##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938476_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3000409 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.796066469604643 31.0 31.0 34.0 30.0 34.0 2 32.050224152773836 31.0 31.0 34.0 30.0 34.0 3 32.112508328031275 33.0 31.0 34.0 30.0 34.0 4 35.768325918233145 37.0 35.0 37.0 35.0 37.0 5 35.66023498796331 37.0 35.0 37.0 33.0 37.0 6 35.68896973712584 37.0 35.0 37.0 33.0 37.0 7 35.60520082428762 37.0 35.0 37.0 33.0 37.0 8 35.57246795353567 37.0 35.0 37.0 33.0 37.0 9 37.11134148711059 39.0 37.0 39.0 33.0 39.0 10-11 37.042873654891714 39.0 37.0 39.0 33.0 39.0 12-13 36.93048814344978 39.0 37.0 39.0 33.0 39.0 14-15 37.948686162453185 40.0 37.0 41.0 33.0 41.0 16-17 38.03825611774928 40.0 37.0 41.0 33.0 41.0 18-19 38.16640114731025 40.0 37.5 41.0 33.0 41.0 20-21 38.11186224944666 40.0 37.0 41.0 33.0 41.0 22-23 37.99010068294022 40.0 37.0 41.0 33.0 41.0 24-25 37.876414348843774 40.0 37.0 41.0 32.5 41.0 26-27 37.664680215263985 40.0 37.0 41.0 32.0 41.0 28-29 37.52671285814701 39.5 36.5 41.0 32.0 41.0 30-31 37.27934708234777 39.0 36.0 41.0 31.0 41.0 32-33 37.18541438850504 39.0 36.0 41.0 31.0 41.0 34-35 37.06015479889575 39.0 36.0 41.0 31.0 41.0 36-37 36.94479102682334 39.0 36.0 41.0 30.5 41.0 38-39 36.73331035868777 39.0 35.0 40.5 30.0 41.0 40-41 36.516750216387166 39.0 35.0 40.0 30.0 41.0 42-43 36.25836260989752 38.5 35.0 40.0 30.0 41.0 44-45 35.97811665009671 38.0 35.0 40.0 29.0 41.0 46-47 35.58895553906151 38.0 34.5 40.0 27.5 41.0 48-49 35.20285734378213 38.0 34.0 40.0 27.0 41.0 50-51 34.81710376818627 38.0 33.5 40.0 26.0 41.0 52-53 34.22557724630209 37.5 33.0 40.0 24.0 41.0 54-55 33.80663719512906 37.0 32.5 40.0 23.0 41.0 56-57 33.41766072558775 37.0 32.0 39.5 23.0 40.5 58-59 32.88795094268815 36.0 31.0 39.0 21.0 40.0 60-61 32.3955770696595 36.0 31.0 39.0 19.0 40.0 62-63 31.88533213305253 35.0 30.5 38.5 17.5 40.0 64-65 31.233097054434914 35.0 29.5 38.0 12.5 40.0 66-67 30.587573727448493 34.0 28.5 38.0 8.0 40.0 68-69 29.859229691685364 34.0 28.0 37.0 7.0 39.0 70-71 29.085519340863193 33.5 26.5 36.5 3.5 39.0 72-73 28.501867578720102 33.0 26.0 36.0 2.0 39.0 74-75 27.78383380399139 32.5 25.5 35.0 2.0 38.0 76-77 26.64007973579602 31.0 24.5 34.0 2.0 36.0 78-79 27.134564321064225 32.0 25.5 35.0 2.0 37.0 80-81 26.756753329296107 32.0 25.0 35.0 2.0 36.0 82-83 26.073275010173614 31.5 23.0 34.5 2.0 36.0 84-85 25.287715108173586 31.0 20.5 34.0 2.0 35.0 86-87 24.382283215388302 30.5 17.0 34.0 2.0 35.0 88-89 23.40536923466101 30.0 8.5 34.0 2.0 35.0 90-91 22.547404370537485 29.0 2.0 34.0 2.0 35.0 92-93 21.4630028772744 29.0 2.0 34.0 2.0 35.0 94-95 20.289903976424547 27.5 2.0 33.0 2.0 34.5 96-97 18.90690719165287 25.5 2.0 33.0 2.0 34.0 98-99 17.126721723605016 21.0 2.0 32.0 2.0 34.0 100-101 12.851101466500067 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2896042467468156 1101 2 0.30518391882140605 1101 3 0.34707099766514204 1101 4 0.21672528274717706 1101 5 0.14049313613624292 1101 6 0.16924370232445085 1101 7 0.28843015115100457 1101 8 0.28793961657714107 1101 9 0.37208696035722966 1101 10-11 0.30076816034472387 1101 12-13 0.38998787473917673 1101 14-15 0.3975565726483339 1101 16-17 0.42858739173142624 1101 18-19 0.3832062659676083 1101 20-21 0.40259673446087163 1101 22-23 0.4497296427325139 1101 24-25 0.415985650807535 1101 26-27 0.4989156001707258 1101 28-29 0.5394694438798595 1101 30-31 0.5383212031666318 1101 32-33 0.6115117371597236 1101 34-35 0.45099725230337384 1101 36-37 0.5153136597129873 1101 38-39 0.6537740309301086 1101 40-41 0.6177550036778996 1101 42-43 0.6876601664857773 1101 44-45 0.7504243206661982 1101 46-47 0.8349389479830265 1101 48-49 0.8434938387654967 1101 50-51 1.0352303264556681 1101 52-53 1.0558030999815884 1101 54-55 1.229266447164946 1101 56-57 1.1572050244896772 1101 58-59 1.2571477028266926 1101 60-61 1.3375301367966017 1101 62-63 1.2253666687403637 1101 64-65 1.300614253987721 1101 66-67 1.5114623365279165 1101 68-69 1.654064851852187 1101 70-71 1.7208699345211969 1101 72-73 1.8070658867637484 1101 74-75 1.8570365674848546 1101 76-77 1.7556444829314621 1101 78-79 1.7275372167824798 1101 80-81 1.861197568795827 1101 82-83 1.9143456335186144 1101 84-85 2.3312132543246555 1101 86-87 2.2588652389682977 1101 88-89 2.273646453673628 1101 90-91 2.6073046808544937 1101 92-93 2.8756502007594698 1101 94-95 2.696945174807606 1101 96-97 2.6378788381945277 1101 98-99 2.6872955698259737 1101 100-101 2.289321689797289 1102 1 0.349291442735737 1102 2 0.3351541302622465 1102 3 0.3251633711663615 1102 4 0.2340713249001638 1102 5 0.2988464854179611 1102 6 0.23886419994305186 1102 7 0.3512275237711151 1102 8 0.2928441073110619 1102 9 0.437690347659192 1102 10-11 0.39005538337026735 1102 12-13 0.32225819022270485 1102 14-15 0.5690197847433538 1102 16-17 0.46271575048803015 1102 18-19 0.5364379866793527 1102 20-21 0.43092187634276513 1102 22-23 0.46573694986124536 1102 24-25 0.5018437496022301 1102 26-27 0.5247031020113937 1102 28-29 0.40525810443271126 1102 30-31 0.4770711941141883 1102 32-33 0.38505936385163153 1102 34-35 0.5182010504653718 1102 36-37 0.47886564364551276 1102 38-39 0.5116407814424662 1102 40-41 0.5245980440421221 1102 42-43 0.5032170846010402 1102 44-45 0.47324962307800433 1102 46-47 0.4144568392233978 1102 48-49 0.60157881340659 1102 50-51 0.6593997350055147 1102 52-53 0.7910126216989894 1102 54-55 0.8345011105228437 1102 56-57 0.7667146116260426 1102 58-59 0.9976917061290251 1102 60-61 1.0334270283097595 1102 62-63 1.1674301540983478 1102 64-65 1.3067376858035438 1102 66-67 1.3150367825790035 1102 68-69 1.448191689799046 1102 70-71 1.6676228850128076 1102 72-73 1.6664921311120082 1102 74-75 1.6561815344494875 1102 76-77 1.6892919157804691 1102 78-79 1.8499796613755173 1102 80-81 2.0300755250206457 1102 82-83 2.125190817500389 1102 84-85 2.176498985508257 1102 86-87 2.0739141342086462 1102 88-89 1.882682236154384 1102 90-91 2.277768262289026 1102 92-93 2.5099333562224366 1102 94-95 2.405495867258839 1102 96-97 2.602399131348708 1102 98-99 2.563481137582876 1102 100-101 2.1336808736776707 1103 1 0.20771016172722412 1103 2 0.20067513138742044 1103 3 0.18351784397297166 1103 4 0.13802711237192256 1103 5 0.1327232779649421 1103 6 0.18955380083250617 1103 7 0.12284279252173746 1103 8 0.17561387303322107 1103 9 0.16883261113477488 1103 10-11 0.281869106610948 1103 12-13 0.30388012175579604 1103 14-15 0.3987102354715901 1103 16-17 0.3235902886096582 1103 18-19 0.3646461280534865 1103 20-21 0.3042172467255071 1103 22-23 0.40443284525033363 1103 24-25 0.339834970064814 1103 26-27 0.4560428673211234 1103 28-29 0.40375081690547177 1103 30-31 0.34409001429632013 1103 32-33 0.31983769418274477 1103 34-35 0.3589736195517972 1103 36-37 0.3747253662251424 1103 38-39 0.40157504237608777 1103 40-41 0.4557543069124108 1103 42-43 0.471525359405085 1103 44-45 0.5114494984194948 1103 46-47 0.6639078131688407 1103 48-49 0.5796121947018946 1103 50-51 0.6505363754876967 1103 52-53 0.966105549216401 1103 54-55 0.8996154902247682 1103 56-57 1.0486613755875354 1103 58-59 1.2110388800810838 1103 60-61 1.0287497201668145 1103 62-63 1.1579985701859243 1103 64-65 1.4791051460984086 1103 66-67 1.4282288595024824 1103 68-69 1.3256698004964989 1103 70-71 1.4197232298858609 1103 72-73 1.745258244212014 1103 74-75 1.4167624338147498 1103 76-77 1.4867035380663474 1103 78-79 1.4859455005288638 1103 80-81 1.6293796214991296 1103 82-83 1.9467967049138863 1103 84-85 2.028174585260331 1103 86-87 1.9358123127189373 1103 88-89 1.99427402542225 1103 90-91 2.107879914722975 1103 92-93 2.3066945584974228 1103 94-95 1.972123401454244 1103 96-97 1.7925403625867595 1103 98-99 1.6917684539569997 1103 100-101 1.7312013543274265 1104 1 0.20133150256609156 1104 2 0.1953406102110904 1104 3 0.1555793235104943 1104 4 0.1757173618755914 1104 5 0.08664027799058971 1104 6 0.08157391909040967 1104 7 0.22859002694981712 1104 8 0.1762487737840317 1104 9 0.2942004665439839 1104 10-11 0.2687842319191631 1104 12-13 0.2144762232630626 1104 14-15 0.30470277902481513 1104 16-17 0.26538648135962006 1104 18-19 0.3589886037577301 1104 20-21 0.29268414187648517 1104 22-23 0.2569324185219557 1104 24-25 0.17726278578079047 1104 26-27 0.36250139715218666 1104 28-29 0.3079117878449651 1104 30-31 0.293009322597662 1104 32-33 0.44018331473909456 1104 34-35 0.36988787108021626 1104 36-37 0.4396225621671519 1104 38-39 0.4883474954795588 1104 40-41 0.6028970813987087 1104 42-43 0.48372368298725377 1104 44-45 0.4582943570838438 1104 46-47 0.5844680361759274 1104 48-49 0.6568279866614546 1104 50-51 0.6090081248474846 1104 52-53 0.7130391851520699 1104 54-55 0.8138905622799513 1104 56-57 0.8443814204492668 1104 58-59 0.853569823024749 1104 60-61 0.8117678630915606 1104 62-63 0.7782723382347001 1104 64-65 0.9091030349451366 1104 66-67 0.8242220216313889 1104 68-69 1.001485445841407 1104 70-71 1.2799126872905937 1104 72-73 1.377005203001879 1104 74-75 1.2189605874829432 1104 76-77 1.4414954839666265 1104 78-79 1.5611717998348702 1104 80-81 1.7093875552597808 1104 82-83 1.7003972984800093 1104 84-85 2.0114661564059233 1104 86-87 2.0321681911924685 1104 88-89 1.9131291117968487 1104 90-91 2.20593310045993 1104 92-93 2.302558319479779 1104 94-95 2.1994102074570314 1104 96-97 2.3166316643576295 1104 98-99 2.476930733456147 1104 100-101 2.209572028198446 1105 1 0.1661326705554096 1105 2 0.17883249522754596 1105 3 0.06759215098775684 1105 4 0.1062153783722195 1105 5 -0.008761396461899551 1105 6 0.06095461155747728 1105 7 0.10317831219064999 1105 8 0.1476197762906324 1105 9 0.23803535356366012 1105 10-11 0.15138963774844427 1105 12-13 0.20392077907909822 1105 14-15 0.2874413248435417 1105 16-17 0.13657016880190298 1105 18-19 0.198683766724578 1105 20-21 0.1981941685866957 1105 22-23 0.2667665647976136 1105 24-25 0.15193019403466934 1105 26-27 0.21598996631679057 1105 28-29 0.23412475617741535 1105 30-31 0.27445161908119076 1105 32-33 0.3414083986841092 1105 34-35 0.38646012134208263 1105 36-37 0.41354954259814747 1105 38-39 0.37399924797998096 1105 40-41 0.445150593766968 1105 42-43 0.3805400857664907 1105 44-45 0.3901873290588824 1105 46-47 0.5959040888239784 1105 48-49 0.6450086506286397 1105 50-51 0.599864605377455 1105 52-53 0.6490372892352454 1105 54-55 0.7039186911592878 1105 56-57 0.682112526154107 1105 58-59 0.734796821015216 1105 60-61 0.6651486618007993 1105 62-63 0.7841339970164682 1105 64-65 0.9494721084396396 1105 66-67 1.0518308518734791 1105 68-69 1.0849683930245035 1105 70-71 1.1680686141175656 1105 72-73 1.1964841041289986 1105 74-75 1.1731852314926243 1105 76-77 1.2229299914656586 1105 78-79 1.3386037284316998 1105 80-81 1.3709332544650081 1105 82-83 1.7971669989719459 1105 84-85 2.0342118356275023 1105 86-87 2.042480005637991 1105 88-89 1.785807356752084 1105 90-91 1.9645418782133248 1105 92-93 2.114907770214085 1105 94-95 1.9127915458090392 1105 96-97 1.984149322375469 1105 98-99 2.0621227250731344 1105 100-101 1.765420259150373 1106 1 0.02946517250364522 1106 2 0.09522520153092984 1106 3 0.02429889317593137 1106 4 0.09161680800961136 1106 5 0.047738568868311404 1106 6 -0.05426863603969423 1106 7 0.07643641379694799 1106 8 0.06087315866533771 1106 9 -0.020015852168967285 1106 10-11 0.17366839530723865 1106 12-13 0.14180974052731443 1106 14-15 0.16309717901928167 1106 16-17 0.07551469609737893 1106 18-19 0.10303782707485709 1106 20-21 0.06163378467306302 1106 22-23 0.21039534341701227 1106 24-25 0.09290228329852113 1106 26-27 -0.010669035048273656 1106 28-29 0.20140563014599167 1106 30-31 0.3512708776857707 1106 32-33 0.211855400988874 1106 34-35 0.0906300695650728 1106 36-37 0.06045523765505578 1106 38-39 0.24979626513584208 1106 40-41 0.3928006027190136 1106 42-43 0.40609441436783555 1106 44-45 0.3710401852806626 1106 46-47 -0.15473476680375597 1106 48-49 0.13194176069540475 1106 50-51 0.3941509716622704 1106 52-53 0.4439515410041821 1106 54-55 0.43630291733467175 1106 56-57 0.6049384972446816 1106 58-59 0.8537223368448252 1106 60-61 0.7744097915807089 1106 62-63 0.7527251304496332 1106 64-65 0.8629279210116287 1106 66-67 0.8328282345627969 1106 68-69 0.7559397371465231 1106 70-71 0.7982168973418347 1106 72-73 1.0142831103995746 1106 74-75 0.9496260718884209 1106 76-77 0.9002095303884552 1106 78-79 1.0897584395391746 1106 80-81 1.2422655266611784 1106 82-83 1.4577709881715322 1106 84-85 1.4549726771824076 1106 86-87 1.4510787353781538 1106 88-89 1.5950260030984573 1106 90-91 1.9436585022240713 1106 92-93 1.5947345040401082 1106 94-95 1.724710969069264 1106 96-97 1.6085091472257425 1106 98-99 1.5792792017527795 1106 100-101 1.7475581228080497 1107 1 0.05048059061416765 1107 2 0.05297342293473406 1107 3 0.04739759739373284 1107 4 0.02920235013701955 1107 5 -0.002602743275510022 1107 6 -0.05104164822578383 1107 7 0.057169291644427744 1107 8 -0.05644925751533947 1107 9 0.10913700559925132 1107 10-11 0.07151246923074694 1107 12-13 0.1277258982860161 1107 14-15 0.23522725796109967 1107 16-17 0.1609401058810036 1107 18-19 0.05683613681027566 1107 20-21 0.15679310145435466 1107 22-23 0.1376319064080036 1107 24-25 0.18107093497072668 1107 26-27 0.18355301455747508 1107 28-29 0.10588195490609564 1107 30-31 0.11822246228992839 1107 32-33 0.11853545718661707 1107 34-35 0.24113555206927373 1107 36-37 0.32594354498761646 1107 38-39 0.36254513385144094 1107 40-41 0.29536798737284187 1107 42-43 0.3347409080788353 1107 44-45 0.2359198684051691 1107 46-47 0.4432151438571381 1107 48-49 0.43182997977190496 1107 50-51 0.5368382878269813 1107 52-53 0.5192509793793434 1107 54-55 0.5240866073947359 1107 56-57 0.6269733936470843 1107 58-59 0.6305938613220263 1107 60-61 0.6052291371862637 1107 62-63 0.8868766961129921 1107 64-65 0.9640653259109406 1107 66-67 0.929276180367939 1107 68-69 1.172707177917495 1107 70-71 1.04151374445485 1107 72-73 1.192915826696165 1107 74-75 1.226671539370951 1107 76-77 1.2829859488954263 1107 78-79 1.3500483042484923 1107 80-81 1.4548730446461207 1107 82-83 1.5529743826040452 1107 84-85 1.832469600562355 1107 86-87 1.9091823485869384 1107 88-89 2.0534199243117968 1107 90-91 2.178424636281587 1107 92-93 2.08221403311952 1107 94-95 1.8386111964091931 1107 96-97 1.9561958765280103 1107 98-99 2.3013166168781147 1107 100-101 2.2252255637301435 1108 1 0.030148499241192894 1108 2 0.040179425443767514 1108 3 0.04503284152667675 1108 4 0.013187328799844522 1108 5 0.022886848969235984 1108 6 0.07341009933054465 1108 7 0.06501698236201747 1108 8 0.05584091602980834 1108 9 0.014133073835324694 1108 10-11 0.04404709680632379 1108 12-13 0.10112553323986617 1108 14-15 0.11343296371682499 1108 16-17 0.13859964264649705 1108 18-19 0.07076992577049168 1108 20-21 0.06810022296377838 1108 22-23 0.11939466349239325 1108 24-25 0.11903935590036241 1108 26-27 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-0.5248364977738618 2316 58-59 -0.5621414478808973 2316 60-61 -0.33681906729682964 2316 62-63 -0.3833173487001531 2316 64-65 -0.3087702879125338 2316 66-67 -0.3261571379953061 2316 68-69 -0.015505711840997805 2316 70-71 -0.2629087499207685 2316 72-73 -0.15440373557166964 2316 74-75 0.05345674731129435 2316 76-77 0.09545191163494948 2316 78-79 -0.14000991353606906 2316 80-81 -0.44097972683355735 2316 82-83 0.08842496855217519 2316 84-85 0.3306149452543785 2316 86-87 0.3260936797505707 2316 88-89 0.2976360253202053 2316 90-91 0.34454711055913734 2316 92-93 0.22232121408685757 2316 94-95 0.7240500172630995 2316 96-97 0.7810390111286587 2316 98-99 0.6942076230465375 2316 100-101 0.2693367933838733 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 12.0 4 43.0 5 107.0 6 214.0 7 515.0 8 1376.0 9 2294.0 10 3999.0 11 6041.0 12 9007.0 13 12503.0 14 16789.0 15 22406.0 16 27927.0 17 34616.0 18 34691.0 19 33974.0 20 36043.0 21 39412.0 22 43906.0 23 48670.0 24 55518.0 25 63274.0 26 73547.0 27 85720.0 28 100727.0 29 117701.0 30 136714.0 31 158993.0 32 186724.0 33 222564.0 34 268049.0 35 324489.0 36 376628.0 37 338442.0 38 114335.0 39 2435.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.08158553306422 19.41705950849462 15.000518381197198 23.500836577243966 2 15.97917234018511 20.411099952034117 39.42732268706278 24.182405020717994 3 19.472437344268894 23.83674114207476 29.617039799171035 27.073781714485307 4 13.442900617882428 16.88836421967805 36.28755279696868 33.38118236547084 5 13.850257915697211 37.32930186951802 34.302171144571105 14.51826907021366 6 33.03056349984285 36.1306408559633 16.829005645563655 14.009789998630188 7 29.39122632947708 31.971674528372635 20.28560106305507 18.351498079095215 8 26.35174949540196 35.58546037738867 19.32390528221495 18.738884844994413 9 26.21460633862997 16.814280514972026 19.779222475966506 37.191890670431505 10-11 25.096928452087695 26.555412945368452 27.206674156756627 21.140984445787225 12-13 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28.119859504439354 22.678597824041926 38-39 22.448903062691297 26.61617319001386 28.143360585588777 22.791563161706065 40-41 22.904749912960234 26.367240981905226 28.158368542286826 22.569640562847713 42-43 22.575159623426536 26.502130062199512 27.91550544960286 23.00720486477109 44-45 22.58658793909723 26.669558513912694 28.147200246368055 22.59665330062202 46-47 23.00103852542721 26.861013568821306 27.52703965594013 22.61090824981136 48-49 22.71183695289542 26.876102558017923 27.499850853666953 22.912209635419707 50-51 22.874081500222136 26.99243669779687 27.474387658482556 22.659094143498436 52-53 23.057523157676172 26.834658208264273 27.37055181476925 22.7372668192903 54-55 22.670609240273578 26.957591448365875 27.481620005805873 22.890179305554675 56-57 22.744340103079313 27.026557721483215 27.415888105884267 22.813214069553208 58-59 22.766829455584222 26.69182768082618 27.33550659260121 23.20583627098839 60-61 22.501315653965843 27.02124943632685 27.503100410644016 22.974334499063296 62-63 22.47715228157228 27.04572943222074 27.6325494290945 22.844568857112478 64-65 22.258361787825308 27.014703015092962 27.480273496411318 23.24666170067041 66-67 22.326578939255448 26.87643366659529 27.434327342172733 23.362660051976533 68-69 22.695813849005535 26.707870315824838 27.459608053175906 23.136707781993724 70-71 22.59743804714461 26.77523862183341 27.184383182745403 23.442940148276577 72-73 22.606135848553528 26.826406479136118 27.13684842021064 23.43060925209972 74-75 22.424926068412674 27.15516451257145 27.017633262665193 23.402276156350684 76-77 22.525229060438093 26.780982192761055 27.322858316982785 23.370930429818067 78-79 22.49868267959468 26.896883058276387 27.362719549234786 23.241714712894144 80-81 22.51654359994734 27.051648027516283 27.09842504595213 23.333383326584244 82-83 22.56414076447482 26.996305626616152 27.142837383412434 23.296716225496592 84-85 22.419930545533205 27.045328722145136 27.107407109900244 23.427333622421415 86-87 22.5287548258012 27.110978678982857 26.87065102945426 23.489615465761684 88-89 22.39024746292922 27.231570762519375 26.638184994112468 23.739996780438933 90-91 22.349310442344443 27.03120669798396 26.86311051103607 23.756372348635523 92-93 22.33551288005627 26.86196765125386 27.013131106592812 23.78938836209705 94-95 22.227517800085124 26.73743928740334 26.99780396459547 24.037238947916066 96-97 22.25341982600036 26.567015791349746 26.971810671780194 24.207753710869703 98-99 22.055609751870495 26.7847483459755 26.67547990957233 24.484161992581676 100-101 22.338549013758964 26.541244814246063 26.83235527174225 24.287850900252717 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 567.0 1 404.5 2 329.0 3 413.0 4 366.5 5 333.0 6 554.5 7 1223.0 8 1945.5 9 2167.0 10 1837.5 11 1456.0 12 1467.0 13 1639.5 14 1814.0 15 1956.5 16 2136.5 17 2371.5 18 2592.0 19 2923.5 20 3404.0 21 3956.5 22 4712.5 23 5834.0 24 7172.5 25 9157.5 26 12048.5 27 15732.5 28 19502.0 29 23889.5 30 29292.0 31 36081.0 32 45747.0 33 51685.0 34 54489.0 35 60111.0 36 67800.0 37 74108.0 38 78395.5 39 83155.5 40 88530.0 41 95336.5 42 100449.0 43 104760.0 44 108837.0 45 113148.5 46 115750.0 47 118578.5 48 121258.0 49 118604.0 50 115003.5 51 110630.5 52 105851.0 53 103959.0 54 103837.5 55 98463.0 56 89784.0 57 81809.5 58 74856.0 59 66576.0 60 54510.5 61 44894.5 62 37715.5 63 28802.0 64 20771.0 65 15545.5 66 11691.0 67 9005.0 68 6949.5 69 5309.0 70 4103.5 71 2927.5 72 2087.0 73 1681.0 74 1266.0 75 902.5 76 666.5 77 446.5 78 274.0 79 160.0 80 86.0 81 46.0 82 25.5 83 13.5 84 10.0 85 6.5 86 3.0 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022863549602737496 2 0.01199836422301093 3 0.0014997955278763663 4 0.0 5 3.332878950836369E-4 6 0.0 7 0.0 8 3.332878950836369E-5 9 4.33274263608728E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0017830902386974576 26-27 0.008465512535124379 28-29 2.832947108210914E-4 30-31 0.0 32-33 2.999591055752732E-4 34-35 0.009282067878079288 36-37 9.83199290496729E-4 38-39 0.011715069512189839 40-41 0.011448439196122928 42-43 0.0048160100839585536 44-45 6.665757901672738E-4 46-47 3.332878950836369E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.332878950836369E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.3331515803345476E-4 66-67 5.499250268880009E-4 68-69 9.165417114800015E-4 70-71 8.332197377090923E-5 72-73 2.999591055752732E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.3331515803345476E-4 82-83 0.001183172027546911 84-85 0.005665894216421828 86-87 0.004332742636087281 88-89 0.0 90-91 0.0014498023436138206 92-93 4.8326744787127355E-4 94-95 6.665757901672738E-5 96-97 1.6664394754181846E-5 98-99 0.0 100-101 4.9993184262545543E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3000409.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.55172343634885 #Duplication Level Percentage of deduplicated Percentage of total 1 71.02988599613779 34.48623380599871 2 14.962460178437093 14.529064570217212 3 5.660436262299787 8.244718078087784 4 2.7753487090408107 5.389918518431091 5 1.5136767291474147 3.6745806962801204 6 0.9412030175477308 2.741821716326066 7 0.6248883168694305 2.123758331747508 8 0.41337035671728356 1.6055874588897938 9 0.32896879890571334 1.437480192929225 >10 1.5902228431072845 13.881035112335683 >50 0.1034007738761048 3.4690741318517877 >100 0.04924939837045768 4.401067777371042 >500 0.004133171726135707 1.3812630238859693 >1k 0.0026176754261767296 2.195826595327318 >5k 1.3777239085140683E-4 0.43856999032088106 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7169 0.23893409198545934 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5944 0.1981063248377138 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4062 0.1353815429829733 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.332878950836369E-5 0.0 3 0.0 0.0 0.0 3.332878950836369E-5 0.0 4 0.0 0.0 0.0 3.332878950836369E-5 0.0 5 0.0 0.0 0.0 3.332878950836369E-5 0.0 6 0.0 0.0 0.0 3.332878950836369E-5 0.0 7 0.0 0.0 0.0 3.332878950836369E-5 0.0 8 0.0 0.0 0.0 3.332878950836369E-5 0.0 9 0.0 0.0 0.0 3.332878950836369E-5 0.0 10-11 0.0 0.0 0.0 3.332878950836369E-5 0.0 12-13 0.0 0.0 0.0 3.332878950836369E-5 0.0 14-15 0.0 0.0 0.0 3.332878950836369E-5 0.0 16-17 0.0 0.0 0.0 4.999318426254554E-5 0.0 18-19 0.0 0.0 0.0 9.998636852509107E-5 0.0 20-21 0.0 0.0 0.0 1.3331515803345476E-4 0.0 22-23 0.0 0.0 0.0 2.3330152655854584E-4 0.0 24-25 0.0 0.0 0.0 4.999318426254554E-4 0.0 26-27 0.0 0.0 0.0 0.001316487185580366 0.0 28-29 0.0 0.0 0.0 0.004232756267562189 3.332878950836369E-5 30-31 0.0 0.0 0.0 0.013848112040725113 3.332878950836369E-5 32-33 0.0 0.0 0.0 0.03394537211426842 3.332878950836369E-5 34-35 0.0 0.0 0.0 0.0663576199111521 3.332878950836369E-5 36-37 0.0 0.0 0.0 0.11656744130550202 3.332878950836369E-5 38-39 0.0 0.0 0.0 0.19210714272620832 3.332878950836369E-5 40-41 0.0 0.0 0.0 0.27564575362892196 3.332878950836369E-5 42-43 0.0 0.0 0.0 0.3622006199821425 3.332878950836369E-5 44-45 0.0 0.0 0.0 0.4722356185440052 3.332878950836369E-5 46-47 0.0 0.0 0.0 0.5991516489918541 3.332878950836369E-5 48-49 0.0 0.0 0.0 0.737616105004351 3.332878950836369E-5 50-51 0.0 0.0 0.0 0.8867957668437869 3.332878950836369E-5 52-53 0.0 0.0 0.0 1.0322925974425488 3.332878950836369E-5 54-55 0.0 0.0 0.0 1.1939038977686043 3.332878950836369E-5 56-57 0.0 0.0 0.0 1.384861197256774 3.332878950836369E-5 58-59 0.0 0.0 0.0 1.5855005100971233 3.332878950836369E-5 60-61 0.0 0.0 0.0 1.8012544289795158 3.332878950836369E-5 62-63 0.0 0.0 0.0 2.017708252441584 3.332878950836369E-5 64-65 0.0 0.0 0.0 2.249926593341108 3.332878950836369E-5 66-67 0.0 0.0 0.0 2.5208896520441044 3.332878950836369E-5 68-69 0.0 0.0 0.0 2.8115666897412988 3.332878950836369E-5 70-71 0.0 0.0 0.0 3.1230242276969573 6.665757901672738E-5 72-73 0.0 0.0 0.0 3.445280293453326 9.998636852509107E-5 74-75 0.0 0.0 0.0 3.770819244976268 9.998636852509107E-5 76-77 0.0 0.0 0.0 4.1211548158934335 9.998636852509107E-5 78-79 0.0 0.0 0.0 4.513134709301299 9.998636852509107E-5 80-81 0.0 0.0 0.0 4.930594462288308 9.998636852509107E-5 82-83 0.0 0.0 0.0 5.367668207900989 9.998636852509107E-5 84-85 0.0 0.0 0.0 5.810691142440914 9.998636852509107E-5 86-87 0.0 0.0 0.0 6.2629461516746545 9.998636852509107E-5 88-89 0.0 0.0 0.0 6.748296648890202 9.998636852509107E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6880 0.0 35.566154 1 GTATCAA 9685 0.0 32.03551 1 ATCAACG 12165 0.0 24.520432 3 TATCAAC 12375 0.0 24.222704 2 TCAACGC 12300 0.0 24.21188 4 TACATGG 9890 0.0 24.112703 2 CAACGCA 12490 0.0 23.919622 5 GTACATG 10260 0.0 23.52526 1 AACGCAG 12980 0.0 23.126425 6 ACATGGG 10080 0.0 22.241396 3 GAGTACT 8135 0.0 20.259935 12-13 TAAGGTG 1730 0.0 20.042084 5 ACGCAGA 14950 0.0 19.920141 7 CGCAGAG 14950 0.0 19.76129 8 AGTACTT 8560 0.0 19.337269 12-13 GTACTTT 8545 0.0 18.95433 14-15 AGAGTAC 13260 0.0 18.89492 10-11 GTAAGGT 1615 0.0 18.822338 4 CATGGGA 4840 0.0 18.547379 4 GCAGAGT 16150 0.0 17.96945 9 >>END_MODULE