FastQCFastQC Report
Thu 26 May 2016
SRR938473_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938473_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3108302
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72560.2334393504878226No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA58840.1892994953514813No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA58090.1868866023957775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55730.17929403256182957No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA53530.17221621322509847No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA48410.1557441973141606No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC42290.13605499079561767No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG42230.13586195935916137No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA41280.13280562828193657No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA40680.13087531391737353No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA38260.12308971264696932No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37090.11932559963607141No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG36110.11617275284061844No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT32050.10311095897374194No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA31110.10008679980259318No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68800.033.944231
GTATCAA109150.033.7094651
ATCAACG130600.027.766063
TATCAAC132250.027.527452
TCAACGC132850.027.3315774
CAACGCA133400.027.1476385
AACGCAG136500.026.5251186
GTACATG92100.024.1148511
TAAGGTG22700.023.4490415
ACGCAGA153500.023.3968587
CGCAGAG155350.023.1488158
TACATGG93200.022.794212
TAAGGTA13150.022.0463664
AGGTAAG21550.021.8333722
ACATGGG93150.021.4798953
GAGTACT89750.021.3811812-13
GGTAAGG23700.021.2564373
GTGTAAG14800.021.2539941
GCAGAGT167400.020.716279
GTAAGGT24600.020.6719574
Produced by FastQC (version 0.11.5)