FastQCFastQC Report
Thu 26 May 2016
SRR938476_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938476_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3000409
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71690.23893409198545934No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59440.1981063248377138No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40620.1353815429829733No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68800.035.5661541
GTATCAA96850.032.035511
ATCAACG121650.024.5204323
TATCAAC123750.024.2227042
TCAACGC123000.024.211884
TACATGG98900.024.1127032
CAACGCA124900.023.9196225
GTACATG102600.023.525261
AACGCAG129800.023.1264256
ACATGGG100800.022.2413963
GAGTACT81350.020.25993512-13
TAAGGTG17300.020.0420845
ACGCAGA149500.019.9201417
CGCAGAG149500.019.761298
AGTACTT85600.019.33726912-13
GTACTTT85450.018.9543314-15
AGAGTAC132600.018.8949210-11
GTAAGGT16150.018.8223384
CATGGGA48400.018.5473794
GCAGAGT161500.017.969459
Produced by FastQC (version 0.11.5)