FastQCFastQC Report
Thu 26 May 2016
SRR938474_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938474_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3096419
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69290.22377462481660265No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA59230.1912854817129077No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA56260.18169375656201567No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55450.1790778315208633No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA53040.171294647139163No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA47760.15424269131535492No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC43040.1389992762607386No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG42270.13651253270309993No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA40980.13234642985978318No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA39660.12808344090383117No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA37960.12259322785449901No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG37200.12013877966773877No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35700.1152944740359751No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT32130.10376502663237759No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67550.032.77381
GTATCAA105800.032.5800741
ATCAACG128950.026.4658363
TATCAAC130100.026.3049622
TCAACGC129900.026.1990994
CAACGCA130100.026.12065
GTGTAAG14500.025.6109561
AGGTAAG21700.025.40862
AACGCAG135300.025.2510936
TAAGGTG23000.025.2107875
GTACATG94200.024.2093851
TAAGGTA12200.024.1554054
TACATGG93650.023.8038672
GTAAGGT25150.022.6790944
GGTAAGG24750.022.6615333
ACATGGG94800.022.612613
ACGCAGA150200.022.5523347
CGCAGAG152450.022.1883228
GTATAGG8950.021.8102171
AAGGTAA23400.021.7704331
Produced by FastQC (version 0.11.5)