FastQCFastQC Report
Thu 26 May 2016
SRR938474_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938474_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3096419
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70010.22609989151984922No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA59200.19118859560027243No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57110.18443886308668175No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA56900.18376066029823485No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA56830.18353459270208586No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA49740.16063717474928296No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC49720.1605725840075261No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG45760.14778361713967006No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA39150.1264363769890315No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38940.1257581742005846No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA38920.12569358345882775No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA38730.1250799714121377No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG36650.11836253426942543No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA33780.10909376282731763No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT32230.10408798034116185No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT31380.10134287381649576No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65350.034.4259221
GTATCAA101300.032.1626051
TAAGGTG23500.025.89545
ATCAACG126250.025.418673
TATCAAC127050.025.3819542
TCAACGC127050.025.1837774
CAACGCA128500.024.9735985
AGGTAAG22250.023.941872
AACGCAG134150.023.8632016
GTGTAAG12800.023.7815441
GTAAGGT26250.023.0014324
GTACATG89900.022.485331
GGTAAGG25650.022.0566773
TACATGG89950.021.8383062
AAGGTAA23800.021.7830941
ACATGGG88200.021.3994393
ACGCAGA150250.021.2429267
CGCAGAG152100.021.0157728
AGAGTAC130350.020.53252610-11
GAGTACT93050.019.49873712-13
Produced by FastQC (version 0.11.5)