FastQCFastQC Report
Thu 26 May 2016
SRR938477_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938477_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2993544
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74550.24903592531126983No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55460.1852653577164725No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39720.1326855392805317No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA100300.035.208831
GGTATCA67100.034.5181961
ATCAACG124550.027.857543
TATCAAC126600.027.5566252
TCAACGC126500.027.3905454
CAACGCA128850.026.8172135
AACGCAG131400.026.183026
GTACATG105000.024.1466391
TACATGG105800.023.3604562
ACGCAGA150900.022.6989847
CGCAGAG151400.022.5298828
TAAGGTG17250.022.318225
ACATGGG106550.022.2592623
GTATAGG7600.021.947691
GTAAGGT16700.021.3456044
GGTAAGG17500.020.3698063
GCAGAGT161950.020.1814969
GAGTACT83250.019.91331112-13
AGAGTAC133200.019.89954610-11
GGACCGA10100.019.7607986
Produced by FastQC (version 0.11.5)