FastQCFastQC Report
Thu 26 May 2016
SRR938472_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938472_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3117452
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67790.21745322782836754No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA56900.18252085356887612No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA56650.1817189166024048No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA53780.17251268022731386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51550.16535940248638953No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA49280.15807781483082978No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG43300.13889548259283543No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC42190.1353348824617027No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA41900.13440463558059595No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA39930.12808537228480182No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA37100.11900744582434629No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG36030.11557515560784896No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33370.10704254628459396No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT32610.10460465790652108No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70000.033.7861751
GTATCAA109150.033.200551
ATCAACG129050.027.7648453
TCAACGC129700.027.6257024
TATCAAC130200.027.5561082
CAACGCA130300.027.3890825
AACGCAG135800.026.3792786
GTACATG97500.025.6260741
TACATGG97850.025.5450572
ACATGGG99500.023.7364233
ACGCAGA151000.023.6889957
TAAGGTG22750.023.3947585
CGCAGAG153350.023.2020638
AGGTAAG21250.022.3626352
GTGTAAG15500.021.2262271
GAGTACT88400.021.01063312-13
GCAGAGT162850.020.8859339
AGAGTAC126850.020.48384710-11
GTAAGGT24950.020.3795784
GGTAAGG24350.020.1011183
Produced by FastQC (version 0.11.5)