##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938473_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3108302 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.750262040174988 31.0 31.0 34.0 30.0 34.0 2 31.982937629612568 31.0 31.0 34.0 30.0 34.0 3 32.05416140387903 33.0 31.0 34.0 30.0 34.0 4 35.73583294029988 37.0 35.0 37.0 33.0 37.0 5 35.602160922587316 37.0 35.0 37.0 33.0 37.0 6 35.639232609958746 37.0 35.0 37.0 33.0 37.0 7 35.56860594626906 37.0 35.0 37.0 33.0 37.0 8 35.54270852703502 37.0 35.0 37.0 33.0 37.0 9 37.1110355428784 39.0 37.0 39.0 33.0 39.0 10-11 37.037731854884115 39.0 37.0 39.0 33.0 39.0 12-13 36.93484416893854 39.0 37.0 39.0 33.0 39.0 14-15 37.98469646771775 40.0 37.0 41.0 33.0 41.0 16-17 37.90669101650998 40.0 37.0 41.0 33.0 41.0 18-19 37.904770675436296 40.0 37.0 41.0 33.0 41.0 20-21 37.8825878244778 40.0 37.0 41.0 32.5 41.0 22-23 37.92123545266837 40.0 37.0 41.0 32.5 41.0 24-25 37.8500285686526 40.0 37.0 41.0 32.0 41.0 26-27 37.67117641722072 40.0 37.0 41.0 32.0 41.0 28-29 37.54312853126884 39.5 36.5 41.0 32.0 41.0 30-31 37.28915497914939 39.0 36.0 41.0 31.0 41.0 32-33 37.06852471220621 39.0 36.0 41.0 31.0 41.0 34-35 36.902771995771324 39.0 36.0 40.5 30.0 41.0 36-37 36.90743804816906 39.0 36.0 41.0 30.0 41.0 38-39 36.74843676708377 39.0 35.0 41.0 30.0 41.0 40-41 36.57216528509778 39.0 35.0 40.5 30.0 41.0 42-43 36.3409786114734 39.0 35.0 40.0 30.0 41.0 44-45 36.0713070995032 38.5 35.0 40.0 29.5 41.0 46-47 35.73830326010793 38.0 35.0 40.0 28.5 41.0 48-49 35.3493058267826 38.0 34.0 40.0 27.0 41.0 50-51 34.97342391440729 38.0 34.0 40.0 26.0 41.0 52-53 34.453649291478115 38.0 33.0 40.0 24.5 41.0 54-55 34.122938504688406 37.5 33.0 40.0 24.0 41.0 56-57 33.75430990939748 37.0 33.0 40.0 23.0 41.0 58-59 33.30600211948517 36.5 32.0 39.0 22.5 41.0 60-61 32.88883560863777 36.0 31.0 39.0 21.5 40.0 62-63 32.41695401540777 35.5 31.0 39.0 19.5 40.0 64-65 31.922840830781563 35.0 30.5 38.5 18.0 40.0 66-67 31.426157593438475 35.0 30.0 38.0 15.5 40.0 68-69 30.872894107458027 34.0 29.0 37.5 12.0 40.0 70-71 30.292286270767768 34.0 29.0 37.0 8.0 39.0 72-73 29.70646931990521 34.0 28.0 36.0 7.0 39.0 74-75 29.092561630111874 33.5 27.5 36.0 4.0 38.5 76-77 27.831483716833176 31.5 26.0 34.5 2.0 36.5 78-79 28.45215876706961 33.0 27.0 35.0 2.0 37.0 80-81 28.15499523534071 33.0 26.0 35.0 2.0 37.0 82-83 27.653605408998224 33.0 26.0 35.0 2.0 36.0 84-85 27.060635195679183 32.0 25.5 35.0 2.0 36.0 86-87 26.426095984238337 32.0 24.5 34.5 2.0 35.0 88-89 25.6576103287261 31.5 22.0 34.0 2.0 35.0 90-91 25.107680817372316 31.0 20.0 34.0 2.0 35.0 92-93 24.340375066515417 31.0 17.0 34.0 2.0 35.0 94-95 23.542592547313614 31.0 4.5 34.0 2.0 35.0 96-97 22.561387374843243 30.0 2.0 34.0 2.0 35.0 98-99 21.373164512328596 29.5 2.0 34.0 2.0 35.0 100-101 18.230956644495933 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34955438237262015 1101 2 0.3793207046941234 1101 3 0.32476867540829346 1101 4 0.21118406914616372 1101 5 0.17590119050463215 1101 6 0.23919317702084442 1101 7 0.17323252190259808 1101 8 0.28743965358665235 1101 9 0.4528982789820546 1101 10-11 0.40395954703452475 1101 12-13 0.42850679846560524 1101 14-15 0.6089143640018548 1101 16-17 0.6502950406622361 1101 18-19 0.6158626887687433 1101 20-21 0.6779032876283466 1101 22-23 0.5939366987039207 1101 24-25 0.5204958612295272 1101 26-27 0.5068455521850339 1101 28-29 0.5122317999256438 1101 30-31 0.6369283259291478 1101 32-33 0.5287132699495913 1101 34-35 0.28373319125437035 1101 36-37 0.5630976473875151 1101 38-39 0.7534339725238297 1101 40-41 0.7661861902055449 1101 42-43 0.6930284363993451 1101 44-45 0.7253622131505963 1101 46-47 0.9029576070358232 1101 48-49 0.8679769214570143 1101 50-51 0.958601105001712 1101 52-53 1.0710096424714521 1101 54-55 1.0068241988462248 1101 56-57 1.0931239760988802 1101 58-59 1.2576992336619028 1101 60-61 1.206914469478484 1101 62-63 1.1648477909562303 1101 64-65 1.2619682424053025 1101 66-67 1.35867892027321 1101 68-69 1.4544527986356606 1101 70-71 1.5566080345766444 1101 72-73 1.5948582692055062 1101 74-75 1.713285059560686 1101 76-77 1.6243773066477942 1101 78-79 1.904403455699292 1101 80-81 2.2015837830186697 1101 82-83 2.221867124718564 1101 84-85 2.411382756590463 1101 86-87 2.4606265504285254 1101 88-89 2.5462673431095872 1101 90-91 2.744799645815263 1101 92-93 3.0843716894843993 1101 94-95 3.135627872374382 1101 96-97 3.4584315935333834 1101 98-99 3.7760601804127525 1101 100-101 4.232893067328835 1102 1 0.31548503826458685 1102 2 0.32081106488303845 1102 3 0.31278109758181216 1102 4 0.20720296095120005 1102 5 0.19428312123267943 1102 6 0.1544656561343345 1102 7 0.27399763399377264 1102 8 0.26771196735175096 1102 9 0.32043137120736986 1102 10-11 0.3486815963925167 1102 12-13 0.4012841403249041 1102 14-15 0.4953907297043898 1102 16-17 0.42763257083831974 1102 18-19 0.5040087725673104 1102 20-21 0.5526832690491972 1102 22-23 0.46874614220137545 1102 24-25 0.4904790558205292 1102 26-27 0.5678208671737153 1102 28-29 0.4136209067737724 1102 30-31 0.2908646698056998 1102 32-33 0.3123526781827337 1102 34-35 0.4019225362041823 1102 36-37 0.6153839345946608 1102 38-39 0.6103702773180331 1102 40-41 0.583496723499195 1102 42-43 0.5743879609175053 1102 44-45 0.5642061573410047 1102 46-47 0.5905649557194934 1102 48-49 0.6067614003540527 1102 50-51 0.7043192731820724 1102 52-53 0.7575926729165943 1102 54-55 0.6081971917422564 1102 56-57 0.8509424596437114 1102 58-59 0.9138303819914384 1102 60-61 0.9640532809542606 1102 62-63 1.151871489889885 1102 64-65 0.9914117398544278 1102 66-67 1.0175375955019241 1102 68-69 1.1497860057029534 1102 70-71 1.196006046187108 1102 72-73 1.2870618822181257 1102 74-75 1.3916014521999749 1102 76-77 1.308119892275709 1102 78-79 1.588614187711645 1102 80-81 1.8143151880891537 1102 82-83 1.6546085108190738 1102 84-85 1.709801665230966 1102 86-87 1.945789087894969 1102 88-89 2.1937517327184928 1102 90-91 2.4132838821011156 1102 92-93 2.725538628224328 1102 94-95 2.788330837521947 1102 96-97 3.032761286195825 1102 98-99 3.376394544918771 1102 100-101 3.919040419800492 1103 1 0.31087786474911283 1103 2 0.3167562744527075 1103 3 0.3691883681375572 1103 4 0.2763896888278836 1103 5 0.14232178804864049 1103 6 0.22071430316901086 1103 7 0.2726241409172232 1103 8 0.33571928269531526 1103 9 0.4720679927129865 1103 10-11 0.48358548920034394 1103 12-13 0.5251709764388153 1103 14-15 0.6457635882868544 1103 16-17 0.723598681389582 1103 18-19 0.630244729720296 1103 20-21 0.560359901005306 1103 22-23 0.4963944085395937 1103 24-25 0.46239560118685574 1103 26-27 0.5288241142905008 1103 28-29 0.5552668590747487 1103 30-31 0.5781785204383283 1103 32-33 0.6896236071567259 1103 34-35 0.7094536077527209 1103 36-37 0.697303839047315 1103 38-39 0.7431198760938358 1103 40-41 0.6428047517155164 1103 42-43 0.8524083393745698 1103 44-45 0.9600184963847838 1103 46-47 0.9817843040567524 1103 48-49 1.1111894225986703 1103 50-51 1.0596941845395165 1103 52-53 1.1085462802020842 1103 54-55 1.0702992333202843 1103 56-57 0.9752935961345841 1103 58-59 1.1988234772028719 1103 60-61 1.1242996139516848 1103 62-63 1.2324880688470756 1103 64-65 1.2317707888597624 1103 66-67 1.306812406086781 1103 68-69 1.4759667245474297 1103 70-71 1.542409957656517 1103 72-73 1.5231631026861585 1103 74-75 1.559119833866827 1103 76-77 1.4779985919360676 1103 78-79 1.6371119296591665 1103 80-81 1.9043991652473657 1103 82-83 1.9940964367698832 1103 84-85 2.2584472069769426 1103 86-87 2.310145449631243 1103 88-89 2.4938062245633787 1103 90-91 2.6348813142662344 1103 92-93 2.830864646214099 1103 94-95 2.923525851144909 1103 96-97 3.311506480836215 1103 98-99 3.7210942799241806 1103 100-101 4.05812003014648 1104 1 0.28006218110573045 1104 2 0.29573420339609413 1104 3 0.301986726905362 1104 4 0.24660945757354824 1104 5 0.19309779420392204 1104 6 0.23607796964969907 1104 7 0.19390379327104768 1104 8 0.24225526370310746 1104 9 0.3356373756698474 1104 10-11 0.30390809351879255 1104 12-13 0.3719549951097463 1104 14-15 0.5413762672588831 1104 16-17 0.5048306749869624 1104 18-19 0.5331161378882356 1104 20-21 0.6078826105963913 1104 22-23 0.5132391867337205 1104 24-25 0.5323082220322704 1104 26-27 0.503546467046057 1104 28-29 0.6390173642305044 1104 30-31 0.6713143326005948 1104 32-33 0.6239714586452862 1104 34-35 0.6182454468343366 1104 36-37 0.7053841305627557 1104 38-39 0.7601411906133464 1104 40-41 0.7627509249151956 1104 42-43 0.8983526194710905 1104 44-45 0.8770678234617648 1104 46-47 0.876868969426063 1104 48-49 0.9873411859649579 1104 50-51 1.0241039882304577 1104 52-53 1.2383365678105136 1104 54-55 1.2899777578660903 1104 56-57 1.395657284070289 1104 58-59 1.4553678916848583 1104 60-61 1.5221602291430898 1104 62-63 1.702864404243428 1104 64-65 1.642248607612352 1104 66-67 1.7612694999478755 1104 68-69 1.7956143208532431 1104 70-71 1.6629697444970226 1104 72-73 1.892203021240693 1104 74-75 1.979156470243037 1104 76-77 1.799007151829855 1104 78-79 2.0938949680006154 1104 80-81 2.3014466350895795 1104 82-83 2.42765589870433 1104 84-85 2.6274909214200584 1104 86-87 2.609424967973691 1104 88-89 2.5876112683860875 1104 90-91 2.7603638734785214 1104 92-93 2.9289899063562466 1104 94-95 3.201196311022862 1104 96-97 3.720820502155469 1104 98-99 3.899750526564958 1104 100-101 4.13129772031197 1105 1 0.24723102313916812 1105 2 0.2667096491309522 1105 3 0.24568757263799768 1105 4 0.20735519941437985 1105 5 0.14721223519826054 1105 6 0.17606284759717283 1105 7 0.21692133430710925 1105 8 0.15688597525896597 1105 9 0.3238632986316219 1105 10-11 0.34346103107598225 1105 12-13 0.36789502996843026 1105 14-15 0.5659660532931525 1105 16-17 0.49955917483786294 1105 18-19 0.49975593860238376 1105 20-21 0.46104194811804433 1105 22-23 0.38704051364353376 1105 24-25 0.3855803567375702 1105 26-27 0.45854039077232045 1105 28-29 0.6181934743213873 1105 30-31 0.6683315727044601 1105 32-33 0.6712085930079468 1105 34-35 0.6561766825696509 1105 36-37 0.690812023247318 1105 38-39 0.6586582508075409 1105 40-41 0.6847477693183777 1105 42-43 0.7430724434215676 1105 44-45 0.7820564452488767 1105 46-47 0.7996313467820428 1105 48-49 0.947154792417308 1105 50-51 0.9601707750604405 1105 52-53 1.132470045490713 1105 54-55 1.0595957185726448 1105 56-57 1.102624136891606 1105 58-59 1.376868753302361 1105 60-61 1.2848781246965686 1105 62-63 1.498370497386972 1105 64-65 1.5249322543154378 1105 66-67 1.4333915581313228 1105 68-69 1.5829461021643958 1105 70-71 1.4930564259630117 1105 72-73 1.5424081098868108 1105 74-75 1.6489538481512191 1105 76-77 1.6242310854118536 1105 78-79 1.9722141024056086 1105 80-81 2.3313539203102245 1105 82-83 2.3779479140049276 1105 84-85 2.386572011540661 1105 86-87 2.5831080176323233 1105 88-89 2.627961749615654 1105 90-91 2.7977350525983518 1105 92-93 3.135516153365 1105 94-95 3.12555576657029 1105 96-97 3.438060595545039 1105 98-99 3.7397606882657186 1105 100-101 4.296083089223199 1106 1 0.19684394079995826 1106 2 0.17593998824725787 1106 3 0.18317684562462233 1106 4 0.1867274887110213 1106 5 0.042218192697163204 1106 6 0.12965060190752098 1106 7 0.089676726143459 1106 8 0.06562333702783008 1106 9 0.26180894787906794 1106 10-11 0.21979629868368988 1106 12-13 0.15290376253276605 1106 14-15 0.057937594604197784 1106 16-17 0.1277525162523787 1106 18-19 0.3032539065183286 1106 20-21 0.40215752028814933 1106 22-23 0.18808321036197384 1106 24-25 0.19103999814617367 1106 26-27 0.20904474133876505 1106 28-29 0.32020138511896334 1106 30-31 0.37872289001686 1106 32-33 0.443514784050727 1106 34-35 0.35164457886740763 1106 36-37 0.43732819948009904 1106 38-39 0.45834556999921006 1106 40-41 0.5380280745011277 1106 42-43 0.6411839100663954 1106 44-45 0.24417814613652666 1106 46-47 0.12644730866066567 1106 48-49 0.5920045232442277 1106 50-51 0.34316578614622983 1106 52-53 0.6164126111482986 1106 54-55 0.7885713927046964 1106 56-57 0.7254587276893929 1106 58-59 1.0038447467116853 1106 60-61 0.8112327681337419 1106 62-63 1.0402798788865084 1106 64-65 0.6788418914022287 1106 66-67 0.7361643282463071 1106 68-69 0.6628370722834305 1106 70-71 0.8762078705065761 1106 72-73 1.3118561076005868 1106 74-75 1.5862628039201496 1106 76-77 1.5855500438235097 1106 78-79 1.8407078456876569 1106 80-81 1.7428736884123595 1106 82-83 2.090534676157006 1106 84-85 2.101416978022751 1106 86-87 2.3725792911557555 1106 88-89 2.6908759157250266 1106 90-91 2.659610823133942 1106 92-93 2.3095901975700137 1106 94-95 2.742854287927752 1106 96-97 3.280435637987825 1106 98-99 3.678085272491849 1106 100-101 4.0797967223215 1107 1 0.13912201326478169 1107 2 0.1337396469241554 1107 3 0.16430274661490074 1107 4 0.09717285483691995 1107 5 0.0150195871658525 1107 6 0.01862843246545509 1107 7 0.07785747243732999 1107 8 0.09613088806710834 1107 9 0.09636837514071317 1107 10-11 0.18374825993127786 1107 12-13 0.17014034257543642 1107 14-15 0.33317335573953244 1107 16-17 0.23067555611368817 1107 18-19 0.300104987016681 1107 20-21 0.2393025479100075 1107 22-23 0.2434449637620446 1107 24-25 0.207876636383709 1107 26-27 0.258996413189152 1107 28-29 0.3460505874925701 1107 30-31 0.3455248617028559 1107 32-33 0.25024994204523665 1107 34-35 0.25974785818321067 1107 36-37 0.3535777241333733 1107 38-39 0.4733399131513565 1107 40-41 0.42162889325075525 1107 42-43 0.48797559316935946 1107 44-45 0.5779298535898576 1107 46-47 0.6226023967779781 1107 48-49 0.6912530762089517 1107 50-51 0.6232831536647083 1107 52-53 0.6700558222507453 1107 54-55 0.6526932466683277 1107 56-57 0.6843546162580552 1107 58-59 0.9161132856284127 1107 60-61 0.8929063552968088 1107 62-63 0.9734424816713059 1107 64-65 0.9579429681056837 1107 66-67 1.1120409228140353 1107 68-69 1.2380291379900008 1107 70-71 1.3084965647160516 1107 72-73 1.4250387513386364 1107 74-75 1.3608497377154158 1107 76-77 1.4111650670866638 1107 78-79 1.6340650365476712 1107 80-81 1.8366761330301529 1107 82-83 1.8719652721831537 1107 84-85 2.1979165532909626 1107 86-87 2.304926880061121 1107 88-89 2.415411869877257 1107 90-91 2.570374614891758 1107 92-93 2.7916039545824276 1107 94-95 3.010128915422495 1107 96-97 3.1329258141554135 1107 98-99 3.3237376216854493 1107 100-101 3.5336752756430343 1108 1 0.16783289684195069 1108 2 0.16870326982442663 1108 3 0.10319910701621637 1108 4 0.10218404544048099 1108 5 0.03798962723426769 1108 6 0.04685741737855409 1108 7 0.09280105279719209 1108 8 0.08984731833956516 1108 9 0.13366288771757695 1108 10-11 0.059762816326191626 1108 12-13 0.15689340658991568 1108 14-15 0.2351100328663165 1108 16-17 0.25455051021527453 1108 18-19 0.2764729265068766 1108 20-21 0.25160485141339706 1108 22-23 0.19905112562111782 1108 24-25 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2316 62-63 0.32326951979286633 2316 64-65 0.37832401434400253 2316 66-67 0.4873807421148122 2316 68-69 0.6074231209143726 2316 70-71 0.6937847476884116 2316 72-73 0.8625045382311818 2316 74-75 1.070486877125628 2316 76-77 0.9606608760925859 2316 78-79 1.0881371871445786 2316 80-81 1.173967406180278 2316 82-83 1.3698196714520314 2316 84-85 1.486099022098724 2316 86-87 1.6657835968717656 2316 88-89 1.9360470830534702 2316 90-91 2.0865940197102653 2316 92-93 1.916677553677271 2316 94-95 2.4487185448144864 2316 96-97 2.6622310945488508 2316 98-99 3.0343078021475485 2316 100-101 3.4928704084848796 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 25.0 4 37.0 5 96.0 6 197.0 7 605.0 8 1371.0 9 2527.0 10 4085.0 11 6535.0 12 9253.0 13 12643.0 14 16723.0 15 21506.0 16 26793.0 17 32408.0 18 31719.0 19 28949.0 20 29374.0 21 31251.0 22 34308.0 23 39199.0 24 44832.0 25 52168.0 26 61457.0 27 73609.0 28 88092.0 29 103811.0 30 122435.0 31 144940.0 32 172806.0 33 208270.0 34 258815.0 35 325563.0 36 408429.0 37 449162.0 38 248886.0 39 15417.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.91703688547137 19.264246627654952 15.05303386388109 23.76568262299259 2 15.757223074442262 20.477238214848285 39.242844516656056 24.5226941940534 3 19.324311672919286 23.48916464546342 30.295013636084324 26.891510045532975 4 13.325410465263671 16.924771145146128 36.46778852247948 33.28202986711072 5 13.576747033463962 37.397241613398855 34.65292808466773 14.373083268469458 6 32.90468558074473 36.376066418256656 16.72314980976752 13.996098191231097 7 28.759367654751696 32.60715979335341 20.350467876030066 18.283004675864827 8 26.5193665223006 35.200537142143844 19.52207346647784 18.758022869077713 9 26.07584462513617 16.71539638040319 19.936898023422433 37.27186097103821 10-11 24.970128385208387 26.426405799693853 27.491955414885684 21.111510400212076 12-13 26.009490068854312 23.603755362252446 27.560915895559695 22.825838673333543 14-15 22.207687669988307 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28.532075214898445 22.782557410153377 40-41 22.655173423395162 26.475208062065185 28.556440022431808 22.313178492107845 42-43 22.27305463856479 26.549465943255196 28.339151651138373 22.838327767041644 44-45 22.281886557652577 26.75019673626916 28.677359596579656 22.290557109498614 46-47 22.880538274768114 27.086919831766615 27.648995383651716 22.38354650981356 48-49 22.36595736192944 27.11115264861651 27.71812391460032 22.80476607485373 50-51 22.61141291933667 27.27667710537779 27.763373700496285 22.348536274789257 52-53 23.10552513880569 26.816088012040012 27.7482368186875 22.330150030466793 54-55 22.285808227710252 27.152791830649605 28.05011483765568 22.51128510398446 56-57 22.452403273555788 27.298377056026084 27.66761402206092 22.581605648357204 58-59 22.468489226593814 26.859262709994074 27.587602491649783 23.084645571762334 60-61 21.805892735004516 27.233180688362975 28.189104533600663 22.771822043031857 62-63 22.11676986341739 27.17353397449797 28.21719060760505 22.49250555447958 64-65 21.71582748394461 27.28525091834706 27.913326954716755 23.085594642991573 66-67 21.657464677988305 27.32706719808332 27.85677873934444 23.15868938458393 68-69 22.550495890435197 26.805220542756924 27.91008582672669 22.734197740081186 70-71 22.22518918689368 26.965060023125165 27.45314001020493 23.356610779776226 72-73 22.13310422639249 26.995953738071055 27.66186415022226 23.209077885314198 74-75 22.19317814034801 27.478990136737035 27.316940245831965 23.010891477082986 76-77 22.223821880885446 26.89370273544849 27.774392578327333 23.108082805338732 78-79 22.333479822745666 27.176123812937096 27.60236939653869 22.88802696777855 80-81 22.68485624218122 27.164989356724035 27.212989863753524 22.937164537341225 82-83 22.41285872228343 27.305594122006394 27.465860044722607 22.81568711098757 84-85 22.287387576224724 27.451807121348104 27.430524443469235 22.83028085895794 86-87 22.628964942576346 27.336666926726632 27.027329621178424 23.007038509518594 88-89 22.27959371354015 27.566712924542824 26.707207590516933 23.446485771400084 90-91 22.137675342771168 27.536435874758585 27.0127521137971 23.313136668673142 92-93 22.4238119045367 27.031275033828845 27.300506322760974 23.24440673887348 94-95 22.00703149179198 26.825626982191565 27.508298743172315 23.65904278284414 96-97 22.30991390154496 26.31969158723959 27.625870973927242 23.744523537288202 98-99 22.015701820479478 26.778446881931036 27.079431149225524 24.126420148363962 100-101 22.021736144020533 26.3186477147 27.63062775252801 24.02898838875146 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 186.0 1 142.0 2 143.5 3 213.0 4 192.0 5 171.5 6 334.0 7 755.0 8 1235.5 9 1423.0 10 1281.5 11 1135.5 12 1186.5 13 1380.5 14 1611.0 15 1796.5 16 1980.0 17 2291.0 18 2683.5 19 3081.5 20 3727.5 21 4556.5 22 5581.5 23 7013.5 24 9055.5 25 11877.0 26 15654.0 27 20443.5 28 25173.0 29 30026.0 30 35916.5 31 45139.5 32 58665.5 33 62893.5 34 61262.5 35 65589.0 36 71874.0 37 78159.0 38 83072.5 39 87097.0 40 92876.0 41 100025.0 42 103397.0 43 105809.5 44 108619.0 45 114176.5 46 117544.0 47 119903.5 48 122635.0 49 118624.0 50 113748.5 51 110020.0 52 107008.5 53 107034.0 54 108349.5 55 101592.5 56 91817.0 57 83650.0 58 76322.0 59 66303.5 60 51756.5 61 42995.5 62 37637.5 63 27590.0 64 18119.5 65 13419.0 66 10188.0 67 8074.5 68 6334.5 69 4758.5 70 3567.0 71 2454.0 72 1699.5 73 1275.0 74 945.5 75 697.0 76 509.0 77 360.5 78 235.5 79 130.5 80 72.5 81 44.5 82 29.0 83 17.0 84 8.5 85 8.0 86 6.0 87 3.5 88 2.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.6085953038025261E-4 2 0.04452591800925393 3 1.6085953038025261E-4 4 0.0 5 0.09912164262031167 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0012547043369659705 26-27 0.011855347389024619 28-29 0.0 30-31 0.0 32-33 4.986645441787831E-4 34-35 0.01500819418447757 36-37 0.003233276560643078 38-39 0.012241410261937225 40-41 0.006916959806350863 42-43 0.005099247113054008 44-45 0.002573752486084042 46-47 3.217190607605053E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 3.217190607605053E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.0911738949432843E-4 68-69 1.4477357734222736E-4 70-71 0.0 72-73 3.217190607605053E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.8257859114075794E-5 82-83 9.812431353195409E-4 84-85 0.004520152803685099 86-87 0.0014316498203842485 88-89 8.042976519012631E-5 90-91 0.0010616729005096673 92-93 2.573752486084042E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.39953839749162E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3108302.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.889381920919256 #Duplication Level Percentage of deduplicated Percentage of total 1 72.92285213316079 36.3807602083398 2 14.60252061330328 14.570214557703668 3 5.243324505289592 7.847586563391225 4 2.4782976112260653 4.9456294416063615 5 1.3614694389030444 3.3961434405546815 6 0.7774734882559884 2.3272603073395404 7 0.5300955840679165 1.8512298733709933 8 0.37605471651090444 1.5008909900140426 9 0.2658114431477958 1.1935051745535992 >10 1.300249069988869 11.47339681051019 >50 0.08420915540050615 2.91357113058426 >100 0.048609716167081915 4.651367421440672 >500 0.004516262289427862 1.4903846534674086 >1k 0.0041291540924869 4.320258267193218 >5k 3.87108196170647E-4 1.1378011599303615 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7132 0.22945003413439233 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 5897 0.18971773013046994 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5787 0.1861788204621044 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5783 0.1860501328378002 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5699 0.18334769272741194 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5051 0.1625002975901312 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4965 0.15973351366759087 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4539 0.14602828167919335 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3963 0.12749726377938822 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3920 0.12611387181811806 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3919 0.12608169991204202 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3806 0.1224462745254483 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3677 0.11829609864163779 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3549 0.11417809466390333 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3378 0.10867669872489868 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.434381215210105E-5 2 3.217190607605053E-5 0.0 0.0 0.0 6.434381215210105E-5 3 3.217190607605053E-5 0.0 0.0 0.0 6.434381215210105E-5 4 3.217190607605053E-5 0.0 0.0 0.0 6.434381215210105E-5 5 3.217190607605053E-5 0.0 0.0 0.0 6.434381215210105E-5 6 3.217190607605053E-5 0.0 0.0 0.0 6.434381215210105E-5 7 3.217190607605053E-5 0.0 0.0 0.0 6.434381215210105E-5 8 3.217190607605053E-5 0.0 0.0 0.0 6.434381215210105E-5 9 3.217190607605053E-5 0.0 0.0 0.0 6.434381215210105E-5 10-11 3.217190607605053E-5 0.0 0.0 0.0 1.1260167126617684E-4 12-13 3.217190607605053E-5 0.0 0.0 0.0 3.2171906076050523E-4 14-15 3.217190607605053E-5 0.0 0.0 0.0 4.8257859114075787E-4 16-17 3.217190607605053E-5 0.0 0.0 0.0 6.75610027597061E-4 18-19 8.042976519012632E-5 0.0 0.0 0.0 7.077819336731116E-4 20-21 1.286876243042021E-4 0.0 0.0 3.217190607605053E-5 8.364695579773136E-4 22-23 1.286876243042021E-4 0.0 0.0 1.769454834182779E-4 8.364695579773136E-4 24-25 1.6085953038025261E-4 0.0 0.0 5.147504972168084E-4 8.364695579773136E-4 26-27 1.6085953038025261E-4 0.0 0.0 0.0010295009944336169 9.008133701294147E-4 28-29 1.7694548341827787E-4 0.0 0.0 0.003506737762289507 9.329852762054652E-4 30-31 1.9303143645630315E-4 0.0 0.0 0.012386183839279453 9.651571822815157E-4 32-33 2.0911738949432843E-4 0.0 0.0 0.03326575088263624 0.001109930759623743 34-35 2.2520334253235368E-4 0.0 0.0 0.06415078071564476 0.0011903605248138693 36-37 2.2520334253235368E-4 0.0 0.0 0.11512716589314681 0.0011903605248138693 38-39 2.2520334253235368E-4 0.0 0.0 0.1920501933209836 0.0011903605248138693 40-41 2.2520334253235368E-4 0.0 0.0 0.2739759521436463 0.0011903605248138693 42-43 2.895471546844547E-4 0.0 0.0 0.3613387630931615 0.00122253243088992 44-45 3.2171906076050523E-4 0.0 0.0 0.47275007383452444 0.0012547043369659705 46-47 3.538909668365558E-4 0.0 0.0 0.6021293941193617 0.0013190481491180714 48-49 3.538909668365558E-4 0.0 0.0 0.7423023888927137 0.001351220055194122 50-51 3.538909668365558E-4 0.0 0.0 0.8942985591490145 0.0013833919612701725 52-53 3.6997691987458107E-4 0.0 0.0 1.0392973398337744 0.001479907679498324 54-55 3.860628729126063E-4 0.0 0.0 1.1994008304212396 0.0015442514916504252 56-57 4.5040668506470737E-4 0.0 0.0 1.3860944013805607 0.0015442514916504252 58-59 4.5040668506470737E-4 0.0 0.0 1.584225084949918 0.001592509350764501 60-61 4.5040668506470737E-4 0.0 0.0 1.7972352750794487 0.0016246812568405516 62-63 4.5040668506470737E-4 0.0 0.0 2.01463693038836 0.0016729391159546272 64-65 4.5040668506470737E-4 0.0 0.0 2.2386016545367857 0.0017051110220306779 66-67 4.8257859114075787E-4 0.0 0.0 2.504679403738762 0.0017211969750687032 68-69 4.8257859114075787E-4 0.0 0.0 2.79533648918284 0.0017694548341827788 70-71 4.986645441787832E-4 0.0 0.0 3.100117041394305 0.00183379864633488 72-73 5.147504972168084E-4 0.0 0.0 3.418441966063787 0.002042916035829208 74-75 5.147504972168084E-4 0.0 0.0 3.742220028813159 0.0022359474722855113 76-77 5.147504972168084E-4 1.6085953038025264E-5 0.0 4.098507802652381 0.002284205331399587 78-79 5.147504972168084E-4 3.217190607605053E-5 0.0 4.494881771462361 0.002316377237475638 80-81 5.147504972168084E-4 3.217190607605053E-5 0.0 4.909384609346196 0.0023646350965897136 82-83 5.790943093689094E-4 3.217190607605053E-5 0.0 5.340665096248692 0.0026541822512741683 84-85 5.790943093689094E-4 3.217190607605053E-5 0.0 5.785168236548444 0.0026702682043121934 86-87 5.790943093689094E-4 3.217190607605053E-5 0.0 6.245242579389004 0.0027828698755783705 88-89 5.790943093689094E-4 3.217190607605053E-5 3.217190607605053E-5 6.733145620985348 0.0028632996407684966 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7185 0.0 37.268494 1 GTATCAA 11090 0.0 31.522163 1 TAAGGTG 2425 0.0 29.798622 5 AGGTAAG 2260 0.0 27.56913 2 GTAAGGT 2475 0.0 27.467884 4 GGTAAGG 2470 0.0 26.368656 3 TCAACGC 13385 0.0 25.78592 4 ATCAACG 13485 0.0 25.559446 3 TATCAAC 13655 0.0 25.426779 2 CAACGCA 13610 0.0 25.359627 5 AACGCAG 14000 0.0 24.662912 6 GCGTATC 100 0.0016417628 23.781006 1 GTATCGC 140 1.7825125E-5 23.746962 6 AAGGTAA 2445 0.0 23.732372 1 GTGTAAG 1355 0.0 21.762691 1 ACGCAGA 15865 0.0 21.70381 7 CGCAGAG 16020 0.0 21.464169 8 GAGTACT 9700 0.0 20.78471 12-13 GTACATG 9475 0.0 20.731516 1 TACATGG 9195 0.0 20.535204 2 >>END_MODULE