 5 0.23829254322822635 1102 6 0.25790894164025957 1102 7 0.2739077463912949 1102 8 0.28853155589777657 1102 9 0.30801633484701085 1102 10-11 0.412680662544922 1102 12-13 0.4101641670144929 1102 14-15 0.46353490345541104 1102 16-17 0.4039739894715524 1102 18-19 0.4446454942646554 1102 20-21 0.41485343059056845 1102 22-23 0.40138750437397874 1102 24-25 0.5154098326548748 1102 26-27 0.5150160793331438 1102 28-29 0.3868190187109164 1102 30-31 0.430092086926507 1102 32-33 0.3671549276777668 1102 34-35 0.3791261775255137 1102 36-37 0.3894393908864231 1102 38-39 0.46952874732941297 1102 40-41 0.4928219472350719 1102 42-43 0.58314923171163 1102 44-45 0.5175342098724371 1102 46-47 0.4246132027459524 1102 48-49 0.6212738514969516 1102 50-51 0.65110858736913 1102 52-53 0.48867372859537994 1102 54-55 0.4665288387830344 1102 56-57 0.23936680440954206 1102 58-59 0.4439021674292718 1102 60-61 0.572587277185221 1102 62-63 0.5912291118043242 1102 64-65 0.6789421065293979 1102 66-67 0.6074312900705117 1102 68-69 0.38270543773032273 1102 70-71 0.3086755797085239 1102 72-73 0.17322214136248704 1102 74-75 0.1915234125830203 1102 76-77 0.10751158413484418 1102 78-79 0.11066442817910627 1102 80-81 0.2438773460089685 1102 82-83 0.22460467713707288 1102 84-85 0.22170277632418944 1102 86-87 0.03235952196957115 1102 88-89 0.24383043585424602 1102 90-91 0.21395612891718585 1102 92-93 0.22599396607152755 1102 94-95 0.2143356855841141 1102 96-97 0.22759383425381685 1102 98-99 0.1678988833513202 1102 100-101 0.1351121952420442 1103 1 0.3302385770315439 1103 2 0.30321200419083993 1103 3 0.24872576522859902 1103 4 0.26075878193138635 1103 5 0.26592777688604485 1103 6 0.23576233339898067 1103 7 0.3137614267784343 1103 8 0.21930290596656477 1103 9 0.4260227184079355 1103 10-11 0.42543325926355635 1103 12-13 0.3771909476081632 1103 14-15 0.5550404192568763 1103 16-17 0.5393524300534622 1103 18-19 0.46362234401669156 1103 20-21 0.4875833399912395 1103 22-23 0.5711141320320792 1103 24-25 0.6238886671958284 1103 26-27 0.6113671320771772 1103 28-29 0.5961863695893683 1103 30-31 0.652130506367314 1103 32-33 0.7437585490272767 1103 34-35 0.6577676279395206 1103 36-37 0.6089148274837584 1103 38-39 0.4726251773332777 1103 40-41 0.47716502708769326 1103 42-43 0.538287426622027 1103 44-45 0.6719232359951874 1103 46-47 0.68572094548432 1103 48-49 0.6577537187278892 1103 50-51 0.6733376608719155 1103 52-53 0.8026386583687142 1103 54-55 0.7292371572788738 1103 56-57 0.8668515389651077 1103 58-59 0.797435194766507 1103 60-61 0.6955739065675957 1103 62-63 0.6762498593144315 1103 64-65 0.716222027362754 1103 66-67 0.6856041410070581 1103 68-69 0.6009305416552877 1103 70-71 0.3848212377250846 1103 72-73 0.5298614383086466 1103 74-75 0.5476749462494617 1103 76-77 0.49628047149104404 1103 78-79 0.41987706298169414 1103 80-81 0.6565007159143974 1103 82-83 0.5646935240852962 1103 84-85 0.4306847426708664 1103 86-87 0.5399343779364933 1103 88-89 0.44377050797386985 1103 90-91 0.5014480698120813 1103 92-93 0.377204096928903 1103 94-95 0.43320715785142383 1103 96-97 0.5242312195052783 1103 98-99 0.6208866560020923 1103 100-101 0.31755721527735403 1104 1 0.2809216856590808 1104 2 0.28000227028626057 1104 3 0.22967717213116856 1104 4 0.19052559440581973 1104 5 0.09815256660367311 1104 6 0.1527654218861585 1104 7 0.16141269296826266 1104 8 0.17211536241529757 1104 9 0.2809930698301244 1104 10-11 0.28049869293301555 1104 12-13 0.2576031055480925 1104 14-15 0.46349003915266707 1104 16-17 0.41907462355369773 1104 18-19 0.35785469535796466 1104 20-21 0.4195277157526789 1104 22-23 0.38797560866162684 1104 24-25 0.39828524333580617 1104 26-27 0.4588858900288031 1104 28-29 0.47711394973493526 1104 30-31 0.4791401584517132 1104 32-33 0.48816488065077124 1104 34-35 0.5124319404027133 1104 36-37 0.5805174915849278 1104 38-39 0.5228591740694597 1104 40-41 0.5877682711231387 1104 42-43 0.6136964151537114 1104 44-45 0.4098911732723707 1104 46-47 0.4862939115559186 1104 48-49 0.43619376296473433 1104 50-51 0.641290897489597 1104 52-53 0.6510530551246845 1104 54-55 0.7063325632103883 1104 56-57 0.59736990579826 1104 58-59 0.6009977444992458 1104 60-61 0.5985591070045686 1104 62-63 0.5815271488185303 1104 64-65 0.6504749011843387 1104 66-67 0.6976874537354867 1104 68-69 0.5601334017652562 1104 70-71 0.5397121291604883 1104 72-73 0.3014737679810544 1104 74-75 0.3406422316625388 1104 76-77 0.22367584730194423 1104 78-79 0.26236938671988597 1104 80-81 0.31839948366645743 1104 82-83 0.29007722478526077 1104 84-85 0.33920123483276043 1104 86-87 0.4192792769874707 1104 88-89 0.3908393673015951 1104 90-91 0.28077161469405 1104 92-93 0.4442674266507609 1104 94-95 0.4517797777607022 1104 96-97 0.45918428528960575 1104 98-99 0.24699740996160813 1104 100-101 -4.725591776661986E-4 1105 1 0.25628751047172926 1105 2 0.25213077397945227 1105 3 0.19499773250704777 1105 4 0.20800884871454173 1105 5 0.12073747774130794 1105 6 0.24088055052962432 1105 7 0.22928139384667645 1105 8 0.285869945305123 1105 9 0.28385408328043127 1105 10-11 0.287917329839118 1105 12-13 0.3081476765035873 1105 14-15 0.5078335973029553 1105 16-17 0.40031461108178945 1105 18-19 0.3850095943462364 1105 20-21 0.3015557990564446 1105 22-23 0.3804747216506925 1105 24-25 0.4352461825997409 1105 26-27 0.3471708947057621 1105 28-29 0.4211553865851343 1105 30-31 0.43578790221844343 1105 32-33 0.4473354985479716 1105 34-35 0.49329024443780867 1105 36-37 0.42345527179973885 1105 38-39 0.43270176994727905 1105 40-41 0.4865544100989325 1105 42-43 0.4963867999551894 1105 44-45 0.5103266150038124 1105 46-47 0.23813674396998863 1105 48-49 0.2536485254070726 1105 50-51 0.19252894972110113 1105 52-53 0.4318215829015557 1105 54-55 0.3389079053815749 1105 56-57 0.45745011309003303 1105 58-59 0.3045904075748638 1105 60-61 0.3493138726724041 1105 62-63 0.34529302093843484 1105 64-65 0.3376385090072418 1105 66-67 0.45334765866189386 1105 68-69 0.3129159281874294 1105 70-71 0.42844278963420734 1105 72-73 0.44307081772892687 1105 74-75 0.435223938809159 1105 76-77 0.3138440030718108 1105 78-79 0.485410167719575 1105 80-81 0.4787543955567024 1105 82-83 0.5441988689272286 1105 84-85 0.4161518525149219 1105 86-87 0.4987979257203854 1105 88-89 0.41158037086038846 1105 90-91 0.40994836103042687 1105 92-93 0.6127747754019559 1105 94-95 0.6889729526147477 1105 96-97 0.6360972722048501 1105 98-99 0.48739457582727397 1105 100-101 -0.04236205374270696 1106 1 0.08872975912010261 1106 2 0.15401635002484682 1106 3 0.19822951027205704 1106 4 0.10571602909535471 1106 5 -0.0045709869248824475 1106 6 0.035378932406644026 1106 7 0.05217065295699541 1106 8 -0.011776595358590214 1106 9 0.002273876084096571 1106 10-11 0.0645123139770476 1106 12-13 0.08602949926965664 1106 14-15 0.15543896679032798 1106 16-17 0.042846032029942194 1106 18-19 0.25143242551887823 1106 20-21 0.2391485937814295 1106 22-23 0.16707781236132035 1106 24-25 0.1385494513996548 1106 26-27 0.03962890968058019 1106 28-29 0.1362971239449351 1106 30-31 0.1645170852418758 1106 32-33 0.11227233749526278 1106 34-35 -0.06947441601169402 1106 36-37 -0.3879009514310141 1106 38-39 -0.15519294673757855 1106 40-41 0.01949071286345827 1106 42-43 0.14696574967528875 1106 44-45 0.033572371819396096 1106 46-47 -0.237579283426399 1106 48-49 -0.06894296077663853 1106 50-51 0.07933502390581992 1106 52-53 -0.12970634647550838 1106 54-55 -0.3654744312263034 1106 56-57 0.04660039107525904 1106 58-59 -0.03225651862979362 1106 60-61 -0.33943139583387705 1106 62-63 -0.20847462043190035 1106 64-65 -0.5552995172018882 1106 66-67 -0.2829844682602243 1106 68-69 -0.9174342502209996 1106 70-71 -0.7423486544208693 1106 72-73 -0.4424729928708935 1106 74-75 -0.21826539817532264 1106 76-77 -0.2513906088020832 1106 78-79 -0.3172563840507827 1106 80-81 -0.8646713016404668 1106 82-83 -0.48533554570800774 1106 84-85 -0.3605979224798119 1106 86-87 -0.22924634765622542 1106 88-89 -0.01897789752461776 1106 90-91 -0.18071344169261394 1106 92-93 -0.3631043409177863 1106 94-95 -0.18527782772266121 1106 96-97 -0.17889446164423717 1106 98-99 -0.3185414141639811 1106 100-101 -0.724198862689299 1107 1 0.11613552980727704 1107 2 0.14824420644842462 1107 3 0.1748357153836153 1107 4 0.07262008954859311 1107 5 0.02903906309217774 1107 6 0.06649707586656461 1107 7 0.08790455553607757 1107 8 0.12379751893995916 1107 9 0.20969591688004385 1107 10-11 0.13666379659590433 1107 12-13 0.09944412072955089 1107 14-15 0.29643402334040303 1107 16-17 0.17635205321852965 1107 18-19 0.2401778586533112 1107 20-21 0.16420737006951214 1107 22-23 0.14864941748454186 1107 24-25 0.15200911734227418 1107 26-27 0.17854609441081237 1107 28-29 0.3468062202256945 1107 30-31 0.22831432473287805 1107 32-33 0.141035060220986 1107 34-35 0.14009342742907904 1107 36-37 0.1253816574455584 1107 38-39 0.09757057909069289 1107 40-41 0.1481613700759894 1107 42-43 0.135223287604191 1107 44-45 0.13984302154185002 1107 46-47 0.17646065532142785 1107 48-49 0.15144408888255612 1107 50-51 0.266246740880824 1107 52-53 0.16836402278521945 1107 54-55 0.07689692672441595 1107 56-57 -0.020323226797728466 1107 58-59 -0.0899033657240551 1107 60-61 -0.2275095880625102 1107 62-63 0.01869282020924601 1107 64-65 0.10751735460963374 1107 66-67 0.11211983104319856 1107 68-69 0.21064645950527705 1107 70-71 0.08141173805909929 1107 72-73 0.09803323221420257 1107 74-75 0.16862332842859473 1107 76-77 0.015835420875259132 1107 78-79 0.19013600852904844 1107 80-81 0.2863959301107961 1107 82-83 0.2539434512876362 1107 84-85 0.3088871972365652 1107 86-87 0.30690543872681886 1107 88-89 0.3079416424182959 1107 90-91 0.4137361001644244 1107 92-93 0.2459017468492526 1107 94-95 0.13124590333368857 1107 96-97 0.3294737213466341 1107 98-99 0.2687303465863593 1107 100-101 0.018412801855177463 1108 1 0.22344618431375807 1108 2 0.12473972606528605 1108 3 0.16532939423836268 1108 4 0.05060933583220617 1108 5 0.07160429659404599 1108 6 0.0684289796209967 1108 7 0.08030742744373498 1108 8 0.03200982814018971 1108 9 0.19502111818849954 1108 10-11 0.08964348047887682 1108 12-13 0.11078816594377372 1108 14-15 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-0.7764537912015896 2316 72-73 -0.748140507568511 2316 74-75 -0.46768321063661134 2316 76-77 -0.4386777673266984 2316 78-79 -0.48725371608825085 2316 80-81 -0.6079504181637283 2316 82-83 -0.5434275713585635 2316 84-85 -0.5669024132324658 2316 86-87 -0.37395663551032143 2316 88-89 -0.183462075553944 2316 90-91 -0.31242551136455177 2316 92-93 -0.28197922300337197 2316 94-95 -0.3688198338399218 2316 96-97 -0.09130763375640782 2316 98-99 -0.14823774285696345 2316 100-101 -0.2289011466557973 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 20.0 4 39.0 5 93.0 6 211.0 7 524.0 8 1213.0 9 2108.0 10 3627.0 11 5411.0 12 7894.0 13 10772.0 14 14200.0 15 18191.0 16 22502.0 17 27032.0 18 26322.0 19 23344.0 20 23913.0 21 25505.0 22 28051.0 23 32012.0 24 37222.0 25 43904.0 26 52793.0 27 63621.0 28 76329.0 29 91661.0 30 109154.0 31 130501.0 32 156787.0 33 192274.0 34 243861.0 35 318806.0 36 422787.0 37 491523.0 38 258001.0 39 12170.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.09014095114115 19.37113278670667 15.058513349408479 23.4802129127437 2 15.979108881517206 20.4381322449375 39.4018980550553 24.18086081848999 3 19.504427873074363 23.875515240355842 29.543159154635983 27.07689773193381 4 13.42056938214392 16.845112699041348 36.34714707122353 33.38717084759119 5 13.855511895415775 37.34069255518154 34.315546266418686 14.488249282983997 6 33.0054108719055 36.17887682214322 16.80554145365323 14.010170852298057 7 29.33416084416864 32.07432670047089 20.249719101312476 18.341793354048 8 26.361375236939978 35.61338119985933 19.338739946651103 18.686503616549587 9 26.2058807510266 16.79834668176448 19.77234262445106 37.22342994275786 10-11 25.070367558706312 26.56837622067374 27.24154126806846 21.119714952551487 12-13 26.090545868015607 23.567483934477817 27.547974671713316 22.793995525793257 14-15 22.644317374163776 25.253800621440014 25.677586133859066 26.42429587053714 16-17 22.50810420450366 28.480723054402564 26.44994825815917 22.561224482934605 18-19 22.528629476644223 27.10497508389978 28.46429947464717 21.902095964808822 20-21 22.88828402000819 26.269759566861282 28.76434499670856 22.077611416421966 22-23 22.76857848117693 26.137362315936553 28.609455678524142 22.48460352436237 24-25 22.750204202529236 26.37516682073262 28.52951821315096 22.34511076358718 26-27 22.243272336568857 26.775823903511608 28.49339106918871 22.48751269073082 28-29 22.343044706429772 27.107109981165834 28.365825236973595 22.184020075430798 30-31 22.458715793734633 26.59039760192201 28.290061599350725 22.66082500499263 32-33 22.220817635394514 26.519660218492447 28.310334718270884 22.94918742784215 34-35 22.907574898127923 26.627221322408694 28.206890012450934 22.258313767012446 36-37 22.952225945879068 26.25004370664637 28.1501901575322 22.64754018994236 38-39 22.424286336531733 26.63866183340094 28.124785134799595 22.81226669526773 40-41 22.877741816829587 26.397713816154457 28.17445214096901 22.55009222604695 42-43 22.55525242895852 26.534799032333385 27.970732640492287 22.93921589821581 44-45 22.55853864072245 26.6901034719319 28.178816335429737 22.57254155191592 46-47 22.956903316386395 26.913943804124692 27.501528048515624 22.627624830973293 48-49 22.64075360864879 26.892830174469857 27.5323747924779 22.934041424403457 50-51 22.865758332319118 26.999743139919484 27.492164758931438 22.64233376882996 52-53 23.06933003225544 26.844685719588135 27.410450305307123 22.675533942849306 54-55 22.648956993419137 26.984580326266094 27.551874641522172 22.814588038792596 56-57 22.72110643488294 27.004584481751166 27.46531884606617 22.808990237299714 58-59 22.78046330296512 26.694150247009297 27.37837641567223 23.147010034353354 60-61 22.485830670034986 27.035733809577923 27.549773364685503 22.928662155701588 62-63 22.44696545366399 27.029732562932402 27.700897194778612 22.822404788624997 64-65 22.225020189067845 27.009610735944474 27.526810611414405 23.238558463573273 66-67 22.28780826387487 26.915529776882874 27.43975676271303 23.356905196529226 68-69 22.666416976971675 26.672658995208547 27.574752673559694 23.086171354260088 70-71 22.547186304372676 26.77221807569981 27.25931118417956 23.421284435747953 72-73 22.552449550864683 26.842517201892697 27.212762180513465 23.392271066729155 74-75 22.344675297255026 27.174687044233053 27.13074514302467 23.34989251548725 76-77 22.475643679930823 26.778285371549455 27.32162513086752 23.424445817652202 78-79 22.459220100175433 26.937024228898643 27.41393001974864 23.189825651177287 80-81 22.539641874514704 27.050058189213537 27.139607211461602 23.270692724810154 82-83 22.542417442910523 27.0129331763475 27.180178901991216 23.264470478750766 84-85 22.358278954068915 27.030903090006404 27.19553285627181 23.41528509965287 86-87 22.513586280445573 27.14406702161567 26.905222833827114 23.437123864111644 88-89 22.380554058511038 27.234760600448027 26.768427964291035 23.616257376749903 90-91 22.372033810404933 27.051753333524974 26.92336981765237 23.65284303841772 92-93 22.37229760919755 26.8594859325898 27.058352443906365 23.709864014306287 94-95 22.20597980284288 26.744809588825095 27.12398117388537 23.925229434446653 96-97 22.220414190241872 26.554423742478267 27.165744010016198 24.05941805726366 98-99 22.068410548011595 26.778752752043662 26.787746221440063 24.365090478504683 100-101 22.153193865682663 26.63120046974472 27.024525939258297 24.19107972531432 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 548.0 1 367.0 2 279.5 3 390.0 4 353.5 5 357.5 6 610.0 7 1337.5 8 2039.5 9 2154.0 10 1793.0 11 1487.5 12 1523.0 13 1621.5 14 1767.5 15 1905.5 16 2094.5 17 2359.5 18 2620.0 19 2890.0 20 3321.5 21 3951.5 22 4698.5 23 5648.5 24 7008.0 25 8969.5 26 11693.5 27 15195.5 28 18937.0 29 23427.0 30 28877.0 31 35620.5 32 45645.5 33 51777.5 34 54402.0 35 59845.0 36 67206.0 37 74327.5 38 78631.5 39 82639.0 40 87949.5 41 94963.5 42 100040.5 43 104278.5 44 108941.5 45 113264.5 46 115280.5 47 118058.5 48 120664.0 49 117445.5 50 114407.0 51 110112.5 52 105212.5 53 103482.0 54 103234.5 55 97695.5 56 88777.0 57 81060.0 58 73910.5 59 65263.5 60 53266.5 61 43981.0 62 36801.0 63 27656.0 64 19727.0 65 14796.5 66 11061.0 67 8389.5 68 6415.5 69 4931.0 70 3744.5 71 2604.0 72 1888.0 73 1539.5 74 1150.0 75 829.0 76 605.5 77 371.5 78 224.5 79 133.5 80 81.0 81 49.5 82 25.5 83 9.5 84 5.5 85 2.5 86 2.0 87 2.0 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.051677289601672E-4 2 0.048547900034360075 3 0.0014120580342403901 4 0.0 5 0.090472575479545 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.185327910133937E-4 26-27 0.008825362714002439 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01038871268048287 36-37 2.0172257632005574E-4 38-39 0.00448832732312124 40-41 0.005648232136961561 42-43 3.5301450856009754E-4 44-45 1.6810214693337977E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 8.40510734666899E-5 68-69 7.900800905868849E-4 70-71 6.724085877335191E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0011262843844536447 82-83 5.379268701868153E-4 84-85 0.004454706893734564 86-87 0.0019499849044272053 88-89 1.0086128816002787E-4 90-91 0.003042648859494174 92-93 8.405107346668989E-4 94-95 1.0086128816002787E-4 96-97 0.0 98-99 5.0430644080013936E-5 100-101 1.6810214693337977E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2974382.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.54863269465332 #Duplication Level Percentage of deduplicated Percentage of total 1 70.92162571264916 34.43147956831085 2 15.106718568385466 14.6682106199609 3 5.645425748120126 8.222331031512674 4 2.753894634202865 5.347912763027663 5 1.5466009642686391 3.7542681069737407 6 0.9033765544258184 2.6314617915468337 7 0.61535475162738 2.0912242269567107 8 0.4253355571279188 1.6519567787983254 9 0.31565171871260134 1.3792013416092703 >10 1.6040908597261625 13.888636327737267 >50 0.10508643310115647 3.50133077567957 >100 0.05030678503283001 4.522984014747939 >500 0.003960719354804041 1.366050946644976 >1k 0.0024320206562568484 2.1017362096277923 >5k 1.3897260892896276E-4 0.4412154968656207 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7222 0.24280674103057376 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5857 0.19691485491776106 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4309 0.14487043022718668 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2991 0.10055870429554778 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2981 0.10022250000168101 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.6810214693337977E-5 0.0 14-15 0.0 0.0 0.0 3.3620429386675955E-5 0.0 16-17 0.0 0.0 0.0 3.3620429386675955E-5 0.0 18-19 0.0 0.0 0.0 3.3620429386675955E-5 0.0 20-21 0.0 0.0 0.0 6.724085877335191E-5 0.0 22-23 0.0 0.0 0.0 3.193940791734216E-4 0.0 24-25 0.0 0.0 0.0 8.06890305280223E-4 0.0 26-27 0.0 0.0 0.0 0.0015297295370937558 0.0 28-29 0.0 0.0 0.0 0.0042866047468011845 0.0 30-31 0.0 0.0 0.0 0.01455764592443069 0.0 32-33 0.0 0.0 0.0 0.035973859443743275 0.0 34-35 0.0 0.0 0.0 0.06561026794809813 0.0 36-37 0.0 0.0 0.0 0.11686461254808561 0.0 38-39 0.0 0.0 0.0 0.1951665925896539 0.0 40-41 0.0 0.0 0.0 0.2793521477738905 0.0 42-43 0.0 0.0 0.0 0.3689337818746886 0.0 44-45 0.0 0.0 0.0 0.4786204327487189 0.0 46-47 0.0 0.0 0.0 0.6079582245992613 0.0 48-49 0.0 0.0 0.0 0.7457347442258593 0.0 50-51 0.0 0.0 0.0 0.8909413787469128 0.0 52-53 0.0 0.0 0.0 1.0379467062401535 0.0 54-55 1.6810214693337977E-5 0.0 0.0 1.1981984829117445 0.0 56-57 3.3620429386675955E-5 0.0 0.0 1.390070273421504 0.0 58-59 3.3620429386675955E-5 0.0 0.0 1.5954574765447074 0.0 60-61 3.3620429386675955E-5 0.0 0.0 1.8199578937742362 0.0 62-63 3.3620429386675955E-5 0.0 0.0 2.0441557271392847 0.0 64-65 3.3620429386675955E-5 0.0 0.0 2.269967341114894 0.0 66-67 3.3620429386675955E-5 0.0 0.0 2.533770040297447 0.0 68-69 3.3620429386675955E-5 0.0 0.0 2.8280160382896344 0.0 70-71 3.3620429386675955E-5 0.0 0.0 3.1407364622298006 0.0 72-73 3.3620429386675955E-5 0.0 0.0 3.4627697451100765 3.3620429386675955E-5 74-75 3.3620429386675955E-5 0.0 0.0 3.794233558433315 3.3620429386675955E-5 76-77 3.3620429386675955E-5 0.0 0.0 4.156325582927815 3.3620429386675955E-5 78-79 3.3620429386675955E-5 0.0 0.0 4.554794912018698 3.3620429386675955E-5 80-81 3.3620429386675955E-5 0.0 0.0 4.981589452867857 3.3620429386675955E-5 82-83 3.3620429386675955E-5 0.0 0.0 5.41984856013787 6.724085877335191E-5 84-85 3.3620429386675955E-5 0.0 0.0 5.870126970913622 6.724085877335191E-5 86-87 3.3620429386675955E-5 0.0 0.0 6.332458305624496 6.724085877335191E-5 88-89 3.3620429386675955E-5 0.0 0.0 6.82893118637754 6.724085877335191E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6770 0.0 35.47624 1 GTATCAA 10040 0.0 33.679897 1 ATCAACG 12570 0.0 26.51198 3 TATCAAC 12785 0.0 26.07667 2 TCAACGC 12825 0.0 25.984844 4 CAACGCA 13085 0.0 25.468523 5 AACGCAG 13650 0.0 24.565323 6 TAAGGTG 1735 0.0 23.56451 5 GTACATG 10125 0.0 23.016321 1 TACATGG 10230 0.0 22.082735 2 ACGCAGA 15405 0.0 21.73591 7 CGCAGAG 15485 0.0 21.562273 8 GTAAGGT 1780 0.0 21.099226 4 ACATGGG 10145 0.0 21.087248 3 GAGTACT 8760 0.0 20.467428 12-13 AGTACTT 9075 0.0 20.254183 12-13 AGAGTAC 13985 0.0 19.884508 10-11 GTACTTT 8970 0.0 19.644089 14-15 GCAGAGT 16810 0.0 19.523638 9 AGGTAAG 1760 0.0 19.45605 2 >>END_MODULE