##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938474_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3096419 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.84042146750811 31.0 31.0 34.0 27.0 34.0 2 30.950693365465074 31.0 31.0 34.0 27.0 34.0 3 31.00599046834424 31.0 31.0 34.0 27.0 34.0 4 34.635636843721734 37.0 35.0 37.0 32.0 37.0 5 34.65161271778787 37.0 35.0 37.0 32.0 37.0 6 34.673219612720374 37.0 35.0 37.0 30.0 37.0 7 34.585029351647826 37.0 35.0 37.0 30.0 37.0 8 34.532636894425465 37.0 35.0 37.0 30.0 37.0 9 36.01557121306903 39.0 35.0 39.0 30.0 39.0 10-11 35.85744855589634 38.0 35.0 39.0 30.0 39.0 12-13 35.70914853577632 38.0 35.0 39.0 30.0 39.0 14-15 36.66039512094455 39.0 36.0 41.0 30.0 41.0 16-17 36.50830313985284 39.0 36.0 40.5 30.0 41.0 18-19 36.49792518389792 39.0 36.0 40.5 29.5 41.0 20-21 36.568684180015694 39.0 36.0 41.0 30.0 41.0 22-23 36.48601174453457 39.0 36.0 41.0 29.5 41.0 24-25 36.363880501960494 39.0 35.5 41.0 29.0 41.0 26-27 36.16862430439808 39.0 35.0 41.0 28.0 41.0 28-29 35.98397955832205 39.0 35.0 40.0 27.0 41.0 30-31 35.748675324625 38.5 35.0 40.0 27.0 41.0 32-33 35.45916282647794 38.0 34.0 40.0 26.5 41.0 34-35 35.19628577398601 38.0 34.0 40.0 25.5 41.0 36-37 34.88495064782899 38.0 34.0 40.0 25.0 41.0 38-39 34.61279465085313 38.0 33.0 40.0 24.0 41.0 40-41 34.53431447746574 38.0 33.0 40.0 24.0 41.0 42-43 34.344805402628 38.0 33.0 40.0 24.0 41.0 44-45 34.064644997979926 38.0 33.0 40.0 23.0 41.0 46-47 33.706902069778025 38.0 33.0 40.0 21.0 41.0 48-49 33.33479561390109 38.0 32.0 40.0 18.5 41.0 50-51 32.597504246033886 36.5 30.5 39.5 16.5 40.5 52-53 32.66635151767251 37.0 31.0 39.0 17.0 40.0 54-55 32.79127437210532 37.0 31.5 40.0 14.5 41.0 56-57 32.48737331737081 37.0 31.0 40.0 12.5 41.0 58-59 32.1538658043372 36.0 31.0 39.0 8.5 41.0 60-61 31.69708088601704 36.0 30.5 39.0 7.5 41.0 62-63 31.17770560121224 35.0 29.5 39.0 6.0 40.0 64-65 30.68379424748395 35.0 29.0 38.5 2.0 40.0 66-67 30.143772047646006 35.0 28.0 38.0 2.0 40.0 68-69 29.58269536519444 34.0 28.0 37.5 2.0 40.0 70-71 28.938124330072903 34.0 26.5 37.0 2.0 39.0 72-73 28.38912902291324 34.0 26.0 36.0 2.0 39.0 74-75 27.798319445785598 33.0 25.5 36.0 2.0 39.0 76-77 27.074968213281213 32.5 24.5 35.0 2.0 38.0 78-79 26.297400965437816 32.0 22.0 35.0 2.0 37.0 80-81 25.64550453281678 31.5 20.0 35.0 2.0 36.5 82-83 24.983674205590393 31.0 18.0 34.5 2.0 36.0 84-85 24.33330873502585 31.0 13.5 34.0 2.0 35.5 86-87 23.68436829770131 30.5 7.0 34.0 2.0 35.0 88-89 22.884064139898378 30.0 2.0 34.0 2.0 35.0 90-91 22.055416434274562 29.0 2.0 34.0 2.0 35.0 92-93 21.22057011664119 29.0 2.0 34.0 2.0 35.0 94-95 20.27240838529928 28.0 2.0 34.0 2.0 35.0 96-97 19.052615779711985 25.5 2.0 33.0 2.0 35.0 98-99 17.602647929753694 22.5 2.0 32.5 2.0 34.0 100-101 14.32027852173753 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6829744063301177 1101 2 0.6639016650572991 1101 3 0.7750009336635593 1101 4 0.6724523835341429 1101 5 0.6012051159435288 1101 6 0.6741360098765057 1101 7 0.6308671267604211 1101 8 0.7059271644918539 1101 9 0.7682055546795112 1101 10-11 0.832499863434137 1101 12-13 0.747944051041749 1101 14-15 1.0427122885800841 1101 16-17 0.9717343367475522 1101 18-19 1.0429713300805403 1101 20-21 1.053479288026466 1101 22-23 1.1814790057912958 1101 24-25 1.1336731486296685 1101 26-27 1.1915118179663935 1101 28-29 1.1332560414183206 1101 30-31 1.1226437411030403 1101 32-33 1.3048470636686744 1101 34-35 1.2298628908398328 1101 36-37 1.1411007149123265 1101 38-39 1.2260328992661726 1101 40-41 1.3099447980033858 1101 42-43 1.3002799370984164 1101 44-45 1.3459792700648734 1101 46-47 1.2964078790180977 1101 48-49 1.2923632604631763 1101 50-51 1.274299367192718 1101 52-53 1.3591013860152756 1101 54-55 1.272277590174511 1101 56-57 1.3118522186316355 1101 58-59 1.3797029453453433 1101 60-61 1.425375428225582 1101 62-63 1.2689652064415498 1101 64-65 1.2787975799888507 1101 66-67 1.0281948932571652 1101 68-69 1.0950485936005414 1101 70-71 1.4138804903112607 1101 72-73 1.497200134671477 1101 74-75 1.3544824688593522 1101 76-77 1.55402584375339 1101 78-79 1.408792868267227 1101 80-81 1.6676859911227098 1101 82-83 1.5452895591244165 1101 84-85 1.5936604514210408 1101 86-87 1.71992909760948 1101 88-89 1.761505336202351 1101 90-91 1.7084889200568405 1101 92-93 1.8625067054965925 1101 94-95 1.7427840745714498 1101 96-97 1.6293232005534044 1101 98-99 1.5920470132338878 1101 100-101 1.2916057728401444 1102 1 0.5829717537901224 1102 2 0.6675522232274993 1102 3 0.6249439040536338 1102 4 0.7241712294518905 1102 5 0.6189951332987178 1102 6 0.7273137527923268 1102 7 0.5193709236819615 1102 8 0.59427465102074 1102 9 0.7615145225382989 1102 10-11 0.7233890516811101 1102 12-13 0.8112031037818213 1102 14-15 0.867419419744401 1102 16-17 0.8862773135795123 1102 18-19 0.9362281471083307 1102 20-21 0.8838668809600989 1102 22-23 1.0249047801435083 1102 24-25 0.86122863408238 1102 26-27 0.8882587618613869 1102 28-29 0.7721674295303913 1102 30-31 0.7620340074180518 1102 32-33 0.8253521451774333 1102 34-35 0.7811881187688101 1102 36-37 0.8336362778949891 1102 38-39 0.9108919641321407 1102 40-41 0.9861217678932732 1102 42-43 0.9794140154165589 1102 44-45 1.1370780089715709 1102 46-47 1.199892842905662 1102 48-49 0.9710338547837054 1102 50-51 1.0370164018045713 1102 52-53 0.9894450699386113 1102 54-55 0.9739502061094498 1102 56-57 0.985676243444253 1102 58-59 1.114320108036992 1102 60-61 1.1597185740622287 1102 62-63 1.1161369830370518 1102 64-65 0.8644630170137617 1102 66-67 0.9149345142470899 1102 68-69 0.8766500551500798 1102 70-71 0.8518683682034727 1102 72-73 1.0378649938216036 1102 74-75 0.9581326007284829 1102 76-77 0.8251137735596004 1102 78-79 0.8374317268413698 1102 80-81 1.021651348950357 1102 82-83 1.1398481101554978 1102 84-85 1.1135582907377461 1102 86-87 0.9566015051305676 1102 88-89 1.2386925448966188 1102 90-91 1.0971881049691916 1102 92-93 1.2064274627588674 1102 94-95 1.158353963316344 1102 96-97 1.5810003163329824 1102 98-99 1.491921917552986 1102 100-101 1.004722204188619 1103 1 0.6355308098113355 1103 2 0.7612032134968487 1103 3 0.6481910332588932 1103 4 0.6526922253641843 1103 5 0.6730832918152174 1103 6 0.717751930348058 1103 7 0.6581655339741559 1103 8 0.6325090950522849 1103 9 0.750512600264237 1103 10-11 0.7737254496422992 1103 12-13 0.7760470509124033 1103 14-15 1.0020688685242902 1103 16-17 0.9501438371078734 1103 18-19 0.8802333129331501 1103 20-21 0.8626191002920365 1103 22-23 1.065331488283583 1103 24-25 1.1083365062305077 1103 26-27 1.1135038632739622 1103 28-29 1.069228735850956 1103 30-31 1.0669820280262954 1103 32-33 1.2260932988171476 1103 34-35 1.1990343001415624 1103 36-37 1.2065985068153537 1103 38-39 1.1211266971929632 1103 40-41 1.2096740982491454 1103 42-43 1.0110051445117065 1103 44-45 1.0727907872769435 1103 46-47 1.0127263161658746 1103 48-49 0.9300777184169462 1103 50-51 0.9833761239694425 1103 52-53 1.1796820739437308 1103 54-55 1.1486366322854096 1103 56-57 1.067602394170727 1103 58-59 1.0746779079278 1103 60-61 1.2441111079314133 1103 62-63 1.5151219397514666 1103 64-65 1.2105407062476523 1103 66-67 1.3129085566655796 1103 68-69 1.2781269718041308 1103 70-71 1.33196124753146 1103 72-73 1.3151834215023506 1103 74-75 1.2453506251445745 1103 76-77 1.28006741418152 1103 78-79 1.331679585501469 1103 80-81 1.365634493497108 1103 82-83 1.4835985402585834 1103 84-85 1.4688456913552592 1103 86-87 1.4393915322442652 1103 88-89 1.4865709323885348 1103 90-91 1.618539110378169 1103 92-93 1.7738788668447327 1103 94-95 1.7666531177910194 1103 96-97 1.7580105608150483 1103 98-99 1.6744936816867444 1103 100-101 1.2214229350197847 1104 1 0.7719930890629847 1104 2 0.8040752939042619 1104 3 0.7650623641353107 1104 4 0.804419151336532 1104 5 0.6733137234292599 1104 6 0.7593359319039337 1104 7 0.7347501200768036 1104 8 0.710574496548503 1104 9 0.7309738786844093 1104 10-11 0.8038315202970665 1104 12-13 0.8263328232625753 1104 14-15 1.0071779711739808 1104 16-17 0.9877537400949592 1104 18-19 1.0363408979464594 1104 20-21 1.10138334657087 1104 22-23 1.10684452476913 1104 24-25 1.1279323167237223 1104 26-27 1.0807281620636715 1104 28-29 1.1198343700077231 1104 30-31 1.2142189246183364 1104 32-33 1.1630766441509834 1104 34-35 1.1629667811656432 1104 36-37 1.2480357161125966 1104 38-39 1.1515979183712588 1104 40-41 1.017283695305217 1104 42-43 1.047806406258644 1104 44-45 1.1221022239617895 1104 46-47 1.0685453426715128 1104 48-49 1.0456803096526741 1104 50-51 0.8448020465973016 1104 52-53 0.9249468627038269 1104 54-55 0.997453389438455 1104 56-57 1.1796844842040386 1104 58-59 0.9982704340330031 1104 60-61 1.1377547354429218 1104 62-63 1.2263439812073074 1104 64-65 1.0613779192597725 1104 66-67 1.3414650867085491 1104 68-69 1.4071153562589025 1104 70-71 1.2992282127722312 1104 72-73 1.227239179615804 1104 74-75 1.0284897287011319 1104 76-77 1.2886509098586032 1104 78-79 1.3243890854244391 1104 80-81 1.3540939013676194 1104 82-83 1.338019079593117 1104 84-85 1.3700186090303141 1104 86-87 1.4404462840631886 1104 88-89 1.5176120593509985 1104 90-91 1.580446218794659 1104 92-93 1.575329853963943 1104 94-95 1.4824491557658028 1104 96-97 1.2717999644571556 1104 98-99 1.3006930678580062 1104 100-101 1.0888344526670881 1105 1 0.553283428174467 1105 2 0.6312257850456966 1105 3 0.5842887995851882 1105 4 0.5920291252983958 1105 5 0.5129213351348199 1105 6 0.6364325841825789 1105 7 0.5474278652957238 1105 8 0.563012205779799 1105 9 0.6574222019514977 1105 10-11 0.6040770961807311 1105 12-13 0.7274864895641144 1105 14-15 0.8663213102497096 1105 16-17 0.8866892266521873 1105 18-19 0.8561751515307066 1105 20-21 0.9317385983447863 1105 22-23 0.837713389582234 1105 24-25 0.918520992151322 1105 26-27 0.9726937837129483 1105 28-29 1.0357299765457455 1105 30-31 0.9976421030391478 1105 32-33 0.8828813695314537 1105 34-35 0.9574517743704334 1105 36-37 1.0748351336486266 1105 38-39 1.1719696927146188 1105 40-41 0.9816671850512364 1105 42-43 1.07251938447245 1105 44-45 1.0211924804604138 1105 46-47 1.0408185866267559 1105 48-49 0.8819571647687994 1105 50-51 0.8354480736901024 1105 52-53 0.932454651309989 1105 54-55 0.9456105216361692 1105 56-57 1.0404994006375645 1105 58-59 1.1507688837506436 1105 60-61 1.217495401771938 1105 62-63 1.1422553953561732 1105 64-65 1.2071642817756079 1105 66-67 1.0719631958909943 1105 68-69 0.8894170828426091 1105 70-71 1.0162037890836295 1105 72-73 0.9705230630229984 1105 74-75 0.8724117333293506 1105 76-77 0.942071056937575 1105 78-79 1.0371815856326272 1105 80-81 1.2602121389905179 1105 82-83 1.3823058060972713 1105 84-85 1.035657264403941 1105 86-87 1.3737142482764106 1105 88-89 1.4312257907716308 1105 90-91 1.3920729645283068 1105 92-93 1.637715416676663 1105 94-95 1.5819883380804534 1105 96-97 1.606032006959584 1105 98-99 1.5370560072966732 1105 100-101 1.2404994900133435 1106 1 0.22191673948105617 1106 2 0.3672393082535983 1106 3 0.2647371347473495 1106 4 0.39628695540147874 1106 5 0.45872766549960176 1106 6 0.13927201326555405 1106 7 0.2531552227129765 1106 8 0.36548910295936565 1106 9 0.4495220780763276 1106 10-11 0.17438312645639087 1106 12-13 0.255219371854416 1106 14-15 0.5014656864584026 1106 16-17 0.2631386975051484 1106 18-19 -0.004929156213215435 1106 20-21 0.32159417161604154 1106 22-23 0.36925083605623854 1106 24-25 0.4760392854626687 1106 26-27 0.26747182891021026 1106 28-29 0.27223930355800974 1106 30-31 0.30532923436906145 1106 32-33 0.4531141457296428 1106 34-35 0.29566898230896044 1106 36-37 0.10959090960633944 1106 38-39 0.3871107548078001 1106 40-41 0.3376811932516546 1106 42-43 -0.06344109267934783 1106 44-45 0.27283993940277185 1106 46-47 -0.11645882706984167 1106 48-49 0.3831908644735549 1106 50-51 0.08635036184436018 1106 52-53 0.4469964445293897 1106 54-55 0.21791815642872336 1106 56-57 -0.33677748765046545 1106 58-59 -0.7498559482701772 1106 60-61 -0.003719787385243478 1106 62-63 0.13988499444923974 1106 64-65 0.4337544004573175 1106 66-67 -0.08333130570846947 1106 68-69 -0.2936244466621112 1106 70-71 -0.24386071492059003 1106 72-73 0.1383570460442236 1106 74-75 0.1867196187013711 1106 76-77 0.31802300630435454 1106 78-79 0.46671864007101505 1106 80-81 0.40586711127521724 1106 82-83 0.45476974993513863 1106 84-85 0.424957117087466 1106 86-87 0.6397852835131381 1106 88-89 0.4574450922611639 1106 90-91 0.7067885467315058 1106 92-93 0.6318719641766251 1106 94-95 0.2881454190721833 1106 96-97 0.6487827834528055 1106 98-99 0.5595428872387984 1106 100-101 -0.014852402366818396 1107 1 0.49072377881285334 1107 2 0.38888756497295063 1107 3 0.334515568958313 1107 4 0.36091419559399185 1107 5 0.3793244102787696 1107 6 0.37650058978714185 1107 7 0.3554380692388506 1107 8 0.370307683849191 1107 9 0.4003783903800766 1107 10-11 0.41002640377307387 1107 12-13 0.38854189885229573 1107 14-15 0.4176300380880704 1107 16-17 0.4368244544938449 1107 18-19 0.5656674906233405 1107 20-21 0.5078346050708831 1107 22-23 0.6081071209360545 1107 24-25 0.5186970171508278 1107 26-27 0.4732922648332121 1107 28-29 0.4770483840139548 1107 30-31 0.5924643736336677 1107 32-33 0.5878662431707937 1107 34-35 0.7968334220869835 1107 36-37 0.7944228302806522 1107 38-39 0.7955599705812659 1107 40-41 0.6268198466973729 1107 42-43 0.6494816521429172 1107 44-45 0.7523998048687943 1107 46-47 0.7889392802870248 1107 48-49 0.758124508350285 1107 50-51 0.6169623269062754 1107 52-53 0.6539677062065934 1107 54-55 0.7304215109633674 1107 56-57 0.6295519952320987 1107 58-59 0.7055241376450851 1107 60-61 0.760877090595887 1107 62-63 0.8456384376219575 1107 64-65 0.6879016826476274 1107 66-67 0.73130925468001 1107 68-69 0.8446017733601039 1107 70-71 0.7880097148320253 1107 72-73 0.7265197992503722 1107 74-75 0.8011137554645238 1107 76-77 0.7575699825219324 1107 78-79 0.6942066274089917 1107 80-81 0.7206849478011534 1107 82-83 0.8537840554338345 1107 84-85 0.6867641975551599 1107 86-87 0.677149314674562 1107 88-89 0.8410717310860143 1107 90-91 0.8334818276720704 1107 92-93 0.9206227648575229 1107 94-95 0.8315556438896685 1107 96-97 0.9965229534420139 1107 98-99 0.8999239427859749 1107 100-101 0.4734388515533361 1108 1 0.42201771781209985 1108 2 0.3841597515455497 1108 3 0.4166799208339569 1108 4 0.46577270596650067 1108 5 0.5577296646173764 1108 6 0.43930706842387224 1108 7 0.42654942867955725 1108 8 0.3995752782760533 1108 9 0.3081056744044943 1108 10-11 0.43076496671587705 1108 12-13 0.4847759338392379 1108 14-15 0.4825280135561414 1108 16-17 0.47978551581134354 1108 18-19 0.5113681329519224 1108 20-21 0.4834234374149631 1108 22-23 0.5726003077457946 1108 24-25 0.4468389715242509 1108 26-27 0.5052107517236237 1108 28-29 0.29544377348020134 1108 30-31 0.48439132387071737 1108 32-33 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90-91 0.2022565594811141 2312 92-93 0.21499904286595495 2312 94-95 0.09104608556291538 2312 96-97 0.042267888011931376 2312 98-99 0.0013505351310527658 2312 100-101 0.3573470170506905 2313 1 -0.6509313955849514 2313 2 -0.5110317044603718 2313 3 -0.6915837781849206 2313 4 -0.5639193537999887 2313 5 -0.5626349871008145 2313 6 -0.5901443809143103 2313 7 -0.6436077278271597 2313 8 -0.5455899447578361 2313 9 -0.7750888669922062 2313 10-11 -0.7372201265206684 2313 12-13 -0.6320385521241079 2313 14-15 -0.8642548498330953 2313 16-17 -0.8901723684257234 2313 18-19 -0.872017591842301 2313 20-21 -0.8569480786720618 2313 22-23 -0.9084800438028395 2313 24-25 -0.9424760752973569 2313 26-27 -0.9862734982144801 2313 28-29 -0.8446219296126145 2313 30-31 -0.8645193597864491 2313 32-33 -0.8197349262537159 2313 34-35 -0.8300273815147889 2313 36-37 -0.9257772520681229 2313 38-39 -0.8949758538324488 2313 40-41 -0.8963415551017135 2313 42-43 -0.9281591274015071 2313 44-45 -0.8203289961654221 2313 46-47 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6 -0.5630790469321312 2314 7 -0.7134200292557225 2314 8 -0.5765858996544679 2314 9 -0.7380159150009504 2314 10-11 -0.7803381003510594 2314 12-13 -0.897485827772897 2314 14-15 -1.0281504550826384 2314 16-17 -1.034185479554388 2314 18-19 -1.0573046430103545 2314 20-21 -0.9384481215605192 2314 22-23 -1.0051519844757948 2314 24-25 -1.0815187115191591 2314 26-27 -0.9196705272758692 2314 28-29 -1.0068533778071753 2314 30-31 -0.9089035622267616 2314 32-33 -0.9650689593907913 2314 34-35 -0.9519058449700069 2314 36-37 -0.9112512714299257 2314 38-39 -0.7987526283317337 2314 40-41 -0.7226319668563761 2314 42-43 -0.9094403117683711 2314 44-45 -1.0253345886503453 2314 46-47 -0.8421003722743094 2314 48-49 -0.7732402242958543 2314 50-51 -0.79508000985631 2314 52-53 -0.9034634047103793 2314 54-55 -0.8493907725111569 2314 56-57 -0.8712339819338126 2314 58-59 -0.9227440420609163 2314 60-61 -0.9027312965697369 2314 62-63 -0.9636576489344364 2314 64-65 -0.9403259241943935 2314 66-67 -0.9141504110102439 2314 68-69 -0.8631692884397388 2314 70-71 -0.7432485695342095 2314 72-73 -0.8752607368902829 2314 74-75 -0.6532457676969017 2314 76-77 -0.5878106948158432 2314 78-79 -0.7443510969451843 2314 80-81 -0.7068900041284358 2314 82-83 -0.6929940596521149 2314 84-85 -0.8122284945351552 2314 86-87 -0.8585292811243619 2314 88-89 -0.886218518304819 2314 90-91 -0.8352646199828087 2314 92-93 -0.6637715888203033 2314 94-95 -0.49725936391505954 2314 96-97 -0.6550108339877845 2314 98-99 -0.6346671759650278 2314 100-101 -0.2590781948202938 2315 1 -0.4776867512335947 2315 2 -0.44337188733596733 2315 3 -0.5893275567889766 2315 4 -0.5233370489652103 2315 5 -0.4602626404799963 2315 6 -0.49225990214956994 2315 7 -0.6310479454018747 2315 8 -0.5224191550111215 2315 9 -0.6025077459078645 2315 10-11 -0.8259228814228834 2315 12-13 -0.819997006637557 2315 14-15 -0.8296383269961396 2315 16-17 -1.1031917627227799 2315 18-19 -0.8212396307139684 2315 20-21 -0.6993498806032719 2315 22-23 -0.823688121688086 2315 24-25 -0.6353511178059392 2315 26-27 -0.7921405045459906 2315 28-29 -0.750930376208423 2315 30-31 -0.9746744328233277 2315 32-33 -0.874072099564998 2315 34-35 -0.8257503908368236 2315 36-37 -0.8515104547966317 2315 38-39 -0.8780808017880943 2315 40-41 -0.8959655326243023 2315 42-43 -0.9377548610542021 2315 44-45 -0.8507003719032156 2315 46-47 -0.9762294437143666 2315 48-49 -1.1836001159300622 2315 50-51 -1.0910981489832778 2315 52-53 -1.070022014929279 2315 54-55 -1.0728296302934055 2315 56-57 -0.9476884691476428 2315 58-59 -0.808585374519545 2315 60-61 -0.7675886565041843 2315 62-63 -0.9667904741076505 2315 64-65 -0.8599143662819095 2315 66-67 -0.8367691335993079 2315 68-69 -0.8242213694257607 2315 70-71 -0.940684021295187 2315 72-73 -0.7064762663920874 2315 74-75 -0.5533858437183916 2315 76-77 -0.4772923203915802 2315 78-79 -0.6807972848769239 2315 80-81 -0.5568736012536668 2315 82-83 -0.4862058929312276 2315 84-85 -0.7147801648724936 2315 86-87 -0.666513614349423 2315 88-89 -0.6804597719065946 2315 90-91 -0.7390504154436179 2315 92-93 -0.8772195619693228 2315 94-95 -1.1204604633306694 2315 96-97 -1.1362993795685057 2315 98-99 -1.1568632265082712 2315 100-101 -0.836380000110692 2316 1 -0.9048662196983805 2316 2 -1.02944417174961 2316 3 -0.7402356647456436 2316 4 -0.7249350434372701 2316 5 -0.6244690775886923 2316 6 -0.934720225098026 2316 7 -0.6910942200896386 2316 8 -0.7640132306518836 2316 9 -0.929028213637153 2316 10-11 -1.0395471463408796 2316 12-13 -0.9004388263136676 2316 14-15 -1.298946257998658 2316 16-17 -1.2844448482893753 2316 18-19 -1.2197084847939763 2316 20-21 -1.2888914282007846 2316 22-23 -1.1541341127067142 2316 24-25 -0.8915568485970411 2316 26-27 -1.2470202302420716 2316 28-29 -1.5772147057996193 2316 30-31 -1.2426134585922384 2316 32-33 -1.247166119483758 2316 34-35 -1.3542911178175032 2316 36-37 -1.338680788244389 2316 38-39 -1.4942097281255826 2316 40-41 -1.3347729194785316 2316 42-43 -1.332273832989138 2316 44-45 -1.293605667795049 2316 46-47 -1.2957368808834602 2316 48-49 -1.2901296455105893 2316 50-51 -1.2206876183911888 2316 52-53 -1.2711030752229817 2316 54-55 -1.3448181150681826 2316 56-57 -1.380150775602928 2316 58-59 -1.2683460616998161 2316 60-61 -1.2976569871097148 2316 62-63 -1.2146975978059373 2316 64-65 -1.2281048950055968 2316 66-67 -1.1851154483587258 2316 68-69 -1.1771377802723926 2316 70-71 -1.4455319689455983 2316 72-73 -1.3216030444212556 2316 74-75 -1.2398372250930407 2316 76-77 -1.0986219070414265 2316 78-79 -0.9122853798653985 2316 80-81 -1.0185857629165191 2316 82-83 -1.0053733679163344 2316 84-85 -1.1647295867868799 2316 86-87 -1.0812093923172945 2316 88-89 -1.2304448130708288 2316 90-91 -1.0601511716120946 2316 92-93 -1.0982466437525318 2316 94-95 -1.2174890130695353 2316 96-97 -1.3356347218679794 2316 98-99 -1.3634265908070944 2316 100-101 -1.2677315695125078 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12015.0 3 4507.0 4 4680.0 5 5119.0 6 6081.0 7 7511.0 8 9716.0 9 12186.0 10 15042.0 11 18163.0 12 22149.0 13 25283.0 14 28784.0 15 32434.0 16 36413.0 17 40558.0 18 39443.0 19 38204.0 20 40217.0 21 43358.0 22 47735.0 23 53078.0 24 59045.0 25 66480.0 26 73591.0 27 83699.0 28 95131.0 29 108572.0 30 124917.0 31 145102.0 32 170383.0 33 203248.0 34 247262.0 35 300835.0 36 351560.0 37 345745.0 38 168133.0 39 10040.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.222477101323605 18.42082444941261 14.437570976999409 23.919127472264375 2 15.354929346284308 19.671245517703976 40.22360267713188 24.750222458879843 3 19.27889679416345 23.259827204053014 29.768948426161074 27.69232757562246 4 12.861302354166376 16.23113657021382 36.78806234047895 34.11949873514085 5 13.642296236909907 37.199376593697885 34.580824536589574 14.577502632802636 6 32.95234869788957 35.854297290816625 17.038538177206977 14.154815834086836 7 28.91305942097766 32.471914867634084 20.320121887196837 18.294903824191415 8 26.57932101706907 34.69762047448363 19.723360572985737 18.99969793546157 9 26.268269405357064 16.690510021639945 19.995367022895437 37.04585355010755 10-11 24.983744732283615 26.21187564223452 27.540680170479593 21.263699455002268 12-13 26.113091825623247 23.538222190130178 27.48555979159771 22.863126192648863 14-15 22.382296928178793 25.22421209570992 25.779617787067565 26.613873189043723 16-17 22.24320468611591 28.27135833913218 26.949495419394065 22.535941555357848 18-19 22.37616603445385 26.76315006430898 28.916555985000063 21.944127916237104 20-21 22.62792696862202 26.042263926834853 29.194521305719174 22.135287798823956 22-23 22.313039685296328 26.090033037423566 29.132820135865394 22.464107141414715 24-25 22.2932841767021 26.266071146661478 29.19415582033764 22.246488856298775 26-27 21.728428770148682 26.609269404695933 28.87926702974824 22.783034795407143 28-29 21.944616537818764 27.28246567309331 28.61853131969277 22.154386469395156 30-31 22.06164114105502 26.57254543354226 28.539219828306972 22.826593597095744 32-33 21.506793168978554 26.671320543841254 28.64449553549287 23.177390751687327 34-35 22.66365958354268 26.831286158101275 28.274023680779603 22.231030577576444 36-37 22.896575121163167 26.063747980613893 28.381017770597737 22.658659127625203 38-39 22.101810584013403 26.612635679123255 28.36192362720234 22.923630109661 40-41 22.730325373303074 26.4215720319478 28.507046120797565 22.34105647395156 42-43 22.319262893596157 26.485868993963074 28.250318740246804 22.94454937219396 44-45 22.28188509041395 26.750915976283203 28.58585599233146 22.381342940971386 46-47 22.901386164325224 27.057117566947557 27.603618338976794 22.437877929750428 48-49 22.34446822182699 27.127727178840207 27.671075629679404 22.856728969653396 50-51 22.581413548560676 27.300616865468164 27.70361905820059 22.414350527770573 52-53 23.048405902891048 26.816618272606597 27.764549709151158 22.3704261153512 54-55 22.225071720139972 27.156642857927267 28.054896833461346 22.56338858847142 56-57 22.447055540509968 27.296197804657435 27.60970051310573 22.647046141726868 58-59 22.429017109793957 26.758079535203255 27.6962225897858 23.116680765216987 60-61 21.78390589122945 27.140814004302865 28.24333826322795 22.831941841239736 62-63 22.142438670229637 27.057120544754472 28.31142649890482 22.489014286111072 64-65 21.76535763245681 27.26000048123685 27.970131663495295 23.00451022281105 66-67 21.723440602745114 27.21438051636138 27.94140943361279 23.120769447280715 68-69 22.623385788560405 26.687472193131267 28.041403266817554 22.64773875149077 70-71 22.205773022907547 26.87761661115735 27.57792072393992 23.338689641995185 72-73 22.17238067827784 26.88129217190584 27.859307302674484 23.087019847141832 74-75 22.201161413173825 27.292450491351584 27.694414767115745 22.811973328358846 76-77 22.251245062627 26.77817294347049 27.853146724400833 23.117435269501673 78-79 22.39089603858309 27.133444229903738 27.67299517792085 22.80266455359232 80-81 22.712865134627016 27.05087912006281 27.315023701473507 22.921232043836664 82-83 22.39692790161017 27.14986330260266 27.651969879741817 22.801238916045353 84-85 22.318381399464837 27.280521145706672 27.628570644213735 22.77252681061476 86-87 22.6751377996371 27.222687314165086 27.194684903438514 22.907489982759298 88-89 22.386668915068554 27.280771124317244 26.99340533388122 23.33915462673298 90-91 22.294338169445265 27.279405968486657 27.183971694077908 23.242284167990167 92-93 22.576245245877068 26.76274834982592 27.52641215298214 23.134594251314873 94-95 22.188791655420395 26.523463880531246 27.77109327968075 23.51665118436761 96-97 22.542366693847168 26.046454753508158 27.803813196285017 23.607365356359654 98-99 22.28644062184802 26.447276083756154 27.23121611810945 24.035067176286372 100-101 22.707404660174333 25.7154488316583 27.758648341208936 23.81849816695843 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 172.0 1 138.0 2 153.5 3 207.5 4 183.5 5 194.5 6 362.0 7 734.0 8 1184.5 9 1423.5 10 1335.0 11 1220.5 12 1285.0 13 1465.0 14 1640.0 15 1770.5 16 2065.0 17 2397.0 18 2692.5 19 3167.5 20 3766.5 21 4584.0 22 5757.5 23 7267.0 24 9227.5 25 12179.5 26 15961.5 27 20443.0 28 25235.5 29 30087.0 30 35215.0 31 43921.5 32 56857.0 33 61461.0 34 60561.0 35 64607.0 36 70702.5 37 77222.0 38 82495.0 39 86922.5 40 92546.0 41 99183.0 42 103148.5 43 105504.5 44 107898.0 45 113526.5 46 117228.0 47 118883.5 48 121424.0 49 118592.5 50 113582.0 51 109589.0 52 106302.0 53 105046.5 54 105256.0 55 99047.5 56 90019.0 57 82162.0 58 76152.0 59 67005.5 60 52376.5 61 43341.5 62 38054.5 63 28971.5 64 19927.5 65 14985.0 66 11621.5 67 9185.0 68 7227.5 69 5514.0 70 4179.0 71 2948.0 72 2076.5 73 1583.0 74 1195.0 75 908.5 76 701.0 77 470.0 78 310.0 79 210.5 80 128.0 81 78.5 82 45.5 83 24.5 84 19.5 85 17.0 86 9.0 87 6.5 88 8.0 89 7.5 90 5.0 91 3.0 92 1.5 93 0.5 94 1.0 95 0.5 96 0.0 97 1.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18179064267465095 2 0.04731271833689174 3 0.04647303869405271 4 0.051930956372506436 5 0.058390030548191314 6 0.06397712971015874 7 0.03546031722450999 8 0.034039320905859316 9 0.0393034663590425 10-11 0.040966677959281356 12-13 0.037317301050019396 14-15 0.03307045977950659 16-17 0.03155257734822064 18-19 0.038948217279379825 20-21 0.050300040143146 22-23 0.04663451554844483 24-25 0.06757806356310306 26-27 0.05212472859777698 28-29 0.04397014745097482 30-31 0.05333580500571789 32-33 0.0487175669701032 34-35 0.048572237801150296 36-37 0.045827131276484225 38-39 0.025448752252198423 40-41 0.024027755933547754 42-43 0.020604446620434768 44-45 0.004279136641391233 46-47 0.011545595089036723 48-49 0.010964278413225084 50-51 0.00910729458771568 52-53 0.00997926960143314 54-55 0.01152944740359751 56-57 0.008832783935249073 58-59 0.011497152032719086 60-61 0.011045016840421145 62-63 0.00403692135980305 64-65 0.007444082987476824 66-67 0.003827001449093291 68-69 0.00925262375666859 70-71 0.022364544333308894 72-73 0.019635585494082033 74-75 0.020071573000940764 76-77 0.012595194642585515 78-79 0.004537499608418628 80-81 0.00639448343392803 82-83 0.005296440824061601 84-85 0.005296440824061601 86-87 0.00829991031575507 88-89 0.02864599396916244 90-91 0.03536343111187472 92-93 0.022122329051720714 94-95 0.030632159278185543 96-97 0.03432997924376514 98-99 0.02087895727290137 100-101 0.12094616393969937 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3096419.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.052620529312435 #Duplication Level Percentage of deduplicated Percentage of total 1 75.68038057464999 40.150425121408546 2 13.245191765152766 14.053842651092474 3 4.7041308091330025 7.48699400211542 4 2.1881066074155253 4.643391580835884 5 1.1844910203050454 3.142017631031084 6 0.7122828659176179 2.267308355703511 7 0.4733883985034545 1.7580146548147897 8 0.3205330633418861 1.360409518126012 9 0.237102509568688 1.132101852002575 >10 1.1302353448673728 10.574155566837904 >50 0.07516928010535065 2.7730627397186147 >100 0.04089498999346572 4.156759164795799 >500 0.00410769439489489 1.4269465219448632 >1k 0.00367853229313473 4.120681723392527 >5k 3.0654435776122745E-4 0.9538889161800364 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6929 0.22377462481660265 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 5923 0.1912854817129077 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5626 0.18169375656201567 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5545 0.1790778315208633 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5304 0.171294647139163 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4776 0.15424269131535492 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4304 0.1389992762607386 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4227 0.13651253270309993 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4098 0.13234642985978318 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3966 0.12808344090383117 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3796 0.12259322785449901 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3720 0.12013877966773877 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3570 0.1152944740359751 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3213 0.10376502663237759 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.22953708784244E-5 0.0 5 0.0 0.0 0.0 3.22953708784244E-5 9.68861126352732E-5 6 0.0 0.0 0.0 3.22953708784244E-5 9.68861126352732E-5 7 3.22953708784244E-5 0.0 0.0 3.22953708784244E-5 9.68861126352732E-5 8 3.22953708784244E-5 0.0 0.0 3.22953708784244E-5 9.68861126352732E-5 9 3.22953708784244E-5 0.0 0.0 3.22953708784244E-5 9.68861126352732E-5 10-11 3.22953708784244E-5 0.0 0.0 3.22953708784244E-5 1.453291689529098E-4 12-13 3.22953708784244E-5 0.0 0.0 3.22953708784244E-5 5.328736194940027E-4 14-15 6.45907417568488E-5 0.0 0.0 3.22953708784244E-5 6.620551030077002E-4 16-17 6.45907417568488E-5 0.0 0.0 6.45907417568488E-5 7.104981593253368E-4 18-19 6.45907417568488E-5 0.0 0.0 6.45907417568488E-5 7.26645844764549E-4 20-21 6.45907417568488E-5 0.0 0.0 1.291814835136976E-4 8.396796428390344E-4 22-23 6.45907417568488E-5 0.0 0.0 2.583629670273952E-4 8.558273282782466E-4 24-25 1.291814835136976E-4 0.0 0.0 4.5213519229794156E-4 8.719750137174588E-4 26-27 1.291814835136976E-4 0.0 0.0 8.235319573998221E-4 9.042703845958832E-4 28-29 1.291814835136976E-4 0.0 0.0 0.0026966634683484375 9.042703845958832E-4 30-31 1.291814835136976E-4 0.0 0.0 0.010770506187954536 9.042703845958832E-4 32-33 1.291814835136976E-4 0.0 0.0 0.031617168089977485 9.365657554743076E-4 34-35 1.291814835136976E-4 0.0 0.0 0.06213629357008854 9.68861126352732E-4 36-37 1.291814835136976E-4 0.0 0.0 0.112775435107458 9.68861126352732E-4 38-39 1.61476854392122E-4 0.0 0.0 0.1908333465206098 0.0010011564972311564 40-41 1.61476854392122E-4 0.0 0.0 0.27413925570150555 0.0010011564972311564 42-43 1.7762453983133418E-4 0.0 0.0 0.36098150799358875 0.0010011564972311564 44-45 2.260675961489708E-4 0.0 0.0 0.4729172634582077 0.001049599553548793 46-47 2.583629670273952E-4 0.0 0.0 0.6029384266147444 0.0011141902953056416 48-49 2.583629670273952E-4 0.0 0.0 0.7419699982463615 0.001130337980744854 50-51 2.583629670273952E-4 0.0 0.0 0.8951146469518498 0.001130337980744854 52-53 2.583629670273952E-4 0.0 0.0 1.0398786469143873 0.0011626333516232784 54-55 2.583629670273952E-4 0.0 0.0 1.19990220961698 0.0011949287225017028 56-57 2.906583379058196E-4 0.0 0.0 1.3850031278066695 0.0012433717788193394 58-59 2.906583379058196E-4 0.0 0.0 1.5833774434273913 0.0012595194642585515 60-61 2.906583379058196E-4 0.0 0.0 1.801823332049054 0.0012595194642585515 62-63 2.906583379058196E-4 0.0 0.0 2.0101284742148913 0.0013241102060154004 64-65 2.906583379058196E-4 0.0 0.0 2.23480736941609 0.0013402578914546125 66-67 2.906583379058196E-4 0.0 0.0 2.500291465722178 0.0013564055768938248 68-69 3.068060233450318E-4 0.0 0.0 2.7915311203038087 0.0013564055768938248 70-71 3.22953708784244E-4 3.22953708784244E-5 0.0 3.09979043533837 0.0013725532623330371 72-73 3.391013942234562E-4 3.22953708784244E-5 0.0 3.414315052323345 0.0015663254876035833 74-75 3.552490796626684E-4 3.22953708784244E-5 0.0 3.7317301050019394 0.00176009771287413 76-77 3.552490796626684E-4 3.22953708784244E-5 0.0 4.079648135475205 0.0018085407691917665 78-79 3.552490796626684E-4 3.22953708784244E-5 0.0 4.467515539725083 0.0018408361400701907 80-81 4.521351922979416E-4 3.22953708784244E-5 0.0 4.874727871131135 0.001856983825509403 82-83 4.521351922979416E-4 6.45907417568488E-5 0.0 5.299185930586267 0.001970017623583888 84-85 4.521351922979416E-4 6.45907417568488E-5 0.0 5.7322507063804995 0.0019861653090231003 86-87 4.521351922979416E-4 6.45907417568488E-5 0.0 6.1819475981771195 0.002099199107097586 88-89 4.521351922979416E-4 9.68861126352732E-5 0.0 6.653476160687555 0.002163789848854435 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6755 0.0 32.7738 1 GTATCAA 10580 0.0 32.580074 1 ATCAACG 12895 0.0 26.465836 3 TATCAAC 13010 0.0 26.304962 2 TCAACGC 12990 0.0 26.199099 4 CAACGCA 13010 0.0 26.1206 5 GTGTAAG 1450 0.0 25.610956 1 AGGTAAG 2170 0.0 25.4086 2 AACGCAG 13530 0.0 25.251093 6 TAAGGTG 2300 0.0 25.210787 5 GTACATG 9420 0.0 24.209385 1 TAAGGTA 1220 0.0 24.155405 4 TACATGG 9365 0.0 23.803867 2 GTAAGGT 2515 0.0 22.679094 4 GGTAAGG 2475 0.0 22.661533 3 ACATGGG 9480 0.0 22.61261 3 ACGCAGA 15020 0.0 22.552334 7 CGCAGAG 15245 0.0 22.188322 8 GTATAGG 895 0.0 21.810217 1 AAGGTAA 2340 0.0 21.770433 1 >>END_MODULE