FastQCFastQC Report
Thu 26 May 2016
SRR938475_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938475_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3073074
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67440.2194545266401004No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA57830.18818290740802207No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA56700.18450580753994208No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53520.1741578627784427No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA52170.16976486736082502No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA48450.15765972443227858No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC40780.13270100231885076No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG40720.13250575807806775No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA40100.13048823425664333No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA39880.12977233870710567No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA37270.12127921423304483No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36630.11919660899802609No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG35650.11600761973190363No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT31340.10198257510232425No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70000.033.2260251
GTATCAA110850.032.437971
TAAGGTG21300.028.5814195
TATCAAC132150.026.5968782
ATCAACG132150.026.5968783
TCAACGC132600.026.470754
CAACGCA133750.026.1720315
AACGCAG139650.025.0786916
AGGTAAG21750.024.7099912
GTAAGGT23350.024.6463094
GGTAAGG24150.023.4359863
TACATGG89700.022.693672
GTACATG91950.022.656351
AAGGTAA22400.022.5074041
ACGCAGA155450.022.2560067
CGCAGAG157850.021.8574078
ACATGGG89750.021.6211663
GAGTACT87600.021.18212-13
TAAGGTA11500.020.6788124
AGAGTAC126900.020.40909810-11
Produced by FastQC (version 0.11.5)