##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938472_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3117452 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.849071613612654 31.0 31.0 34.0 27.0 34.0 2 31.034140060536618 31.0 31.0 34.0 27.0 34.0 3 31.080866682149395 31.0 31.0 34.0 27.0 34.0 4 34.75489021162154 37.0 35.0 37.0 32.0 37.0 5 34.742064352554586 37.0 35.0 37.0 32.0 37.0 6 34.705725060081114 37.0 35.0 37.0 31.0 37.0 7 34.61024002935731 37.0 35.0 37.0 30.0 37.0 8 34.55279118972802 37.0 35.0 37.0 30.0 37.0 9 36.00898650564628 39.0 35.0 39.0 30.0 39.0 10-11 35.83459857601657 38.0 35.0 39.0 30.0 39.0 12-13 35.647202908015906 38.0 35.0 39.0 30.0 39.0 14-15 36.7604761837552 39.0 36.0 41.0 30.0 41.0 16-17 36.681210648953055 39.0 36.0 41.0 30.0 41.0 18-19 36.670902390798645 39.0 36.0 41.0 30.0 41.0 20-21 36.58992744715877 39.0 36.0 41.0 30.0 41.0 22-23 36.43328237291224 39.0 36.0 41.0 29.0 41.0 24-25 36.28053904278237 39.0 35.5 41.0 29.0 41.0 26-27 36.05265133192107 39.0 35.0 40.0 27.5 41.0 28-29 35.816039028026736 38.5 35.0 40.0 27.0 41.0 30-31 35.52165919475264 38.0 34.0 40.0 27.0 41.0 32-33 35.20577862947048 38.0 34.0 40.0 25.5 41.0 34-35 34.88838144099732 38.0 34.0 40.0 25.0 41.0 36-37 34.632942383715935 38.0 33.5 40.0 24.5 41.0 38-39 34.36979847644807 38.0 33.0 40.0 24.0 41.0 40-41 34.083575785609526 38.0 33.0 40.0 23.5 41.0 42-43 33.656087728054835 38.0 32.5 40.0 21.5 41.0 44-45 33.18361020474413 37.0 31.5 40.0 18.5 41.0 46-47 32.69680014319387 37.0 31.0 40.0 15.5 41.0 48-49 32.20024414169008 37.0 30.5 39.5 11.0 41.0 50-51 31.47498325555614 35.5 30.0 38.5 9.0 40.0 52-53 31.481718403362745 36.0 30.0 39.0 8.5 40.0 54-55 31.45455038281263 36.0 30.0 39.0 7.0 40.0 56-57 31.017411976190814 36.0 29.0 39.0 2.0 40.0 58-59 30.55230232895326 35.0 28.0 39.0 2.0 40.0 60-61 29.989460463224454 35.0 28.0 38.5 2.0 40.0 62-63 29.256628650577458 34.0 26.5 38.0 2.0 40.0 64-65 28.59411051076328 34.0 26.0 38.0 2.0 40.0 66-67 27.885345788804447 33.5 24.5 37.0 2.0 39.5 68-69 27.034563964417096 33.0 22.5 36.5 2.0 39.0 70-71 26.17404470060806 32.0 19.5 36.0 2.0 39.0 72-73 25.27280965352474 31.0 15.5 35.0 2.0 38.0 74-75 24.343209294000356 31.0 7.5 35.0 2.0 37.5 76-77 23.39741157201458 30.0 4.0 34.5 2.0 36.5 78-79 22.409378556590447 29.0 2.0 34.0 2.0 36.0 80-81 21.449705881598177 28.5 2.0 34.0 2.0 35.5 82-83 20.44318886064645 26.5 2.0 33.5 2.0 35.0 84-85 19.471556578898408 26.0 2.0 33.0 2.0 35.0 86-87 18.496297136251016 24.5 2.0 32.0 2.0 34.5 88-89 17.389071106788492 21.0 2.0 32.0 2.0 34.0 90-91 16.246649988516264 14.5 2.0 31.0 2.0 34.0 92-93 15.10205979113712 4.5 2.0 31.0 2.0 34.0 94-95 13.864585084229043 2.0 2.0 30.0 2.0 34.0 96-97 12.473471925149127 2.0 2.0 29.0 2.0 34.0 98-99 10.824894497172691 2.0 2.0 27.0 2.0 33.5 100-101 7.700047185971107 2.0 2.0 2.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7119365885984266 1101 2 0.6068707433875637 1101 3 0.6550431859262282 1101 4 0.5414021716939104 1101 5 0.4744858549636106 1101 6 0.6676609398804061 1101 7 0.5877744093577135 1101 8 0.6906272586524906 1101 9 0.6658003529232701 1101 10-11 0.6751387091404553 1101 12-13 0.7231635648419328 1101 14-15 0.9185159448265026 1101 16-17 0.98716079099421 1101 18-19 0.8341936267627048 1101 20-21 1.0196961282752497 1101 22-23 0.898677689506961 1101 24-25 1.0545579356415544 1101 26-27 1.0474391564302223 1101 28-29 1.1755246762724667 1101 30-31 1.1367931617450964 1101 32-33 1.2086442447228336 1101 34-35 1.2644809779107362 1101 36-37 1.318010532311547 1101 38-39 1.407078804259541 1101 40-41 1.347418719178819 1101 42-43 1.4706550682856445 1101 44-45 1.4399795946809562 1101 46-47 1.5424001371972835 1101 48-49 1.3914841904359108 1101 50-51 1.5439296438504897 1101 52-53 1.4759414242275533 1101 54-55 1.7606171172786915 1101 56-57 1.8868746643768795 1101 58-59 1.7399959595989358 1101 60-61 1.7563294473541156 1101 62-63 1.5682394511869582 1101 64-65 1.8237313610700774 1101 66-67 2.167154840178007 1101 68-69 2.175637467816223 1101 70-71 2.636299642720008 1101 72-73 2.7533366643214165 1101 74-75 2.9424629532993016 1101 76-77 3.2115160291098306 1101 78-79 3.1570557369601744 1101 80-81 3.4565941574699224 1101 82-83 3.593804862838052 1101 84-85 3.660888567108433 1101 86-87 3.847025786784865 1101 88-89 3.983200552948791 1101 90-91 4.014090500617492 1101 92-93 3.9876590949555126 1101 94-95 3.854748006706764 1101 96-97 3.6606098209494817 1101 98-99 3.345932777032388 1101 100-101 2.4223726240691006 1102 1 0.6693146064391868 1102 2 0.6643621411394349 1102 3 0.6303193349030103 1102 4 0.4912514295516246 1102 5 0.5831118982962735 1102 6 0.7046717685789261 1102 7 0.6454358064371064 1102 8 0.720761344152514 1102 9 0.7802818502717059 1102 10-11 0.8591622757968835 1102 12-13 0.7972696340765637 1102 14-15 0.9356436709879148 1102 16-17 0.984711848399435 1102 18-19 1.0220020885438927 1102 20-21 0.9082127207269082 1102 22-23 0.9026540983969582 1102 24-25 0.9267217331678879 1102 26-27 0.8496561075498903 1102 28-29 0.9002468578693694 1102 30-31 0.8325386875650054 1102 32-33 0.9489759150567494 1102 34-35 1.0190950018441924 1102 36-37 1.1357780719862873 1102 38-39 1.2085180025775202 1102 40-41 1.206950485069754 1102 42-43 1.4167549945017086 1102 44-45 1.2048377240111776 1102 46-47 1.3061007192392395 1102 48-49 1.2693804099612365 1102 50-51 1.3846620235260048 1102 52-53 1.2372635440321709 1102 54-55 1.2875260945128844 1102 56-57 1.5528527906598093 1102 58-59 1.7352533193001136 1102 60-61 1.6777304463764402 1102 62-63 1.8262079639256221 1102 64-65 2.002508814315565 1102 66-67 2.2893790662343214 1102 68-69 2.1946910888489235 1102 70-71 1.9876897810660736 1102 72-73 2.3475330448170624 1102 74-75 2.1402294254817242 1102 76-77 2.3344665143956007 1102 78-79 2.667703385020655 1102 80-81 2.9483502481719697 1102 82-83 3.081437352977858 1102 84-85 3.1859889795742973 1102 86-87 3.2906476142282592 1102 88-89 3.4364687841422317 1102 90-91 3.1761500013226467 1102 92-93 3.2599768559115336 1102 94-95 3.3692172302448284 1102 96-97 3.3046626778081674 1102 98-99 2.925185910968816 1102 100-101 2.104369601231819 1103 1 0.6114977949045723 1103 2 0.60905231525172 1103 3 0.47550471095774327 1103 4 0.5107830183509066 1103 5 0.4797821459339602 1103 6 0.41145643902480344 1103 7 0.6114876734599477 1103 8 0.4622937855930829 1103 9 0.5566996188020781 1103 10-11 0.5926186027118518 1103 12-13 0.7424837603877066 1103 14-15 0.7957353551655473 1103 16-17 0.8535575038066199 1103 18-19 0.9746932318587653 1103 20-21 0.9328152421220963 1103 22-23 0.9081408542345386 1103 24-25 0.9324825686645681 1103 26-27 0.9349854000916693 1103 28-29 1.01665877997889 1103 30-31 1.0631023210834414 1103 32-33 1.2037475857059263 1103 34-35 1.1057955531240538 1103 36-37 1.211043398075688 1103 38-39 1.1829793196008325 1103 40-41 1.2668082665228297 1103 42-43 1.3369421143868365 1103 44-45 1.4891700733040807 1103 46-47 1.38526843947011 1103 48-49 1.262382888953816 1103 50-51 1.4570618462059883 1103 52-53 1.5949981890318945 1103 54-55 1.6006281462211263 1103 56-57 1.851261178300458 1103 58-59 1.9612425529830233 1103 60-61 2.0925076766322697 1103 62-63 2.275532195229122 1103 64-65 2.4082591409395846 1103 66-67 2.515072135563969 1103 68-69 2.66028066872806 1103 70-71 2.5825696426242963 1103 72-73 2.84802470173587 1103 74-75 2.941520984287127 1103 76-77 3.0927084648974343 1103 78-79 3.3098037644873273 1103 80-81 2.9633687580138286 1103 82-83 3.0635531102752154 1103 84-85 3.1266347291483214 1103 86-87 3.1998674749879896 1103 88-89 3.426813527939128 1103 90-91 3.6770014379864975 1103 92-93 3.7174927878672737 1103 94-95 3.5462201456539084 1103 96-97 3.288590584647242 1103 98-99 3.3154844662053318 1103 100-101 2.3932427930976763 1104 1 0.5175454116346145 1104 2 0.5625784938701521 1104 3 0.539013391543353 1104 4 0.4429879675580537 1104 5 0.4164760901948128 1104 6 0.5215131968465201 1104 7 0.5339072906808155 1104 8 0.5635316263330026 1104 9 0.7915376784334498 1104 10-11 0.7449992377866792 1104 12-13 0.7859141260890823 1104 14-15 0.782856809543766 1104 16-17 0.7920911858593769 1104 18-19 0.7791819220497302 1104 20-21 0.8432355161044214 1104 22-23 0.9408360227370807 1104 24-25 0.9850370952720624 1104 26-27 0.9585582042006919 1104 28-29 1.0414789626765995 1104 30-31 0.8262534194440363 1104 32-33 1.0118255992115621 1104 34-35 0.934307264371995 1104 36-37 1.201158713498117 1104 38-39 1.1178375001734366 1104 40-41 1.2331179933538365 1104 42-43 1.2727895201300754 1104 44-45 1.0730331328782867 1104 46-47 1.2843109091370764 1104 48-49 1.4252197718149873 1104 50-51 1.368734452796616 1104 52-53 1.5030259437362794 1104 54-55 1.813344112895713 1104 56-57 1.9158056845050133 1104 58-59 1.9274089943234571 1104 60-61 2.0827356279513474 1104 62-63 2.2776214305146745 1104 64-65 2.2912026800324092 1104 66-67 2.5286280753191726 1104 68-69 2.6066035939383703 1104 70-71 2.6300557716415547 1104 72-73 2.8915925823115494 1104 74-75 2.7121761702582994 1104 76-77 2.696060909858545 1104 78-79 2.9436726436735974 1104 80-81 3.1396495980108625 1104 82-83 3.1116244668850364 1104 84-85 3.3284791814248322 1104 86-87 3.6612600923664083 1104 88-89 3.7192556211659387 1104 90-91 3.723454572253168 1104 92-93 3.824892176900553 1104 94-95 4.055385844109161 1104 96-97 3.949395176228606 1104 98-99 3.6113686775923686 1104 100-101 2.544375777384942 1105 1 0.6646463061559125 1105 2 0.4432642965930498 1105 3 0.36118232484285073 1105 4 0.4479873242340986 1105 5 0.5074175073094338 1105 6 0.43288672563198105 1105 7 0.4954705974822673 1105 8 0.6165935575639665 1105 9 0.6683224177484846 1105 10-11 0.6109994494050639 1105 12-13 0.6733601871251764 1105 14-15 0.7303918788620507 1105 16-17 0.6618707854190404 1105 18-19 0.7363277206851464 1105 20-21 0.719677778519177 1105 22-23 0.6456346072407229 1105 24-25 0.7496284325298177 1105 26-27 0.6526074116980212 1105 28-29 0.7890391527494955 1105 30-31 0.8227807455090854 1105 32-33 0.9017597577341405 1105 34-35 0.9924001317816504 1105 36-37 0.9534484825249194 1105 38-39 1.161946974014029 1105 40-41 1.2292101957063082 1105 42-43 1.4552042062415538 1105 44-45 1.3752952456072194 1105 46-47 1.494636467790727 1105 48-49 1.445627425951784 1105 50-51 1.4651494292966731 1105 52-53 1.546826866959723 1105 54-55 1.8209223262427514 1105 56-57 1.8066913094643944 1105 58-59 1.83392963614547 1105 60-61 1.9732950504229514 1105 62-63 2.0164545051409704 1105 64-65 2.297035653951209 1105 66-67 2.491033060044181 1105 68-69 2.569661952961294 1105 70-71 2.5684575794379896 1105 72-73 2.6570361069881017 1105 74-75 2.8732334193560902 1105 76-77 2.9648737480946608 1105 78-79 3.0755692575856592 1105 80-81 3.1387043556440446 1105 82-83 3.476698567499273 1105 84-85 3.4288244540549115 1105 86-87 3.3822319227358086 1105 88-89 3.543609560150255 1105 90-91 3.5162797194549746 1105 92-93 3.469235961467737 1105 94-95 3.3115389239881594 1105 96-97 3.255072160668739 1105 98-99 2.92370153963096 1105 100-101 2.123604770204916 1106 1 0.2244943712451537 1106 2 0.21587883591221413 1106 3 0.10971267856420752 1106 4 0.3247327352111071 1106 5 0.23418538791082 1106 6 -0.10716189758429095 1106 7 0.26158297375929607 1106 8 -0.1213907946947117 1106 9 0.29521066177782274 1106 10-11 0.12476019831719753 1106 12-13 0.3183863203937918 1106 14-15 0.5691066203136543 1106 16-17 0.06837375147885183 1106 18-19 0.39203676497415074 1106 20-21 0.47348356185251106 1106 22-23 0.34022670945277156 1106 24-25 0.3850681704645069 1106 26-27 0.4169604357188561 1106 28-29 0.4476883818376507 1106 30-31 0.31367318664417354 1106 32-33 0.4041600370653242 1106 34-35 0.3231953344152316 1106 36-37 0.4846435383946286 1106 38-39 0.5418302509328896 1106 40-41 0.6936706159066262 1106 42-43 0.15064666452256148 1106 44-45 0.6215024493499541 1106 46-47 0.8803661099789366 1106 48-49 0.6414843801493433 1106 50-51 0.5849364194386908 1106 52-53 0.6801617567594214 1106 54-55 0.6415830491906434 1106 56-57 1.251919357067674 1106 58-59 1.2246111215616295 1106 60-61 1.3435942121815465 1106 62-63 1.3736337954934257 1106 64-65 1.3269640237826827 1106 66-67 1.4465622382864467 1106 68-69 1.6846514577184806 1106 70-71 1.41887650186559 1106 72-73 1.592046151153081 1106 74-75 1.9202621994106401 1106 76-77 2.08929152309922 1106 78-79 2.447932125215331 1106 80-81 2.257086957086244 1106 82-83 2.342914483488155 1106 84-85 2.3252557443427335 1106 86-87 2.0480845178361484 1106 88-89 1.9068598128777658 1106 90-91 2.208263501273681 1106 92-93 2.3484468350012744 1106 94-95 2.351125564192916 1106 96-97 2.376633472902732 1106 98-99 2.1350292738491596 1106 100-101 1.542002609155456 1107 1 0.22907690132846525 1107 2 0.25500213896187063 1107 3 0.1602716759294438 1107 4 0.2057889836762712 1107 5 0.23032950258740215 1107 6 0.18854572634818823 1107 7 0.27863131669455043 1107 8 0.3262161025644943 1107 9 0.28655224689779857 1107 10-11 0.2786085681011343 1107 12-13 0.29185912806908476 1107 14-15 0.41749575165513875 1107 16-17 0.3669101845420215 1107 18-19 0.43625356922605363 1107 20-21 0.4026362682025635 1107 22-23 0.44114224572241056 1107 24-25 0.4056368852654231 1107 26-27 0.4536979233914238 1107 28-29 0.49837192514498696 1107 30-31 0.504954077008847 1107 32-33 0.7336519837440676 1107 34-35 0.5966384221962642 1107 36-37 0.5251984067291318 1107 38-39 0.7452167216498111 1107 40-41 0.7411383544670116 1107 42-43 0.9093877218149302 1107 44-45 0.8319327536784371 1107 46-47 0.9152809486011435 1107 48-49 0.734367653886018 1107 50-51 1.0154702547206895 1107 52-53 1.1221518221764661 1107 54-55 1.1562295093715491 1107 56-57 1.3538343032177202 1107 58-59 1.3116584671786171 1107 60-61 1.4839451769419831 1107 62-63 1.5791403043499237 1107 64-65 1.6137137045980552 1107 66-67 1.7437707358944508 1107 68-69 1.7585701874031123 1107 70-71 1.9503007265457093 1107 72-73 1.9636176081514094 1107 74-75 2.2404494708724023 1107 76-77 2.1822414907125207 1107 78-79 2.52182428192987 1107 80-81 2.6727348902368284 1107 82-83 2.6367585293412183 1107 84-85 2.4943845226601766 1107 86-87 2.5588093495493887 1107 88-89 2.5682145951139397 1107 90-91 2.2266156310615806 1107 92-93 2.1714378162588215 1107 94-95 2.2974908049606597 1107 96-97 2.0412932861199486 1107 98-99 1.8718066542524934 1107 100-101 1.3420075909931404 1108 1 0.29822104774135383 1108 2 0.2759528283662043 1108 3 0.1793551311607935 1108 4 0.14967860127505617 1108 5 0.19085001491163212 1108 6 0.27425025491046284 1108 7 0.27233393206356027 1108 8 0.2649638783604047 1108 9 0.3785399496484345 1108 10-11 0.3169170433530226 1108 12-13 0.308276191608833 1108 14-15 0.29107146957030494 1108 16-17 0.42239957959448304 1108 18-19 0.3882570797450171 1108 20-21 0.38935553029564574 1108 22-23 0.38818185975959096 1108 24-25 0.48733789143670947 1108 26-27 0.5021883156931821 1108 28-29 0.4992385372963142 1108 30-31 0.5211993560839545 1108 32-33 0.6833817784733327 1108 34-35 0.7432735962436041 1108 36-37 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1.9061892133412783 2201 78-79 1.9670438312061762 2201 80-81 1.8630284080194173 2201 82-83 2.311468728635944 2201 84-85 2.5397173542927547 2201 86-87 2.6819775517705757 2201 88-89 2.3760516424793643 2201 90-91 2.326606343126457 2201 92-93 2.4745298326695373 2201 94-95 2.5898337494035815 2201 96-97 2.4546340916732206 2201 98-99 2.227017928130861 2201 100-101 1.4364871602004383 2202 1 0.47497051922845657 2202 2 0.5586792811162695 2202 3 0.5836316063836655 2202 4 0.4662170542732085 2202 5 0.5489696651781273 2202 6 0.6108261613072088 2202 7 0.6313676167689621 2202 8 0.5482106129125484 2202 9 0.6586723630898561 2202 10-11 0.6269334060051293 2202 12-13 0.7738152269091287 2202 14-15 0.8825934986616772 2202 16-17 0.9828633405695939 2202 18-19 0.9061132928438695 2202 20-21 0.8804849935593069 2202 22-23 0.868037545685489 2202 24-25 0.8322618035730187 2202 26-27 0.7737129653356405 2202 28-29 0.9936881469126817 2202 30-31 1.1085258713903485 2202 32-33 1.127333138824568 2202 34-35 1.005967716102134 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0.49760425039230327 2203 2 0.5342460469330526 2203 3 0.581339780793499 2203 4 0.5277434619396857 2203 5 0.5774181060087784 2203 6 0.5943730293472171 2203 7 0.518998493611285 2203 8 0.5700237392425436 2203 9 0.6259948683513414 2203 10-11 0.7095338512026572 2203 12-13 0.6686971491429361 2203 14-15 0.8623823111328477 2203 16-17 0.9235451755706521 2203 18-19 0.9102983875789121 2203 20-21 0.9900009042592757 2203 22-23 1.1573866010774694 2203 24-25 0.9639439759304906 2203 26-27 1.1090101948793532 2203 28-29 1.1346998471103191 2203 30-31 0.9427172655958387 2203 32-33 1.052730490425553 2203 34-35 1.3159717349384366 2203 36-37 1.3475113510930328 2203 38-39 1.4050139170966887 2203 40-41 1.4593074932407148 2203 42-43 1.398125386781814 2203 44-45 1.6485283366484396 2203 46-47 1.6887864145104317 2203 48-49 1.8319705308618879 2203 50-51 1.7858802732394636 2203 52-53 1.8460001876525318 2203 54-55 1.7978923088804635 2203 56-57 1.7998302742994063 2203 58-59 1.937988373095358 2203 60-61 2.0502994928510994 2203 62-63 2.411249743050849 2203 64-65 2.4279626539280876 2203 66-67 2.535691041684462 2203 68-69 2.483831289240836 2203 70-71 2.574030225477653 2203 72-73 2.8097885629891337 2203 74-75 2.7242036185530445 2203 76-77 2.8082575078514367 2203 78-79 2.9437413929328073 2203 80-81 3.0293111680521783 2203 82-83 2.973578810440795 2203 84-85 2.9299531900227755 2203 86-87 2.801027693349784 2203 88-89 2.9051945208228247 2203 90-91 2.840516659362354 2203 92-93 2.8453837402240243 2203 94-95 2.7203375023637335 2203 96-97 2.6030460474979655 2203 98-99 2.6525632541122324 2203 100-101 1.697355884368581 2204 1 0.3709003147182699 2204 2 0.3859693886896167 2204 3 0.4464248468752423 2204 4 0.3059197916110321 2204 5 0.32879071950763716 2204 6 0.4659786168996476 2204 7 0.49821814558841737 2204 8 0.5248144664585155 2204 9 0.44928348898215376 2204 10-11 0.5313558889715395 2204 12-13 0.4609598458906774 2204 14-15 0.5017766781019688 2204 16-17 0.6191494500785808 2204 18-19 0.6076811804766535 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2.2796442699730086 2204 88-89 2.1584386503127995 2204 90-91 2.3885007978780557 2204 92-93 2.1949064302782784 2204 94-95 2.1107798172246497 2204 96-97 2.2111340401374537 2204 98-99 2.074169288675078 2204 100-101 1.5083698905653113 2205 1 0.08827524803649567 2205 2 0.27024731360003784 2205 3 0.42159647277751233 2205 4 0.2659106331940251 2205 5 0.2877958510536658 2205 6 0.4490227047637987 2205 7 0.4461215762181254 2205 8 0.3746377656602249 2205 9 0.525877029937746 2205 10-11 0.5413590991383259 2205 12-13 0.4480924116164786 2205 14-15 0.5761611050163822 2205 16-17 0.677566507394225 2205 18-19 0.597860005650027 2205 20-21 0.612444036189359 2205 22-23 0.5279599118283116 2205 24-25 0.5386340983269804 2205 26-27 0.49985128095033815 2205 28-29 0.5528189905747567 2205 30-31 0.7446044554663374 2205 32-33 0.8046769833413379 2205 34-35 0.8196841247648052 2205 36-37 0.8711145868514194 2205 38-39 0.9559216951036973 2205 40-41 1.1688872860638924 2205 42-43 1.2552861815193879 2205 44-45 1.2903191830602694 2205 46-47 1.3339654031919963 2205 48-49 1.275799463646976 2205 50-51 1.1858093590801388 2205 52-53 1.311142471779366 2205 54-55 1.2876513542741677 2205 56-57 1.3386942083559497 2205 58-59 1.3086779389260563 2205 60-61 1.4676903987578918 2205 62-63 1.4909320687282026 2205 64-65 1.4377419468016477 2205 66-67 1.565097051635476 2205 68-69 1.5064136585798202 2205 70-71 1.5896249112338872 2205 72-73 1.671381636623746 2205 74-75 1.7052927602422194 2205 76-77 1.5016156147600483 2205 78-79 1.5463268329796342 2205 80-81 1.4822531940476367 2205 82-83 1.701081989103212 2205 84-85 1.8969508534060253 2205 86-87 1.805335848478105 2205 88-89 1.631076557661725 2205 90-91 1.4657034207731527 2205 92-93 1.5557427731429758 2205 94-95 1.6783863074265604 2205 96-97 1.502941883330248 2205 98-99 1.5348067900323183 2205 100-101 0.6066729397516761 2206 1 0.20959699093311457 2206 2 0.36069989143650716 2206 3 0.3372981249561313 2206 4 0.3100316878201923 2206 5 0.31376906876509025 2206 6 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1.0314206142541664 2206 70-71 1.08338015152831 2206 72-73 1.305699545072482 2206 74-75 1.2894544425974068 2206 76-77 1.537375030812946 2206 78-79 1.4266243145782873 2206 80-81 1.512116128424207 2206 82-83 1.2667145553115837 2206 84-85 1.329281298545645 2206 86-87 1.0572873682517603 2206 88-89 1.2366183117156702 2206 90-91 1.637698298760501 2206 92-93 1.3085632461288288 2206 94-95 1.042164497915529 2206 96-97 0.9086892711273364 2206 98-99 0.9743504695487388 2206 100-101 0.4677043097252893 2207 1 0.24931014315333755 2207 2 0.4115637253642177 2207 3 0.3006170537933457 2207 4 0.20728252109087464 2207 5 0.3718196239701612 2207 6 0.3850731098204889 2207 7 0.2858532767369226 2207 8 0.3065788908276872 2207 9 0.3810590581883133 2207 10-11 0.4049748032895977 2207 12-13 0.42435424637493213 2207 14-15 0.4425869405301057 2207 16-17 0.5456459722338636 2207 18-19 0.475300730705996 2207 20-21 0.6620099376989899 2207 22-23 0.5014625058095561 2207 24-25 0.5868465440421886 2207 26-27 0.5081245076472172 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-0.6448098990667823 2315 98-99 -0.6577795979626782 2315 100-101 -0.14513523708530318 2316 1 -0.9373686820299909 2316 2 -0.8627767960012669 2316 3 -0.756737121724516 2316 4 -0.7940822033218353 2316 5 -0.6591818302160632 2316 6 -0.6355834155379583 2316 7 -0.9264472044225585 2316 8 -0.6662223001542813 2316 9 -0.9545966554509846 2316 10-11 -0.9453492798995242 2316 12-13 -0.982021980270865 2316 14-15 -1.2216538084621646 2316 16-17 -1.2584015703295819 2316 18-19 -1.128453038606871 2316 20-21 -1.061842861350435 2316 22-23 -1.190166165492549 2316 24-25 -1.0999253697843159 2316 26-27 -1.3318010214382383 2316 28-29 -1.3904788984360152 2316 30-31 -1.2029953296291325 2316 32-33 -1.488840958947847 2316 34-35 -1.2238729928282694 2316 36-37 -1.5820521208560336 2316 38-39 -1.706078260124528 2316 40-41 -1.6611597243007736 2316 42-43 -1.3000978483881553 2316 44-45 -1.610645424850901 2316 46-47 -1.2385615158382421 2316 48-49 -1.1046010198882037 2316 50-51 -1.1451115963495546 2316 52-53 -1.303754665065565 2316 54-55 -1.413232216722676 2316 56-57 -1.330254550346563 2316 58-59 -0.9882661635117671 2316 60-61 -1.0707099056901832 2316 62-63 -1.123250958291564 2316 64-65 -0.9505817979096882 2316 66-67 -0.6799966333055387 2316 68-69 -0.7162372782695918 2316 70-71 -0.8103797898025782 2316 72-73 -0.6035638537391286 2316 74-75 -0.39795050720198333 2316 76-77 -0.5393223539444811 2316 78-79 -0.4593470941766853 2316 80-81 -0.4078965675426538 2316 82-83 -0.6109255773269133 2316 84-85 -0.569273766593529 2316 86-87 -0.27692803636888286 2316 88-89 -0.2949517381006288 2316 90-91 -0.28069245126911113 2316 92-93 -0.3131361415891778 2316 94-95 -0.10238175238853131 2316 96-97 -0.018852793305601523 2316 98-99 -0.0853904296404 2316 100-101 0.2429051709531267 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12091.0 3 4750.0 4 5208.0 5 5953.0 6 7129.0 7 9017.0 8 11724.0 9 15256.0 10 18989.0 11 23116.0 12 28538.0 13 33114.0 14 37542.0 15 42663.0 16 48216.0 17 53911.0 18 54451.0 19 55875.0 20 60642.0 21 66083.0 22 73166.0 23 80691.0 24 88744.0 25 97850.0 26 107502.0 27 118111.0 28 129860.0 29 142362.0 30 157141.0 31 174241.0 32 193969.0 33 216608.0 34 239077.0 35 254504.0 36 243359.0 37 165027.0 38 40400.0 39 572.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.2301991197462 18.442115648913465 14.410968008686615 23.91671722265372 2 15.414337571027993 19.65838540207723 40.16386052154415 24.763416505350623 3 19.260848470535084 23.272260414634395 29.799280191401316 27.667610923429205 4 12.844259867159552 16.228491731088113 36.78814557700744 34.139102824744896 5 13.664600659282108 37.28442534288126 34.44614204415971 14.604831953676925 6 33.03159012306373 35.80922169187595 17.040237705822996 14.118950479237327 7 28.854200260244937 32.56989469867263 20.309405881022176 18.266499160060263 8 26.57635686129035 34.63579138422122 19.744332585850366 19.043519168638063 9 26.32207384636119 16.66101365867277 19.98495027010896 37.03196222485708 10-11 24.990966758956546 26.207213243533612 27.531633992968512 21.27018600454133 12-13 26.115467078576195 23.547941917676706 27.47575678428207 22.860834219465033 14-15 22.39590477524345 25.185606583829745 25.78017975005178 26.638308890875024 16-17 22.24587031746057 28.277540975979903 26.924150521454138 22.552438185105387 18-19 22.38725419338982 26.74154844628035 28.89604269265737 21.975154667672463 20-21 22.60522229162231 26.076465706078878 29.18517432266592 22.133137679632895 22-23 22.320189024676885 26.09155349321665 29.073710735576753 22.51454674652971 24-25 22.295378359175988 26.269155751009414 29.16946658169045 22.265999308124147 26-27 21.739244141317858 26.60308025144268 28.87186605169243 22.785809555547036 28-29 21.99120062545093 27.243358394260948 28.62349294707171 22.141948033216412 30-31 22.086459093263954 26.57967565205156 28.519894345209686 22.813970909474804 32-33 21.550846314193915 26.608548861904225 28.63431875730276 23.2062860665991 34-35 22.673682650072855 26.803616104332324 28.335669819666464 22.187031425928357 36-37 22.871270258718614 26.08299993967468 28.378554862028295 22.667174939578416 38-39 22.048183706818794 26.633812073373264 28.38391108791192 22.934093131896027 40-41 22.735830488632935 26.422870188144028 28.46317844720984 22.378120876013195 42-43 22.370783701593915 26.44544938613255 28.2217814908434 22.961985421430132 44-45 22.330922322372675 26.731183078565635 28.554182621728998 22.38371197733269 46-47 22.952301048497837 27.017139153758297 27.584300124485058 22.446259673258815 48-49 22.389394258522568 27.118893662661055 27.577070985722905 22.914641093093476 50-51 22.69040928980498 27.26259983455747 27.64162267136912 22.40536820426843 52-53 23.112421348717415 26.773508526908213 27.666131510991647 22.44793861338272 54-55 22.25255036571282 27.166078532015913 28.007602977030672 22.5737681252406 56-57 22.494413601826913 27.23262241594498 27.61700959797021 22.6559543842579 58-59 22.465571849972036 26.709033111877474 27.67838969655238 23.14700534159811 60-61 21.863753003075495 27.06004803637599 28.20371093355267 22.87248802699584 62-63 22.15725772171261 27.00601555234102 28.319645874497567 22.517080851448803 64-65 21.792832053000996 27.187037699722943 27.941378620628704 23.07875162664736 66-67 21.781389908144313 27.12738170145626 27.919773152424565 23.171455237974868 68-69 22.649052043646687 26.616496514485405 27.96944815452424 22.76500328734367 70-71 22.288948674992092 26.795294122176244 27.540649167575115 23.37510803525655 72-73 22.211198850731982 26.793545761633077 27.74041505372998 23.254840333904962 74-75 22.298003737478243 27.22236391488816 27.519228764145875 22.960403583487725 76-77 22.340954497569037 26.67782274569786 27.718472234162817 23.26275052257029 78-79 22.456915910636603 26.974473559265483 27.527797738967525 23.04081279113039 80-81 22.806760331853802 26.897186820230562 27.167695054578857 23.12835779333678 82-83 22.52149041258673 26.954958238284238 27.430603399807413 23.092947949321623 84-85 22.45409932174545 27.010507050815757 27.407643255910568 23.127750371528226 86-87 22.827384733037956 26.954723401634457 26.95526873276593 23.262623132561657 88-89 22.519309772870553 27.04919400246074 26.746221059402714 23.68527516526599 90-91 22.419473401010894 26.992778443202152 26.928253847652005 23.659494308134942 92-93 22.728385350332776 26.42621944216492 27.261445012418505 23.583950195083798 94-95 22.296540172680952 26.21300739527241 27.429515990578125 24.060936441468517 96-97 22.6774175022611 25.739345870164136 27.416202869377766 24.167033758196997 98-99 22.391095436990522 26.069612493798317 26.817214791255395 24.722077277955766 100-101 23.209747358393546 24.993118767345603 27.289266465416706 24.50786740884414 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 158.0 1 123.0 2 122.5 3 181.0 4 179.0 5 177.5 6 289.0 7 582.0 8 983.0 9 1200.0 10 1156.0 11 1086.0 12 1117.5 13 1233.0 14 1438.0 15 1714.0 16 1953.0 17 2166.5 18 2527.0 19 3040.5 20 3738.0 21 4609.0 22 5728.5 23 7211.0 24 9431.5 25 12336.0 26 15876.0 27 20457.5 28 25098.5 29 30004.0 30 35735.5 31 44573.0 32 56093.5 33 60544.5 34 60775.5 35 64750.5 36 70959.0 37 77049.0 38 81996.0 39 86869.0 40 92267.0 41 98508.0 42 102711.5 43 105166.5 44 107740.0 45 112565.5 46 116197.0 47 118629.5 48 120730.0 49 118269.0 50 113922.0 51 110080.0 52 106982.0 53 105715.0 54 105026.5 55 99799.5 56 92144.5 57 84291.5 58 77823.0 59 68871.5 60 55090.0 61 45417.5 62 39572.5 63 31220.0 64 22576.5 65 16968.5 66 12936.0 67 10160.5 68 7944.5 69 6068.5 70 4693.5 71 3374.5 72 2380.0 73 1785.0 74 1368.0 75 991.5 76 729.5 77 530.5 78 354.0 79 240.5 80 158.5 81 97.0 82 58.5 83 31.5 84 15.5 85 15.0 86 14.5 87 10.5 88 6.5 89 4.0 90 3.5 91 4.5 92 2.5 93 1.5 94 1.0 95 1.0 96 0.5 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38441650424769974 2 0.02883765331430925 3 0.03425874720765548 4 0.04436315298519432 5 0.062294463555493396 6 0.053505234402967554 7 0.03711364280829344 8 0.039006214049165794 9 0.0358305436619393 10-11 0.037979734732082486 12-13 0.029190505579556637 14-15 0.027153585684719442 16-17 0.02203722783863232 18-19 0.027281895599354858 20-21 0.03405024359637294 22-23 0.03315207419392504 24-25 0.041251637555285534 26-27 0.03578242744395102 28-29 0.031451967825005804 30-31 0.034531405776255734 32-33 0.02914238936156836 34-35 0.03044152724725192 36-37 0.03259071831739511 38-39 0.015573615888873349 40-41 0.012782875245553098 42-43 0.013183843728788768 44-45 0.011676202231822655 46-47 0.01963141693921831 48-49 0.017209567300474876 50-51 0.011467698620540107 52-53 0.009318507550396928 54-55 0.008083524622031069 56-57 0.0090618877211261 58-59 0.0040578010503449615 60-61 0.007409897570195147 62-63 0.011708279710481509 64-65 0.007297626394889159 66-67 0.003737026263756427 68-69 0.006206992120488143 70-71 0.013280076164765328 72-73 0.010826149047363039 74-75 0.01209320945438775 76-77 0.00538901641468738 78-79 0.009366623768385207 80-81 0.011435621141881253 82-83 0.013552734733365582 84-85 0.008324105711972469 86-87 0.002566198292708276 88-89 0.0162632816800387 90-91 0.026014835192330145 92-93 0.010585567957421637 94-95 0.02128340709014926 96-97 0.019535184503241747 98-99 0.011339388705904693 100-101 0.12545501903477582 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3117452.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.39331021312182 #Duplication Level Percentage of deduplicated Percentage of total 1 75.75518623695827 40.448201590027296 2 13.32121750204765 14.225277970065958 3 4.718163337808851 7.557550761954188 4 2.0987146948018736 4.482292989935748 5 1.164407266675402 3.1085779202006494 6 0.6799840892652216 2.178396085087506 7 0.4600123059341916 1.7193105826818453 8 0.31816309422879074 1.3590224630819627 9 0.2438596540238076 1.1718426740181949 >10 1.1178737853702885 10.465709032845973 >50 0.07381551620173757 2.7254864925013518 >100 0.04066721667104776 4.141306850791956 >500 0.004058512221048473 1.4348602302215403 >1k 0.003573913745482668 4.054990562359294 >5k 3.028740462273448E-4 0.9271737942265514 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6779 0.21745322782836754 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 5690 0.18252085356887612 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5665 0.1817189166024048 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5378 0.17251268022731386 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5155 0.16535940248638953 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4928 0.15807781483082978 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4330 0.13889548259283543 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4219 0.1353348824617027 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4190 0.13440463558059595 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3993 0.12808537228480182 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3710 0.11900744582434629 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3603 0.11557515560784896 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3337 0.10704254628459396 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3261 0.10460465790652108 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.41549573177069E-5 2 0.0 0.0 0.0 0.0 6.41549573177069E-5 3 0.0 0.0 0.0 0.0 6.41549573177069E-5 4 0.0 0.0 0.0 3.207747865885345E-5 6.41549573177069E-5 5 0.0 0.0 0.0 6.41549573177069E-5 6.41549573177069E-5 6 0.0 0.0 0.0 6.41549573177069E-5 6.41549573177069E-5 7 0.0 0.0 0.0 6.41549573177069E-5 6.41549573177069E-5 8 0.0 0.0 0.0 6.41549573177069E-5 6.41549573177069E-5 9 0.0 0.0 0.0 6.41549573177069E-5 6.41549573177069E-5 10-11 1.6038739329426725E-5 0.0 0.0 9.623243597656035E-5 9.623243597656035E-5 12-13 3.207747865885345E-5 0.0 0.0 9.623243597656035E-5 2.88697307929681E-4 14-15 3.207747865885345E-5 0.0 0.0 9.623243597656035E-5 3.528522652473879E-4 16-17 3.207747865885345E-5 0.0 0.0 9.623243597656035E-5 4.4908470122394825E-4 18-19 4.8116217988280175E-5 0.0 0.0 9.623243597656035E-5 4.8116217988280173E-4 20-21 6.41549573177069E-5 0.0 0.0 1.4434865396484054E-4 5.77394615859362E-4 22-23 9.623243597656035E-5 0.0 0.0 2.2454235061197415E-4 7.217432698242026E-4 24-25 1.283099146354138E-4 0.0 0.0 4.972009192122284E-4 7.377820091536293E-4 26-27 1.283099146354138E-4 0.0 0.0 8.660919237890431E-4 7.377820091536293E-4 28-29 2.566198292708276E-4 0.0 0.0 0.002983205515273371 7.377820091536293E-4 30-31 2.566198292708276E-4 0.0 0.0 0.011371466184563547 7.377820091536293E-4 32-33 2.566198292708276E-4 0.0 0.0 0.032285982270135995 7.698594878124828E-4 34-35 2.886973079296811E-4 0.0 0.0 0.061829340114940026 8.660919237890431E-4 36-37 3.207747865885345E-4 0.0 0.0 0.11281649244318757 8.660919237890431E-4 38-39 3.207747865885345E-4 0.0 0.0 0.18811837359484607 9.142081417773233E-4 40-41 3.207747865885345E-4 0.0 0.0 0.2713433919752413 9.3024688110675E-4 42-43 3.6889100457681466E-4 0.0 0.0 0.35525807614680194 9.462856204361767E-4 44-45 4.170072225650948E-4 0.0 0.0 0.4661499198704583 0.0010104405777538836 46-47 4.8116217988280173E-4 0.0 0.0 0.5986940616888408 0.0010906342744010171 48-49 5.132396585416552E-4 0.0 0.0 0.7347988036383559 0.001154789231718724 50-51 5.132396585416552E-4 0.0 0.0 0.8896528318639709 0.001218944189036431 52-53 5.292783978710819E-4 0.0 0.0 1.036054444462978 0.0013151766250129914 54-55 5.453171372005086E-4 3.207747865885345E-5 0.0 1.1957682107054093 0.0013472541036718447 56-57 5.453171372005086E-4 3.207747865885345E-5 0.0 1.3843517077408087 0.0013472541036718447 58-59 5.453171372005086E-4 3.207747865885345E-5 0.0 1.5816602789714165 0.0013472541036718447 60-61 5.453171372005086E-4 3.207747865885345E-5 0.0 1.8020325573577396 0.0013632928430012715 62-63 5.453171372005086E-4 3.207747865885345E-5 0.0 2.014064691292761 0.0015236802362955388 64-65 5.453171372005086E-4 3.207747865885345E-5 0.0 2.2378692598955814 0.0015397189756249656 66-67 5.453171372005086E-4 3.207747865885345E-5 0.0 2.4979534568615653 0.0015397189756249656 68-69 5.453171372005086E-4 3.207747865885345E-5 0.0 2.7884631423354715 0.0015557577149543922 70-71 5.613558765299354E-4 3.207747865885345E-5 0.0 3.0877620569619033 0.001635951411601526 72-73 5.77394615859362E-4 3.207747865885345E-5 0.0 3.4056819479498 0.001796338804895793 74-75 5.77394615859362E-4 3.207747865885345E-5 0.0 3.716352328760796 0.0019406874588606337 76-77 5.77394615859362E-4 3.207747865885345E-5 0.0 4.056213215151348 0.001972764937519487 78-79 5.934333551887887E-4 3.207747865885345E-5 0.0 4.4375823589264565 0.0019888036768489136 80-81 6.094720945182155E-4 3.207747865885345E-5 0.0 4.83001823283887 0.002052958634166621 82-83 6.094720945182155E-4 3.207747865885345E-5 0.0 5.237963567682838 0.002149191070143181 84-85 6.41549573177069E-4 3.207747865885345E-5 0.0 5.650688446846976 0.0021652298094726077 86-87 6.41549573177069E-4 3.207747865885345E-5 0.0 6.078377469805469 0.002261462245449168 88-89 6.41549573177069E-4 3.207747865885345E-5 0.0 6.515834085015583 0.002357694681425729 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7000 0.0 33.786175 1 GTATCAA 10915 0.0 33.20055 1 ATCAACG 12905 0.0 27.764845 3 TCAACGC 12970 0.0 27.625702 4 TATCAAC 13020 0.0 27.556108 2 CAACGCA 13030 0.0 27.389082 5 AACGCAG 13580 0.0 26.379278 6 GTACATG 9750 0.0 25.626074 1 TACATGG 9785 0.0 25.545057 2 ACATGGG 9950 0.0 23.736423 3 ACGCAGA 15100 0.0 23.688995 7 TAAGGTG 2275 0.0 23.394758 5 CGCAGAG 15335 0.0 23.202063 8 AGGTAAG 2125 0.0 22.362635 2 GTGTAAG 1550 0.0 21.226227 1 GAGTACT 8840 0.0 21.010633 12-13 GCAGAGT 16285 0.0 20.885933 9 AGAGTAC 12685 0.0 20.483847 10-11 GTAAGGT 2495 0.0 20.379578 4 GGTAAGG 2435 0.0 20.101118 3 >>END_MODULE