##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938476_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3000409 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.788605153497407 31.0 31.0 34.0 27.0 34.0 2 30.96726612938436 31.0 31.0 34.0 27.0 34.0 3 31.016632732404148 31.0 31.0 34.0 27.0 34.0 4 34.68167173208719 37.0 35.0 37.0 32.0 37.0 5 34.659353774768704 37.0 35.0 37.0 32.0 37.0 6 34.624083249983585 37.0 35.0 37.0 30.0 37.0 7 34.52487977472405 37.0 35.0 37.0 30.0 37.0 8 34.46666471137768 37.0 35.0 37.0 30.0 37.0 9 35.90777124052087 38.0 35.0 39.0 30.0 39.0 10-11 35.71747701729998 38.0 35.0 39.0 30.0 39.0 12-13 35.52064718510043 38.0 35.0 39.0 30.0 39.0 14-15 36.620253272137234 39.0 36.0 41.0 30.0 41.0 16-17 36.535075384722546 39.0 36.0 41.0 30.0 41.0 18-19 36.512644276163684 39.0 36.0 41.0 29.5 41.0 20-21 36.42776751436221 39.0 36.0 41.0 29.0 41.0 22-23 36.253645253030506 39.0 35.5 40.5 28.5 41.0 24-25 36.1005174627859 39.0 35.0 40.5 27.0 41.0 26-27 35.865912447269686 39.0 35.0 40.0 27.0 41.0 28-29 35.6094462454952 38.0 34.0 40.0 27.0 41.0 30-31 35.32283498683013 38.0 34.0 40.0 26.5 41.0 32-33 35.01038341772738 38.0 34.0 40.0 25.0 41.0 34-35 34.68640392026553 38.0 33.5 40.0 24.5 41.0 36-37 34.43609254604955 38.0 33.0 40.0 24.0 41.0 38-39 34.16137333276897 38.0 33.0 40.0 23.5 41.0 40-41 33.86656369181668 38.0 33.0 40.0 22.5 41.0 42-43 33.425720293466654 37.5 32.0 40.0 19.5 41.0 44-45 32.93235172271514 37.0 31.0 40.0 17.0 41.0 46-47 32.42853457645275 37.0 30.5 40.0 14.0 41.0 48-49 31.927869167170208 36.5 30.0 39.0 9.0 40.5 50-51 31.214060483087472 35.5 29.5 38.5 8.5 40.0 52-53 31.221721105355968 36.0 30.0 39.0 7.5 40.0 54-55 31.15732138518449 36.0 29.5 39.0 4.0 40.0 56-57 30.686875022705237 35.5 28.5 39.0 2.0 40.0 58-59 30.188553793832774 35.0 28.0 39.0 2.0 40.0 60-61 29.598805362868863 34.5 27.0 38.0 2.0 40.0 62-63 28.833748998886485 34.0 26.0 38.0 2.0 40.0 64-65 28.125456062823435 34.0 24.5 38.0 2.0 40.0 66-67 27.38720587759869 33.0 22.5 37.0 2.0 39.0 68-69 26.48626387269202 32.5 20.5 36.0 2.0 39.0 70-71 25.602613843645983 31.5 17.0 36.0 2.0 39.0 72-73 24.655747933031797 31.0 9.5 35.0 2.0 38.0 74-75 23.68232014368708 30.0 4.5 35.0 2.0 37.0 76-77 22.692734390544757 29.5 2.0 34.0 2.0 36.5 78-79 21.67249431660817 28.5 2.0 34.0 2.0 36.0 80-81 20.680877840321102 27.0 2.0 34.0 2.0 35.5 82-83 19.65560328608533 26.0 2.0 33.0 2.0 35.0 84-85 18.660158498391386 24.5 2.0 32.5 2.0 35.0 86-87 17.655980234694667 21.5 2.0 32.0 2.0 34.0 88-89 16.557662472016315 17.5 2.0 31.0 2.0 34.0 90-91 15.402617776443144 4.5 2.0 31.0 2.0 34.0 92-93 14.258369608943314 2.0 2.0 30.0 2.0 34.0 94-95 13.04111356151778 2.0 2.0 29.0 2.0 34.0 96-97 11.698066496934251 2.0 2.0 28.0 2.0 34.0 98-99 10.110738236020488 2.0 2.0 24.5 2.0 33.0 100-101 7.186534402476463 2.0 2.0 2.0 2.0 28.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7786151709219382 1101 2 0.6783795778724517 1101 3 0.6279173590499667 1101 4 0.5250135775361002 1101 5 0.4811557419043382 1101 6 0.565681636639674 1101 7 0.5037952153168561 1101 8 0.55646165131256 1101 9 0.7076451879780166 1101 10-11 0.8445488653028974 1101 12-13 0.7128384032270603 1101 14-15 0.8663646812014392 1101 16-17 0.9594668361862446 1101 18-19 0.9063066016791623 1101 20-21 0.9656809407243756 1101 22-23 1.0689342418158319 1101 24-25 1.1127125208276212 1101 26-27 1.1828752524132753 1101 28-29 1.1741719126419383 1101 30-31 1.2742618698738681 1101 32-33 1.2154008593549008 1101 34-35 1.4282780398339199 1101 36-37 1.3828270663441984 1101 38-39 1.4263519354265384 1101 40-41 1.507092224543463 1101 42-43 1.5437068932830442 1101 44-45 1.6497104109583418 1101 46-47 1.7303476681689318 1101 48-49 1.4365415171266456 1101 50-51 1.740557641433984 1101 52-53 1.7969465572009256 1101 54-55 2.0461832478263133 1101 56-57 2.2698502437097865 1101 58-59 2.108933358837181 1101 60-61 2.206961228574041 1101 62-63 2.24571964910459 1101 64-65 2.23379447134624 1101 66-67 2.63101763213594 1101 68-69 2.6571822610008304 1101 70-71 2.8241108037919 1101 72-73 2.955663691059865 1101 74-75 2.9400498175593412 1101 76-77 3.129419446070692 1101 78-79 3.2275603099140007 1101 80-81 3.579595585551626 1101 82-83 3.6377582572788256 1101 84-85 3.7510596839974646 1101 86-87 4.026437263047228 1101 88-89 4.380252281314657 1101 90-91 4.274037248949066 1101 92-93 4.155536395260972 1101 94-95 4.058350745966402 1101 96-97 3.9809017235917583 1101 98-99 3.6157128432608676 1101 100-101 2.774250556920175 1102 1 0.7994237106307658 1102 2 0.8113204376851755 1102 3 0.712882947711968 1102 4 0.5369397434298833 1102 5 0.6984281575537068 1102 6 0.6885163535880139 1102 7 0.8762924573404547 1102 8 0.8560708273474944 1102 9 0.9899412727378092 1102 10-11 1.0225392479917446 1102 12-13 1.0759262987884668 1102 14-15 1.0948509564969626 1102 16-17 1.260280046953575 1102 18-19 1.2229945918687335 1102 20-21 1.2167583620103954 1102 22-23 1.1011373882022113 1102 24-25 1.280805811642658 1102 26-27 1.2225954837694815 1102 28-29 1.2712719939931887 1102 30-31 1.2658432826819848 1102 32-33 1.3000763475227615 1102 34-35 1.222854602948729 1102 36-37 1.3913830092100312 1102 38-39 1.5562852732659707 1102 40-41 1.7313877637429371 1102 42-43 1.9392266601887727 1102 44-45 1.7830253175492388 1102 46-47 1.8071713725235128 1102 48-49 1.8128515860941583 1102 50-51 1.9862978050538054 1102 52-53 1.9793888086752673 1102 54-55 2.0500289049488885 1102 56-57 2.4057963322112563 1102 58-59 2.5863341260012085 1102 60-61 2.5026825631208034 1102 62-63 2.562415967538012 1102 64-65 2.972682783561236 1102 66-67 3.0777694963348736 1102 68-69 3.126090995518144 1102 70-71 3.077313194601377 1102 72-73 3.386036011839682 1102 74-75 3.3024436927998337 1102 76-77 3.4038078632939843 1102 78-79 4.050602448413901 1102 80-81 4.288672707503668 1102 82-83 4.390977485624788 1102 84-85 4.333161744815371 1102 86-87 4.516512772458142 1102 88-89 4.729872380625945 1102 90-91 4.357702243529674 1102 92-93 4.456397402906054 1102 94-95 4.414019866045196 1102 96-97 4.28990630533287 1102 98-99 3.6047380283530703 1102 100-101 2.5496038434383186 1103 1 0.5693729933215295 1103 2 0.5515158367056827 1103 3 0.5436871926072726 1103 4 0.5034880445365388 1103 5 0.5083628483442553 1103 6 0.6398330808896802 1103 7 0.638128713216858 1103 8 0.5127812716511002 1103 9 0.6739069701545475 1103 10-11 0.656327397068452 1103 12-13 0.6943908033522135 1103 14-15 0.8344970680634347 1103 16-17 0.8957675250468569 1103 18-19 1.012745931250656 1103 20-21 0.9043469401308002 1103 22-23 1.0743186722313496 1103 24-25 1.0418449152631624 1103 26-27 1.0949511520430733 1103 28-29 1.1085952516349096 1103 30-31 1.091179224588565 1103 32-33 1.2817985983698321 1103 34-35 1.2314682388238936 1103 36-37 1.343017888731012 1103 38-39 1.4096405099399618 1103 40-41 1.5243982319210332 1103 42-43 1.6670827835879223 1103 44-45 1.719610615396526 1103 46-47 1.7661265994386 1103 48-49 1.7100790669640737 1103 50-51 1.7847291176916578 1103 52-53 2.022511010611602 1103 54-55 2.181089553695948 1103 56-57 2.309853019884759 1103 58-59 2.3899416712683355 1103 60-61 2.6430205700833262 1103 62-63 2.6716667791834148 1103 64-65 2.8330674590453917 1103 66-67 3.150967277576033 1103 68-69 3.227623145040976 1103 70-71 3.385546987978447 1103 72-73 3.6387437329415313 1103 74-75 3.5307929898966144 1103 76-77 3.678728325239156 1103 78-79 3.8743490233545224 1103 80-81 3.824432621950521 1103 82-83 3.929929668688075 1103 84-85 3.924511629189663 1103 86-87 4.073994229069022 1103 88-89 4.471037048941948 1103 90-91 4.306641861990984 1103 92-93 4.262450195146327 1103 94-95 4.01982392215198 1103 96-97 3.933936144374682 1103 98-99 3.4791710673893075 1103 100-101 2.274043882171914 1104 1 0.7151752798748845 1104 2 0.5116112639432444 1104 3 0.5267065682411847 1104 4 0.4628016454166257 1104 5 0.6069852458703053 1104 6 0.6670749540878802 1104 7 0.633216755860154 1104 8 0.6371559632655845 1104 9 0.7631942054954948 1104 10-11 0.7467765440947858 1104 12-13 0.8155320210711992 1104 14-15 0.9346948901217544 1104 16-17 0.9246786086237577 1104 18-19 0.8172318709809474 1104 20-21 0.8161192238755746 1104 22-23 0.9555520854908792 1104 24-25 1.0183583628430952 1104 26-27 1.0599786930981168 1104 28-29 1.0613316827552808 1104 30-31 1.03124951344342 1104 32-33 1.1377666376637166 1104 34-35 1.0231006867055612 1104 36-37 1.2953782880266118 1104 38-39 1.3899809642232839 1104 40-41 1.2985404245908043 1104 42-43 1.3778546738652508 1104 44-45 1.3671654034888192 1104 46-47 1.556558655118856 1104 48-49 1.6608165102386288 1104 50-51 1.5921048719902835 1104 52-53 1.9340604072115681 1104 54-55 2.114385272741316 1104 56-57 2.2426328334835866 1104 58-59 2.4250738826586407 1104 60-61 2.5871084913436384 1104 62-63 2.532699753931645 1104 64-65 2.472806759934933 1104 66-67 2.7160272799725966 1104 68-69 3.0869080049180333 1104 70-71 3.2547011840149658 1104 72-73 3.401998493836487 1104 74-75 3.3471570264616552 1104 76-77 3.4131857782820987 1104 78-79 3.6859179878244 1104 80-81 3.898254965459479 1104 82-83 3.810152649118983 1104 84-85 3.9955151777262543 1104 86-87 3.9941287787198263 1104 88-89 4.181033165352783 1104 90-91 4.060354454403916 1104 92-93 4.049116268779931 1104 94-95 4.175112183417914 1104 96-97 3.852902840904008 1104 98-99 3.5104218551848643 1104 100-101 2.1055042749485926 1105 1 0.5836638100418625 1105 2 0.4998665503871038 1105 3 0.4939971547000752 1105 4 0.38605021478359447 1105 5 0.48952978378959244 1105 6 0.520907545517808 1105 7 0.599036604975197 1105 8 0.5393793336340096 1105 9 0.5725535926130831 1105 10-11 0.6610081781647068 1105 12-13 0.6294465939344605 1105 14-15 0.7212122633515534 1105 16-17 0.7505770453250236 1105 18-19 0.8546780200079382 1105 20-21 0.8091809672294801 1105 22-23 0.7629138984713535 1105 24-25 0.86702139788256 1105 26-27 0.754349533922678 1105 28-29 0.8325394854838066 1105 30-31 0.8998730597016547 1105 32-33 0.942717765211448 1105 34-35 0.994449502727214 1105 36-37 1.1107612267567148 1105 38-39 1.1543638142074428 1105 40-41 1.4150080874464592 1105 42-43 1.417030894422318 1105 44-45 1.342200390679686 1105 46-47 1.5645986472855995 1105 48-49 1.4575325521106883 1105 50-51 1.434665780478884 1105 52-53 1.6330553647136483 1105 54-55 1.8113153061591163 1105 56-57 1.9260957232410938 1105 58-59 1.9329805451177755 1105 60-61 2.3162678459561796 1105 62-63 2.2479905528748745 1105 64-65 2.290507316799893 1105 66-67 2.588754113856851 1105 68-69 2.7607235680890163 1105 70-71 2.794224272609764 1105 72-73 2.8367210067842272 1105 74-75 2.793552265463326 1105 76-77 2.7996299730012275 1105 78-79 3.1416369258495926 1105 80-81 3.267635065584294 1105 82-83 3.5743404055942207 1105 84-85 3.5239480804204426 1105 86-87 3.4777246925279144 1105 88-89 3.546470869392028 1105 90-91 3.4794337563844255 1105 92-93 3.5787552519833365 1105 94-95 3.5895811759870764 1105 96-97 3.5951925367415267 1105 98-99 3.485277611413048 1105 100-101 2.4968935789337925 1106 1 0.33626482074396336 1106 2 0.26235828396992034 1106 3 0.12139426044501533 1106 4 0.31336276762915105 1106 5 0.22562404009147485 1106 6 -0.1947727224020781 1106 7 0.2890324549491652 1106 8 -0.050484357156967974 1106 9 0.27534135448645003 1106 10-11 0.31538485149970086 1106 12-13 0.4988273189781651 1106 14-15 0.6342001372031731 1106 16-17 0.11396165545543369 1106 18-19 0.47303810772768884 1106 20-21 0.6316474591327932 1106 22-23 0.559885466135924 1106 24-25 0.5826340338321359 1106 26-27 0.5863416292097696 1106 28-29 0.5661852264290417 1106 30-31 0.4836939607230235 1106 32-33 0.47818439475901187 1106 34-35 0.33668791913523677 1106 36-37 0.5873826088085181 1106 38-39 0.7044352236328706 1106 40-41 0.8801655659279035 1106 42-43 0.3658444630410784 1106 44-45 0.9469182768779021 1106 46-47 0.9419885085960757 1106 48-49 0.7886031035880912 1106 50-51 0.7201392404932285 1106 52-53 0.8323580361505947 1106 54-55 0.768240778522113 1106 56-57 1.3436203724293598 1106 58-59 1.3988066331542157 1106 60-61 1.6120919986936961 1106 62-63 1.6702368657164648 1106 64-65 1.7383992381170579 1106 66-67 1.7011785259451209 1106 68-69 1.8726040266410209 1106 70-71 1.8747973384233774 1106 72-73 2.0048865814386367 1106 74-75 2.419232750716244 1106 76-77 2.5604396523936046 1106 78-79 2.8838519013375254 1106 80-81 2.7359779218992166 1106 82-83 2.8238999439788586 1106 84-85 2.59004573125733 1106 86-87 2.4808564615463666 1106 88-89 2.64530591258438 1106 90-91 2.829957477250156 1106 92-93 2.7966544384333485 1106 94-95 2.668916581918614 1106 96-97 2.6100081296622655 1106 98-99 2.351045019530334 1106 100-101 1.5332593980361233 1107 1 0.2776375146642174 1107 2 0.19720093682995454 1107 3 0.20979831196196486 1107 4 0.23251166092748576 1107 5 0.21442559646074955 1107 6 0.31183464345200207 1107 7 0.28655282260211834 1107 8 0.21004639821307336 1107 9 0.37351109928440707 1107 10-11 0.3791520147074081 1107 12-13 0.35541254525771393 1107 14-15 0.3539048119025594 1107 16-17 0.35311424623903065 1107 18-19 0.39180652274069416 1107 20-21 0.5021746228039419 1107 22-23 0.5011509128339569 1107 24-25 0.48321636257282563 1107 26-27 0.5910918399009404 1107 28-29 0.6631605424406217 1107 30-31 0.5009865552242516 1107 32-33 0.7483930407288284 1107 34-35 0.687633724781513 1107 36-37 0.7243649167949329 1107 38-39 0.8393753510701742 1107 40-41 0.7977264044208354 1107 42-43 0.9565840759536641 1107 44-45 0.9055206979751276 1107 46-47 0.9470220756165233 1107 48-49 0.8862603890193448 1107 50-51 1.1266540180925126 1107 52-53 1.2654272561978246 1107 54-55 1.4143691739495239 1107 56-57 1.4732749598046162 1107 58-59 1.5699471562325193 1107 60-61 1.9347766752367797 1107 62-63 2.1161210457688817 1107 64-65 2.1142243339670586 1107 66-67 2.1786494561044627 1107 68-69 2.171157322566284 1107 70-71 2.29635780817528 1107 72-73 2.237924871568321 1107 74-75 2.444981504074331 1107 76-77 2.4170062401234986 1107 78-79 2.6443889535148877 1107 80-81 2.604135118419549 1107 82-83 2.6466489487054936 1107 84-85 2.636836097444384 1107 86-87 2.72823122182691 1107 88-89 2.791189840764506 1107 90-91 2.566829062176703 1107 92-93 2.638262481823892 1107 94-95 2.5318237019957053 1107 96-97 2.5004621193949426 1107 98-99 2.253697839265838 1107 100-101 1.595902253703728 1108 1 0.34494624229171933 1108 2 0.16100949164911782 1108 3 0.08096981537907055 1108 4 0.06766863164122583 1108 5 0.07061033403203965 1108 6 0.21253614198373327 1108 7 0.1112640385731396 1108 8 0.2016668112476978 1108 9 0.26749745597182795 1108 10-11 0.21108501625259635 1108 12-13 0.1730277071205606 1108 14-15 0.3014385776092112 1108 16-17 0.41768227898483445 1108 18-19 0.44481923504169885 1108 20-21 0.3137046038196445 1108 22-23 0.3185085762682007 1108 24-25 0.28005652417735405 1108 26-27 0.4037976805138044 1108 28-29 0.4074590019018274 1108 30-31 0.4991407072162062 1108 32-33 0.4784551638543135 1108 34-35 0.5332485212312434 1108 36-37 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66-67 -0.938459836021071 2313 68-69 -1.01737233309548 2313 70-71 -1.2935746261772003 2313 72-73 -1.3290731602187371 2313 74-75 -1.3713348386758284 2313 76-77 -1.3324682189863069 2313 78-79 -1.479989599345803 2313 80-81 -1.2323637550079312 2313 82-83 -1.6074352773369363 2313 84-85 -1.5957932105868302 2313 86-87 -1.5881659696879282 2313 88-89 -1.3910018936596416 2313 90-91 -1.4437512185895276 2313 92-93 -1.366388379244155 2313 94-95 -1.376824130640271 2313 96-97 -1.077065865998314 2313 98-99 -0.8468836690621107 2313 100-101 -0.32733849392058545 2314 1 -0.5121525379668057 2314 2 -0.42939388280563406 2314 3 -0.30287402752233206 2314 4 -0.3155820092854924 2314 5 -0.39104809195340096 2314 6 -0.24066131003954183 2314 7 -0.2773841434630455 2314 8 -0.43367271986859635 2314 9 -0.49837943315070987 2314 10-11 -0.42858646807025025 2314 12-13 -0.6268154823152514 2314 14-15 -0.6517630139280044 2314 16-17 -0.6361204758713228 2314 18-19 -0.4868101941299159 2314 20-21 -0.6827336332364524 2314 22-23 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-0.9521768534909221 2314 88-89 -0.9659455016763872 2314 90-91 -1.054060032716837 2314 92-93 -1.002633070423828 2314 94-95 -1.1745548689710716 2314 96-97 -1.2437551222059646 2314 98-99 -1.1048657493404122 2314 100-101 -0.6517031118128562 2315 1 -0.7078920810803666 2315 2 -0.5660662828148482 2315 3 -0.5086602412385339 2315 4 -0.6730725849027124 2315 5 -0.5011654761868982 2315 6 -0.4120540634279166 2315 7 -0.5107532010917026 2315 8 -0.4946598797930193 2315 9 -1.3693413294887051 2315 10-11 -0.9997669132314542 2315 12-13 -0.8413084594792011 2315 14-15 -0.7248812429059086 2315 16-17 -0.7213146027026767 2315 18-19 -0.6406035807911437 2315 20-21 -0.5373078621734066 2315 22-23 -0.5274078249493144 2315 24-25 -0.7080561230226223 2315 26-27 -0.788616704166202 2315 28-29 -0.9374662431315528 2315 30-31 -0.9869437816160982 2315 32-33 -1.0397159347884326 2315 34-35 -0.9069027872380957 2315 36-37 -0.9171649617382656 2315 38-39 -0.9418346287613915 2315 40-41 -0.9298989828090001 2315 42-43 -0.8743784342139875 2315 44-45 -1.001256167388071 2315 46-47 -1.1385453766772144 2315 48-49 -1.1160006363303268 2315 50-51 -1.0153195937007204 2315 52-53 -0.9903209359396676 2315 54-55 -0.9893747242244544 2315 56-57 -0.9870829929242682 2315 58-59 -0.8664100997936721 2315 60-61 -0.9519190878511274 2315 62-63 -0.9116473353432859 2315 64-65 -0.8329782210834153 2315 66-67 -1.123755303670361 2315 68-69 -1.0253309048763377 2315 70-71 -1.1482492923862893 2315 72-73 -1.0956085070664905 2315 74-75 -1.018451577415199 2315 76-77 -0.9600622194072201 2315 78-79 -0.9970184886047768 2315 80-81 -1.2274656902927106 2315 82-83 -1.380382740405544 2315 84-85 -1.6141972560119662 2315 86-87 -1.285075740282771 2315 88-89 -1.3753232866900191 2315 90-91 -1.1939337403770018 2315 92-93 -1.2587043140131904 2315 94-95 -1.1975913303916883 2315 96-97 -1.20377485193894 2315 98-99 -1.1976652334773874 2315 100-101 -0.7139686588207859 2316 1 -1.0885313840391788 2316 2 -0.9935678199655094 2316 3 -0.942111817456091 2316 4 -0.8928478154445614 2316 5 -0.7208464992290899 2316 6 -0.7760002895000895 2316 7 -1.0272750848070444 2316 8 -0.8120436134894362 2316 9 -0.8521646062654185 2316 10-11 -1.0087607566329666 2316 12-13 -1.0391600466859217 2316 14-15 -1.2390527571412875 2316 16-17 -1.421847362002417 2316 18-19 -1.194850083786207 2316 20-21 -1.2499042557175954 2316 22-23 -1.3404314120879732 2316 24-25 -1.2609584520903425 2316 26-27 -1.4537123744961207 2316 28-29 -1.4038380099127465 2316 30-31 -1.2950594040488497 2316 32-33 -1.6464350924934266 2316 34-35 -1.2506493467166493 2316 36-37 -1.546082953238475 2316 38-39 -1.576664525280762 2316 40-41 -1.4794997951872304 2316 42-43 -1.3558061278565319 2316 44-45 -1.448899913783528 2316 46-47 -0.9331266925109851 2316 48-49 -0.9416150711745139 2316 50-51 -1.0996335632070107 2316 52-53 -1.0425584819439884 2316 54-55 -1.1214738846398369 2316 56-57 -1.0510947849559322 2316 58-59 -0.9744131037725303 2316 60-61 -1.0401102238992657 2316 62-63 -0.83978054454278 2316 64-65 -0.7581427151686384 2316 66-67 -0.6408603205323722 2316 68-69 -0.6571581551798253 2316 70-71 -0.7042610327524699 2316 72-73 -0.8201727384368276 2316 74-75 -0.7330369895837734 2316 76-77 -0.8312730328290101 2316 78-79 -0.7952463214981016 2316 80-81 -0.8663265709308874 2316 82-83 -0.8616239667555163 2316 84-85 -0.6622936187003852 2316 86-87 -0.559499829027672 2316 88-89 -0.7597289419282944 2316 90-91 -0.7247466660149939 2316 92-93 -0.7625857860120355 2316 94-95 -0.7464688259934587 2316 96-97 -0.7156812857473547 2316 98-99 -0.7072316001493792 2316 100-101 -0.2742990288379117 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13451.0 3 5285.0 4 5876.0 5 6633.0 6 7701.0 7 9623.0 8 12322.0 9 15682.0 10 19643.0 11 24295.0 12 29106.0 13 33252.0 14 37853.0 15 43021.0 16 48050.0 17 54169.0 18 54926.0 19 56693.0 20 61708.0 21 67896.0 22 75652.0 23 82705.0 24 91648.0 25 99498.0 26 108877.0 27 118411.0 28 128409.0 29 140596.0 30 153077.0 31 168466.0 32 184166.0 33 203007.0 34 219866.0 35 230074.0 36 215097.0 37 141399.0 38 31873.0 39 403.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.18467054007438 18.534874889377015 14.42741208295762 23.853042487590987 2 15.462697056397653 19.683169518343256 40.272950156724974 24.581183268534122 3 19.477521000348407 23.596973349270094 29.01583840525713 27.90966724512437 4 12.902796644586664 16.128270737762804 36.67547599375011 34.29345662390042 5 13.988952000832402 37.239307505756116 34.016218494793485 14.755521998617999 6 33.08075612432761 35.54866098954289 17.16692471269523 14.203658173434274 7 29.446923714248086 31.864342358171783 20.303402610262676 18.385331317317455 8 26.435621545584365 34.97388172540986 19.569451071120994 19.021045657884777 9 26.52276093887685 16.68046400395283 19.81278741442789 36.98398764274243 10-11 25.09892196067527 26.306556722259007 27.27212107754588 21.32240023951984 12-13 26.174152525562665 23.46996538525778 27.426624114170306 22.929257975009243 14-15 22.80882303180079 25.054202581554737 25.62098981323175 26.51598457341272 16-17 22.649040841532585 28.24512920178747 26.388017061336473 22.71781289534347 18-19 22.630418711719077 27.005543206758865 28.32459471916003 22.039443362362036 20-21 23.018854965942303 26.174924247235822 28.580809033020156 22.225411753801723 22-23 22.78030187477756 26.086371626538106 28.3958495343687 22.737476964315633 24-25 22.687234070220633 26.246929543633573 28.433051130871544 22.63278525527425 26-27 22.23915565058428 26.606318684158147 28.38780166350651 22.766724001751058 28-29 22.35900361196663 27.018253867178043 28.23718425297235 22.385558267882967 30-31 22.458491776038045 26.542275216957663 28.127588559685172 22.871644447319117 32-33 22.2150840978109 26.443278192711357 28.224316170830022 23.11732153864772 34-35 22.95655287479082 26.592946516190302 28.035388606054383 22.415112002964495 36-37 22.935980772327515 26.146043894383826 27.991496375807777 22.926478957480878 38-39 22.385781810424138 26.643255243403246 27.997089961199485 22.973872984973134 40-41 22.903185215493497 26.392564080555793 28.00967989513447 22.694570808816238 42-43 22.56195975216099 26.443127560823086 27.835938656245375 23.158974030770544 44-45 22.582942413006858 26.63043650357816 27.97451800011233 22.812103083302652 46-47 22.93596468989905 26.86889869947262 27.365294901713316 22.829841708915012 48-49 22.723553678166837 26.790023639030686 27.413358694081474 23.073063988721003 50-51 22.91450042123561 27.006177288942627 27.288084323785604 22.79123796603616 52-53 23.01095712246292 26.77843667097867 27.34657512010237 22.864031086456038 54-55 22.613026132861183 26.903917745966975 27.499867507463748 22.983188613708094 56-57 22.68319384857116 26.943316305842863 27.38486111604142 22.988628729544555 58-59 22.741838019946787 26.58902510196538 27.379200562080307 23.289936316007523 60-61 22.491573457369817 26.930005324658335 27.50240192920954 23.076019288762303 62-63 22.49123102917368 26.892516067141166 27.678948503483426 22.93730440020173 64-65 22.305266201533723 26.907215893514387 27.493140080677485 23.294377824274406 66-67 22.35998594809996 26.715858315785123 27.441500966901376 23.48265476921354 68-69 22.71776361257456 26.48197314107261 27.563539680188008 23.23672356616481 70-71 22.56858391519231 26.546869328145796 27.29769990843367 23.586846848228223 72-73 22.632801808884192 26.507763205766246 27.2822588319684 23.577176153381167 74-75 22.460195716477102 26.834100319527582 27.231176505489568 23.474527458505747 76-77 22.68967950381278 26.43578168868908 27.279914580124213 23.594624227373924 78-79 22.678840461842036 26.47193816329796 27.29582755586369 23.55339381899632 80-81 22.72766817285475 26.5637312220923 27.066653155861804 23.641947449191154 82-83 22.659574060287156 26.494824793989252 27.107652918283527 23.737948227440068 84-85 22.65580132945224 26.38559047898078 27.176692221774474 23.781915969792507 86-87 22.731032156581207 26.485696844801804 26.878003102654052 23.905267895962933 88-89 22.72016195055348 26.40681709694366 26.788326253163408 24.08469469933945 90-91 22.676582528194647 26.285034013265264 26.869671128704343 24.168712329835746 92-93 22.76448752612214 25.931826367180044 27.04425984624792 24.259426260449896 94-95 22.6159525539014 25.79788271351628 27.062940886057422 24.5232238465249 96-97 22.703363820586013 25.617859394172942 26.998052891610968 24.68072389363008 98-99 22.60769886267521 25.667514370098125 26.616076676966994 25.108710090259667 100-101 23.50980554102543 24.631992918753372 26.85003825107891 25.00816328914228 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 554.0 1 396.0 2 303.0 3 389.0 4 356.5 5 351.0 6 549.0 7 1054.0 8 1681.5 9 1918.0 10 1699.5 11 1464.0 12 1420.0 13 1555.0 14 1747.0 15 1909.0 16 2075.0 17 2279.0 18 2502.0 19 2828.5 20 3347.5 21 3998.0 22 4801.0 23 5869.0 24 7312.5 25 9441.5 26 12250.5 27 15575.5 28 19221.5 29 23586.0 30 28941.0 31 35628.0 32 44006.5 33 49740.5 34 53677.0 35 59169.0 36 66219.5 37 72969.5 38 77321.0 39 81780.0 40 87358.5 41 93747.5 42 98396.0 43 102202.5 44 106710.0 45 110581.0 46 113081.5 47 115416.0 48 117220.0 49 116010.0 50 112798.5 51 109255.5 52 106289.5 53 103845.0 54 101949.5 55 97611.5 56 90843.5 57 83701.5 58 77388.5 59 69235.0 60 58042.5 61 48615.0 62 40715.5 63 32356.5 64 24667.5 65 19257.5 66 14995.5 67 11406.0 68 8732.0 69 6722.0 70 5150.0 71 3825.0 72 2891.0 73 2217.5 74 1704.5 75 1257.0 76 886.5 77 613.0 78 416.0 79 261.5 80 151.0 81 100.5 82 61.0 83 33.5 84 24.0 85 14.5 86 9.5 87 10.5 88 6.5 89 2.5 90 2.5 91 2.0 92 1.5 93 2.5 94 2.0 95 0.0 96 0.5 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38841371293047044 2 0.029562636293918598 3 0.035128544141815335 4 0.04382735820349826 5 0.06189156211703138 6 0.05299277531829827 7 0.03646169572214988 8 0.0389280261457688 9 0.03459528350968151 10-11 0.03582844872149097 12-13 0.027329607396858226 14-15 0.02607977779029459 16-17 0.02146374044338622 18-19 0.026379736895869862 20-21 0.033778728166726606 22-23 0.032878850850000786 24-25 0.04206093235955498 26-27 0.036061750248049515 28-29 0.031162418190320056 30-31 0.03444530395689388 32-33 0.02886273171424296 34-35 0.030412520426381866 36-37 0.03272887129721315 38-39 0.014964626489255298 40-41 0.012548289249898929 42-43 0.013231529434820387 44-45 0.0110151649325142 46-47 0.019580663836163668 48-49 0.016581072780410936 50-51 0.010215273984313471 52-53 0.008665485272174561 54-55 0.007998909482007287 56-57 0.008682149666928742 58-59 0.004199427478053825 60-61 0.00721568292856074 62-63 0.011398446011860384 64-65 0.006382463190851647 66-67 0.0034328653193614602 68-69 0.005915860137734556 70-71 0.01326485822432875 72-73 0.01091517856398911 74-75 0.011831720275469111 76-77 0.00568255861117601 78-79 0.00829886858758256 80-81 0.01136511722235202 82-83 0.012781590776457475 84-85 0.007532306428890194 86-87 0.002482994818373095 88-89 0.016031147753522934 90-91 0.025679832316194227 92-93 0.0106485482479222 94-95 0.020863822232235672 96-97 0.019197382756817486 98-99 0.011415110406614564 100-101 0.12536624173571004 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3000409.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.430736551804 #Duplication Level Percentage of deduplicated Percentage of total 1 74.32315471361788 38.968177444886685 2 13.532043164836546 14.1898998036637 3 5.004140662131444 7.8711234217315145 4 2.409975261873385 5.054271122065932 5 1.2993139775576856 3.4061994427701796 6 0.7851844971595267 2.47006809090791 7 0.5080561733046298 1.8646431563236907 8 0.36954273345578337 1.5500318161962965 9 0.2706538408299807 1.277152220276155 >10 1.3658839587130382 12.791906771026365 >50 0.08438103633008208 3.0268855620241957 >100 0.042471960784854663 4.155611994801795 >500 0.0026952693214828045 0.9627249590892107 >1k 0.0023744039256900972 2.005528392845298 >5k 1.2834615814541066E-4 0.4057758013910394 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6794 0.22643579591982294 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5266 0.1755094055510432 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3571 0.11901710733436675 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.332878950836369E-5 0.0 3 0.0 0.0 0.0 3.332878950836369E-5 0.0 4 0.0 0.0 0.0 3.332878950836369E-5 0.0 5 0.0 0.0 0.0 3.332878950836369E-5 0.0 6 0.0 0.0 0.0 3.332878950836369E-5 0.0 7 0.0 0.0 0.0 3.332878950836369E-5 0.0 8 0.0 0.0 0.0 3.332878950836369E-5 0.0 9 0.0 0.0 0.0 3.332878950836369E-5 0.0 10-11 0.0 0.0 0.0 3.332878950836369E-5 0.0 12-13 0.0 0.0 0.0 3.332878950836369E-5 0.0 14-15 0.0 0.0 0.0 4.999318426254554E-5 0.0 16-17 0.0 0.0 0.0 8.332197377090923E-5 0.0 18-19 0.0 0.0 0.0 9.998636852509107E-5 0.0 20-21 0.0 0.0 0.0 1.3331515803345476E-4 0.0 22-23 0.0 0.0 0.0 2.3330152655854584E-4 0.0 24-25 0.0 0.0 0.0 4.999318426254554E-4 0.0 26-27 0.0 0.0 0.0 0.0013331515803345476 0.0 28-29 0.0 0.0 0.0 0.004249420662316371 0.0 30-31 0.0 0.0 0.0 0.013964762804004386 0.0 32-33 0.0 0.0 0.0 0.03412868045656442 0.0 34-35 0.0 0.0 0.0 0.06605766080557685 0.0 36-37 0.0 0.0 0.0 0.11583420793631802 0.0 38-39 0.0 0.0 0.0 0.19110727904095742 0.0 40-41 1.6664394754181846E-5 0.0 0.0 0.2740293073377663 0.0 42-43 3.332878950836369E-5 0.0 0.0 0.3601009062431155 0.0 44-45 3.332878950836369E-5 0.0 0.0 0.46996926085743645 0.0 46-47 3.332878950836369E-5 0.0 0.0 0.5963686950679057 0.0 48-49 3.332878950836369E-5 0.0 0.0 0.7341999040797438 0.0 50-51 3.332878950836369E-5 0.0 0.0 0.8826963257342582 0.0 52-53 3.332878950836369E-5 0.0 0.0 1.0277265532799027 0.0 54-55 3.332878950836369E-5 0.0 0.0 1.1886879422105454 0.0 56-57 3.332878950836369E-5 0.0 0.0 1.3790286590928105 0.0 58-59 3.332878950836369E-5 0.0 0.0 1.5790347249325007 0.0 60-61 3.332878950836369E-5 0.0 0.0 1.7940054172614466 0.0 62-63 3.332878950836369E-5 0.0 3.332878950836369E-5 2.0098093293281014 0.0 64-65 3.332878950836369E-5 0.0 3.332878950836369E-5 2.2412777724636874 0.0 66-67 3.332878950836369E-5 0.0 3.332878950836369E-5 2.5100577954538865 0.0 68-69 3.332878950836369E-5 0.0 3.332878950836369E-5 2.7980018724113944 0.0 70-71 3.332878950836369E-5 0.0 3.332878950836369E-5 3.1047267222568653 0.0 72-73 3.332878950836369E-5 0.0 3.332878950836369E-5 3.4198504270584444 0.0 74-75 3.332878950836369E-5 0.0 3.332878950836369E-5 3.735874009176749 0.0 76-77 3.332878950836369E-5 0.0 3.332878950836369E-5 4.074761140897791 0.0 78-79 3.332878950836369E-5 0.0 3.332878950836369E-5 4.45069322215738 0.0 80-81 3.332878950836369E-5 0.0 3.332878950836369E-5 4.846622577121986 0.0 82-83 3.332878950836369E-5 3.332878950836369E-5 3.332878950836369E-5 5.259849573841433 0.0 84-85 4.999318426254554E-5 3.332878950836369E-5 3.332878950836369E-5 5.674343064562198 0.0 86-87 6.665757901672738E-5 3.332878950836369E-5 3.332878950836369E-5 6.094219154788564 0.0 88-89 6.665757901672738E-5 3.332878950836369E-5 3.332878950836369E-5 6.5366754999068455 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6395 0.0 37.155125 1 GTATCAA 9645 0.0 34.5784 1 ATCAACG 12095 0.0 27.468756 3 TATCAAC 12220 0.0 27.38225 2 TCAACGC 12260 0.0 27.137838 4 CAACGCA 12455 0.0 26.674795 5 AACGCAG 12990 0.0 25.642944 6 GTAAGGT 1365 0.0 24.722557 4 GTACATG 10380 0.0 24.36175 1 TACATGG 10400 0.0 23.856396 2 ACGCAGA 14445 0.0 22.955963 7 TAAGGTG 1615 0.0 22.955658 5 CGCAGAG 14495 0.0 22.745676 8 ACATGGG 10725 0.0 22.380087 3 TCGCGTA 130 2.9420952E-4 21.924116 9 GAGTACT 7995 0.0 20.794191 12-13 AGAGTAC 12795 0.0 20.491983 10-11 GCAGAGT 15820 0.0 20.268028 9 GGTAAGG 1535 0.0 19.507421 3 AGTACTT 8555 0.0 19.210936 12-13 >>END_MODULE