FastQCFastQC Report
Thu 26 May 2016
SRR938473_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938473_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3108302
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71320.22945003413439233No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA58970.18971773013046994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57870.1861788204621044No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA57830.1860501328378002No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA56990.18334769272741194No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC50510.1625002975901312No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA49650.15973351366759087No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG45390.14602828167919335No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA39630.12749726377938822No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39200.12611387181811806No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA39190.12608169991204202No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA38060.1224462745254483No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG36770.11829609864163779No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA35490.11417809466390333No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT33780.10867669872489868No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71850.037.2684941
GTATCAA110900.031.5221631
TAAGGTG24250.029.7986225
AGGTAAG22600.027.569132
GTAAGGT24750.027.4678844
GGTAAGG24700.026.3686563
TCAACGC133850.025.785924
ATCAACG134850.025.5594463
TATCAAC136550.025.4267792
CAACGCA136100.025.3596275
AACGCAG140000.024.6629126
GCGTATC1000.001641762823.7810061
GTATCGC1401.7825125E-523.7469626
AAGGTAA24450.023.7323721
GTGTAAG13550.021.7626911
ACGCAGA158650.021.703817
CGCAGAG160200.021.4641698
GAGTACT97000.020.7847112-13
GTACATG94750.020.7315161
TACATGG91950.020.5352042
Produced by FastQC (version 0.11.5)