##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938471_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2885358 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.97581859859331 31.0 31.0 34.0 27.0 34.0 2 31.026466733070905 31.0 31.0 34.0 27.0 34.0 3 31.07079676074858 31.0 31.0 34.0 27.0 34.0 4 34.71317077464911 37.0 35.0 37.0 32.0 37.0 5 34.68301368495695 37.0 35.0 37.0 32.0 37.0 6 34.69230473306952 37.0 35.0 37.0 31.0 37.0 7 34.5956023481315 37.0 35.0 37.0 30.0 37.0 8 34.55316636618402 37.0 35.0 37.0 30.0 37.0 9 36.023642126904186 39.0 35.0 39.0 30.0 39.0 10-11 35.85194072971188 38.0 35.0 39.0 30.0 39.0 12-13 35.713797213378726 38.0 35.0 39.0 30.0 39.0 14-15 36.67236907863773 39.0 36.0 41.0 30.0 41.0 16-17 36.492835377793675 39.0 36.0 40.5 30.0 41.0 18-19 36.623364587687206 39.0 36.0 41.0 30.0 41.0 20-21 36.59010961551392 39.0 36.0 41.0 30.0 41.0 22-23 36.497732343785415 39.0 36.0 41.0 29.5 41.0 24-25 36.35676248839832 39.0 36.0 41.0 29.0 41.0 26-27 36.14860201056507 39.0 35.0 41.0 28.0 41.0 28-29 35.93824371187215 39.0 35.0 40.0 27.0 41.0 30-31 35.6779796475862 38.0 34.5 40.0 27.0 41.0 32-33 35.41102906467759 38.0 34.0 40.0 26.5 41.0 34-35 35.166410719224444 38.0 34.0 40.0 25.5 41.0 36-37 34.891995204754494 38.0 34.0 40.0 25.0 41.0 38-39 34.752787175802794 38.0 33.5 40.0 24.5 41.0 40-41 34.64674608835368 38.0 33.0 40.0 24.0 41.0 42-43 34.35414877460613 38.0 33.0 40.0 23.5 41.0 44-45 34.02456402290461 38.0 33.0 40.0 23.0 41.0 46-47 33.666272088246934 38.0 33.0 40.0 20.5 41.0 48-49 33.31468902645703 37.5 32.0 40.0 18.5 41.0 50-51 32.62581350390489 36.5 31.0 39.5 17.5 40.5 52-53 32.64667538655515 37.0 31.0 39.0 16.5 40.0 54-55 32.74809087815099 37.0 31.0 40.0 14.5 41.0 56-57 32.42185146522546 37.0 31.0 40.0 11.5 41.0 58-59 32.04370740129994 36.0 31.0 39.0 8.5 41.0 60-61 31.568231221221076 36.0 30.0 39.0 7.5 40.5 62-63 30.996459018257006 35.0 29.0 39.0 4.0 40.0 64-65 30.47203310646374 35.0 28.5 38.0 2.0 40.0 66-67 29.929961204120943 34.5 28.0 38.0 2.0 40.0 68-69 29.349645347301788 34.0 27.5 37.5 2.0 39.5 70-71 28.70608222619169 34.0 26.0 37.0 2.0 39.0 72-73 28.06859859331147 33.5 26.0 36.0 2.0 39.0 74-75 27.39328741875358 33.0 25.0 35.5 2.0 38.5 76-77 26.6444713273015 32.5 23.0 35.0 2.0 37.5 78-79 25.872989590893056 32.0 20.5 35.0 2.0 37.0 80-81 25.195015835123407 31.0 18.5 35.0 2.0 36.0 82-83 24.46899344899316 31.0 14.5 34.0 2.0 36.0 84-85 23.769244752297634 30.5 7.5 34.0 2.0 35.0 86-87 23.023741421341825 30.0 2.0 34.0 2.0 35.0 88-89 22.207100470721485 29.0 2.0 34.0 2.0 35.0 90-91 21.310583816635578 29.0 2.0 34.0 2.0 35.0 92-93 20.382967728787904 27.5 2.0 34.0 2.0 35.0 94-95 19.385936857748675 25.5 2.0 33.0 2.0 35.0 96-97 18.163833569352573 24.5 2.0 32.5 2.0 34.0 98-99 16.678588410866176 11.0 2.0 32.0 2.0 34.0 100-101 13.235647188321172 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5592266447175156 1101 2 0.5123424670040997 1101 3 0.5919384495914208 1101 4 0.4654865028670443 1101 5 0.4513172120646658 1101 6 0.6397430622939524 1101 7 0.44714494554961703 1101 8 0.5325516163723876 1101 9 0.7681712860834793 1101 10-11 0.782280248429565 1101 12-13 0.8424055467742164 1101 14-15 0.9655867478326883 1101 16-17 0.751318841526448 1101 18-19 0.9047243361675683 1101 20-21 0.9687573629797441 1101 22-23 0.8325559750527916 1101 24-25 0.9494450482987489 1101 26-27 0.967371895529034 1101 28-29 0.9853044590667324 1101 30-31 0.9233724291121561 1101 32-33 0.9407536518599287 1101 34-35 0.8084591010733106 1101 36-37 0.8690655836623904 1101 38-39 1.0312842592837015 1101 40-41 0.9904090323576611 1101 42-43 1.2274739985164516 1101 44-45 1.2473900893920629 1101 46-47 1.2339647549996968 1101 48-49 0.9968077491748275 1101 50-51 1.2061277566291722 1101 52-53 1.0997130427162531 1101 54-55 1.1226962676939465 1101 56-57 1.0958675279393901 1101 58-59 1.1874832633398142 1101 60-61 1.281992712895729 1101 62-63 1.1067965211773974 1101 64-65 0.9700297355653866 1101 66-67 1.0771671647921153 1101 68-69 1.0843304587220288 1101 70-71 1.1390313042576956 1101 72-73 1.3245123994840853 1101 74-75 1.1053993415316263 1101 76-77 1.0782254041621862 1101 78-79 1.355511324901446 1101 80-81 1.296434140665589 1101 82-83 1.2661064817425078 1101 84-85 1.1660491551547665 1101 86-87 1.3009688883892778 1101 88-89 1.2945210001936402 1101 90-91 1.2009070961688977 1101 92-93 1.1521219147611603 1101 94-95 1.0261304309977994 1101 96-97 0.8489694934676137 1101 98-99 0.9886186648973165 1101 100-101 1.1131270684182564 1102 1 0.5254013076744428 1102 2 0.4080310157066407 1102 3 0.5070208737589432 1102 4 0.35160262025573275 1102 5 0.39337187235040716 1102 6 0.37018325480265446 1102 7 0.42254882718481923 1102 8 0.5234092504329411 1102 9 0.6672522083021306 1102 10-11 0.7206258900554872 1102 12-13 0.6725674431657325 1102 14-15 0.8896219575656517 1102 16-17 0.9418349160398023 1102 18-19 0.8604838207293639 1102 20-21 0.8091176339130541 1102 22-23 0.8527580270602613 1102 24-25 0.8416572570911498 1102 26-27 0.6581178280460236 1102 28-29 0.5432224441041811 1102 30-31 0.786815914546672 1102 32-33 0.7530234904620912 1102 34-35 0.6493871291474704 1102 36-37 0.9593852817717163 1102 38-39 0.8229690804031407 1102 40-41 0.880592862345658 1102 42-43 1.1043973045305364 1102 44-45 1.1456880905851676 1102 46-47 1.1108097361118539 1102 48-49 1.2155970797225777 1102 50-51 1.0068097080658944 1102 52-53 0.9965318578008748 1102 54-55 1.0015191821753504 1102 56-57 1.2083529108989666 1102 58-59 1.2601101270493729 1102 60-61 1.091413860383085 1102 62-63 1.309478604598798 1102 64-65 1.1852370466242697 1102 66-67 1.0269849117979568 1102 68-69 1.1103273070959112 1102 70-71 1.2793779175322335 1102 72-73 1.2795931994266745 1102 74-75 1.3117092979648781 1102 76-77 1.5073591451786044 1102 78-79 1.4374265117588187 1102 80-81 1.3409028741388056 1102 82-83 1.2789593095141818 1102 84-85 1.3483559402822713 1102 86-87 1.6156101216351964 1102 88-89 1.3217532041396183 1102 90-91 1.4972441147085505 1102 92-93 1.4801629381173314 1102 94-95 1.589096370686054 1102 96-97 1.9139327181549923 1102 98-99 1.9283799930462493 1102 100-101 1.7518777954163536 1103 1 0.3843658038569835 1103 2 0.29547404412711487 1103 3 0.2124959514144713 1103 4 0.2810401136030052 1103 5 0.21253125729926836 1103 6 0.34977992988805084 1103 7 0.2990305720876165 1103 8 0.3937970926252916 1103 9 0.42821107264445857 1103 10-11 0.6004581928201205 1103 12-13 0.6631725756881508 1103 14-15 0.5945369961273101 1103 16-17 0.5972983816536441 1103 18-19 0.7389432414820121 1103 20-21 0.6104101993033666 1103 22-23 0.575450375692462 1103 24-25 0.5403042123170607 1103 26-27 0.5095490121839745 1103 28-29 0.5346742879996285 1103 30-31 0.6430370658806055 1103 32-33 0.6702671304500853 1103 34-35 0.6237934635162148 1103 36-37 0.6415213652235963 1103 38-39 0.6365256552379108 1103 40-41 0.7580289719246238 1103 42-43 0.8071991934226475 1103 44-45 0.8564708389619113 1103 46-47 0.6986741848785556 1103 48-49 0.9275011158747404 1103 50-51 1.0777871032914135 1103 52-53 1.1823257343842215 1103 54-55 1.1494529645076383 1103 56-57 1.0829138305726644 1103 58-59 0.8913044255369016 1103 60-61 1.069597737063205 1103 62-63 1.1019181977902726 1103 64-65 1.204694473976435 1103 66-67 1.1642537595931195 1103 68-69 1.281493639916377 1103 70-71 1.3384251495117958 1103 72-73 1.4213205873946642 1103 74-75 1.4760378316543132 1103 76-77 1.4736310024279042 1103 78-79 1.3578283851682116 1103 80-81 1.524617469507536 1103 82-83 1.4250182309490462 1103 84-85 1.615452252491366 1103 86-87 1.7012501386661896 1103 88-89 1.508611517877629 1103 90-91 1.6691906315798768 1103 92-93 1.67848572384613 1103 94-95 1.6141638656783606 1103 96-97 1.8875080657904917 1103 98-99 1.7454117132734588 1103 100-101 1.5731563779731292 1104 1 0.4931823320083204 1104 2 0.42033910989269785 1104 3 0.416815659922225 1104 4 0.33877751088026287 1104 5 0.4798954496367216 1104 6 0.5403673390968535 1104 7 0.3688354876872637 1104 8 0.4998105657136591 1104 9 0.6612837168752463 1104 10-11 0.7305562323720807 1104 12-13 0.7847105907596301 1104 14-15 0.9370197443903763 1104 16-17 0.9534555477578763 1104 18-19 0.8778173962902329 1104 20-21 1.0278716692400494 1104 22-23 1.0333279869730845 1104 24-25 0.9484066486548031 1104 26-27 0.9853871479428804 1104 28-29 1.0103867995575513 1104 30-31 1.1280205944714723 1104 32-33 1.0540605795185698 1104 34-35 0.9542236385105412 1104 36-37 1.1402115292712836 1104 38-39 1.24553230407178 1104 40-41 1.2148122255265008 1104 42-43 1.3858335732200757 1104 44-45 1.2765929572003074 1104 46-47 1.209840344965066 1104 48-49 0.9940233259394731 1104 50-51 1.0551848533993322 1104 52-53 1.0608620770796975 1104 54-55 1.1372023692779933 1104 56-57 1.4391700995326744 1104 58-59 1.2551791193470478 1104 60-61 1.2569383946161707 1104 62-63 1.3460363391744004 1104 64-65 1.359459202081812 1104 66-67 1.4839894624442955 1104 68-69 1.5180574241803626 1104 70-71 1.5119048823040337 1104 72-73 1.6297412431248155 1104 74-75 1.5493147159683254 1104 76-77 1.6280729418744677 1104 78-79 1.7066798700450398 1104 80-81 1.7124531513376304 1104 82-83 1.7606016319434552 1104 84-85 1.8150642690985066 1104 86-87 1.9493605081324183 1104 88-89 2.03452789466742 1104 90-91 1.8593286479253557 1104 92-93 1.934841475413279 1104 94-95 1.9276187259813078 1104 96-97 2.020489030692154 1104 98-99 2.122892959681135 1104 100-101 1.8388276142328692 1105 1 0.45385574630919834 1105 2 0.4386823791146739 1105 3 0.34415333342117904 1105 4 0.47895104090378027 1105 5 0.3914559184083686 1105 6 0.5701536660978377 1105 7 0.46456929701152916 1105 8 0.4110151903044965 1105 9 0.7783440241979918 1105 10-11 0.751336418686698 1105 12-13 0.7446866208142211 1105 14-15 0.8328622180924938 1105 16-17 0.7147680511726904 1105 18-19 0.7021241098212485 1105 20-21 0.820941477778284 1105 22-23 0.8129991189468413 1105 24-25 0.7925423834536929 1105 26-27 0.7899843147431724 1105 28-29 0.7614083713359534 1105 30-31 0.9463786719702298 1105 32-33 0.9414155539622726 1105 34-35 0.9061754702228626 1105 36-37 0.9365298962009234 1105 38-39 0.8408125439682053 1105 40-41 1.0312198431010557 1105 42-43 1.124630226619864 1105 44-45 1.108239733721021 1105 46-47 1.2273560859405208 1105 48-49 1.0682313762486828 1105 50-51 1.2321030176795205 1105 52-53 1.079430987858828 1105 54-55 1.1191434236601268 1105 56-57 1.061465830317026 1105 58-59 1.0772998240381213 1105 60-61 0.9936512198032261 1105 62-63 1.0978115150622578 1105 64-65 1.1471350694212212 1105 66-67 1.29296427477313 1105 68-69 1.198847255220624 1105 70-71 1.477963033926251 1105 72-73 1.4268008446215426 1105 74-75 1.4570429715439452 1105 76-77 1.5930007752676367 1105 78-79 1.6415872344836515 1105 80-81 1.76204129833798 1105 82-83 1.5867602594237233 1105 84-85 1.6413470609898084 1105 86-87 1.7409882976987099 1105 88-89 2.00864383585359 1105 90-91 1.5749997090820145 1105 92-93 1.6191338215156534 1105 94-95 1.5777123358639962 1105 96-97 1.873207058825301 1105 98-99 1.9698423843156796 1105 100-101 1.7929391914913886 1106 1 0.34033720868259465 1106 2 0.281590441083047 1106 3 0.1970882162176899 1106 4 0.15944478051760314 1106 5 0.36437516899486866 1106 6 0.035058639019631244 1106 7 0.4623125294210837 1106 8 0.2568563691744217 1106 9 0.16123494890600654 1106 10-11 0.1285303700463416 1106 12-13 0.21532475061834333 1106 14-15 0.5047455462204695 1106 16-17 0.259926140321987 1106 18-19 0.18902607448903552 1106 20-21 0.37311992273349404 1106 22-23 0.3940458491441703 1106 24-25 0.05849003078531467 1106 26-27 -0.04531383642251541 1106 28-29 0.30849815335988495 1106 30-31 0.08579763712808841 1106 32-33 0.21492140484549083 1106 34-35 0.006453315904877854 1106 36-37 0.05361883105693721 1106 38-39 0.17254324106274765 1106 40-41 0.43727850174570904 1106 42-43 -0.15335268736534857 1106 44-45 0.12418195640798757 1106 46-47 0.3724447858055484 1106 48-49 0.48889877605030563 1106 50-51 0.09440043317106017 1106 52-53 0.08540357932237441 1106 54-55 0.3012712509098989 1106 56-57 -0.44435919927037304 1106 58-59 -0.20134397325006148 1106 60-61 0.4329279784787943 1106 62-63 0.3656450343339408 1106 64-65 0.39216586409011356 1106 66-67 0.03845177835397351 1106 68-69 0.1502851438473627 1106 70-71 0.0893289686813965 1106 72-73 0.3889672376322011 1106 74-75 0.6341973229976681 1106 76-77 0.6898814660949775 1106 78-79 0.8030550140565644 1106 80-81 0.816950936454166 1106 82-83 0.4145442389938161 1106 84-85 0.605457785142935 1106 86-87 0.34741711331873404 1106 88-89 0.4263829663084451 1106 90-91 0.5174404783104762 1106 92-93 0.4834317189926409 1106 94-95 0.28225566042325667 1106 96-97 0.33573038191182647 1106 98-99 0.6724402235527585 1106 100-101 0.21175569099820812 1107 1 0.31332988555257657 1107 2 0.1370161576545712 1107 3 0.18315515158732865 1107 4 0.16544519720861928 1107 5 0.2381957138431403 1107 6 0.39944998122366115 1107 7 0.33976640862625374 1107 8 0.2639893137502227 1107 9 0.4147897822411508 1107 10-11 0.4785303535371881 1107 12-13 0.4526885624892856 1107 14-15 0.5714182552370843 1107 16-17 0.5590477213177039 1107 18-19 0.5581321005609468 1107 20-21 0.6993462308609892 1107 22-23 0.6229921392568727 1107 24-25 0.6653547676640841 1107 26-27 0.5474250089207331 1107 28-29 0.7960662659972044 1107 30-31 0.687665548716943 1107 32-33 0.8490409370553351 1107 34-35 0.6847706568218541 1107 36-37 0.7331344062870002 1107 38-39 0.7827339118725476 1107 40-41 0.7326384722669701 1107 42-43 0.8488980054412494 1107 44-45 0.8593520320496282 1107 46-47 0.8210806840338876 1107 48-49 0.9350363441794514 1107 50-51 0.8990605047613514 1107 52-53 1.0592983163035044 1107 54-55 1.068586628228239 1107 56-57 0.9914190405435619 1107 58-59 0.9731559562558516 1107 60-61 1.0295334325085648 1107 62-63 1.0593284343477443 1107 64-65 1.1012881285647644 1107 66-67 1.17555253338357 1107 68-69 1.295171285172973 1107 70-71 1.5560012814783342 1107 72-73 1.5027739081884235 1107 74-75 1.3387587689088924 1107 76-77 1.5527914420147262 1107 78-79 1.7500553505130974 1107 80-81 1.6397014075428409 1107 82-83 1.4427337825586655 1107 84-85 1.6610201644816662 1107 86-87 1.6574639563890017 1107 88-89 1.519473126424117 1107 90-91 1.3076682484885396 1107 92-93 1.3966698473433787 1107 94-95 1.5183411685898385 1107 96-97 1.578093990720184 1107 98-99 1.5996682080196258 1107 100-101 1.3619648784738363 1108 1 0.3044815598329116 1108 2 0.23143565381221265 1108 3 0.31932241652844695 1108 4 0.25952559043883383 1108 5 0.23257435617190225 1108 6 0.3180917609650038 1108 7 0.2739933589121435 1108 8 0.3435854906069338 1108 9 0.447669847994824 1108 10-11 0.46557016260609174 1108 12-13 0.3454769735660008 1108 14-15 0.4929575887544999 1108 16-17 0.5531423245753544 1108 18-19 0.6006704635980853 1108 20-21 0.49079873788122086 1108 22-23 0.4508258690176845 1108 24-25 0.6965083150843938 1108 26-27 0.6951054641583383 1108 28-29 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-2.5745947191610234 2316 88-89 -2.7229767473594073 2316 90-91 -2.808814386709411 2316 92-93 -3.176535090211786 2316 94-95 -3.5242022941777726 2316 96-97 -3.62785803926797 2316 98-99 -3.4549184427006434 2316 100-101 -3.1837694137513335 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12234.0 3 4494.0 4 4744.0 5 5321.0 6 6192.0 7 7684.0 8 9425.0 9 11947.0 10 14609.0 11 17621.0 12 20970.0 13 23718.0 14 26860.0 15 30174.0 16 33992.0 17 37723.0 18 37273.0 19 37118.0 20 39533.0 21 42473.0 22 46941.0 23 52161.0 24 57967.0 25 63776.0 26 72053.0 27 80949.0 28 91273.0 29 104752.0 30 120034.0 31 137932.0 32 160756.0 33 191361.0 34 231121.0 35 278850.0 36 321709.0 37 305404.0 38 137229.0 39 6985.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.51802044109736 18.116345391325567 14.396769962788996 23.968864204788073 2 15.563493476021616 19.32449962131817 40.17579995885326 24.936206943806948 3 19.549223585351484 22.935233895910404 29.273472690342505 28.242069828395604 4 13.10977577392382 15.985727976594047 36.377837350563944 34.526658898918186 5 14.267704159679603 36.794092440641336 34.03719438137622 14.90100901830284 6 33.42635198516882 35.26418731023917 17.013567031710526 14.29589367288148 7 29.55400547808057 31.866502899721866 20.03943718806193 18.540054434135634 8 26.785703142820893 34.791094520360836 19.40504179773598 19.018160539082288 9 26.393964027517548 16.718797936010787 19.686103085064158 37.20113495140751 10-11 25.325368956650358 26.102457433947045 27.152359101566436 21.41981450783616 12-13 26.24381644005534 23.202695235431715 27.316428178463813 23.237060146049135 14-15 22.781086422714818 24.910288708979035 25.37337958926425 26.935245279041897 16-17 22.841002499974866 27.920188016415032 26.31474028308549 22.92406920052461 18-19 22.924370091693945 26.532459351052207 28.164536664229306 22.378633893024542 20-21 23.17459877099777 25.904672566617194 28.405678240002217 22.51505042238282 22-23 22.928927924604707 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22.909347912666806 48-49 23.055753410176024 26.6905825244401 27.001609987816778 23.2520540775671 50-51 23.165634071314066 26.807365365452103 27.083627225856766 22.943373337377064 52-53 23.421988430534945 26.436534401212853 27.1892133862513 22.9522637820009 54-55 22.879333532058258 26.687226608805364 27.381510263218097 23.05192959591828 56-57 22.96554467251364 26.825033557570404 27.130773570239526 23.078648199676426 58-59 22.938539439669928 26.331769687337243 27.18700576379615 23.542685109196682 60-61 22.558481118500033 26.598816701789367 27.51539268729055 23.32730949242005 62-63 22.78336663424096 26.624175297780127 27.597379808679655 22.995078259299255 64-65 22.46632660966007 26.683054287981594 27.382682325718182 23.46793677664015 66-67 22.40998575078395 26.721300792944092 27.370938184883492 23.49777527138847 68-69 23.035638494502937 26.215836484791115 27.500324518841246 23.248200501864705 70-71 22.73146918701763 26.33425042971559 27.132665110283842 23.801615272982936 72-73 22.718352124326323 26.457720439113647 27.22777443042872 23.596153006131313 74-75 22.73803322762457 26.787820062246542 27.070555616706205 23.403591093422683 76-77 22.91539919901244 26.345162338123796 27.163656116829234 23.57578234603453 78-79 22.902993197585424 26.559622462784972 27.1508414160349 23.386542923594707 80-81 23.101955059419833 26.53199118823027 26.865118645571656 23.500935106778243 82-83 22.969090152249567 26.63078119102439 26.99536634760122 23.40476230912482 84-85 22.864715923368195 26.67057161919781 26.985035829094784 23.47967662833921 86-87 23.066167187030167 26.654968236595984 26.72496817593746 23.553896400436393 88-89 22.892634176725185 26.666028718419177 26.589873646375235 23.851463458480403 90-91 22.81306295998902 26.686591723214026 26.718349774018584 23.781995542778372 92-93 23.036577428130897 26.235349484615604 26.968510113113286 23.75956297414021 94-95 22.718798766352684 25.992135489690682 27.184173474848976 24.104892269107655 96-97 23.040929873410743 25.62030365541454 27.147023594778457 24.19174287639626 98-99 22.809809430176397 25.887693041559835 26.73827537290977 24.564222155353995 100-101 23.406568060779794 25.2485870709404 27.1048264165275 24.24001845175231 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 186.0 1 132.5 2 120.0 3 166.0 4 149.0 5 166.0 6 277.0 7 568.5 8 951.5 9 1142.5 10 1079.0 11 993.0 12 1043.5 13 1172.5 14 1331.5 15 1547.0 16 1748.5 17 1952.5 18 2223.0 19 2569.5 20 3047.5 21 3748.5 22 4564.0 23 5647.5 24 7164.0 25 9146.0 26 12122.5 27 16054.0 28 19683.5 29 23451.0 30 27949.0 31 34509.5 32 43987.5 33 48348.5 34 49168.5 35 53431.5 36 59610.0 37 66194.5 38 71614.0 39 76125.0 40 81322.5 41 86978.0 42 90731.5 43 94014.0 44 97649.0 45 103012.0 46 106871.0 47 109264.5 48 111028.0 49 110065.5 50 108382.5 51 106900.0 52 106533.0 53 106559.5 54 106436.0 55 101180.0 56 93378.0 57 86370.0 58 79506.5 59 70203.0 60 57776.5 61 48349.0 62 41013.5 63 31889.5 64 23236.5 65 17443.0 66 13106.0 67 10020.5 68 7726.5 69 5919.5 70 4545.0 71 3302.0 72 2448.0 73 1990.5 74 1551.0 75 1104.5 76 786.0 77 585.5 78 425.5 79 271.5 80 174.0 81 110.0 82 65.0 83 35.5 84 27.0 85 18.5 86 11.5 87 10.0 88 9.5 89 6.5 90 6.5 91 5.5 92 2.0 93 0.5 94 1.0 95 1.5 96 1.5 97 2.0 98 1.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00658497143162131 2 0.1036613134314702 3 0.11312287764637871 4 0.1163113901290585 5 0.15384572728929996 6 0.09641784485668675 7 0.02672112091463174 8 0.026062623771469604 9 0.022631507078151133 10-11 0.05255846934765114 12-13 0.055539035364069206 14-15 0.028661954599741177 16-17 0.032197044526190516 18-19 0.03774228362650319 20-21 0.05954200483960743 22-23 0.032370333248075284 24-25 0.049993796263756524 26-27 0.058831521079879864 28-29 0.05484588047653012 30-31 0.06872630709950031 32-33 0.05203860318199683 34-35 0.06321572574356457 36-37 0.05411806784461408 38-39 0.032595608586525486 40-41 0.037794270243068626 42-43 0.03320211911312219 44-45 0.006810246770071513 46-47 0.01251144572008049 48-49 0.008421831883599885 50-51 0.008075254439830343 52-53 0.009132315643327449 54-55 0.01032800782433237 56-57 0.013533849179200641 58-59 0.018732510835743777 60-61 0.02457234076326057 62-63 0.01044930992965171 64-65 0.008785738199557906 66-67 0.009946772636185874 68-69 0.011003833839682979 70-71 0.030914707984243204 72-73 0.028419350389102496 74-75 0.02982298903636914 76-77 0.023186030988182404 78-79 0.00459215112994644 80-81 0.004366875791496238 82-83 0.005579896844689636 84-85 0.004609480002134917 86-87 0.012320828126007242 88-89 0.03852208287498467 90-91 0.03639063159580198 92-93 0.02982298903636914 94-95 0.04815693581177795 96-97 0.043894033253412575 98-99 0.02585467730520788 100-101 0.07496470108735206 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2885358.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.27267307936828 #Duplication Level Percentage of deduplicated Percentage of total 1 76.3915038121392 42.22362616249687 2 12.999992902443752 14.370887154617634 3 4.575077030872725 7.58630211121065 4 2.0860030681580364 4.611958625154334 5 1.1231380521398715 3.1039421189462804 6 0.6713147505597248 2.22632164446272 7 0.42217498844627344 1.6334318083073867 8 0.2967903575865429 1.3123517126391835 9 0.19573268186573686 0.9736801682141585 >10 1.1220477571164538 11.154143342213045 >50 0.07398783227332713 2.817764802151559 >100 0.037140603865057145 3.8156005905849657 >500 0.0022649611260512504 0.9035621151359389 >1k 0.002768285820541163 3.0717217098932514 >5k 6.291558683048098E-5 0.19470593397213112 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5599 0.19404871076656693 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4402 0.15256339074735267 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4141 0.1435177194649676 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3855 0.13360560457315868 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3547 0.12293101930505676 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3363 0.11655399433969718 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3116 0.10799353147858948 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2988 0.10355734019833933 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.4657744376954265E-5 2 0.0 0.0 0.0 0.0 3.4657744376954265E-5 3 0.0 0.0 0.0 0.0 3.4657744376954265E-5 4 0.0 0.0 0.0 0.0 3.4657744376954265E-5 5 0.0 0.0 0.0 0.0 3.4657744376954265E-5 6 0.0 0.0 0.0 0.0 3.4657744376954265E-5 7 0.0 0.0 0.0 0.0 3.4657744376954265E-5 8 0.0 0.0 0.0 0.0 3.4657744376954265E-5 9 0.0 0.0 0.0 0.0 3.4657744376954265E-5 10-11 0.0 0.0 0.0 0.0 6.931548875390853E-5 12-13 0.0 0.0 0.0 1.7328872188477133E-5 3.639063159580198E-4 14-15 0.0 0.0 0.0 3.4657744376954265E-5 4.678795490888826E-4 16-17 0.0 0.0 0.0 6.931548875390853E-5 6.238393987851767E-4 18-19 0.0 0.0 0.0 6.931548875390853E-5 6.238393987851767E-4 20-21 0.0 0.0 0.0 2.079464662617256E-4 7.104837597275624E-4 22-23 0.0 0.0 0.0 4.158929325234512E-4 7.624703762929938E-4 24-25 0.0 0.0 0.0 6.238393987851767E-4 7.624703762929938E-4 26-27 0.0 0.0 0.0 9.877457147431964E-4 8.317858650469023E-4 28-29 0.0 0.0 0.0 0.0029632371442295897 8.317858650469023E-4 30-31 0.0 0.0 0.0 0.011038491584059933 8.317858650469023E-4 32-33 0.0 0.0 0.0 0.030169566480138688 9.011013538008109E-4 34-35 0.0 0.0 0.0 0.05879686333550291 9.704168425547193E-4 36-37 0.0 0.0 0.0 0.10802818922296645 9.704168425547193E-4 38-39 0.0 0.0 0.0 0.1815026073021095 9.704168425547193E-4 40-41 0.0 0.0 0.0 0.26067822433126153 9.704168425547193E-4 42-43 0.0 0.0 0.0 0.3428344073768316 9.704168425547193E-4 44-45 0.0 0.0 0.0 0.450862596599798 0.0010050745869316737 46-47 0.0 0.0 0.0 0.5763063023721839 0.001074390075685582 48-49 0.0 0.0 0.0 0.7092534098021805 0.0011956921810049221 50-51 0.0 0.0 0.0 0.8537068883653259 0.0012476787975703534 52-53 0.0 0.0 0.0 0.9928750609109858 0.001316994286324262 54-55 0.0 0.0 0.0 1.144814612259553 0.001316994286324262 56-57 0.0 0.0 0.0 1.325242829485977 0.001334323158512739 58-59 0.0 0.0 0.0 1.5169174847627227 0.0013516520307012164 60-61 0.0 0.0 0.0 1.728225752229013 0.0013516520307012164 62-63 0.0 0.0 0.0 1.9374545550326858 0.0013516520307012164 64-65 0.0 0.0 0.0 2.1591254880676853 0.0013516520307012164 66-67 0.0 0.0 0.0 2.4163206090890625 0.0013516520307012164 68-69 0.0 0.0 0.0 2.700271508769449 0.0013516520307012164 70-71 0.0 0.0 0.0 2.9996624335697684 0.0013863097750781705 72-73 0.0 0.0 0.0 3.3104384273979175 0.0014729541360205562 74-75 0.0 0.0 0.0 3.6212837367148203 0.0015422696247744648 76-77 0.0 0.0 0.0 3.9658856890548764 0.0015595984969629419 78-79 0.0 0.0 0.0 4.345301345621584 0.001594256241339896 80-81 0.0 0.0 0.0 4.739637161142569 0.0016289139857168502 82-83 0.0 0.0 0.0 5.146068529451112 0.0018022027076016218 84-85 0.0 0.0 0.0 5.558894251597202 0.0018022027076016218 86-87 0.0 0.0 0.0 5.990383169090283 0.0018715181963555303 88-89 0.0 0.0 0.0 6.450551370055293 0.0019408336851094387 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4445 0.0 46.754906 1 GTATCAA 7430 0.0 38.980373 1 ATCAACG 9080 0.0 31.268969 3 TATCAAC 9235 0.0 30.847147 2 TCAACGC 9205 0.0 30.741014 4 CAACGCA 9360 0.0 30.231949 5 AACGCAG 9785 0.0 28.99392 6 ACGCAGA 11120 0.0 25.215685 7 CGCAGAG 11160 0.0 25.039267 8 TACATGG 8820 0.0 24.102604 2 TAAGGTG 1900 0.0 24.029387 5 GTACATG 9105 0.0 23.922306 1 GTAAGGT 1870 0.0 23.143276 4 ACATGGG 9090 0.0 22.287985 3 GCAGAGT 12595 0.0 21.506145 9 GAGTACT 6750 0.0 21.047869 12-13 AGAGTAC 10625 0.0 20.148745 10-11 GTACTTT 7250 0.0 19.493923 14-15 GGTAAGG 1990 0.0 19.357843 3 CAGAGTA 11835 0.0 19.353561 10-11 >>END_MODULE