FastQCFastQC Report
Thu 26 May 2016
SRR938477_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938477_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2993544
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72400.24185380271677984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58460.19528692412738882No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44310.14801853588923364No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA30030.1003158797732721No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA107900.032.4043581
GGTATCA75050.030.234671
ATCAACG131200.026.1655733
TATCAAC132650.025.9996972
TCAACGC132600.025.81764
CAACGCA135000.025.393845
TAAGGTG17400.024.8667495
AACGCAG139750.024.5716536
GTAAGGT16350.023.264794
GTACATG98800.022.7259791
TACATGG97200.022.7085442
ACGCAGA157600.021.728367
CGCAGAG158150.021.5326678
GGTAAGG15900.021.5309053
GACCGTA1805.178046E-621.1088897
ACATGGG100800.020.9906753
GAGTACT83200.020.3223812-13
AGTACTT88300.020.27815812-13
AGAGTAC135050.019.97568110-11
GGACCGA12650.019.899096
Produced by FastQC (version 0.11.5)