##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938472_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3117452 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.761379806329014 31.0 31.0 34.0 30.0 34.0 2 32.01673257519282 31.0 31.0 34.0 30.0 34.0 3 32.07754185148641 33.0 31.0 34.0 30.0 34.0 4 35.74658374852283 37.0 35.0 37.0 33.0 37.0 5 35.63246202347302 37.0 35.0 37.0 33.0 37.0 6 35.6642014055068 37.0 35.0 37.0 33.0 37.0 7 35.57869631994334 37.0 35.0 37.0 33.0 37.0 8 35.54347524837592 37.0 35.0 37.0 33.0 37.0 9 37.08397915990366 39.0 37.0 39.0 33.0 39.0 10-11 37.006848541693664 39.0 37.0 39.0 33.0 39.0 12-13 36.89493037904032 39.0 37.0 39.0 33.0 39.0 14-15 37.907525761423116 40.0 37.0 41.0 33.0 41.0 16-17 37.99109176340165 40.0 37.0 41.0 33.0 41.0 18-19 38.12562631277081 40.0 37.0 41.0 33.0 41.0 20-21 38.07221249918202 40.0 37.0 41.0 33.0 41.0 22-23 37.95433514293083 40.0 37.0 41.0 33.0 41.0 24-25 37.83350040353468 40.0 37.0 41.0 32.0 41.0 26-27 37.61741367629718 40.0 37.0 41.0 32.0 41.0 28-29 37.477903428825854 39.0 36.5 41.0 32.0 41.0 30-31 37.21000692231989 39.0 36.0 41.0 31.0 41.0 32-33 37.09492944879344 39.0 36.0 41.0 31.0 41.0 34-35 36.961606145018436 39.0 36.0 41.0 30.5 41.0 36-37 36.82842911454611 39.0 35.5 41.0 30.0 41.0 38-39 36.60390969933138 39.0 35.0 40.0 30.0 41.0 40-41 36.38405033982881 39.0 35.0 40.0 30.0 41.0 42-43 36.11307952776819 38.5 35.0 40.0 29.5 41.0 44-45 35.828474824953204 38.0 35.0 40.0 29.0 41.0 46-47 35.43789671821732 38.0 34.0 40.0 27.5 41.0 48-49 35.02615549493625 38.0 34.0 40.0 26.0 41.0 50-51 34.61828554216713 38.0 33.0 40.0 25.0 41.0 52-53 34.00897030651956 37.0 33.0 40.0 23.5 41.0 54-55 33.57099628157867 37.0 32.0 40.0 23.0 41.0 56-57 33.17908856335238 36.5 32.0 39.0 22.0 40.5 58-59 32.635910833591026 36.0 31.0 39.0 19.5 40.0 60-61 32.13507216791149 35.5 31.0 39.0 18.0 40.0 62-63 31.63255969939553 35.0 30.0 38.5 14.5 40.0 64-65 30.979390219961687 34.5 29.0 38.0 9.5 40.0 66-67 30.32728410894538 34.0 28.0 38.0 7.5 40.0 68-69 29.60208994396706 34.0 27.5 37.0 4.5 39.0 70-71 28.81810962927416 33.5 26.0 36.0 2.0 39.0 72-73 28.23367865808359 33.0 26.0 36.0 2.0 38.5 74-75 27.52547833936176 32.0 25.0 35.0 2.0 38.0 76-77 26.396092546092127 30.5 24.0 34.0 2.0 36.0 78-79 26.88704124393896 32.0 25.0 35.0 2.0 36.5 80-81 26.50903831077431 32.0 24.0 35.0 2.0 36.0 82-83 25.823090620160308 31.5 22.0 34.5 2.0 36.0 84-85 25.03237034603901 31.0 19.0 34.0 2.0 35.0 86-87 24.136968267675012 30.5 15.0 34.0 2.0 35.0 88-89 23.140668565225702 29.5 6.5 34.0 2.0 35.0 90-91 22.284622986977826 29.0 2.0 34.0 2.0 35.0 92-93 21.21227544161065 29.0 2.0 33.5 2.0 35.0 94-95 20.029675837831665 26.5 2.0 33.0 2.0 34.5 96-97 18.6470380297756 25.0 2.0 32.5 2.0 34.0 98-99 16.864385401924395 12.5 2.0 32.0 2.0 34.0 100-101 12.643460428580777 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28369653219487745 1101 2 0.2661797292948975 1101 3 0.2739640399585497 1101 4 0.18740432404718632 1101 5 0.15923070072803114 1101 6 0.22704702447681768 1101 7 0.20929313530601235 1101 8 0.2420257154047576 1101 9 0.315932009058443 1101 10-11 0.3018578089757611 1101 12-13 0.28373426458406925 1101 14-15 0.37357840738865633 1101 16-17 0.32269316146146565 1101 18-19 0.2546767086721218 1101 20-21 0.37164253370311684 1101 22-23 0.3542635338761144 1101 24-25 0.38209459947684365 1101 26-27 0.38039711142958055 1101 28-29 0.3976048765119984 1101 30-31 0.48373969343924017 1101 32-33 0.46388540284711866 1101 34-35 0.32484877833830694 1101 36-37 0.4012878549618719 1101 38-39 0.5246537056926499 1101 40-41 0.5005775921439835 1101 42-43 0.6144865550910481 1101 44-45 0.6340815622288929 1101 46-47 0.7881965753074525 1101 48-49 0.7673695170775616 1101 50-51 0.8869258310817898 1101 52-53 0.8962457815710536 1101 54-55 0.8660471061934558 1101 56-57 1.009997154033563 1101 58-59 1.027708232291502 1101 60-61 1.1774454080069532 1101 62-63 1.2705098220816957 1101 64-65 1.198742088291631 1101 66-67 1.274831478221504 1101 68-69 1.540287742006715 1101 70-71 1.7265108039063506 1101 72-73 1.8209785393859015 1101 74-75 1.8355218838098537 1101 76-77 1.758297284827261 1101 78-79 1.6055090252141753 1101 80-81 1.9450358886274515 1101 82-83 2.0651930053420635 1101 84-85 2.190712928150891 1101 86-87 2.130801258339467 1101 88-89 2.137708823826703 1101 90-91 2.5660981460211936 1101 92-93 2.7976748950454535 1101 94-95 2.6775043192029884 1101 96-97 2.756684275583236 1101 98-99 2.8136283612681616 1101 100-101 2.1500397956616535 1102 1 0.2601603251596778 1102 2 0.24791937967470545 1102 3 0.21500589835451933 1102 4 0.22805273863298936 1102 5 0.23411787337352052 1102 6 0.23165887345346192 1102 7 0.17956970516632964 1102 8 0.25917001826408637 1102 9 0.2874873359626875 1102 10-11 0.3037911104567499 1102 12-13 0.36994279286015086 1102 14-15 0.5320750936949779 1102 16-17 0.4252829354493528 1102 18-19 0.3896309569858829 1102 20-21 0.3733957840291211 1102 22-23 0.46237199626863656 1102 24-25 0.3664733542164882 1102 26-27 0.4035665149365073 1102 28-29 0.3432239609233605 1102 30-31 0.38019434923409534 1102 32-33 0.36873950674374356 1102 34-35 0.3968016403228347 1102 36-37 0.45144553345294014 1102 38-39 0.5119237422178102 1102 40-41 0.6341118287858762 1102 42-43 0.6625006078985294 1102 44-45 0.5155769238101726 1102 46-47 0.39264370363554946 1102 48-49 0.6493032206316514 1102 50-51 0.7892449934153873 1102 52-53 0.7429985005022104 1102 54-55 0.6835951515773999 1102 56-57 0.7357590237722249 1102 58-59 0.8253979814025953 1102 60-61 0.8647676199827075 1102 62-63 0.8916095954629562 1102 64-65 1.0503581282337109 1102 66-67 1.1431323760907048 1102 68-69 1.2670158216162442 1102 70-71 1.4875745153279105 1102 72-73 1.5210873812790062 1102 74-75 1.2498844286551112 1102 76-77 1.4451015631671815 1102 78-79 1.6346272883148245 1102 80-81 1.8582762145370921 1102 82-83 1.9403915142386978 1102 84-85 1.7770668258381015 1102 86-87 1.9340959299539087 1102 88-89 1.5569546757725732 1102 90-91 1.9025241111964917 1102 92-93 2.1123705727361326 1102 94-95 1.9786246218253076 1102 96-97 2.1147926204105296 1102 98-99 2.0712454127415114 1102 100-101 2.140693418387297 1103 1 0.22669036883345228 1103 2 0.2688339865946858 1103 3 0.23508805571134417 1103 4 0.17469336409906333 1103 5 0.22338918630801174 1103 6 0.23816014851470868 1103 7 0.26223701766211605 1103 8 0.24371096179564233 1103 9 0.30129709599785315 1103 10-11 0.3452104667905971 1103 12-13 0.48885131794217074 1103 14-15 0.5307849460154941 1103 16-17 0.3464663905372305 1103 18-19 0.3830597566202343 1103 20-21 0.34547923916289136 1103 22-23 0.47033998183706416 1103 24-25 0.4949267693935937 1103 26-27 0.5064579004602408 1103 28-29 0.43002788004450565 1103 30-31 0.4677451899209686 1103 32-33 0.47267719050561396 1103 34-35 0.5284290562736516 1103 36-37 0.6138249395501987 1103 38-39 0.5978950396872804 1103 40-41 0.5948344583352565 1103 42-43 0.6985814339785179 1103 44-45 0.7610066540992619 1103 46-47 0.8285308039255312 1103 48-49 0.8607327898458834 1103 50-51 0.9285326432872907 1103 52-53 1.0468139669929002 1103 54-55 1.1224880074254955 1103 56-57 1.26303740702118 1103 58-59 1.3361064767477089 1103 60-61 1.2722809361928498 1103 62-63 1.3244378795659664 1103 64-65 1.5220233508864425 1103 66-67 1.3990772889473675 1103 68-69 1.4442374041790451 1103 70-71 1.5354277453836538 1103 72-73 1.426066636580824 1103 74-75 1.4113558956891445 1103 76-77 1.5333846405322191 1103 78-79 1.6354253775356113 1103 80-81 1.8415171439803366 1103 82-83 2.1745811677258295 1103 84-85 2.2998018999118877 1103 86-87 2.3860851815340247 1103 88-89 2.1485103606367204 1103 90-91 2.310682415202116 1103 92-93 2.54903912702272 1103 94-95 2.332144933548758 1103 96-97 2.31630260544571 1103 98-99 2.4355360569537616 1103 100-101 2.3159981068588777 1104 1 0.2004764189892505 1104 2 0.20601222710330092 1104 3 0.222082610971583 1104 4 0.16267698121061613 1104 5 0.16119664174824067 1104 6 0.11800078369189038 1104 7 0.1549007243292735 1104 8 0.20012048871289778 1104 9 0.209221578447071 1104 10-11 0.23383468763491067 1104 12-13 0.24054877737936664 1104 14-15 0.36425073132396335 1104 16-17 0.32962315934489794 1104 18-19 0.437869832029115 1104 20-21 0.36196512766522204 1104 22-23 0.3621889428158269 1104 24-25 0.3654904312132672 1104 26-27 0.5117190148576967 1104 28-29 0.26683164022161776 1104 30-31 0.3425657700290472 1104 32-33 0.3951299071103165 1104 34-35 0.45715666478545813 1104 36-37 0.41134305193589427 1104 38-39 0.40911210511986695 1104 40-41 0.5945969920321232 1104 42-43 0.5687678825899312 1104 44-45 0.572460859196326 1104 46-47 0.8032108640836597 1104 48-49 0.7935980935365379 1104 50-51 0.761124071352576 1104 52-53 0.8569328188231822 1104 54-55 0.9630459889989353 1104 56-57 1.0915716563364057 1104 58-59 1.153468486341957 1104 60-61 1.0836681803653718 1104 62-63 1.144597603691313 1104 64-65 1.3126780773858968 1104 66-67 1.2195860809167627 1104 68-69 1.4830097937618412 1104 70-71 1.3863777358076952 1104 72-73 1.4889075588733753 1104 74-75 1.7284251826075625 1104 76-77 1.7882572999076594 1104 78-79 1.8783509558575204 1104 80-81 1.9289184079195039 1104 82-83 1.858481670978005 1104 84-85 2.155449335292147 1104 86-87 2.2801766257679326 1104 88-89 2.117974491411516 1104 90-91 2.372601182128122 1104 92-93 2.674971612336776 1104 94-95 2.3880924736384763 1104 96-97 2.563223573371868 1104 98-99 2.477932774627231 1104 100-101 2.1624912761368584 1105 1 0.14070157319058652 1105 2 0.14871211234573423 1105 3 0.15771001406204732 1105 4 0.13569778052200832 1105 5 0.07116332664836733 1105 6 0.06072474894280333 1105 7 0.14044945415474785 1105 8 0.1180886747362706 1105 9 0.21095778787212538 1105 10-11 0.25237879399671925 1105 12-13 0.18594649086011827 1105 14-15 0.3388063760911919 1105 16-17 0.15068267767738774 1105 18-19 0.29636993727712024 1105 20-21 0.22416079997753258 1105 22-23 0.2844152089829848 1105 24-25 0.17880394139385913 1105 26-27 0.2939206439110791 1105 28-29 0.31603378077111444 1105 30-31 0.3634208939844612 1105 32-33 0.38454567202658296 1105 34-35 0.43799193701862293 1105 36-37 0.4628681990662713 1105 38-39 0.45602756316908 1105 40-41 0.47082840132176074 1105 42-43 0.3754405651773567 1105 44-45 0.4968384088330353 1105 46-47 0.5294042810378841 1105 48-49 0.44539673242426403 1105 50-51 0.5581863307246806 1105 52-53 0.5409832109344919 1105 54-55 0.5129370116554739 1105 56-57 0.7411663112275377 1105 58-59 0.80186715047288 1105 60-61 0.9287626081799871 1105 62-63 0.9982273231561543 1105 64-65 1.1895852574220243 1105 66-67 1.141917646104048 1105 68-69 1.2412872201517509 1105 70-71 1.2141620556612231 1105 72-73 1.3830693914273233 1105 74-75 1.3301804621749298 1105 76-77 1.4393970036480503 1105 78-79 1.4129819363597527 1105 80-81 1.7071463764208126 1105 82-83 1.9412252736799083 1105 84-85 1.9632723484542183 1105 86-87 2.0224861495847435 1105 88-89 1.9617256934988063 1105 90-91 2.3160403032241383 1105 92-93 2.3649762456977292 1105 94-95 2.2756371504720114 1105 96-97 2.5810077309914377 1105 98-99 2.4250829209285847 1105 100-101 2.065162177619932 1106 1 -0.038700309499237306 1106 2 0.02438356152794796 1106 3 -0.005824140113972476 1106 4 0.06061493338614099 1106 5 0.051024337175732626 1106 6 0.0283522312216391 1106 7 0.0746192543252846 1106 8 0.05869857757014074 1106 9 -0.10784855906065616 1106 10-11 -0.027584447621087804 1106 12-13 -0.05121119930809925 1106 14-15 0.04290268824735932 1106 16-17 0.027103857873463255 1106 18-19 0.060591355622470644 1106 20-21 0.029145824689159383 1106 22-23 0.08954358722136391 1106 24-25 0.013372881007022386 1106 26-27 -0.2301084705358889 1106 28-29 0.04835507389527294 1106 30-31 0.08605556327154318 1106 32-33 -0.06262840465922892 1106 34-35 -0.044756516899710164 1106 36-37 -0.014055452094524412 1106 38-39 0.14959279412935444 1106 40-41 0.13883001052953858 1106 42-43 0.1725121976260553 1106 44-45 0.2328205207715186 1106 46-47 -0.18367998000393015 1106 48-49 -0.02009338446041653 1106 50-51 0.22662692293138065 1106 52-53 0.039357248837518455 1106 54-55 0.035052614279102556 1106 56-57 0.2356664332168279 1106 58-59 0.5266474631758982 1106 60-61 0.5757277317341689 1106 62-63 0.45929534063735744 1106 64-65 0.7158624248237508 1106 66-67 0.502286502857693 1106 68-69 0.3416892147683619 1106 70-71 0.24800300522070629 1106 72-73 0.6934474234015049 1106 74-75 0.6187484843651809 1106 76-77 0.7450841235716013 1106 78-79 0.9153408751510668 1106 80-81 0.943531732114522 1106 82-83 1.3261848119982744 1106 84-85 1.275022499713998 1106 86-87 1.3315982390555838 1106 88-89 1.3981779970739865 1106 90-91 1.4089215415686098 1106 92-93 1.2240300370763109 1106 94-95 1.0779069344731447 1106 96-97 1.2284913957777555 1106 98-99 1.3705048824311241 1106 100-101 1.4141174268325045 1107 1 0.09397677129980053 1107 2 0.07127567916739252 1107 3 0.02020881398171781 1107 4 0.0684059000404389 1107 5 0.01837647238280482 1107 6 0.011910835839330502 1107 7 -0.02856867494821813 1107 8 0.08499659585405794 1107 9 0.10071208250116115 1107 10-11 0.03901815869765102 1107 12-13 0.02217431211984433 1107 14-15 0.10755044617796017 1107 16-17 0.022799274402153458 1107 18-19 -0.035890297571846475 1107 20-21 0.04259246060343713 1107 22-23 0.0016764726076274883 1107 24-25 0.1166434764611779 1107 26-27 0.11408042021326281 1107 28-29 0.12266773954256394 1107 30-31 0.16316352473302942 1107 32-33 0.2562219944629831 1107 34-35 0.2736315886015461 1107 36-37 0.3405644940617023 1107 38-39 0.27308613287045347 1107 40-41 0.34472945707969416 1107 42-43 0.28102018762853476 1107 44-45 0.346440408647851 1107 46-47 0.5184264721699066 1107 48-49 0.5997351661661341 1107 50-51 0.6179084883160542 1107 52-53 0.4193433529330193 1107 54-55 0.5652889463839941 1107 56-57 0.624038044461976 1107 58-59 0.6918322232780696 1107 60-61 0.8380302800677413 1107 62-63 0.923902663919737 1107 64-65 0.9376049708679588 1107 66-67 1.0379613094097948 1107 68-69 1.0715868104327164 1107 70-71 1.0285391270140885 1107 72-73 1.3957498690559795 1107 74-75 1.2182512798511418 1107 76-77 1.250569611735127 1107 78-79 1.3603649177469208 1107 80-81 1.414634981936473 1107 82-83 1.7098984525455663 1107 84-85 1.8995664928109228 1107 86-87 2.2582279868823782 1107 88-89 2.1644789455151034 1107 90-91 2.396094830704296 1107 92-93 2.441819723094092 1107 94-95 2.0183969343371864 1107 96-97 2.205059122577577 1107 98-99 2.256439766580108 1107 100-101 2.0379675754367277 1108 1 0.04437270301196605 1108 2 0.038928034899022634 1108 3 0.06743326270648708 1108 4 0.06218937102919142 1108 5 0.012964812049084173 1108 6 0.11304295841896561 1108 7 0.14551608260724436 1108 8 0.09269325938603146 1108 9 0.06017832388585731 1108 10-11 0.10655683988222364 1108 12-13 0.11490302649571049 1108 14-15 0.19859490831451154 1108 16-17 0.21410758308606148 1108 18-19 0.09639421815425209 1108 20-21 0.12059920138985092 1108 22-23 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-0.4906890943846349 2316 52-53 -0.543130952984157 2316 54-55 -0.40021383542870836 2316 56-57 -0.17362002553555556 2316 58-59 -0.3459113566271057 2316 60-61 -0.18120944915088444 2316 62-63 -0.3200773837684814 2316 64-65 -0.5489162558703313 2316 66-67 -0.3462960973378131 2316 68-69 -0.04838468626473613 2316 70-71 -0.25888516108852144 2316 72-73 -0.04792184963835311 2316 74-75 0.015876461422660526 2316 76-77 0.10029280081617031 2316 78-79 0.11232314944588495 2316 80-81 -0.4252833451389222 2316 82-83 0.10735919198701183 2316 84-85 0.31507064497470694 2316 86-87 0.33603378072676193 2316 88-89 0.5629934849401046 2316 90-91 0.7283556359225933 2316 92-93 0.8321861549739396 2316 94-95 0.8112258871148903 2316 96-97 0.8174566888340529 2316 98-99 0.7763537085320351 2316 100-101 0.4453236200553601 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 20.0 4 50.0 5 88.0 6 211.0 7 591.0 8 1454.0 9 2733.0 10 4507.0 11 7158.0 12 10362.0 13 14212.0 14 18597.0 15 24907.0 16 31293.0 17 38369.0 18 38640.0 19 37494.0 20 38870.0 21 41944.0 22 46647.0 23 51650.0 24 58616.0 25 66298.0 26 77093.0 27 90593.0 28 105468.0 29 123585.0 30 143590.0 31 166433.0 32 195482.0 33 232150.0 34 280889.0 35 336368.0 36 383634.0 37 333979.0 38 110912.0 39 2559.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.93708814320889 19.250272244999323 15.079100247122096 23.73353936466969 2 15.755943491714161 20.45818742952104 39.272135807241746 24.51373327152305 3 19.289927275911744 23.51986383520364 30.30593773848006 26.884271150404555 4 13.339259112890911 16.979571778490897 36.48421852204942 33.196950586568775 5 13.544731079112642 37.39656981479361 34.65249222038204 14.406206885711711 6 32.8912522149499 36.40264549381995 16.720225363534066 13.985876927696081 7 28.80146991838206 32.60685328916051 20.278708381075315 18.312968411382116 8 26.559688206707406 35.183948419381224 19.499719321881667 18.756644052029703 9 26.07482283563073 16.6694574144716 19.889755838436873 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22.594734813420846 26.8549281864712 28.365289221466313 22.18504777864164 36-37 22.83325722862416 26.1323522642394 28.49278512922002 22.54160537791642 38-39 22.048171550957502 26.664705421890055 28.48882347128776 22.79829955586468 40-41 22.679065452132356 26.453864907840817 28.54733092306436 22.319738716962465 42-43 22.291778508930566 26.534681701260983 28.304931880943744 22.868607908864707 44-45 22.304580027054364 26.70019217771579 28.68126503042413 22.31396276480572 46-47 22.89959742738456 27.06692970216042 27.652696915748447 22.38077595470657 48-49 22.379013373742403 27.1270736486079 27.697331025465672 22.796581952184027 50-51 22.599080916081466 27.293251026800093 27.76594475231696 22.341723304801487 52-53 23.1193936586674 26.81465504521 27.73614477464288 22.32980652147972 54-55 22.306806969281322 27.152029927004488 28.009348660380333 22.53181444333385 56-57 22.514964950531702 27.29245784456596 27.594307015571374 22.598270189330965 58-59 22.406006572033828 26.8380395271523 27.65983245291347 23.0961214479004 60-61 21.845308283816397 27.217275518596594 28.136744366873973 22.800671830713032 62-63 22.147301706650175 27.142454799624822 28.192623334697696 22.517620159027306 64-65 21.71116860470991 27.2931052429575 27.898440022345195 23.097286129987392 66-67 21.685861953043403 27.25502913936856 27.85674234433983 23.202366563248212 68-69 22.568604571900575 26.76670155136672 27.886396169900518 22.778297706832188 70-71 22.213792969607695 26.9901318401046 27.426963154667572 23.369112035620134 72-73 22.16400853071341 26.993561867961276 27.589242652989636 23.253186948335678 74-75 22.20922727920109 27.419908951284576 27.33129491648949 23.039568853024843 76-77 22.195979280514987 26.877494825902694 27.804261300574957 23.122264593007365 78-79 22.307336247679192 27.163321199492408 27.61562968732157 22.91371286550683 80-81 22.659576037836075 27.16746197530801 27.21063831698235 22.962323669873562 82-83 22.446512864772135 27.26894790977827 27.428343125387645 22.85619610006196 84-85 22.264429974793508 27.389471978930164 27.374747473937383 22.97135057233894 86-87 22.659798116992572 27.31092174142339 26.96361804067333 23.065662100910707 88-89 22.28502636127196 27.50191502547593 26.676898313109554 23.53616030014255 90-91 22.115475451726816 27.432745605029325 27.01176958929056 23.440009353953304 92-93 22.41467191969816 26.958162439798322 27.22902588599998 23.39813975450354 94-95 21.997715441237087 26.748936871822526 27.432620432526445 23.820727254413942 96-97 22.29470129687663 26.287562132081284 27.527709733415257 23.890026837626824 98-99 22.003963493263086 26.71970570837979 26.96564373725722 24.310687061099898 100-101 22.13101344656259 26.236210288469653 27.476935483508804 24.155840781458952 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 178.0 1 139.0 2 130.5 3 191.0 4 181.0 5 176.0 6 315.5 7 675.0 8 1110.0 9 1324.5 10 1229.0 11 1069.5 12 1106.5 13 1271.0 14 1474.0 15 1648.5 16 1888.5 17 2242.0 18 2630.5 19 3055.0 20 3684.5 21 4608.0 22 5727.5 23 7229.5 24 9382.0 25 12099.0 26 15850.5 27 20811.0 28 25488.5 29 30435.5 30 36088.5 31 44878.5 32 58218.0 33 62696.5 34 61308.5 35 65472.0 36 71960.5 37 78379.0 38 83170.5 39 87329.0 40 92948.0 41 99875.0 42 103680.5 43 105923.0 44 108804.0 45 114414.0 46 117798.5 47 119727.5 48 122069.0 49 118903.5 50 114108.0 51 110041.5 52 107067.5 53 107676.0 54 108708.0 55 101585.5 56 91859.5 57 83509.5 58 76480.5 59 67051.5 60 52790.5 61 43522.5 62 37885.0 63 28352.5 64 19109.0 65 13907.5 66 10551.5 67 8468.5 68 6523.0 69 4875.0 70 3615.5 71 2470.0 72 1802.0 73 1357.5 74 957.5 75 717.0 76 547.5 77 373.0 78 244.0 79 164.0 80 98.0 81 53.0 82 25.5 83 17.5 84 11.0 85 5.0 86 5.5 87 4.0 88 2.0 89 1.5 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02482796848195257 2 0.012413984240976285 3 0.0017001063689192327 4 0.0 5 3.528522652473879E-4 6 0.0 7 0.0 8 6.415495731770689E-5 9 2.5661982927082755E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0017001063689192327 26-27 0.008628841759231577 28-29 2.2454235061197412E-4 30-31 0.0 32-33 3.528522652473879E-4 34-35 0.010152521995527117 36-37 0.001170827971048151 38-39 0.012317751804999725 40-41 0.011948860800422909 42-43 0.00489181549547515 44-45 8.019369664713363E-4 46-47 6.415495731770689E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.2077478658853444E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.1227117530598706E-4 66-67 6.41549573177069E-4 68-69 0.0012670604070247112 70-71 4.8116217988280175E-5 72-73 3.3681352591796124E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.2830991463541377E-4 82-83 0.0013953703216601251 84-85 0.006399456992441263 86-87 0.004859738016816297 88-89 0.0 90-91 0.0017161451082486593 92-93 4.4908470122394825E-4 94-95 3.2077478658853444E-5 96-97 1.6038739329426722E-5 98-99 0.0 100-101 3.2077478658853444E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3117452.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.09985747061907 #Duplication Level Percentage of deduplicated Percentage of total 1 73.1771175693882 36.661631603370836 2 14.484582704088405 14.513510579924466 3 5.222955479796862 7.850079753396348 4 2.3777390635090843 4.764975527365136 5 1.3524821897450396 3.3879582468888634 6 0.7916352852568429 2.379648897604842 7 0.5293797207725407 1.8565293990978287 8 0.372965971811859 1.4948433623332054 9 0.27218911565536064 1.2272972309448678 >10 1.2777669588976854 11.369613105737074 >50 0.08329031249771733 2.8897455912193784 >100 0.04865716708829878 4.604880482404359 >500 0.00481169869430755 1.5817748517657837 >1k 0.004041826902249913 4.287814531720045 >5k 3.849358954523726E-4 1.1296968362270763 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6635 0.21283407090149264 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5887 0.18884011686467025 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 5867 0.18819856729149317 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5798 0.18598522126403227 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5712 0.18322655809937088 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5248 0.1683426080016629 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4978 0.15968168876377248 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4743 0.1521434812789419 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3949 0.12667396322381227 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3918 0.1256795613853878 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3905 0.12526255416282273 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3861 0.12385114510183318 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3727 0.11955276296154681 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3542 0.1136184294096589 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3157 0.10126860012600035 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.623243597656035E-5 2 3.207747865885345E-5 0.0 0.0 0.0 9.623243597656035E-5 3 3.207747865885345E-5 0.0 0.0 0.0 9.623243597656035E-5 4 3.207747865885345E-5 0.0 0.0 0.0 9.623243597656035E-5 5 3.207747865885345E-5 0.0 0.0 3.207747865885345E-5 1.283099146354138E-4 6 3.207747865885345E-5 0.0 0.0 6.41549573177069E-5 1.6038739329426725E-4 7 6.41549573177069E-5 0.0 0.0 6.41549573177069E-5 1.6038739329426725E-4 8 6.41549573177069E-5 0.0 0.0 6.41549573177069E-5 1.6038739329426725E-4 9 6.41549573177069E-5 0.0 0.0 6.41549573177069E-5 1.6038739329426725E-4 10-11 6.41549573177069E-5 0.0 0.0 9.623243597656035E-5 1.924648719531207E-4 12-13 6.41549573177069E-5 0.0 0.0 9.623243597656035E-5 4.65123440553375E-4 14-15 6.41549573177069E-5 0.0 0.0 1.4434865396484054E-4 5.77394615859362E-4 16-17 6.41549573177069E-5 0.0 0.0 1.924648719531207E-4 6.736270518359224E-4 18-19 6.41549573177069E-5 0.0 0.0 2.2454235061197412E-4 7.377820091536293E-4 20-21 6.41549573177069E-5 0.0 0.0 2.726585686002543E-4 8.821306631184698E-4 22-23 6.41549573177069E-5 0.0 0.0 3.528522652473879E-4 9.623243597656035E-4 24-25 1.283099146354138E-4 0.0 0.0 5.613558765299354E-4 9.623243597656035E-4 26-27 1.283099146354138E-4 0.0 0.0 9.302468811067501E-4 9.783630990950302E-4 28-29 1.7642613262369396E-4 0.0 0.0 0.0030794379512499312 9.944018384244568E-4 30-31 1.924648719531207E-4 0.0 0.0 0.011676202231822655 9.944018384244568E-4 32-33 1.924648719531207E-4 0.0 0.0 0.03291149310398364 0.0011227117530598707 34-35 2.085036112825474E-4 0.0 0.0 0.06263127708141136 0.0011868667103775777 36-37 2.2454235061197412E-4 0.0 0.0 0.11406751411088287 0.0011868667103775777 38-39 3.528522652473879E-4 0.0 0.0 0.1899467898784007 0.0012349829283658576 40-41 3.528522652473879E-4 0.0 0.0 0.2741020551399027 0.0012510216676952844 42-43 4.4908470122394825E-4 0.0 0.0 0.35833751409805187 0.0012670604070247112 44-45 4.8116217988280173E-4 0.0 0.0 0.46991902361287363 0.0013151766250129914 46-47 4.8116217988280173E-4 0.0 0.0 0.6032971798763862 0.001395370321660125 48-49 4.8116217988280173E-4 0.0 0.0 0.7398670452664549 0.0015397189756249656 50-51 4.8116217988280173E-4 0.0 0.0 0.8955871654158588 0.001635951411601526 52-53 4.8116217988280173E-4 0.0 0.0 1.041764235664254 0.0016680288902603793 54-55 4.8116217988280173E-4 0.0 0.0 1.2016544280393089 0.0016680288902603793 56-57 5.132396585416552E-4 0.0 0.0 1.3902860412926967 0.0016680288902603793 58-59 5.132396585416552E-4 0.0 0.0 1.5879635035278812 0.0016680288902603793 60-61 5.132396585416552E-4 0.0 0.0 1.8090254477053695 0.0017001063689192327 62-63 5.132396585416552E-4 0.0 0.0 2.0213783564269794 0.001796338804895793 64-65 5.132396585416552E-4 0.0 0.0 2.245407467380412 0.0019567261981900605 66-67 5.77394615859362E-4 0.0 0.0 2.5059086715689607 0.0019888036768489136 68-69 5.77394615859362E-4 0.0 0.0 2.7988883228995984 0.0020208811555077672 70-71 5.77394615859362E-4 0.0 0.0 3.102918665628212 0.002036919894837194 72-73 5.77394615859362E-4 0.0 0.0 3.427879563181727 0.002101074852154901 74-75 5.934333551887887E-4 0.0 0.0 3.7482694200263547 0.0021652298094726077 76-77 6.094720945182155E-4 0.0 0.0 4.099918779824035 0.0021812685488020343 78-79 6.094720945182155E-4 0.0 0.0 4.494503844806592 0.0021973072881314613 80-81 6.736270518359224E-4 0.0 0.0 4.904229479716127 0.002261462245449168 82-83 6.736270518359224E-4 0.0 0.0 5.332367587375844 0.002341655942096302 84-85 6.736270518359224E-4 0.0 0.0 5.768476948482286 0.002341655942096302 86-87 6.736270518359224E-4 0.0 0.0 6.224217726527947 0.002405810899414009 88-89 6.736270518359224E-4 0.0 0.0 6.698723829589037 0.0024699658567317157 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6675 0.0 37.586205 1 GTATCAA 10345 0.0 32.1524 1 TAAGGTG 2290 0.0 25.719225 5 ATCAACG 12700 0.0 25.619608 3 TATCAAC 12785 0.0 25.60076 2 TCAACGC 12865 0.0 25.327133 4 CAACGCA 12940 0.0 25.180336 5 AACGCAG 13465 0.0 24.16328 6 CGTAACC 120 1.7119452E-4 23.749563 3 GTAAGGT 2525 0.0 22.761227 4 GTACATG 9435 0.0 22.41114 1 TAAGGTA 1345 0.0 22.247948 4 AAGGTAA 2305 0.0 21.851477 1 GGTAAGG 2605 0.0 21.698257 3 TACATGG 9235 0.0 21.604626 2 AGGTAAG 2510 0.0 21.575731 2 ACGCAGA 15240 0.0 21.255487 7 GTGTAAG 1545 0.0 21.22103 1 CGCAGAG 15450 0.0 20.905094 8 ACATGGG 9175 0.0 20.863377 3 >>END_MODULE