FastQCFastQC Report
Thu 26 May 2016
SRR938475_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938475_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3073074
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66990.2179901948342279No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA58380.1899726462818663No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA57990.18870355871677674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56590.18414785976517326No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA55920.181967632409763No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC51170.16651079668110821No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA49180.16003519602847183No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG45830.14913405925142056No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38590.12557458753027098No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG37520.12209273190297402No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA37340.121506999180625No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA37340.121506999180625No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA36890.12004266737475244No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA34100.11096381017834259No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT31260.1017222494479469No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68700.035.9084431
GTATCAA109350.033.29631
TCAACGC134750.026.8636284
ATCAACG135950.026.626513
TATCAAC137100.026.5564612
CAACGCA136450.026.4941255
AACGCAG141500.025.5696626
TAAGGTG23300.024.4660455
AGGTAAG20500.023.649682
ACGCAGA155900.023.1164997
CGCAGAG159300.022.6529228
GTAAGGT24350.021.6552164
AGAGTAC134600.021.27152810-11
GGTAAGG23300.021.2039073
GAGTACT95850.021.0286812-13
GTACATG93250.020.8478091
AAGGTAA21700.020.8089371
GTGTAGC17400.020.7610991
TACATGG91750.020.3594192
GTACTTT98150.020.34239814-15
Produced by FastQC (version 0.11.5)