##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938474_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3096419 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.742842619167497 31.0 31.0 34.0 30.0 34.0 2 31.968172911999314 31.0 31.0 34.0 30.0 34.0 3 32.03511540266353 33.0 31.0 34.0 30.0 34.0 4 35.72074063619943 37.0 35.0 37.0 33.0 37.0 5 35.58250611432109 37.0 35.0 37.0 33.0 37.0 6 35.60935196431749 37.0 35.0 37.0 33.0 37.0 7 35.54304827608925 37.0 35.0 37.0 33.0 37.0 8 35.52362939253376 37.0 35.0 37.0 33.0 37.0 9 37.10101313807983 39.0 37.0 39.0 33.0 39.0 10-11 37.00534294615812 39.0 37.0 39.0 33.0 39.0 12-13 36.90418738549272 39.0 37.0 39.0 33.0 39.0 14-15 37.95552685214759 40.0 37.0 41.0 33.0 41.0 16-17 37.87914975331181 40.0 37.0 41.0 33.0 41.0 18-19 37.98432592617472 40.0 37.0 41.0 33.0 41.0 20-21 38.019514800806995 40.0 37.0 41.0 33.0 41.0 22-23 37.93410840070417 40.0 37.0 41.0 32.5 41.0 24-25 37.83625052035916 40.0 37.0 41.0 32.0 41.0 26-27 37.64930908898312 40.0 37.0 41.0 32.0 41.0 28-29 37.52918597257025 39.5 36.5 41.0 32.0 41.0 30-31 37.28688591563351 39.0 36.0 41.0 31.0 41.0 32-33 37.06941502425867 39.0 36.0 41.0 31.0 41.0 34-35 36.92084226973158 39.0 36.0 40.5 30.0 41.0 36-37 36.91048869678167 39.0 36.0 41.0 30.0 41.0 38-39 36.77927099013409 39.0 35.0 41.0 30.0 41.0 40-41 36.60638498859489 39.0 35.0 40.5 30.0 41.0 42-43 36.39077883193457 39.0 35.0 40.0 30.0 41.0 44-45 36.13574051186225 39.0 35.0 40.0 29.5 41.0 46-47 35.797493330198535 38.0 35.0 40.0 28.5 41.0 48-49 35.44949230062211 38.0 34.5 40.0 27.0 41.0 50-51 35.07918598871794 38.0 34.0 40.0 26.5 41.0 52-53 34.58430060660395 38.0 33.0 40.0 25.5 41.0 54-55 34.27240564019275 38.0 33.0 40.0 24.0 41.0 56-57 33.93864476997461 37.0 33.0 40.0 24.0 41.0 58-59 33.501163279259046 37.0 32.0 39.5 23.0 41.0 60-61 33.12989860222405 36.0 32.0 39.0 22.0 40.5 62-63 32.67421560195827 36.0 31.0 39.0 21.0 40.0 64-65 32.18164757418165 35.0 31.0 38.5 19.5 40.0 66-67 31.69270583212414 35.0 30.0 38.0 18.0 40.0 68-69 31.16541204533366 34.5 29.5 38.0 15.5 40.0 70-71 30.578816852628794 34.0 29.0 37.0 12.0 39.0 72-73 30.141053261848608 34.0 29.0 36.0 8.0 39.0 74-75 29.63886880296239 34.0 28.0 36.0 7.5 39.0 76-77 28.385511618421152 31.5 26.5 34.5 7.0 36.5 78-79 29.077966999944127 33.5 28.0 35.0 4.0 37.0 80-81 28.81301932974833 33.0 28.0 35.0 2.0 37.0 82-83 28.338767460088572 33.0 27.0 35.0 2.0 36.0 84-85 27.813340022781155 33.0 26.0 35.0 2.0 36.0 86-87 27.24645824741419 32.5 25.5 35.0 2.0 35.5 88-89 26.59428455903416 32.0 25.0 34.0 2.0 35.0 90-91 26.053751446428922 32.0 24.0 34.0 2.0 35.0 92-93 25.329788055169537 31.0 21.0 34.0 2.0 35.0 94-95 24.55857944289839 31.0 18.5 34.0 2.0 35.0 96-97 23.564313324521002 31.0 4.5 34.0 2.0 35.0 98-99 22.332039203996615 30.0 2.0 34.0 2.0 35.0 100-101 18.67037196839317 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28001724510915693 1101 2 0.3335756625383546 1101 3 0.31329830498076916 1101 4 0.2852766046418793 1101 5 0.1168448847132666 1101 6 0.22703319554575785 1101 7 0.26431712434763455 1101 8 0.30388922639705385 1101 9 0.29630832185622324 1101 10-11 0.3083685230969877 1101 12-13 0.3005217957762554 1101 14-15 0.3918413317018974 1101 16-17 0.424545278950923 1101 18-19 0.4095603350904966 1101 20-21 0.3966529257243039 1101 22-23 0.45685337810117943 1101 24-25 0.3771318619803381 1101 26-27 0.5041001938286414 1101 28-29 0.45544087323966664 1101 30-31 0.4054545917765111 1101 32-33 0.35524285085716656 1101 34-35 0.21591739933176513 1101 36-37 0.4044368809336376 1101 38-39 0.4536704557646374 1101 40-41 0.381030988474528 1101 42-43 0.5002361091431453 1101 44-45 0.5168051203129806 1101 46-47 0.4940932126063231 1101 48-49 0.5717946315209019 1101 50-51 0.5819483041433386 1101 52-53 0.7096423384661037 1101 54-55 0.6929383619580918 1101 56-57 0.6499548407066058 1101 58-59 0.681614025768944 1101 60-61 0.7785541406497387 1101 62-63 0.7469178589819379 1101 64-65 0.6485040180776807 1101 66-67 0.5409853479902047 1101 68-69 0.5739766568954074 1101 70-71 0.4116326887707409 1101 72-73 0.36002193321002096 1101 74-75 0.43934274941063123 1101 76-77 0.32693002187656717 1101 78-79 0.38868592036783056 1101 80-81 0.5390640663583071 1101 82-83 0.4854580775784285 1101 84-85 0.5669310585669542 1101 86-87 0.6303700429341923 1101 88-89 0.6420243821768814 1101 90-91 0.6282425671877938 1101 92-93 0.5078484228212581 1101 94-95 0.4556242233754162 1101 96-97 0.623447252350875 1101 98-99 0.4090288586732882 1101 100-101 0.39979377123407644 1102 1 0.3323371755368214 1102 2 0.2947879491036183 1102 3 0.3174267940181963 1102 4 0.2591685060583373 1102 5 0.09942195994688063 1102 6 0.2470385574658991 1102 7 0.3029878418218104 1102 8 0.24128028279005775 1102 9 0.3344769514024861 1102 10-11 0.3350471546137186 1102 12-13 0.28592264193651573 1102 14-15 0.40691178505887393 1102 16-17 0.46049856393224076 1102 18-19 0.4390928154427556 1102 20-21 0.3472981560784518 1102 22-23 0.4430625384585838 1102 24-25 0.3659146966361604 1102 26-27 0.3969551036801775 1102 28-29 0.3535703115074824 1102 30-31 0.20672866357698894 1102 32-33 0.22476725043578938 1102 34-35 0.18043541386767714 1102 36-37 0.25587901008960046 1102 38-39 0.3022315001833178 1102 40-41 0.35510169950682524 1102 42-43 0.27142350919922364 1102 44-45 0.36026784075799867 1102 46-47 0.20027869904426865 1102 48-49 0.3077281171858175 1102 50-51 0.2972440812996666 1102 52-53 0.20799823733612044 1102 54-55 0.24402083511059658 1102 56-57 0.44133328048257425 1102 58-59 0.3830171246935308 1102 60-61 0.31019728497064847 1102 62-63 0.3355756926904121 1102 64-65 0.280503881661339 1102 66-67 0.4888688579069118 1102 68-69 0.28198860658809366 1102 70-71 0.25657159509060534 1102 72-73 0.2141419435928178 1102 74-75 0.22931179047953165 1102 76-77 0.17105208797786275 1102 78-79 0.19968391959480414 1102 80-81 0.16926991293539828 1102 82-83 -0.021149352186103698 1102 84-85 -0.05703066149155234 1102 86-87 -0.022055325958330485 1102 88-89 0.03798868467254479 1102 90-91 -0.16271265035837956 1102 92-93 -0.06020604329822632 1102 94-95 -0.02693343147607763 1102 96-97 -0.07916441010961606 1102 98-99 -0.20652028341948636 1102 100-101 -0.2958270273320345 1103 1 0.3004035696901681 1103 2 0.3323404216802466 1103 3 0.29700458188906254 1103 4 0.20045496159599452 1103 5 0.23427476146634518 1103 6 0.11622299025741256 1103 7 0.3117050564971535 1103 8 0.2701540779279412 1103 9 0.3945721016747683 1103 10-11 0.4377451052585286 1103 12-13 0.37388824286507827 1103 14-15 0.5200498859989082 1103 16-17 0.5269021222037509 1103 18-19 0.5189076761352283 1103 20-21 0.46853687590814985 1103 22-23 0.5191422692484622 1103 24-25 0.43991330195520817 1103 26-27 0.5061754816203674 1103 28-29 0.594769828874476 1103 30-31 0.6160804810205889 1103 32-33 0.6345758947049802 1103 34-35 0.618730875190515 1103 36-37 0.608244462374536 1103 38-39 0.7206934744826512 1103 40-41 0.6978650716985371 1103 42-43 0.6894426493600747 1103 44-45 0.7432517009962822 1103 46-47 0.74707425900408 1103 48-49 0.8181376441412382 1103 50-51 0.7839455232345998 1103 52-53 0.7995310559220812 1103 54-55 0.8096592686636868 1103 56-57 0.8050885506790664 1103 58-59 0.8169216349265369 1103 60-61 0.9437132114012314 1103 62-63 0.7772433916044861 1103 64-65 0.7217597138592069 1103 66-67 0.857367649030504 1103 68-69 0.892587149513524 1103 70-71 0.45698609977241844 1103 72-73 0.573746202875018 1103 74-75 0.67468846902851 1103 76-77 0.47409113904429034 1103 78-79 0.6273447093328883 1103 80-81 0.5624577682005452 1103 82-83 0.6222620419901865 1103 84-85 0.5719075917623222 1103 86-87 0.6796609574004613 1103 88-89 0.5303086492549873 1103 90-91 0.7009517820661166 1103 92-93 0.5281494030684932 1103 94-95 0.5017281794721633 1103 96-97 0.27530900607877484 1103 98-99 0.37835190141244013 1103 100-101 0.07067264464267353 1104 1 0.27427094203772384 1104 2 0.2684446523784523 1104 3 0.29322939311322926 1104 4 0.22840547506843478 1104 5 0.20606628258815363 1104 6 0.149450376765202 1104 7 0.1601196236211493 1104 8 0.2739371335014056 1104 9 0.34121937085801335 1104 10-11 0.34505844090091387 1104 12-13 0.32956889021511415 1104 14-15 0.593180729417945 1104 16-17 0.5360049904163233 1104 18-19 0.5392443860000853 1104 20-21 0.5067631206376717 1104 22-23 0.48931295690490373 1104 24-25 0.476208521018215 1104 26-27 0.5987704213027811 1104 28-29 0.6034888629191357 1104 30-31 0.5930159590760482 1104 32-33 0.6568268040303025 1104 34-35 0.6284165419944898 1104 36-37 0.7188417489849215 1104 38-39 0.7322013429191969 1104 40-41 0.7177572528665124 1104 42-43 0.7844015071010659 1104 44-45 0.8034949817131292 1104 46-47 0.7467734632556429 1104 48-49 0.7112022971990726 1104 50-51 0.8608181215395234 1104 52-53 0.8915103094285399 1104 54-55 0.8346865019874414 1104 56-57 0.9199045714124523 1104 58-59 0.8971985899076955 1104 60-61 0.8764352654220957 1104 62-63 0.8423414151808473 1104 64-65 0.8342752425899462 1104 66-67 0.8007398720236303 1104 68-69 0.6502241164697011 1104 70-71 0.5600548610467975 1104 72-73 0.5816619494723554 1104 74-75 0.6538266504215677 1104 76-77 0.7781451620918212 1104 78-79 0.7708090394149743 1104 80-81 0.7808925704762331 1104 82-83 0.644489460183685 1104 84-85 0.5727634337882854 1104 86-87 0.593242601102272 1104 88-89 0.5943155370733599 1104 90-91 0.5528555470936141 1104 92-93 0.5331060865140209 1104 94-95 0.503765516167828 1104 96-97 0.5898050557444883 1104 98-99 0.47411683275824146 1104 100-101 0.35767532918736933 1105 1 0.24118530260126647 1105 2 0.2604586060561651 1105 3 0.24385313250792962 1105 4 0.17593278245786337 1105 5 0.1302510179739116 1105 6 0.19109516590510367 1105 7 0.27255833296795373 1105 8 0.24653624153888387 1105 9 0.29839128389507863 1105 10-11 0.3167404492155086 1105 12-13 0.31360020705522373 1105 14-15 0.34603942735434856 1105 16-17 0.2458827428261543 1105 18-19 0.3866799679195694 1105 20-21 0.40346312173284105 1105 22-23 0.34534250659876875 1105 24-25 0.39231747864369027 1105 26-27 0.28508166055541295 1105 28-29 0.3458344148124226 1105 30-31 0.33018642979777013 1105 32-33 0.4828174504706624 1105 34-35 0.4286654240451213 1105 36-37 0.42597698423212904 1105 38-39 0.40081570354472973 1105 40-41 0.4236273109992652 1105 42-43 0.509994710944234 1105 44-45 0.3409974019306645 1105 46-47 0.2494222712481431 1105 48-49 0.1923231539261181 1105 50-51 0.1678031638202384 1105 52-53 0.2535819825825243 1105 54-55 0.3512942568913644 1105 56-57 0.37861447199807685 1105 58-59 0.4471662411302262 1105 60-61 0.3917925143274914 1105 62-63 0.5953679010911017 1105 64-65 0.2404946282586664 1105 66-67 0.16891912041202417 1105 68-69 0.02084124972951429 1105 70-71 0.1958030290686814 1105 72-73 0.24000787206961505 1105 74-75 0.17660123327111776 1105 76-77 0.2028842486929392 1105 78-79 0.2116059723665309 1105 80-81 0.3358057825600298 1105 82-83 0.40391012467778964 1105 84-85 0.3169681880150641 1105 86-87 0.44761147345757024 1105 88-89 0.33949571387477206 1105 90-91 0.37142472688111283 1105 92-93 0.5055578634857802 1105 94-95 0.5130761472923702 1105 96-97 0.4203328997661444 1105 98-99 0.384291582279932 1105 100-101 -0.08365730613146383 1106 1 0.15570719091961394 1106 2 0.11267209167714398 1106 3 0.12082306095132367 1106 4 0.08510560751903995 1106 5 -0.029328089356951637 1106 6 0.05962965998718772 1106 7 0.18637354677892404 1106 8 0.14643176606838892 1106 9 0.06560760874305771 1106 10-11 0.09923120909773786 1106 12-13 0.08833896785893813 1106 14-15 0.1157907474433415 1106 16-17 -0.03538270368018459 1106 18-19 0.2003799960087136 1106 20-21 0.13722910880374428 1106 22-23 0.2871233991062425 1106 24-25 0.16107810048492155 1106 26-27 0.05335243995859429 1106 28-29 0.23585993754341672 1106 30-31 0.20222065006115741 1106 32-33 0.20937634775935265 1106 34-35 0.1311423212246865 1106 36-37 -0.2738310395648327 1106 38-39 0.019877121784084295 1106 40-41 0.23903905953873306 1106 42-43 0.3592472162336833 1106 44-45 0.21180075161228729 1106 46-47 -0.13016510116346325 1106 48-49 0.06330898204073065 1106 50-51 0.0576956167304985 1106 52-53 -0.02080243056642672 1106 54-55 -0.2865533463362624 1106 56-57 0.043554363238506255 1106 58-59 0.18995581702447595 1106 60-61 -0.21817893173830782 1106 62-63 -0.15839937929317216 1106 64-65 -0.48922258069025304 1106 66-67 -0.10400465695215999 1106 68-69 -1.0140152632147377 1106 70-71 -0.7666421819333742 1106 72-73 -0.5772517989842143 1106 74-75 -0.2520209029996572 1106 76-77 -0.2523890344840858 1106 78-79 -0.28202304473552076 1106 80-81 -0.9584730234692387 1106 82-83 -0.5783844153169184 1106 84-85 -0.5370391636631133 1106 86-87 -0.25970241571760155 1106 88-89 -0.1755815919109871 1106 90-91 -0.2703444449739614 1106 92-93 -0.19228826455628578 1106 94-95 -0.1728473491803193 1106 96-97 -0.11972930052364106 1106 98-99 -0.4640743971119292 1106 100-101 -0.8166867108233618 1107 1 0.11663220017519293 1107 2 0.11109834970109134 1107 3 0.09368081105269255 1107 4 0.117465064760232 1107 5 0.07097239859317028 1107 6 0.1145384460276162 1107 7 0.1349111655588331 1107 8 0.10824589840453314 1107 9 0.2169352209216342 1107 10-11 0.083490067517765 1107 12-13 0.14969355343433932 1107 14-15 0.20465604304395413 1107 16-17 0.232414057553342 1107 18-19 0.21864551060902215 1107 20-21 0.20794700860061965 1107 22-23 0.15545306612093412 1107 24-25 0.22506890954971226 1107 26-27 0.2382473377358849 1107 28-29 0.2496320407435917 1107 30-31 0.21403155029093313 1107 32-33 0.21715475454638522 1107 34-35 0.26462429880366045 1107 36-37 0.30990470733576814 1107 38-39 0.3437358454350772 1107 40-41 0.3375424963451721 1107 42-43 0.31561593492877194 1107 44-45 0.38200513826623705 1107 46-47 0.3224300843692376 1107 48-49 0.32195447481942807 1107 50-51 0.27599658655829273 1107 52-53 0.3550948707226951 1107 54-55 0.289965890573221 1107 56-57 0.3678639167572939 1107 58-59 0.2759222070358973 1107 60-61 0.21583702899062018 1107 62-63 0.427153986700759 1107 64-65 0.29363961600594024 1107 66-67 0.23662243044987008 1107 68-69 0.33985650639288423 1107 70-71 0.3240348562035251 1107 72-73 0.22500822691181455 1107 74-75 0.22366523400639338 1107 76-77 0.14715288789443193 1107 78-79 0.379312539972112 1107 80-81 0.51689930539073 1107 82-83 0.3863805321956342 1107 84-85 0.2812951066545928 1107 86-87 0.3225084625730297 1107 88-89 0.2861273500540982 1107 90-91 0.17462972755783213 1107 92-93 0.02410321600308407 1107 94-95 0.06643733670791363 1107 96-97 0.27463498332973657 1107 98-99 -0.04297168077138025 1107 100-101 -0.5745543433348281 1108 1 0.2055352745806971 1108 2 0.19534929306805893 1108 3 0.1886395043523379 1108 4 0.1470486565193383 1108 5 0.11570162475849344 1108 6 0.1384050275064368 1108 7 0.1718006305889901 1108 8 0.15700479131881195 1108 9 0.2273566858673277 1108 10-11 0.18921327254173548 1108 12-13 0.1905341997731469 1108 14-15 0.31844899957366835 1108 16-17 0.2968146315435334 1108 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-0.6767218038142957 2316 62-63 -0.6722341532405878 2316 64-65 -0.6677619554891834 2316 66-67 -0.5401737473992831 2316 68-69 -0.6442524114461463 2316 70-71 -0.7047794568203152 2316 72-73 -0.5152905318106029 2316 74-75 -0.3008456923176972 2316 76-77 -0.3796085736357142 2316 78-79 -0.3074489734702901 2316 80-81 -0.521306629403405 2316 82-83 -0.2746847406023285 2316 84-85 -0.32845972475387697 2316 86-87 -0.19894877183508086 2316 88-89 0.0132779866188244 2316 90-91 -0.0988188979447635 2316 92-93 -0.06653386228633806 2316 94-95 -0.20035956791277698 2316 96-97 -0.09606007723795429 2316 98-99 0.0432763092510875 2316 100-101 0.20258584588904327 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11.0 3 16.0 4 46.0 5 91.0 6 182.0 7 555.0 8 1421.0 9 2554.0 10 4036.0 11 6261.0 12 9064.0 13 12164.0 14 16083.0 15 20650.0 16 25190.0 17 30809.0 18 29404.0 19 26407.0 20 26202.0 21 27593.0 22 30543.0 23 34186.0 24 39735.0 25 47142.0 26 56006.0 27 68181.0 28 81422.0 29 96591.0 30 115615.0 31 137530.0 32 164846.0 33 202908.0 34 257080.0 35 334035.0 36 436290.0 37 491031.0 38 252271.0 39 12267.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.91580332341534 19.28017535241812 15.079513485529777 23.724507838636764 2 15.77349536330248 20.4845067000368 39.221217047072635 24.520780889588085 3 19.30890600197972 23.544089174669253 30.292679067108686 26.854325756242343 4 13.30020258886152 16.971281987353777 36.48230423595773 33.246211187826965 5 13.519511684254896 37.35056902075678 34.70974898957119 14.420170305417141 6 32.904041733370065 36.39355655678382 16.734556918814928 13.967844791031188 7 28.811507744914366 32.592875834956445 20.329031697583563 18.266584722545627 8 26.53481327946896 35.15218709095894 19.51066699952429 18.802332630047808 9 26.05703556269355 16.742663056905414 19.911000416933238 37.289300963467795 10-11 24.97667466838306 26.448778411448835 27.480906169352405 21.093640750815702 12-13 25.99160514129386 23.64329569092555 27.535113949371837 22.829985218408748 14-15 22.218246303229634 25.371808531080582 25.766506406271244 26.64343875941854 16-17 22.11033132143938 28.44912138828757 26.99340754594259 22.447139744330467 18-19 22.22076534215815 26.869845456961734 29.025125475589707 21.88426372529041 20-21 22.487831911637283 26.123983866524526 29.321080254319586 22.06710396751861 22-23 22.235023748400977 26.17836927108379 29.217654329081434 22.3689526514338 24-25 22.288365096176655 26.404904516082567 29.21375275863689 22.09297762910388 26-27 21.701868930980524 26.771931637789454 28.91882027870052 22.607379152529507 28-29 21.964469278866975 27.31164613057858 28.674995212211268 22.048889378343176 30-31 22.00947287818606 26.675168961306596 28.611599399176917 22.703758761330427 32-33 21.58373915158123 26.65516197904741 28.677481955768908 23.083616913602455 34-35 22.62355194678202 26.822629721005143 28.376037374804046 22.17778095740879 36-37 22.819276485871566 26.130218333136867 28.510325650792932 22.540179530198635 38-39 22.051821323111216 26.634301081971245 28.51792449874737 22.795953096170162 40-41 22.663381615426154 26.443149902720968 28.60202089755084 22.29144758430203 42-43 22.31564047256031 26.53010309684541 28.32989763303233 22.82435879756195 44-45 22.30756275354898 26.715461750406156 28.69101834910236 22.285957146942508 46-47 22.882788149794973 27.0382981114636 27.69266691620223 22.386246822539196 48-49 22.34855812472408 27.17758804606224 27.68467381190982 22.78918001730386 50-51 22.61719101969081 27.25411192735867 27.774777896660623 22.353919156289894 52-53 23.086152100216413 26.8459468825117 27.749167667554037 22.31873334971785 54-55 22.25629025012442 27.178040181254538 28.043362348571044 22.52230722005 56-57 22.49829561180189 27.30152153180819 27.598865011485852 22.601317844904063 58-59 22.37465278439384 26.845575485746597 27.68199329612691 23.097778433732643 60-61 21.839841442647135 27.223431325024166 28.15855993649438 22.77816729583432 62-63 22.101611571302204 27.13928573620043 28.235099965476245 22.524002727021116 64-65 21.727437404304776 27.27143839383494 27.951546609163678 23.049577592696597 66-67 21.652707250461944 27.289949527138873 27.88093914368432 23.176404078714864 68-69 22.539662598447002 26.75804591752453 27.91716637339594 22.785125110632524 70-71 22.18195347010643 26.988604251751543 27.447311700164512 23.382130577977524 72-73 22.106504319990286 26.988127252803967 27.66657225653247 23.238796170673286 74-75 22.16683207279118 27.439939491393122 27.41016315944321 22.98306527637248 76-77 22.185482649473474 26.888932021150886 27.776796357340526 23.148788972035113 78-79 22.321510751613395 27.170273144558276 27.6171280437176 22.89108806011073 80-81 22.65017412217909 27.136981173877057 27.232834763332388 22.980009940611467 82-83 22.384742654123485 27.2660773150213 27.466616845320544 22.88256318553467 84-85 22.250516063407126 27.38991495107107 27.45882029592641 22.90074868959539 86-87 22.60023224144972 27.294894159939787 27.04267755562887 23.062196042981622 88-89 22.28384179980839 27.51155891879602 26.787706506727044 23.41689277466854 90-91 22.108732971969864 27.47877042116171 27.031224238642636 23.381272368225787 92-93 22.397783608592537 27.05610365896096 27.286565993338357 23.259546739108146 94-95 21.99342563028867 26.76876526864828 27.554075781422956 23.683733319640094 96-97 22.33732294261903 26.290018613440015 27.603374673029503 23.76928377091146 98-99 21.99685959610737 26.71663732630327 27.114564995606212 24.171938081983143 100-101 22.02996784833833 26.349861945341047 27.596253542600913 24.023916663719714 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 163.0 1 129.5 2 138.5 3 184.0 4 173.0 5 180.5 6 315.5 7 742.5 8 1203.0 9 1348.5 10 1265.5 11 1127.0 12 1180.5 13 1373.5 14 1540.5 15 1677.5 16 1877.0 17 2198.0 18 2552.5 19 3046.0 20 3670.0 21 4431.0 22 5502.0 23 6932.0 24 9017.0 25 11707.5 26 15379.5 27 20336.0 28 25170.0 29 30075.5 30 35694.5 31 44490.5 32 58267.5 33 62922.0 34 61139.5 35 64908.5 36 71543.0 37 78302.5 38 82585.5 39 86708.0 40 92510.5 41 99590.0 42 103481.5 43 105756.5 44 108413.5 45 114114.5 46 117086.5 47 119333.0 48 122610.5 49 118624.0 50 113728.5 51 110061.0 52 106788.0 53 106995.5 54 108493.0 55 101275.5 56 91267.0 57 83088.5 58 76009.0 59 66099.0 60 51433.0 61 42756.0 62 37511.5 63 27300.0 64 17594.5 65 12993.0 66 9933.5 67 7986.5 68 6214.0 69 4669.5 70 3534.0 71 2305.0 72 1534.0 73 1205.0 74 921.5 75 705.0 76 522.0 77 337.5 78 209.5 79 128.5 80 80.5 81 43.5 82 23.0 83 12.5 84 11.0 85 7.5 86 2.5 87 1.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 8.396796428390344E-4 2 0.04637615258141743 3 7.750889010821856E-4 4 0.0 5 0.08664848006681267 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.4221528158818297E-4 26-27 0.008525977911904042 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010415257108291867 36-37 1.291814835136976E-4 38-39 0.004069216730681474 40-41 0.005522508420210573 42-43 3.22953708784244E-4 44-45 1.61476854392122E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 8.0738427196061E-5 68-69 6.297597321292757E-4 70-71 3.22953708784244E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 9.688611263527319E-4 82-83 7.104981593253368E-4 84-85 0.004602090350175477 86-87 0.002341414388685769 88-89 1.61476854392122E-5 90-91 0.0029227310644974083 92-93 0.0011141902953056418 94-95 1.61476854392122E-5 96-97 1.61476854392122E-5 98-99 6.45907417568488E-5 100-101 1.61476854392122E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3096419.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.99420733079496 #Duplication Level Percentage of deduplicated Percentage of total 1 73.0086352173287 36.50008845993509 2 14.685879598308176 14.684178189458214 3 5.1952452975725745 7.791965116235425 4 2.4093077062872306 4.818057159672233 5 1.3126176877831819 3.2811640414550545 6 0.778116485533908 2.3340790143175028 7 0.5344632320692976 1.8704045944331473 8 0.37152966085733347 1.4859464715553172 9 0.26040687541998 1.1716951788099874 >10 1.298753096595709 11.428431915709785 >50 0.08739480156104566 3.0196741880818725 >100 0.048594557583427574 4.677095926692045 >500 0.004592575715083667 1.525852025055433 >1k 0.004139786559442748 4.441055960232862 >5k 3.234208249564647E-4 0.9703117583559939 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7001 0.22609989151984922 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5920 0.19118859560027243 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5711 0.18443886308668175 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5690 0.18376066029823485 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 5683 0.18353459270208586 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4974 0.16063717474928296 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4972 0.1605725840075261 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4576 0.14778361713967006 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3915 0.1264363769890315 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3894 0.1257581742005846 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3892 0.12569358345882775 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3873 0.1250799714121377 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3665 0.11836253426942543 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3378 0.10909376282731763 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3223 0.10408798034116185 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3138 0.10134287381649576 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.22953708784244E-5 2 0.0 0.0 0.0 0.0 3.22953708784244E-5 3 0.0 0.0 0.0 0.0 3.22953708784244E-5 4 0.0 0.0 0.0 0.0 3.22953708784244E-5 5 0.0 0.0 0.0 3.22953708784244E-5 9.68861126352732E-5 6 0.0 0.0 0.0 3.22953708784244E-5 1.61476854392122E-4 7 3.22953708784244E-5 0.0 0.0 3.22953708784244E-5 1.61476854392122E-4 8 3.22953708784244E-5 0.0 0.0 3.22953708784244E-5 1.61476854392122E-4 9 3.22953708784244E-5 0.0 0.0 3.22953708784244E-5 1.61476854392122E-4 10-11 3.22953708784244E-5 0.0 0.0 3.22953708784244E-5 1.61476854392122E-4 12-13 6.45907417568488E-5 0.0 0.0 3.22953708784244E-5 3.875444505410928E-4 14-15 9.68861126352732E-5 0.0 0.0 3.22953708784244E-5 4.682828777371538E-4 16-17 9.68861126352732E-5 0.0 0.0 3.22953708784244E-5 5.167259340547904E-4 18-19 9.68861126352732E-5 0.0 0.0 9.68861126352732E-5 6.136120466900636E-4 20-21 9.68861126352732E-5 0.0 0.0 1.453291689529098E-4 7.912365865213977E-4 22-23 9.68861126352732E-5 0.0 0.0 2.42215281588183E-4 8.396796428390344E-4 24-25 1.61476854392122E-4 0.0 0.0 3.713967651018806E-4 8.396796428390344E-4 26-27 1.61476854392122E-4 0.0 0.0 7.427935302037612E-4 9.850088117919443E-4 28-29 1.937722252705464E-4 0.0 0.0 0.002745106524666074 0.0010334518681095809 30-31 1.937722252705464E-4 0.0 0.0 0.010883539986029022 0.001049599553548793 32-33 1.937722252705464E-4 0.0 0.0 0.03189167874244409 0.001065747238988005 34-35 2.260675961489708E-4 0.0 0.0 0.06297597321292758 0.001130337980744854 36-37 2.583629670273952E-4 0.0 0.0 0.11445479439313608 0.001130337980744854 38-39 3.22953708784244E-4 0.0 0.0 0.19322320396561318 0.0012110764079409152 40-41 3.22953708784244E-4 0.0 0.0 0.27720731593495584 0.0012272240933801273 42-43 3.713967651018806E-4 0.0 0.0 0.36492154324075654 0.0012272240933801273 44-45 3.875444505410928E-4 0.0 0.0 0.4772932862122342 0.0013402578914546125 46-47 4.198398214195172E-4 0.0 0.0 0.6081702766970492 0.0013887009477722492 48-49 4.198398214195172E-4 0.0 0.0 0.7477508696335993 0.0014209963186506737 50-51 4.198398214195172E-4 0.0 0.0 0.9017190502964876 0.0014532916895290979 52-53 4.198398214195172E-4 0.0 0.0 1.047855603521358 0.0015824731730427956 54-55 4.198398214195172E-4 0.0 0.0 1.2075077694588492 0.0016470639147996443 56-57 4.521351922979416E-4 0.0 0.0 1.393480662662256 0.0016632116002388564 58-59 4.521351922979416E-4 0.0 0.0 1.5930660546909188 0.0016793592856780687 60-61 4.521351922979416E-4 0.0 0.0 1.812690724349644 0.0017278023419957053 62-63 4.521351922979416E-4 0.0 0.0 2.02204546606903 0.0019054268818270396 64-65 4.521351922979416E-4 0.0 0.0 2.2468858381246206 0.0019054268818270396 66-67 4.521351922979416E-4 0.0 0.0 2.5136617492658457 0.0019215745672662519 68-69 4.521351922979416E-4 0.0 0.0 2.8077918395410957 0.001937722252705464 70-71 4.84430563176366E-4 0.0 0.0 3.1191515101799854 0.001953869938144676 72-73 5.167259340547904E-4 0.0 0.0 3.437809934637399 0.002115346792536798 74-75 5.167259340547904E-4 0.0 0.0 3.7597786346098507 0.002244528276050496 76-77 5.167259340547904E-4 0.0 0.0 4.115043861957959 0.002260675961489708 78-79 5.167259340547904E-4 0.0 0.0 4.5117440501430846 0.00227682364692892 80-81 5.167259340547904E-4 0.0 0.0 4.929000241892328 0.0023091190178073442 82-83 5.490213049332148E-4 0.0 0.0 5.365036191807375 0.00242215281588183 84-85 5.813166758116392E-4 0.0 0.0 5.810276322422773 0.0024544481867602545 86-87 5.813166758116392E-4 0.0 0.0 6.27214856904056 0.0025351866139563155 88-89 5.813166758116392E-4 0.0 0.0 6.759598749394058 0.0026159250411523765 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6535 0.0 34.425922 1 GTATCAA 10130 0.0 32.162605 1 TAAGGTG 2350 0.0 25.8954 5 ATCAACG 12625 0.0 25.41867 3 TATCAAC 12705 0.0 25.381954 2 TCAACGC 12705 0.0 25.183777 4 CAACGCA 12850 0.0 24.973598 5 AGGTAAG 2225 0.0 23.94187 2 AACGCAG 13415 0.0 23.863201 6 GTGTAAG 1280 0.0 23.781544 1 GTAAGGT 2625 0.0 23.001432 4 GTACATG 8990 0.0 22.48533 1 GGTAAGG 2565 0.0 22.056677 3 TACATGG 8995 0.0 21.838306 2 AAGGTAA 2380 0.0 21.783094 1 ACATGGG 8820 0.0 21.399439 3 ACGCAGA 15025 0.0 21.242926 7 CGCAGAG 15210 0.0 21.015772 8 AGAGTAC 13035 0.0 20.532526 10-11 GAGTACT 9305 0.0 19.498737 12-13 >>END_MODULE