FastQCFastQC Report
Thu 26 May 2016
SRR938476_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938476_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3000409
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67940.22643579591982294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52660.1755094055510432No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35710.11901710733436675No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63950.037.1551251
GTATCAA96450.034.57841
ATCAACG120950.027.4687563
TATCAAC122200.027.382252
TCAACGC122600.027.1378384
CAACGCA124550.026.6747955
AACGCAG129900.025.6429446
GTAAGGT13650.024.7225574
GTACATG103800.024.361751
TACATGG104000.023.8563962
ACGCAGA144450.022.9559637
TAAGGTG16150.022.9556585
CGCAGAG144950.022.7456768
ACATGGG107250.022.3800873
TCGCGTA1302.9420952E-421.9241169
GAGTACT79950.020.79419112-13
AGAGTAC127950.020.49198310-11
GCAGAGT158200.020.2680289
GGTAAGG15350.019.5074213
AGTACTT85550.019.21093612-13
Produced by FastQC (version 0.11.5)