##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938475_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3073074 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.748735956244463 31.0 31.0 34.0 30.0 34.0 2 31.971314390737092 31.0 31.0 34.0 30.0 34.0 3 32.038293903758905 33.0 31.0 34.0 30.0 34.0 4 35.724382165870395 37.0 35.0 37.0 33.0 37.0 5 35.61387978291444 37.0 35.0 37.0 33.0 37.0 6 35.61242846739128 37.0 35.0 37.0 33.0 37.0 7 35.54664384912306 37.0 35.0 37.0 33.0 37.0 8 35.51578647308851 37.0 35.0 37.0 33.0 37.0 9 37.09593716259354 39.0 37.0 39.0 33.0 39.0 10-11 36.98900644761565 39.0 37.0 39.0 33.0 39.0 12-13 36.88907344893094 39.0 37.0 39.0 33.0 39.0 14-15 37.930901110744486 40.0 37.0 41.0 33.0 41.0 16-17 37.84280284171484 40.0 37.0 41.0 32.5 41.0 18-19 38.07260124552809 40.0 37.0 41.0 33.0 41.0 20-21 38.0341858022293 40.0 37.0 41.0 33.0 41.0 22-23 37.93655359421869 40.0 37.0 41.0 32.5 41.0 24-25 37.81281430255178 40.0 37.0 41.0 32.0 41.0 26-27 37.61921597071857 40.0 37.0 41.0 32.0 41.0 28-29 37.51080351465666 39.5 36.5 41.0 32.0 41.0 30-31 37.252584871044434 39.0 36.0 41.0 31.0 41.0 32-33 37.04926239979903 39.0 36.0 40.5 31.0 41.0 34-35 36.95432016931581 39.0 36.0 41.0 30.5 41.0 36-37 36.9037906343941 39.0 36.0 41.0 30.0 41.0 38-39 36.73308208653616 39.0 35.0 41.0 30.0 41.0 40-41 36.56460371601855 39.0 35.0 40.5 30.0 41.0 42-43 36.351911799065036 39.0 35.0 40.0 30.0 41.0 44-45 36.070334622596135 38.5 35.0 40.0 29.5 41.0 46-47 35.71577791488262 38.0 35.0 40.0 28.5 41.0 48-49 35.36514171152403 38.0 34.0 40.0 27.0 41.0 50-51 34.98236928235376 38.0 34.0 40.0 26.0 41.0 52-53 34.48940832534459 38.0 33.0 40.0 24.5 41.0 54-55 34.15074905452976 37.5 33.0 40.0 24.0 41.0 56-57 33.81431410372806 37.0 33.0 40.0 23.0 41.0 58-59 33.337452173296185 36.5 32.0 39.0 22.5 41.0 60-61 32.93446090136456 36.0 31.0 39.0 21.5 40.0 62-63 32.45910104995845 35.5 31.0 39.0 20.0 40.0 64-65 31.93762450887938 35.0 30.5 38.5 18.0 40.0 66-67 31.43418528157799 35.0 30.0 38.0 17.0 40.0 68-69 30.94283720470122 34.0 29.0 37.5 13.0 40.0 70-71 30.356788674792732 34.0 29.0 37.0 8.0 39.0 72-73 29.850259544677414 34.0 28.0 36.0 7.5 39.0 74-75 29.263163529417128 34.0 28.0 36.0 7.0 38.5 76-77 28.0579738724157 31.5 26.0 34.5 2.0 36.5 78-79 28.7412232832662 33.0 28.0 35.0 2.0 37.0 80-81 28.367838034489246 33.0 27.0 35.0 2.0 37.0 82-83 27.913360856263143 33.0 26.0 35.0 2.0 36.0 84-85 27.340425580379776 32.5 26.0 35.0 2.0 36.0 86-87 26.73140396228662 32.0 25.0 34.5 2.0 35.0 88-89 25.95693823188117 31.5 23.5 34.0 2.0 35.0 90-91 25.3425177851233 31.0 21.0 34.0 2.0 35.0 92-93 24.51668004089716 31.0 18.5 34.0 2.0 35.0 94-95 23.694732375465087 31.0 9.5 34.0 2.0 35.0 96-97 22.60842270638455 30.0 2.0 34.0 2.0 35.0 98-99 21.222313715842834 29.0 2.0 34.0 2.0 35.0 100-101 17.305739952894072 22.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20876338250344872 1101 2 0.29417436003503283 1101 3 0.24121751782952572 1101 4 0.21132457572771557 1101 5 0.19190121737220522 1101 6 0.2516645674031679 1101 7 0.19848823010886463 1101 8 0.24224160504995496 1101 9 0.2073660506227455 1101 10-11 0.33461168079372783 1101 12-13 0.4447025915248872 1101 14-15 0.4391170303894043 1101 16-17 0.4466145686754217 1101 18-19 0.3966244090219888 1101 20-21 0.3254167717557479 1101 22-23 0.3663044676906466 1101 24-25 0.4745882225437228 1101 26-27 0.4424068895094493 1101 28-29 0.3682077671232378 1101 30-31 0.44920944426555565 1101 32-33 0.3612876194256458 1101 34-35 0.3837214209552897 1101 36-37 0.3915372037297331 1101 38-39 0.46949782823638486 1101 40-41 0.5678597494908928 1101 42-43 0.4835691169261054 1101 44-45 0.4853060993819298 1101 46-47 0.2865088168380652 1101 48-49 0.40831104161761544 1101 50-51 0.4773149292370249 1101 52-53 0.4492422526131037 1101 54-55 0.7189934457995264 1101 56-57 0.5151110552897862 1101 58-59 0.6687597127874838 1101 60-61 0.6745124173067936 1101 62-63 0.7418050274055119 1101 64-65 0.5485370831608236 1101 66-67 0.5911079129303509 1101 68-69 0.8117300669342207 1101 70-71 0.6428693823539966 1101 72-73 0.5222781626474138 1101 74-75 0.5933558293291235 1101 76-77 0.4170200912098956 1101 78-79 0.6712739170851805 1101 80-81 0.8030937799177487 1101 82-83 0.5587727920362475 1101 84-85 0.7227517528379543 1101 86-87 0.8925781529622405 1101 88-89 1.0713510043524046 1101 90-91 1.2364908815869633 1101 92-93 1.5187992358540043 1101 94-95 1.1403524495269721 1101 96-97 1.159485935241399 1101 98-99 1.3014876747267259 1101 100-101 1.3693575019864888 1102 1 0.21259862211231706 1102 2 0.27779208159097024 1102 3 0.1466737954281001 1102 4 0.18613623497096654 1102 5 0.0035955102208191647 1102 6 0.11424549549263929 1102 7 0.17517486348359768 1102 8 0.16444752395833717 1102 9 0.18975883054882559 1102 10-11 0.3040373051849983 1102 12-13 0.3233066891938847 1102 14-15 0.4285946143164523 1102 16-17 0.3815731587101894 1102 18-19 0.32336063817795235 1102 20-21 0.37687981141491633 1102 22-23 0.3649285615747715 1102 24-25 0.3276242953999997 1102 26-27 0.4255930105379875 1102 28-29 0.30131010405827396 1102 30-31 0.27746407470042556 1102 32-33 0.34741539471392713 1102 34-35 0.39752767346886486 1102 36-37 0.41284807788588296 1102 38-39 0.4747876262191113 1102 40-41 0.38479467518455124 1102 42-43 0.2112928834972081 1102 44-45 0.31957412857286727 1102 46-47 0.4026787401667704 1102 48-49 0.5183045197604201 1102 50-51 0.6839224486082145 1102 52-53 0.45271754599499303 1102 54-55 0.36372825431028133 1102 56-57 0.40951223467889264 1102 58-59 0.5159651113349284 1102 60-61 0.5181799595355727 1102 62-63 0.730539930657848 1102 64-65 0.7148520974761254 1102 66-67 0.6143760619837373 1102 68-69 0.7912020070384038 1102 70-71 0.6910844725343459 1102 72-73 0.6892795749189844 1102 74-75 0.8585547950190353 1102 76-77 0.7229545266876514 1102 78-79 0.798220635234486 1102 80-81 0.7202084753654425 1102 82-83 0.88073129426758 1102 84-85 0.9408813196033741 1102 86-87 0.8729338095010526 1102 88-89 0.8586149488893433 1102 90-91 0.5905606783782389 1102 92-93 1.0832251246125537 1102 94-95 1.0648040548823587 1102 96-97 1.0644130595880483 1102 98-99 0.9779896816452514 1102 100-101 1.1528996695254854 1103 1 0.26133907408027923 1103 2 0.23519150107244613 1103 3 0.26994654279113206 1103 4 0.19346737024218896 1103 5 0.15744775789228527 1103 6 0.14768809269433092 1103 7 0.21262547671735632 1103 8 0.13553209455772475 1103 9 0.28060399775724676 1103 10-11 0.2547533876118706 1103 12-13 0.3795505279764839 1103 14-15 0.4996727468859845 1103 16-17 0.4005798437594805 1103 18-19 0.4598297486476852 1103 20-21 0.4483393173486334 1103 22-23 0.4191659952025617 1103 24-25 0.4543024637668083 1103 26-27 0.4489777841864466 1103 28-29 0.3676766032904979 1103 30-31 0.34288233432288706 1103 32-33 0.38357931517276 1103 34-35 0.4383785709804542 1103 36-37 0.43128926648316934 1103 38-39 0.4976119259920395 1103 40-41 0.49599702976840376 1103 42-43 0.4974243855078413 1103 44-45 0.4386129581116478 1103 46-47 0.5047259651704366 1103 48-49 0.6385938566935678 1103 50-51 0.7305029643902969 1103 52-53 0.49493321451590333 1103 54-55 0.3941344757301124 1103 56-57 0.5080580389783123 1103 58-59 0.49119661786587443 1103 60-61 0.40355871431101775 1103 62-63 0.42009276610775004 1103 64-65 0.30998688744796254 1103 66-67 0.33733861958926425 1103 68-69 0.47777268770252945 1103 70-71 0.34315690173998803 1103 72-73 0.33429117478766557 1103 74-75 0.34963983591422476 1103 76-77 0.36802833801501933 1103 78-79 0.6149323224503398 1103 80-81 0.5563124445249947 1103 82-83 0.5369244050277189 1103 84-85 0.6016997667049147 1103 86-87 0.6137467180705265 1103 88-89 0.7106692511598673 1103 90-91 0.8530966868271221 1103 92-93 1.163445487872682 1103 94-95 0.9597376906661168 1103 96-97 1.168142847712943 1103 98-99 1.3529018436315603 1103 100-101 1.4027517318182205 1104 1 0.2377076635937243 1104 2 0.21259451557049758 1104 3 0.26153928310345975 1104 4 0.18144528046001795 1104 5 0.12225446141876972 1104 6 0.08929008060065513 1104 7 0.15249957888477184 1104 8 0.14404858464974524 1104 9 0.2492463856818432 1104 10-11 0.2620270010711465 1104 12-13 0.2809649746766141 1104 14-15 0.36414903931884623 1104 16-17 0.33645180483690496 1104 18-19 0.31715612779034075 1104 20-21 0.3106064139740212 1104 22-23 0.35278883262550664 1104 24-25 0.44842099398685775 1104 26-27 0.2886607331462514 1104 28-29 0.31280170691795206 1104 30-31 0.37303873833612045 1104 32-33 0.4657501715434833 1104 34-35 0.45622805931652266 1104 36-37 0.5836310836680312 1104 38-39 0.5130247277083981 1104 40-41 0.5236303637545063 1104 42-43 0.6516147423344094 1104 44-45 0.5277554124013335 1104 46-47 0.5644062377677415 1104 48-49 0.6903349066545132 1104 50-51 0.8088244281647405 1104 52-53 0.8478540398799908 1104 54-55 0.7835463069930739 1104 56-57 0.6497912339241196 1104 58-59 0.8582021816536027 1104 60-61 0.9336266678075873 1104 62-63 0.7757835178609795 1104 64-65 0.7484985470320566 1104 66-67 0.8029810880223245 1104 68-69 0.9473111130875047 1104 70-71 0.9460274637108377 1104 72-73 0.8806463172114931 1104 74-75 0.972084399308109 1104 76-77 1.0458638135263065 1104 78-79 1.265675176236087 1104 80-81 1.2269842985479151 1104 82-83 1.2878422015327793 1104 84-85 1.3650268551924185 1104 86-87 1.283697912533281 1104 88-89 1.308745642813971 1104 90-91 1.5245744958653944 1104 92-93 1.7851598177267434 1104 94-95 1.770399513769437 1104 96-97 1.7681956133963226 1104 98-99 1.96633098203867 1104 100-101 2.0370356090188757 1105 1 0.2675590329556279 1105 2 0.24116403569632539 1105 3 0.27385931332846525 1105 4 0.23806216701292016 1105 5 0.15274905149733087 1105 6 0.20203217549840957 1105 7 0.22355109811929452 1105 8 0.23347849467398873 1105 9 0.3761156447830061 1105 10-11 0.3956702207441154 1105 12-13 0.3816794788184694 1105 14-15 0.47257347732261934 1105 16-17 0.35987561141355684 1105 18-19 0.38388404114810015 1105 20-21 0.4450079723987699 1105 22-23 0.4346245530316679 1105 24-25 0.4625735718272068 1105 26-27 0.36199269577829085 1105 28-29 0.5043076031948246 1105 30-31 0.5009296047689347 1105 32-33 0.507938896238123 1105 34-35 0.49387207427455593 1105 36-37 0.5237383427838012 1105 38-39 0.5257375995328601 1105 40-41 0.5311998181284139 1105 42-43 0.6786659672508364 1105 44-45 0.6122025275099716 1105 46-47 0.6134366579433532 1105 48-49 0.6387764819034061 1105 50-51 0.7049239306419253 1105 52-53 0.6431578920028898 1105 54-55 0.7311965631646231 1105 56-57 0.7425196461614902 1105 58-59 0.7094688453283808 1105 60-61 0.7191297795430529 1105 62-63 0.6608230785964437 1105 64-65 0.6801019440055924 1105 66-67 0.49560158362477935 1105 68-69 0.6441175530179102 1105 70-71 0.7418385970755494 1105 72-73 0.8230089713762538 1105 74-75 0.8456237935764221 1105 76-77 0.7512210290683861 1105 78-79 1.1976556030390704 1105 80-81 1.0322152808502132 1105 82-83 1.0653474799503933 1105 84-85 1.243867419322978 1105 86-87 1.182463185502879 1105 88-89 1.312055886440472 1105 90-91 1.2740586042537743 1105 92-93 1.5142005599093125 1105 94-95 1.5051281618787549 1105 96-97 1.5988291696251657 1105 98-99 1.5816257123879787 1105 100-101 1.8195017650471286 1106 1 0.009474530249647728 1106 2 0.1254832387670426 1106 3 0.1108724942737922 1106 4 0.04246053925075444 1106 5 -0.03295373698237114 1106 6 -0.005858573868664507 1106 7 0.09095828199814093 1106 8 0.010106331942942859 1106 9 -0.18920694647198388 1106 10-11 0.1337316804406541 1106 12-13 0.09194605942087719 1106 14-15 0.12860471363228498 1106 16-17 0.17132518183297663 1106 18-19 0.17615101063496752 1106 20-21 0.2526909886835398 1106 22-23 0.1570141454312477 1106 24-25 0.15687978135056824 1106 26-27 0.11761978853137833 1106 28-29 0.27083387159368044 1106 30-31 0.2496664813430769 1106 32-33 0.1537232240704114 1106 34-35 0.12428433012240703 1106 36-37 0.1777253977175164 1106 38-39 0.0708946051557291 1106 40-41 0.1659844342572967 1106 42-43 0.2570921205900305 1106 44-45 0.04151112431148363 1106 46-47 -0.5444699047459238 1106 48-49 -0.29399731983989597 1106 50-51 0.25188504312907867 1106 52-53 0.10204786411902234 1106 54-55 0.35731933234927027 1106 56-57 0.37122941891892225 1106 58-59 0.286896164388601 1106 60-61 0.3326666877749247 1106 62-63 0.07783048532244408 1106 64-65 0.14011837577216824 1106 66-67 0.15958884263477202 1106 68-69 -0.13824985204233187 1106 70-71 0.1047363166318469 1106 72-73 0.2958223846370487 1106 74-75 0.375505015999579 1106 76-77 0.21487706294629305 1106 78-79 0.49268588972067207 1106 80-81 -0.03083129008292218 1106 82-83 0.430771912522502 1106 84-85 0.557389192760386 1106 86-87 0.8423285179457345 1106 88-89 0.7802570202965917 1106 90-91 0.8217110898977324 1106 92-93 0.9877820044234014 1106 94-95 1.2737912747175066 1106 96-97 1.3404357037126893 1106 98-99 1.2884099435689045 1106 100-101 1.2390050661623064 1107 1 0.16090364289069825 1107 2 0.12957659026924162 1107 3 0.09622384176535803 1107 4 0.10123505284666123 1107 5 0.09436433132545119 1107 6 0.12499280436716731 1107 7 0.09512462634585717 1107 8 0.14028966696225353 1107 9 0.16666610151486339 1107 10-11 0.19268436203515193 1107 12-13 0.20821562913995706 1107 14-15 0.30704936105502867 1107 16-17 0.2974952492125169 1107 18-19 0.240802737570327 1107 20-21 0.33524421098046986 1107 22-23 0.2851253167085588 1107 24-25 0.28875142303503765 1107 26-27 0.2571505714788245 1107 28-29 0.42783587889692853 1107 30-31 0.2658211567530202 1107 32-33 0.3628510488241048 1107 34-35 0.44130579557278793 1107 36-37 0.3826428939835722 1107 38-39 0.40393418195590414 1107 40-41 0.4272608867256693 1107 42-43 0.27841376713407584 1107 44-45 0.4066607100572526 1107 46-47 0.45572735445206547 1107 48-49 0.6329059983593197 1107 50-51 0.6180993227511848 1107 52-53 0.4670602907542758 1107 54-55 0.3626099747854852 1107 56-57 0.3922369284546079 1107 58-59 0.45215514022756764 1107 60-61 0.5301170456977147 1107 62-63 0.708098019969249 1107 64-65 0.6455874341446233 1107 66-67 0.5641264488264746 1107 68-69 0.7672482646232446 1107 70-71 0.8138913153524889 1107 72-73 0.9919095813349159 1107 74-75 0.8881112572845709 1107 76-77 0.8723510939563504 1107 78-79 1.088699796047841 1107 80-81 0.9354133866956325 1107 82-83 1.1049801484163346 1107 84-85 1.182359142716649 1107 86-87 1.3772821734393403 1107 88-89 1.222963983774381 1107 90-91 1.3345473181511238 1107 92-93 1.520982443173878 1107 94-95 1.369495754851748 1107 96-97 1.4317370094515525 1107 98-99 1.6553699700684845 1107 100-101 1.8484972105056592 1108 1 0.1943811416522756 1108 2 0.15050598096390289 1108 3 0.23429304117091476 1108 4 0.15313079647353334 1108 5 0.09577804164820236 1108 6 0.2215042550397257 1108 7 0.1672162317263357 1108 8 0.17334229051900962 1108 9 0.24208430827476235 1108 10-11 0.26639354162590223 1108 12-13 0.26886227777006866 1108 14-15 0.412160247767666 1108 16-17 0.3938647572718068 1108 18-19 0.3433872362937933 1108 20-21 0.2887003707183098 1108 22-23 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-1.16432594939781 2316 74-75 -1.0736350747561758 2316 76-77 -1.1837612671386815 2316 78-79 -1.3634469006184382 2316 80-81 -1.3663030301350183 2316 82-83 -1.4958717296945565 2316 84-85 -1.6896093649231219 2316 86-87 -1.6607824897161159 2316 88-89 -1.7946194315514639 2316 90-91 -1.725246024539004 2316 92-93 -1.9784174439658386 2316 94-95 -1.789632905872871 2316 96-97 -1.8858019128774188 2316 98-99 -1.9572647749830274 2316 100-101 -2.2799804778842017 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 20.0 4 45.0 5 84.0 6 221.0 7 565.0 8 1363.0 9 2643.0 10 4154.0 11 6414.0 12 9100.0 13 12660.0 14 16204.0 15 21171.0 16 26341.0 17 32084.0 18 30687.0 19 27967.0 20 27962.0 21 29823.0 22 33031.0 23 37556.0 24 42616.0 25 50143.0 26 59447.0 27 71184.0 28 85196.0 29 100873.0 30 119341.0 31 141581.0 32 169204.0 33 206186.0 34 259675.0 35 333755.0 36 427149.0 37 460235.0 38 217367.0 39 9020.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.89736490835525 19.263099800785017 15.06747992718663 23.772055363673104 2 15.722073160130453 20.506429096312562 39.25084333764078 24.520654405916208 3 19.294888211246526 23.577864892893853 30.25762811361787 26.86961878224175 4 13.308726050853315 16.977267713045634 36.47917362224275 33.23483261385831 5 13.563957762371995 37.3599008195798 34.64415963932498 14.431981778723221 6 32.90465276939402 36.43395389566079 16.69475472922381 13.966638605721373 7 28.84665322084662 32.575004702132134 20.298990522193737 18.27935155482751 8 26.5258825527794 35.150080993819216 19.524684403955128 18.799352049446256 9 26.068490378038405 16.69637633197248 19.926301807245775 37.30883148274334 10-11 24.97450435622442 26.448842429437104 27.461411602844578 21.1152416114939 12-13 25.951327563215205 23.651252784670984 27.556707062700088 22.840712589413727 14-15 22.199725746923114 25.386583596750356 25.764836772560635 26.6488538837659 16-17 22.123108652769183 28.473964505898653 26.937066923868414 22.465859917463753 18-19 22.20284965477564 26.870879126242976 29.002295421457475 21.923975797523912 20-21 22.496724777860866 26.120230101845905 29.29597530030191 22.087069819991317 22-23 22.275594404820712 26.1674141267018 29.196172952555 22.360818515922492 24-25 22.29355421645578 26.42336481414648 29.160164887823363 22.122916081574367 26-27 21.69000797586479 26.76374486214054 28.88738801329376 22.658859148700905 28-29 21.973976199382278 27.315488754492044 28.65361251763911 22.056922528486563 30-31 22.04305562809538 26.701397922470193 28.555255579641493 22.700290869792937 32-33 21.642602987281574 26.67535290133308 28.629137425333013 23.052906686052328 34-35 22.601196430073703 26.83535409699112 28.369464948777544 22.193984524157635 36-37 22.85632264249724 26.126993638291523 28.473995534061448 22.542688185149785 38-39 22.037920282699357 26.624511686096714 28.526725082913096 22.810842948290837 40-41 22.631957108326468 26.472676603393896 28.573622766221373 22.32174352205826 42-43 22.293584967549965 26.53983102030828 28.33380843414582 22.832775577995935 44-45 22.28539610469067 26.738807689604087 28.63820056078708 22.33759564491816 46-47 22.88596044221519 27.0521145927498 27.659324181584953 22.40260078345006 48-49 22.369994995239296 27.14906962865196 27.678669631775872 22.80226574433287 50-51 22.626656962966326 27.26907381564499 27.746546636253928 22.357722585134756 52-53 23.144903116553653 26.847498628409213 27.729823622861016 22.27777463217612 54-55 22.30102612113325 27.13724797260293 28.005804696278492 22.55592120998533 56-57 22.507788618171904 27.300709322326767 27.59181848533423 22.599683574167102 58-59 22.40547199718784 26.839573358677505 27.629891582068566 23.125063062066094 60-61 21.836555188713323 27.225247423264133 28.135850291922683 22.802347096099865 62-63 22.118496297173774 27.147170582545883 28.197861355876164 22.536471764404183 64-65 21.723758941534513 27.270415046024553 27.89345436876078 23.112371643680156 66-67 21.660381312737268 27.313213366789412 27.823775999390797 23.202629321082522 68-69 22.51200629052815 26.797038036084615 27.899865779500843 22.791089893886394 70-71 22.187235504809358 26.9849769172271 27.463276963011918 23.364510614951623 72-73 22.10715462990954 26.9911746320377 27.695876407752113 23.205794330300648 74-75 22.190844107275723 27.41242788168919 27.39468920205886 23.002038808976227 76-77 22.20750648900349 26.89575351797997 27.750975283591224 23.14576470942531 78-79 22.31880184913809 27.154430763762257 27.651756075744093 22.875011311355557 80-81 22.671010280903293 27.113243895067768 27.227248307795314 22.98849751623363 82-83 22.410324035629174 27.23396041051089 27.46195690918259 22.893758644677355 84-85 22.277537634070896 27.398622298546027 27.434618089056386 22.88922197832669 86-87 22.604192718677016 27.34542130341601 26.96700169992673 23.083384277980244 88-89 22.287953667337515 27.496046641407943 26.733969702886235 23.482029988368307 90-91 22.125924087508086 27.470036576980505 27.012527310676493 23.391512024834917 92-93 22.388468503890877 27.044532528555003 27.231704030444785 23.33529493710933 94-95 21.98582599097793 26.756640018383965 27.52418998106595 23.73334400957216 96-97 22.302874915117183 26.29485768008016 27.600116150294834 23.802151254507823 98-99 21.984722485399367 26.75056211382146 27.03692597809107 24.227789422688105 100-101 22.01409668174383 26.363079542725178 27.611697180077837 24.01112659545316 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 168.0 1 135.5 2 135.0 3 174.0 4 162.5 5 173.5 6 307.5 7 683.5 8 1119.5 9 1331.0 10 1265.5 11 1100.0 12 1132.5 13 1282.0 14 1417.5 15 1589.5 16 1879.5 17 2261.5 18 2611.5 19 3001.5 20 3597.5 21 4333.0 22 5374.0 23 6842.0 24 8887.0 25 11611.0 26 15322.0 27 20092.5 28 24807.5 29 29901.0 30 35418.5 31 44251.0 32 57832.0 33 62210.0 34 60589.0 35 64763.5 36 71095.5 37 77469.0 38 82419.0 39 86093.0 40 91625.5 41 98863.0 42 102419.5 43 104663.5 44 107257.0 45 113083.5 46 116416.0 47 118513.5 48 121354.5 49 117626.0 50 112614.0 51 108802.0 52 106062.5 53 106616.5 54 108047.5 55 100903.0 56 91081.0 57 82437.5 58 75052.0 59 65434.0 60 50988.0 61 42426.5 62 37390.5 63 27290.0 64 17632.0 65 13077.0 66 10084.0 67 7995.0 68 6126.0 69 4723.5 70 3554.0 71 2277.5 72 1606.0 73 1262.0 74 914.5 75 712.5 76 536.0 77 334.0 78 204.0 79 129.0 80 82.5 81 50.0 82 23.0 83 13.5 84 6.5 85 4.0 86 2.5 87 1.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.9048848156601496E-4 2 0.05359454409493556 3 3.254070679716792E-5 4 0.0 5 0.048811060195751876 6 3.254070679716792E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0064105192390420795 26-27 0.0017246574602498995 28-29 8.135176699291979E-5 30-31 0.0016107649864598118 32-33 4.0675883496459894E-4 34-35 0.011372977025610187 36-37 0.0230225500589963 38-39 0.004148940116638909 40-41 9.762212039150375E-4 42-43 0.0013667096854810524 44-45 0.00813517669929198 46-47 0.0 48-49 0.0 50-51 6.345437825447744E-4 52-53 0.0 54-55 0.0014643318058725563 56-57 0.0 58-59 0.022908657585206216 60-61 0.0 62-63 0.009306642143990023 64-65 0.01545683572865476 66-67 0.006166463938063321 68-69 0.01685608612093298 70-71 9.762212039150374E-5 72-73 3.254070679716792E-5 74-75 0.022518169103640198 76-77 0.04983609245986266 78-79 0.030588264389337844 80-81 0.02682981275426495 82-83 0.035697155356493204 84-85 0.0160262980976052 86-87 0.009583238151765951 88-89 0.08406891601048332 90-91 0.025593265895972564 92-93 0.024665855752253284 94-95 0.02704132734844654 96-97 0.03878852250222416 98-99 0.05310643349297804 100-101 0.014187748163565212 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3073074.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.273441558532404 #Duplication Level Percentage of deduplicated Percentage of total 1 73.52230416278543 36.96219261576438 2 14.236018815379186 14.313873198822666 3 5.141181872466741 7.753947192217288 4 2.400726726643864 4.827711791597484 5 1.2864186210112183 3.2336345681607668 6 0.815135486661708 2.4587799750583947 7 0.5407890420369574 1.9031128410237792 8 0.3778631928998233 1.5197186516295778 9 0.2601195123291399 1.176939279118167 >10 1.2778165535492754 11.358675777375522 >50 0.08465108535624734 2.957074675838423 >100 0.04782619171054428 4.578340414997226 >500 0.004671695576484332 1.5313668782694516 >1k 0.004087733628641123 4.292596266243418 >5k 3.8930796463248796E-4 1.1320358738835943 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6699 0.2179901948342279 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 5838 0.1899726462818663 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5799 0.18870355871677674 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5659 0.18414785976517326 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5592 0.181967632409763 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5117 0.16651079668110821 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4918 0.16003519602847183 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4583 0.14913405925142056 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3859 0.12557458753027098 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3752 0.12209273190297402 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3734 0.121506999180625 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3734 0.121506999180625 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3689 0.12004266737475244 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3410 0.11096381017834259 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3126 0.1017222494479469 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.762212039150375E-5 2 0.0 0.0 0.0 0.0 9.762212039150375E-5 3 0.0 0.0 0.0 0.0 9.762212039150375E-5 4 0.0 0.0 0.0 0.0 9.762212039150375E-5 5 0.0 0.0 0.0 0.0 1.6270353398583959E-4 6 0.0 0.0 0.0 0.0 1.6270353398583959E-4 7 0.0 0.0 0.0 3.2540706797167916E-5 1.6270353398583959E-4 8 0.0 0.0 0.0 3.2540706797167916E-5 1.952442407830075E-4 9 0.0 0.0 0.0 3.2540706797167916E-5 1.952442407830075E-4 10-11 0.0 0.0 0.0 3.2540706797167916E-5 1.952442407830075E-4 12-13 0.0 0.0 0.0 6.508141359433583E-5 4.718402485589348E-4 14-15 0.0 0.0 0.0 6.508141359433583E-5 5.531920155518546E-4 16-17 1.6270353398583958E-5 0.0 0.0 6.508141359433583E-5 6.508141359433583E-4 18-19 3.2540706797167916E-5 0.0 0.0 1.3016282718867166E-4 6.508141359433583E-4 20-21 3.2540706797167916E-5 0.0 0.0 1.6270353398583959E-4 7.97247316530614E-4 22-23 9.762212039150375E-5 0.0 0.0 3.091367145730952E-4 9.111397903207017E-4 24-25 1.952442407830075E-4 0.0 0.0 6.345437825447744E-4 9.111397903207017E-4 26-27 1.952442407830075E-4 0.0 0.0 0.0012690875650895488 9.762212039150375E-4 28-29 3.0913671457309525E-4 0.0 0.0 0.003433044567101215 0.0010738433243065414 30-31 3.2540706797167917E-4 0.0 0.0 0.01142178808580594 0.0010901136777051253 32-33 3.2540706797167917E-4 0.0 0.0 0.03146686347286137 0.0011063840311037092 34-35 3.416774213702631E-4 0.0 0.0 0.06267340129134541 0.001204006151495213 36-37 3.5794777476884707E-4 0.0 0.0 0.11041061816279074 0.001204006151495213 38-39 4.230291883631829E-4 0.0 0.0 0.18514035132248685 0.0012202765048937969 40-41 4.230291883631829E-4 0.0 0.0 0.26660601078919677 0.0012365468582923808 42-43 4.718402485589348E-4 0.0 0.0 0.35267618026770586 0.0012365468582923808 44-45 5.531920155518546E-4 0.0 0.0 0.46243598429455324 0.0012690875650895488 46-47 5.531920155518546E-4 0.0 0.0 0.5894098222171025 0.0013341689786838845 48-49 5.531920155518546E-4 0.0 0.0 0.7245351071923423 0.0013992503922782204 50-51 5.531920155518546E-4 0.0 0.0 0.8725790527660577 0.0014968725126697242 52-53 5.531920155518546E-4 0.0 0.0 1.0156117294930094 0.001529413219466892 54-55 5.531920155518546E-4 0.0 0.0 1.1753215184535093 0.0017083871068513156 56-57 5.531920155518546E-4 0.0 3.2540706797167916E-5 1.3655056793295572 0.0017246574602498997 58-59 5.531920155518546E-4 0.0 3.2540706797167916E-5 1.5643944792738476 0.0017246574602498997 60-61 5.531920155518546E-4 0.0 3.2540706797167916E-5 1.7763971840573967 0.0018548202874385713 62-63 5.857327223490226E-4 0.0 3.2540706797167916E-5 1.9886114034351272 0.002017523821424411 64-65 5.857327223490226E-4 0.0 3.2540706797167916E-5 2.211417622875336 0.0020500645282215788 66-67 6.182734291461904E-4 0.0 3.2540706797167916E-5 2.479650669004391 0.0020500645282215788 68-69 6.182734291461904E-4 0.0 3.2540706797167916E-5 2.770922535545841 0.0020500645282215788 70-71 6.182734291461904E-4 0.0 3.2540706797167916E-5 3.0730629981575452 0.0020826052350187466 72-73 6.182734291461904E-4 0.0 3.2540706797167916E-5 3.390904351798883 0.00226157912240317 74-75 6.182734291461904E-4 0.0 3.2540706797167916E-5 3.7186055395997624 0.00242428265638901 76-77 6.182734291461904E-4 0.0 3.2540706797167916E-5 4.072827403440334 0.0024405530097875937 78-79 6.182734291461904E-4 0.0 3.2540706797167916E-5 4.477747688470893 0.0024405530097875937 80-81 6.833548427405263E-4 0.0 3.2540706797167916E-5 4.892332563420211 0.0024568233631861777 82-83 7.158955495376941E-4 0.0 3.2540706797167916E-5 5.3259049407856764 0.0025381751301790977 84-85 7.158955495376941E-4 0.0 3.2540706797167916E-5 5.771289594718513 0.0025869861903748495 86-87 7.158955495376941E-4 0.0 3.2540706797167916E-5 6.235222451525736 0.0026846083107663534 88-89 7.158955495376941E-4 0.0 3.2540706797167916E-5 6.725073981296903 0.002782230431157857 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6870 0.0 35.908443 1 GTATCAA 10935 0.0 33.2963 1 TCAACGC 13475 0.0 26.863628 4 ATCAACG 13595 0.0 26.62651 3 TATCAAC 13710 0.0 26.556461 2 CAACGCA 13645 0.0 26.494125 5 AACGCAG 14150 0.0 25.569662 6 TAAGGTG 2330 0.0 24.466045 5 AGGTAAG 2050 0.0 23.64968 2 ACGCAGA 15590 0.0 23.116499 7 CGCAGAG 15930 0.0 22.652922 8 GTAAGGT 2435 0.0 21.655216 4 AGAGTAC 13460 0.0 21.271528 10-11 GGTAAGG 2330 0.0 21.203907 3 GAGTACT 9585 0.0 21.02868 12-13 GTACATG 9325 0.0 20.847809 1 AAGGTAA 2170 0.0 20.808937 1 GTGTAGC 1740 0.0 20.761099 1 TACATGG 9175 0.0 20.359419 2 GTACTTT 9815 0.0 20.342398 14-15 >>END_MODULE