##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938475_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3073074 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.89143736857622 31.0 31.0 34.0 27.0 34.0 2 30.94246900660381 31.0 31.0 34.0 27.0 34.0 3 30.97614863813888 31.0 31.0 34.0 27.0 34.0 4 34.63225128975091 37.0 35.0 37.0 32.0 37.0 5 34.60691834950932 37.0 35.0 37.0 30.0 37.0 6 34.613824138305816 37.0 35.0 37.0 30.0 37.0 7 34.515248249798084 37.0 35.0 37.0 30.0 37.0 8 34.46906712952568 37.0 35.0 37.0 30.0 37.0 9 35.93849546089681 39.0 35.0 39.0 30.0 39.0 10-11 35.76026382703443 38.0 35.0 39.0 30.0 39.0 12-13 35.61710261451563 38.0 35.0 39.0 30.0 39.0 14-15 36.546644011826594 39.0 36.0 41.0 30.0 41.0 16-17 36.36314615267969 39.0 36.0 40.0 29.0 41.0 18-19 36.507218830395885 39.0 36.0 41.0 30.0 41.0 20-21 36.47415698417936 39.0 36.0 41.0 29.0 41.0 22-23 36.38852611424261 39.0 36.0 41.0 29.0 41.0 24-25 36.23579191389469 39.0 35.0 41.0 28.0 41.0 26-27 36.018262332765175 39.0 35.0 40.5 27.0 41.0 28-29 35.80759103100023 38.5 35.0 40.0 27.0 41.0 30-31 35.536355453854995 38.0 34.0 40.0 27.0 41.0 32-33 35.24935813455843 38.0 34.0 40.0 25.5 41.0 34-35 34.98647640766217 38.0 34.0 40.0 25.0 41.0 36-37 34.69088346066512 38.0 33.5 40.0 24.5 41.0 38-39 34.52943697418286 38.0 33.0 40.0 24.0 41.0 40-41 34.41904913451482 38.0 33.0 40.0 24.0 41.0 42-43 34.11767468014112 38.0 33.0 40.0 23.0 41.0 44-45 33.774050185579654 38.0 33.0 40.0 21.5 41.0 46-47 33.38665079330989 38.0 32.0 40.0 18.5 41.0 48-49 33.022172912204525 37.0 31.5 40.0 16.5 41.0 50-51 32.325861987052704 36.5 30.5 39.5 15.0 40.5 52-53 32.358297912773985 37.0 31.0 39.0 14.5 40.0 54-55 32.45431837957693 37.0 31.0 40.0 12.5 41.0 56-57 32.1308666826767 36.5 31.0 39.5 8.5 41.0 58-59 31.749090649948556 36.0 30.5 39.0 7.5 41.0 60-61 31.261609222556956 35.5 29.5 39.0 4.5 40.0 62-63 30.692432072901596 35.0 28.5 39.0 2.0 40.0 64-65 30.17573755138991 35.0 28.0 38.0 2.0 40.0 66-67 29.632383893131113 34.0 28.0 38.0 2.0 40.0 68-69 29.050219421985933 34.0 26.5 37.5 2.0 39.5 70-71 28.39223575481749 34.0 26.0 36.5 2.0 39.0 72-73 27.758784363799897 33.0 25.5 36.0 2.0 39.0 74-75 27.088375027740952 33.0 23.5 35.5 2.0 38.5 76-77 26.332586849519405 32.0 21.5 35.0 2.0 37.5 78-79 25.540981928843888 31.5 19.5 35.0 2.0 37.0 80-81 24.843247347769694 31.0 17.0 35.0 2.0 36.0 82-83 24.093464068876962 31.0 9.5 34.0 2.0 36.0 84-85 23.38623931607244 30.0 4.5 34.0 2.0 35.0 86-87 22.63078646983444 29.5 2.0 34.0 2.0 35.0 88-89 21.79159271791047 29.0 2.0 34.0 2.0 35.0 90-91 20.87535851723714 28.5 2.0 34.0 2.0 35.0 92-93 19.933223052878 26.5 2.0 33.0 2.0 35.0 94-95 18.928685739425735 25.5 2.0 33.0 2.0 34.5 96-97 17.686951404359284 22.0 2.0 32.0 2.0 34.0 98-99 16.188761643878408 10.0 2.0 32.0 2.0 34.0 100-101 12.832222719010346 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5397164380196102 1101 2 0.3332702654347557 1101 3 0.3629423857602241 1101 4 0.3183260550755236 1101 5 0.342710023886049 1101 6 0.5037293389702455 1101 7 0.4796417208472974 1101 8 0.540730243383031 1101 9 0.7059690992650829 1101 10-11 0.7298390038224838 1101 12-13 0.7510042244922346 1101 14-15 0.984454070127839 1101 16-17 0.7379233285980504 1101 18-19 0.7793576576862833 1101 20-21 0.8663626392248673 1101 22-23 0.954597084840465 1101 24-25 0.8537908995059738 1101 26-27 0.8161649183311326 1101 28-29 1.078059502931815 1101 30-31 0.8351571402386355 1101 32-33 0.8976861505049243 1101 34-35 0.7610089752011717 1101 36-37 0.760232269478017 1101 38-39 0.8632605662646498 1101 40-41 0.867660147089822 1101 42-43 0.9653414740962987 1101 44-45 1.033879201951187 1101 46-47 0.9422204141836659 1101 48-49 0.7804145171018249 1101 50-51 0.8600851738002433 1101 52-53 0.8683511596275011 1101 54-55 0.9305804117061811 1101 56-57 1.0061832079769601 1101 58-59 0.9658635920171719 1101 60-61 1.0564202284065694 1101 62-63 0.916175717902842 1101 64-65 0.8877100302648167 1101 66-67 0.9325559186075445 1101 68-69 0.883807363386456 1101 70-71 0.8594210306580301 1101 72-73 0.9406983524938681 1101 74-75 0.9221958235008856 1101 76-77 1.1600357460659367 1101 78-79 1.230068019506664 1101 80-81 1.0607494040714371 1101 82-83 1.0867321369595615 1101 84-85 1.0817420077845732 1101 86-87 1.1558726480401127 1101 88-89 1.120824387866314 1101 90-91 1.1567693975319457 1101 92-93 1.077749322464598 1101 94-95 0.9268209172202226 1101 96-97 0.8851199992774532 1101 98-99 0.9128764756547021 1101 100-101 0.9359946825444414 1102 1 0.4808801229981192 1102 2 0.4463949408072807 1102 3 0.4777562604252523 1102 4 0.4116256961341307 1102 5 0.523208881364809 1102 6 0.5129623365052893 1102 7 0.49629733831717004 1102 8 0.6771137371988729 1102 9 0.7558026914231633 1102 10-11 0.8719523893960641 1102 12-13 0.8718428023062899 1102 14-15 0.8587546033363154 1102 16-17 0.9525445859767174 1102 18-19 0.8413177532987177 1102 20-21 0.8734037807556305 1102 22-23 0.966405629813174 1102 24-25 0.8467066936210372 1102 26-27 0.8536329400575795 1102 28-29 0.8761462972236842 1102 30-31 0.8505031228487709 1102 32-33 0.8786280803765365 1102 34-35 0.9187287631044398 1102 36-37 1.0143002958458496 1102 38-39 0.7534642271653951 1102 40-41 0.946403797190996 1102 42-43 1.1248966408498475 1102 44-45 1.0884641680415896 1102 46-47 0.9173248512236967 1102 48-49 1.0713983246654166 1102 50-51 0.987290397697798 1102 52-53 0.8524376845279065 1102 54-55 0.8599432075971691 1102 56-57 1.0049132282100288 1102 58-59 1.0648659703657515 1102 60-61 0.8830704724020286 1102 62-63 1.030174061360686 1102 64-65 1.2394171491135815 1102 66-67 0.9117226401015124 1102 68-69 1.0929153068219044 1102 70-71 1.3482841101756442 1102 72-73 1.3228170995795452 1102 74-75 1.2273766925921876 1102 76-77 1.2576562969499427 1102 78-79 1.515836135663669 1102 80-81 1.502659298609224 1102 82-83 1.514496049078609 1102 84-85 1.2915253438413252 1102 86-87 1.5229450088351868 1102 88-89 1.401939579989559 1102 90-91 1.39723082251707 1102 92-93 1.640126304950087 1102 94-95 1.7125258125128369 1102 96-97 1.5969914182072493 1102 98-99 1.799888529606683 1102 100-101 1.4769328538549207 1103 1 0.5320816374419053 1103 2 0.4769895088451577 1103 3 0.5033029108005067 1103 4 0.3193100555113517 1103 5 0.365166206815438 1103 6 0.5064553603748578 1103 7 0.6160720894599194 1103 8 0.49635143793641134 1103 9 0.6990489469782446 1103 10-11 0.7733117268908316 1103 12-13 0.7687140111171686 1103 14-15 0.8189412103165168 1103 16-17 0.8708825149675263 1103 18-19 0.8638258590522625 1103 20-21 0.8309755659299825 1103 22-23 0.9502434352942615 1103 24-25 0.9810972564737881 1103 26-27 1.0207794910771355 1103 28-29 1.0030116707138887 1103 30-31 1.0150827948380012 1103 32-33 1.0253271517934621 1103 34-35 1.0221126783489183 1103 36-37 1.0834691644556997 1103 38-39 1.0676322076924123 1103 40-41 1.0958209956800218 1103 42-43 1.0016851917497789 1103 44-45 1.0907419064758486 1103 46-47 0.866957883522069 1103 48-49 0.923422034879188 1103 50-51 1.1318327291138814 1103 52-53 1.149453908819737 1103 54-55 1.1994646976038368 1103 56-57 1.1941311491281894 1103 58-59 1.0557404054971187 1103 60-61 1.2704796037200232 1103 62-63 1.3367637730237014 1103 64-65 1.397321132062391 1103 66-67 1.335749892601502 1103 68-69 1.534995868035466 1103 70-71 1.361962983072953 1103 72-73 1.3962368942840158 1103 74-75 1.392452344008941 1103 76-77 1.4350126044612956 1103 78-79 1.4079628360911798 1103 80-81 1.4682280528869782 1103 82-83 1.4041921649375766 1103 84-85 1.4774158078406394 1103 86-87 1.6845694653125065 1103 88-89 1.551700140070757 1103 90-91 1.3448985434682008 1103 92-93 1.4186270293425949 1103 94-95 1.3808460483364975 1103 96-97 1.683708484145921 1103 98-99 1.7690594985667012 1103 100-101 1.5388092838623493 1104 1 0.4129495622165358 1104 2 0.4418271102151081 1104 3 0.3951067138463351 1104 4 0.4112396258354991 1104 5 0.520134123104036 1104 6 0.5103306462343085 1104 7 0.5041302905526592 1104 8 0.5322912872332708 1104 9 0.573236192261426 1104 10-11 0.6721557776416276 1104 12-13 0.7705373102670379 1104 14-15 0.9220187008023757 1104 16-17 0.9770921111414168 1104 18-19 0.9749738880091954 1104 20-21 1.0519421462132428 1104 22-23 1.0019866611596697 1104 24-25 0.8810361135582099 1104 26-27 1.0172317876355095 1104 28-29 0.902876655841581 1104 30-31 1.0100270808040719 1104 32-33 0.9887605332142897 1104 34-35 0.8741537855361443 1104 36-37 0.900270737199456 1104 38-39 1.1266828823315933 1104 40-41 1.12853223792564 1104 42-43 1.1672951304110342 1104 44-45 0.9694663477579795 1104 46-47 0.9648099394254004 1104 48-49 0.7673175348911556 1104 50-51 0.8051549435753813 1104 52-53 0.9065686812527645 1104 54-55 1.0157581966540548 1104 56-57 1.0929792253022583 1104 58-59 1.092139588227397 1104 60-61 1.1304647205334568 1104 62-63 1.1971019303372437 1104 64-65 1.3110884060239947 1104 66-67 1.2128982174814489 1104 68-69 1.338138744450621 1104 70-71 1.6832115605754048 1104 72-73 1.6452252075011131 1104 74-75 1.5743783023316666 1104 76-77 1.5445248972335932 1104 78-79 1.5646478673915638 1104 80-81 1.6534135161044006 1104 82-83 1.795268345528946 1104 84-85 1.6672490030040414 1104 86-87 1.774861667523659 1104 88-89 1.7207897851589813 1104 90-91 1.5208736206584135 1104 92-93 1.656804252409792 1104 94-95 1.7794203957283514 1104 96-97 1.9558669161021207 1104 98-99 2.0493543988994 1104 100-101 1.930071177900155 1105 1 0.5348455760849724 1105 2 0.49587487115366713 1105 3 0.4715502032065295 1105 4 0.5177679560395276 1105 5 0.47792713398858666 1105 6 0.6352979007370081 1105 7 0.6331128996930104 1105 8 0.610386193338492 1105 9 0.7889908729106381 1105 10-11 0.8118360145990025 1105 12-13 0.76952509606609 1105 14-15 0.8607394816428879 1105 16-17 0.9432490787048451 1105 18-19 0.9973546917898304 1105 20-21 1.0595909725415638 1105 22-23 1.0228591890703171 1105 24-25 1.0855853633079846 1105 26-27 1.0509498392497179 1105 28-29 0.8619884622762939 1105 30-31 1.0736454504279394 1105 32-33 1.2128621063549403 1105 34-35 1.184165610444019 1105 36-37 1.1990961783895813 1105 38-39 1.2027178226904311 1105 40-41 1.4635539517127683 1105 42-43 1.3306374463604627 1105 44-45 1.3466478762708292 1105 46-47 1.2869356153394307 1105 48-49 1.2871757090544165 1105 50-51 1.43675818399673 1105 52-53 1.4864915427761005 1105 54-55 1.4796556466278048 1105 56-57 1.411954706100083 1105 58-59 1.511834426284608 1105 60-61 1.38681207641935 1105 62-63 1.562188081000972 1105 64-65 1.7970041893015107 1105 66-67 1.904877788465857 1105 68-69 1.7935810721936498 1105 70-71 1.9307314609664736 1105 72-73 1.8899132355385646 1105 74-75 2.061475480530838 1105 76-77 2.0371949676925105 1105 78-79 1.8496626406987957 1105 80-81 2.1712351029798995 1105 82-83 2.161374798922843 1105 84-85 2.1242756387916586 1105 86-87 2.2889853353950826 1105 88-89 2.35813389832742 1105 90-91 2.243376691690308 1105 92-93 2.2769653550380937 1105 94-95 2.352890907038205 1105 96-97 2.320227864891816 1105 98-99 2.297688474985847 1105 100-101 1.9906954871136673 1106 1 0.27539075810807034 1106 2 0.25910401266689576 1106 3 0.19117590865525003 1106 4 0.12280161671328926 1106 5 0.41343053372337124 1106 6 0.29592826026451746 1106 7 0.5061827342865897 1106 8 0.4401089821227515 1106 9 0.23311028011979573 1106 10-11 0.23685067028036144 1106 12-13 0.41421937684377497 1106 14-15 0.6379375929945752 1106 16-17 0.3857007061510629 1106 18-19 0.27045806948023454 1106 20-21 0.3881095558626484 1106 22-23 0.47825370737414374 1106 24-25 0.22174003572774126 1106 26-27 0.2981088021402556 1106 28-29 0.5015715126739124 1106 30-31 0.17635279685302407 1106 32-33 0.30985676711851795 1106 34-35 -0.07274985629035058 1106 36-37 0.2127764372619012 1106 38-39 0.3445468517234431 1106 40-41 0.45668222670981606 1106 42-43 0.25220984273295954 1106 44-45 0.42750709543767584 1106 46-47 0.5350865754354714 1106 48-49 0.8839596636446387 1106 50-51 0.5405243444817032 1106 52-53 0.46425810499093956 1106 54-55 0.5385988339657999 1106 56-57 -0.1345997041075364 1106 58-59 0.337960033890667 1106 60-61 1.0718020999896432 1106 62-63 0.8098484810754307 1106 64-65 0.9526318828883227 1106 66-67 0.6835521897272763 1106 68-69 0.5948475468214234 1106 70-71 0.5058833812569716 1106 72-73 0.9962293136523357 1106 74-75 1.1477114882268964 1106 76-77 1.3135310380306642 1106 78-79 1.4296194697594231 1106 80-81 1.4189738704192294 1106 82-83 1.2123659000573497 1106 84-85 1.0591474897625197 1106 86-87 0.8217787367490104 1106 88-89 1.0018163440273327 1106 90-91 1.158546531328824 1106 92-93 1.1780953678948975 1106 94-95 1.132329454852087 1106 96-97 1.1118701744842419 1106 98-99 1.1672529109329837 1106 100-101 0.6352865902371434 1107 1 0.20445153060246213 1107 2 0.174130621335987 1107 3 0.30665624323223994 1107 4 0.17614540309813975 1107 5 0.2673534032942797 1107 6 0.2953019697490262 1107 7 0.2849939823254033 1107 8 0.17125133999000752 1107 9 0.39924586433033227 1107 10-11 0.40531458741761384 1107 12-13 0.43768375018630934 1107 14-15 0.4725662518105409 1107 16-17 0.6408892974486449 1107 18-19 0.5051711262738223 1107 20-21 0.6040694018636117 1107 22-23 0.5769247119580214 1107 24-25 0.5640245630296405 1107 26-27 0.5883040084144682 1107 28-29 0.648655617093354 1107 30-31 0.7283270748089805 1107 32-33 0.7715976068139341 1107 34-35 0.6608187202896474 1107 36-37 0.6844761887291995 1107 38-39 0.7405615018635743 1107 40-41 0.6197419459650462 1107 42-43 0.766479299392465 1107 44-45 0.7997585081864358 1107 46-47 0.813022405203462 1107 48-49 0.7822892580781442 1107 50-51 0.6182122365950988 1107 52-53 0.7750515253206345 1107 54-55 0.9546253256083119 1107 56-57 0.9424816333276738 1107 58-59 0.8583812700483122 1107 60-61 0.8419642908857732 1107 62-63 0.9666973620832131 1107 64-65 1.0137871930186613 1107 66-67 0.9810593253729749 1107 68-69 1.184618630943696 1107 70-71 1.2968758558532762 1107 72-73 1.3977336505777913 1107 74-75 1.2897052342474993 1107 76-77 1.383313090914772 1107 78-79 1.5166532229999667 1107 80-81 1.6368071333382694 1107 82-83 1.4508527663039104 1107 84-85 1.7255776734942394 1107 86-87 1.8720509722937493 1107 88-89 1.7466750592342386 1107 90-91 1.578825600035323 1107 92-93 1.7449964348202158 1107 94-95 1.7371900246326497 1107 96-97 1.8385681011303987 1107 98-99 1.659556033558978 1107 100-101 1.603489974486953 1108 1 0.3903677749342833 1108 2 0.276064470362396 1108 3 0.2522131536464478 1108 4 0.32384002642626797 1108 5 0.2195787586271365 1108 6 0.34401972305467154 1108 7 0.43821388858611954 1108 8 0.6053404304141239 1108 9 0.48866399487943113 1108 10-11 0.6169329237358667 1108 12-13 0.49901820127802665 1108 14-15 0.569285376910571 1108 16-17 0.6577423630280421 1108 18-19 0.630737944091905 1108 20-21 0.5110141109288904 1108 22-23 0.5141470188589068 1108 24-25 0.6380585911514274 1108 26-27 0.6883078189544207 1108 28-29 0.7195992973128398 1108 30-31 0.6977170073624279 1108 32-33 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-2.5672028631948294 2315 88-89 -2.7268016197265297 2315 90-91 -2.753687698439542 2315 92-93 -2.7441099167307463 2315 94-95 -3.126937169502428 2315 96-97 -3.1856500754153494 2315 98-99 -3.1122353412359924 2315 100-101 -2.8138007614375056 2316 1 -0.9728320815054232 2316 2 -0.9394451686860066 2316 3 -0.843281177186789 2316 4 -0.9519510200989885 2316 5 -0.8458678896794396 2316 6 -0.8057224543741341 2316 7 -1.01389007543591 2316 8 -0.9543473259435515 2316 9 -1.2145386448721212 2316 10-11 -1.0962940333718265 2316 12-13 -1.266543807980149 2316 14-15 -1.4020615262887688 2316 16-17 -1.4459416798056637 2316 18-19 -1.3521027552518134 2316 20-21 -1.2392662344947567 2316 22-23 -1.2440877388464742 2316 24-25 -1.42787861586838 2316 26-27 -1.3549567494857513 2316 28-29 -1.524545310386678 2316 30-31 -1.485775939139394 2316 32-33 -1.4793676741792368 2316 34-35 -1.5192192977101158 2316 36-37 -1.6159079703169894 2316 38-39 -2.0141055602964 2316 40-41 -1.8390235361509326 2316 42-43 -1.6615737160028274 2316 44-45 -1.6879391781777286 2316 46-47 -1.8936455757549808 2316 48-49 -2.062771657537091 2316 50-51 -2.0344777810574044 2316 52-53 -2.0687946953067566 2316 54-55 -2.3108793986074545 2316 56-57 -2.1572043835724273 2316 58-59 -2.0328554182258003 2316 60-61 -2.1841605137791227 2316 62-63 -2.3043275684643056 2316 64-65 -2.495333871340641 2316 66-67 -2.516683649797759 2316 68-69 -2.5947903356612336 2316 70-71 -3.14422500217427 2316 72-73 -2.975004802946927 2316 74-75 -3.0096507024928663 2316 76-77 -2.9307097994660545 2316 78-79 -3.135971140352275 2316 80-81 -3.265279154137133 2316 82-83 -3.359755738731394 2316 84-85 -3.2584620012366337 2316 86-87 -3.2000490248123974 2316 88-89 -3.6425616145705995 2316 90-91 -3.8072477302112304 2316 92-93 -3.6524681878212952 2316 94-95 -3.9484192038660186 2316 96-97 -4.014135119728497 2316 98-99 -4.0031982638134025 2316 100-101 -3.4712443335926846 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13070.0 3 4791.0 4 5038.0 5 5761.0 6 6822.0 7 8263.0 8 10590.0 9 13114.0 10 16142.0 11 19954.0 12 23721.0 13 26916.0 14 30650.0 15 34299.0 16 38766.0 17 43053.0 18 41873.0 19 41300.0 20 43769.0 21 47685.0 22 51891.0 23 57661.0 24 64516.0 25 71238.0 26 79507.0 27 89181.0 28 100270.0 29 113877.0 30 129797.0 31 149185.0 32 174502.0 33 205819.0 34 244613.0 35 290792.0 36 329596.0 37 305389.0 38 133304.0 39 6358.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.22429016938827 18.450633135484257 14.45298402378745 23.872092671340024 2 15.350936202331896 19.724962365894395 40.18902240585284 24.735079025920868 3 19.27618206122899 23.245702772855477 29.82237682541948 27.655738340496054 4 12.805887266931398 16.21165046519235 36.84891641915923 34.13354584871702 5 13.652543701509993 37.24827894390829 34.46199498114262 14.637182373439103 6 32.91244752092241 35.88834387122223 17.022325167188757 14.176883440666598 7 28.895506686279187 32.54486136423336 20.32141923652434 18.23821271296311 8 26.603626250688023 34.6766447204601 19.711940170737083 19.007788858114797 9 26.324027743285107 16.68447627860591 19.97288034967814 37.018615628430844 10-11 25.01662913241673 26.2138778290281 27.50061290928584 21.268880129269323 12-13 26.134709232768603 23.556639697513976 27.41601938041222 22.8926316893052 14-15 22.4090197051751 25.23496071578245 25.737017491750517 26.619002087291932 16-17 22.251180653301336 28.27969015907407 26.93069529314186 22.538433894482733 18-19 22.37283975373974 26.76024586961957 28.914355806491937 21.952558570148756 20-21 22.62397610825653 26.094507774112706 29.17660650045186 22.104909617178897 22-23 22.302031813449982 26.07379996445352 29.140986069815632 22.483182152280868 24-25 22.29434622015084 26.284142686680873 29.19216600439201 22.229345088776277 26-27 21.74716145197818 26.648659851079913 28.876860829543205 22.727317867398707 28-29 21.994741392656735 27.255839500961386 28.629699914907004 22.119719191474875 30-31 22.093966091318652 26.536530881062358 28.543909837360122 22.825593190258868 32-33 21.580034202059124 26.60311367341673 28.628637428057246 23.1882146964669 34-35 22.677042515211447 26.79893339133753 28.327274586280488 22.19674950717053 36-37 22.894133516661785 26.06480300466781 28.379254169455205 22.6618093092152 38-39 22.061408505720937 26.641802705034095 28.4056900115558 22.891098777689166 40-41 22.73051402937799 26.41408333557139 28.52049614931682 22.334906485733793 42-43 22.356029216763588 26.482326598911754 28.23064817471725 22.93099600960741 44-45 22.35746620839376 26.712930035603854 28.556484283357932 22.373119472644458 46-47 22.9109562960152 27.064520706392514 27.62581583310172 22.39870716449057 48-49 22.388457999318206 27.092719860126547 27.667867263412337 22.85095487714291 50-51 22.646390673772245 27.291101119127152 27.66860740696626 22.39390080013434 52-53 23.070601995739317 26.79754525756441 27.752928488068978 22.3789242586273 54-55 22.226952037234927 27.145775231054447 28.043485441906018 22.583787289804608 56-57 22.48255876774228 27.289540077406656 27.644190400064574 22.583710754786495 58-59 22.459407361720583 26.751018528388197 27.65480939869599 23.134764711195228 60-61 21.83933654873323 27.08249655469619 28.21581760623481 22.862349290335768 62-63 22.168615844044982 27.030193088731387 28.3307077978323 22.470483269391334 64-65 21.77812570731948 27.21455372422469 27.97638816049503 23.0309324079608 66-67 21.744925424160712 27.179334305211512 27.957059234830623 23.118681035797152 68-69 22.65092526795206 26.65633808913288 28.046302532508417 22.646434110406645 70-71 22.25792706685099 26.822978801803714 27.59406347642867 23.32503065491662 72-73 22.198156745028115 26.835784691882687 27.869352452042108 23.096706111047098 74-75 22.23072880530303 27.312479076002795 27.640069326482063 22.816722792212115 76-77 22.30540703062891 26.776604496406243 27.80716993629454 23.110818536670312 78-79 22.412370208067365 27.080811962905386 27.64596233068513 22.86085549834212 80-81 22.750288366680383 27.020621004093677 27.271995067868072 22.957095561357875 82-83 22.45109320684204 27.118741144318403 27.59739541265117 22.83277023618839 84-85 22.370779105189666 27.232217676023634 27.565795404141436 22.831207814645268 86-87 22.6875092245128 27.210469901360568 27.145444713863952 22.956576160262678 88-89 22.449847242635986 27.27920986111043 26.92838064172742 23.342562254526158 90-91 22.28109064813448 27.25336121603566 27.13545436537744 23.33009377045242 92-93 22.585760893872205 26.702335881087862 27.476150810662485 23.235752414377448 94-95 22.23545475677784 26.456526566031396 27.759303596811257 23.54871508037951 96-97 22.598545972710983 25.988129279932075 27.743767204576137 23.669557542780804 98-99 22.315298971716384 26.338157747622226 27.234355996758737 24.11218728390266 100-101 22.782272246480268 25.64242931877156 27.763517491845473 23.811780942902697 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 190.0 1 142.0 2 140.0 3 189.5 4 182.0 5 196.5 6 332.0 7 680.0 8 1121.5 9 1341.5 10 1237.0 11 1128.5 12 1223.5 13 1397.0 14 1568.5 15 1762.0 16 1984.5 17 2274.0 18 2659.5 19 3165.5 20 3807.0 21 4613.0 22 5725.5 23 7158.5 24 9276.0 25 12120.0 26 15806.5 27 20554.0 28 25024.5 29 29584.0 30 34856.5 31 43650.5 32 56231.5 33 60836.5 34 60144.5 35 64058.5 36 70435.5 37 76860.5 38 81836.0 39 85998.5 40 91840.0 41 98401.5 42 102175.5 43 104575.5 44 106727.5 45 112135.5 46 115525.0 47 117602.0 48 120327.0 49 117318.5 50 112774.0 51 108371.5 52 104722.5 53 104447.5 54 104706.0 55 98355.0 56 89734.5 57 82328.5 58 75944.0 59 66417.5 60 52486.5 61 43429.5 62 38029.0 63 29290.0 64 20227.0 65 15147.0 66 11698.0 67 9230.5 68 7178.0 69 5483.5 70 4217.5 71 2987.0 72 2136.0 73 1580.5 74 1157.0 75 875.0 76 686.0 77 476.0 78 323.0 79 228.0 80 141.5 81 86.5 82 48.5 83 25.0 84 19.5 85 15.0 86 12.0 87 9.5 88 8.0 89 4.5 90 1.5 91 2.0 92 1.5 93 1.0 94 1.0 95 2.0 96 2.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006280356411853409 2 0.11015029250841339 3 0.12075856292429014 4 0.1238499300700211 5 0.16416786579171214 6 0.09895628937018765 7 0.028863606929087945 8 0.02814771137955025 9 0.024828559286239123 10-11 0.053919951162907244 12-13 0.05614898957851325 14-15 0.029660854245618557 16-17 0.03403757930983764 18-19 0.039113929570195834 20-21 0.06142058407965444 22-23 0.03384233506905463 24-25 0.05170718310069981 26-27 0.05953322308541871 28-29 0.05624661169890474 30-31 0.0709062001110289 32-33 0.05276475607160777 34-35 0.0661227162118452 36-37 0.05595374533773023 38-39 0.03332168376029995 40-41 0.03958576981875477 42-43 0.034834826626368255 44-45 0.007565714330341541 46-47 0.014578236645131228 48-49 0.009648319565360287 50-51 0.009030046136214097 52-53 0.010673351829471078 54-55 0.01195870974795921 56-57 0.015294132194668922 58-59 0.0203867528084257 60-61 0.025251588474602307 62-63 0.01195870974795921 64-65 0.009550697444968784 66-67 0.010722162889666828 68-69 0.012023791161553546 70-71 0.03166210771364438 72-73 0.029286636117451127 74-75 0.03070215686312793 76-77 0.023803527022128332 78-79 0.005694623689504386 80-81 0.005320405561336955 82-83 0.006117652877867568 84-85 0.005759705103098721 86-87 0.013618285794614774 88-89 0.039341714517776016 90-91 0.037665868117721865 92-93 0.0311414564048897 94-95 0.04946187433169523 96-97 0.045508178455839335 98-99 0.026634568513481942 100-101 0.07764212641804265 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3073074.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.51995424569663 #Duplication Level Percentage of deduplicated Percentage of total 1 76.18112868302275 40.772105215009056 2 13.021615425254895 13.938325235293991 3 4.572639085769855 7.341823039574602 4 2.1512858271432194 4.605468761524829 5 1.1467908397577196 3.0688096636608586 6 0.7038564968356588 2.260222050370847 7 0.4388899571248878 1.644255929695383 8 0.31077258743894215 1.3306027728439154 9 0.2278392338583811 1.0974550834327619 >10 1.124032468387455 10.667739832461013 >50 0.0734485909574648 2.743763360913559 >100 0.03993237400376482 4.148631597998452 >500 0.0038536308511126333 1.371373188958478 >1k 0.0036089558757769844 4.06769781450631 >5k 3.058437182861851E-4 0.9417264537559841 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6744 0.2194545266401004 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 5783 0.18818290740802207 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5670 0.18450580753994208 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5352 0.1741578627784427 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5217 0.16976486736082502 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4845 0.15765972443227858 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4078 0.13270100231885076 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4072 0.13250575807806775 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4010 0.13048823425664333 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3988 0.12977233870710567 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3727 0.12127921423304483 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3663 0.11919660899802609 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3565 0.11600761973190363 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3134 0.10198257510232425 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.762212039150375E-5 2 3.2540706797167916E-5 0.0 0.0 0.0 9.762212039150375E-5 3 3.2540706797167916E-5 0.0 0.0 0.0 9.762212039150375E-5 4 3.2540706797167916E-5 0.0 0.0 0.0 9.762212039150375E-5 5 3.2540706797167916E-5 0.0 0.0 0.0 9.762212039150375E-5 6 3.2540706797167916E-5 0.0 0.0 0.0 9.762212039150375E-5 7 3.2540706797167916E-5 0.0 0.0 3.2540706797167916E-5 9.762212039150375E-5 8 3.2540706797167916E-5 0.0 0.0 3.2540706797167916E-5 9.762212039150375E-5 9 3.2540706797167916E-5 0.0 0.0 3.2540706797167916E-5 9.762212039150375E-5 10-11 3.2540706797167916E-5 0.0 0.0 6.508141359433583E-5 9.762212039150375E-5 12-13 6.508141359433583E-5 0.0 0.0 9.762212039150375E-5 4.881106019575188E-4 14-15 6.508141359433583E-5 0.0 0.0 9.762212039150375E-5 6.670844893419423E-4 16-17 6.508141359433583E-5 0.0 0.0 9.762212039150375E-5 7.8097696313203E-4 18-19 1.1389247379008772E-4 0.0 0.0 9.762212039150375E-5 9.274101437192856E-4 20-21 1.6270353398583959E-4 0.0 0.0 1.3016282718867166E-4 0.0010250322641107894 22-23 1.6270353398583959E-4 0.0 0.0 2.603256543773433E-4 0.0010738433243065414 24-25 2.603256543773433E-4 0.0 0.0 5.694623689504385E-4 0.0010738433243065414 26-27 2.603256543773433E-4 0.0 0.0 0.0011714654446980449 0.0010738433243065414 28-29 3.0913671457309525E-4 0.0 0.0 0.0032052596195210397 0.0010738433243065414 30-31 3.2540706797167917E-4 0.0 0.0 0.011259084551820099 0.0010901136777051253 32-33 3.416774213702631E-4 0.0 0.0 0.031141456404889696 0.0011226543845022931 34-35 3.9048848156601496E-4 0.0 0.0 0.06212020927579355 0.001171465444698045 36-37 4.230291883631829E-4 0.0 0.0 0.10909271953750543 0.001171465444698045 38-39 4.5556989516035086E-4 0.0 0.0 0.182748609372895 0.001236546858292381 40-41 4.5556989516035086E-4 0.0 0.0 0.26296145162791396 0.0012690875650895488 42-43 5.043809553561027E-4 0.0 0.0 0.347860155661725 0.0012853579184881328 44-45 5.206513087546867E-4 0.0 0.0 0.4569366048458319 0.0013504393320824684 46-47 5.206513087546867E-4 0.0 0.0 0.5832108175722419 0.0013992503922782204 48-49 5.531920155518546E-4 0.0 0.0 0.7181083175999017 0.0014317910990753883 50-51 5.531920155518546E-4 0.0 0.0 0.8650621494959119 0.0014643318058725563 52-53 5.531920155518546E-4 0.0 0.0 1.0079158523354792 0.001594494633061228 54-55 5.531920155518546E-4 0.0 0.0 1.1666982311522598 0.0016595760466555638 56-57 5.531920155518546E-4 0.0 0.0 1.356361740719553 0.0017083871068513156 58-59 5.531920155518546E-4 0.0 0.0 1.5542743194599284 0.0017409278136484836 60-61 5.531920155518546E-4 0.0 0.0 1.7648615034978006 0.0017571981670470675 62-63 5.531920155518546E-4 0.0 0.0 1.9748629548133236 0.0018548202874385713 64-65 5.531920155518546E-4 0.0 0.0 2.1968719269370016 0.001903631347634323 66-67 5.531920155518546E-4 0.0 0.0 2.4633315045456112 0.001968712761228659 68-69 5.531920155518546E-4 0.0 0.0 2.751528274294729 0.001984983114627243 70-71 5.531920155518546E-4 0.0 0.0 3.050186881279136 0.002001253468025827 72-73 5.694623689504385E-4 0.0 0.0 3.364123350104814 0.0020988755884173305 74-75 5.857327223490226E-4 0.0 0.0 3.6855604518472385 0.0021964977088088345 76-77 5.857327223490226E-4 0.0 0.0 4.032509467718643 0.00226157912240317 78-79 6.182734291461904E-4 0.0 0.0 4.429066791102329 0.002310390182598922 80-81 6.508141359433583E-4 3.2540706797167916E-5 0.0 4.832766799627995 0.002375471596193258 82-83 6.833548427405263E-4 3.2540706797167916E-5 0.0 5.253989978763935 0.0025056344233819294 84-85 6.996251961391102E-4 6.508141359433583E-5 0.0 5.685772617255556 0.0025381751301790977 86-87 7.158955495376941E-4 9.762212039150375E-5 0.0 6.133727987025369 0.0026846083107663534 88-89 7.158955495376941E-4 1.1389247379008772E-4 0.0 6.604722177207577 0.002798500784556441 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7000 0.0 33.226025 1 GTATCAA 11085 0.0 32.43797 1 TAAGGTG 2130 0.0 28.581419 5 TATCAAC 13215 0.0 26.596878 2 ATCAACG 13215 0.0 26.596878 3 TCAACGC 13260 0.0 26.47075 4 CAACGCA 13375 0.0 26.172031 5 AACGCAG 13965 0.0 25.078691 6 AGGTAAG 2175 0.0 24.709991 2 GTAAGGT 2335 0.0 24.646309 4 GGTAAGG 2415 0.0 23.435986 3 TACATGG 8970 0.0 22.69367 2 GTACATG 9195 0.0 22.65635 1 AAGGTAA 2240 0.0 22.507404 1 ACGCAGA 15545 0.0 22.256006 7 CGCAGAG 15785 0.0 21.857407 8 ACATGGG 8975 0.0 21.621166 3 GAGTACT 8760 0.0 21.182 12-13 TAAGGTA 1150 0.0 20.678812 4 AGAGTAC 12690 0.0 20.409098 10-11 >>END_MODULE