FastQCFastQC Report
Thu 26 May 2016
SRR938472_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938472_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3117452
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66350.21283407090149264No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA58870.18884011686467025No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA58670.18819856729149317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57980.18598522126403227No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA57120.18322655809937088No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC52480.1683426080016629No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA49780.15968168876377248No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG47430.1521434812789419No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA39490.12667396322381227No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA39180.1256795613853878No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA39050.12526255416282273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38610.12385114510183318No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG37270.11955276296154681No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA35420.1136184294096589No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT31570.10126860012600035No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66750.037.5862051
GTATCAA103450.032.15241
TAAGGTG22900.025.7192255
ATCAACG127000.025.6196083
TATCAAC127850.025.600762
TCAACGC128650.025.3271334
CAACGCA129400.025.1803365
AACGCAG134650.024.163286
CGTAACC1201.7119452E-423.7495633
GTAAGGT25250.022.7612274
GTACATG94350.022.411141
TAAGGTA13450.022.2479484
AAGGTAA23050.021.8514771
GGTAAGG26050.021.6982573
TACATGG92350.021.6046262
AGGTAAG25100.021.5757312
ACGCAGA152400.021.2554877
GTGTAAG15450.021.221031
CGCAGAG154500.020.9050948
ACATGGG91750.020.8633773
Produced by FastQC (version 0.11.5)