FastQCFastQC Report
Thu 26 May 2016
SRR938471_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938471_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2885358
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55990.19404871076656693No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44020.15256339074735267No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA41410.1435177194649676No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA38550.13360560457315868No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA35470.12293101930505676No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA33630.11655399433969718No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC31160.10799353147858948No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA29880.10355734019833933No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44450.046.7549061
GTATCAA74300.038.9803731
ATCAACG90800.031.2689693
TATCAAC92350.030.8471472
TCAACGC92050.030.7410144
CAACGCA93600.030.2319495
AACGCAG97850.028.993926
ACGCAGA111200.025.2156857
CGCAGAG111600.025.0392678
TACATGG88200.024.1026042
TAAGGTG19000.024.0293875
GTACATG91050.023.9223061
GTAAGGT18700.023.1432764
ACATGGG90900.022.2879853
GCAGAGT125950.021.5061459
GAGTACT67500.021.04786912-13
AGAGTAC106250.020.14874510-11
GTACTTT72500.019.49392314-15
GGTAAGG19900.019.3578433
CAGAGTA118350.019.35356110-11
Produced by FastQC (version 0.11.5)