FastQCFastQC Report
Thu 26 May 2016
SRR938471_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938471_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2885358
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54660.189439230764432No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45590.1580046566145345No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA39540.13703672126647715No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA39510.1369327480333463No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA37880.13128353569990275No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC37070.12847625840536947No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA36050.12494116847892012No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG33010.11440521418832601No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30180.10459707252964796No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA29230.10130458681383732No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41350.048.5090941
GTATCAA71000.039.9001121
ATCAACG88950.031.5641633
TCAACGC90650.030.9722274
TATCAAC91900.030.722012
CAACGCA93150.030.089985
AACGCAG97150.028.9343246
ACGCAGA111300.025.1704677
CGCAGAG112550.024.8487328
TAAGGTG20100.024.5804585
GTAAGGT20950.022.2225954
GTGTAAG14550.022.2142751
TAAGGTA9750.021.9260624
TACATGG86150.021.2966212
GTACATG87650.021.1494431
GCAGAGT129550.020.928269
GGTAAGG21400.020.8673253
GAGTACT72950.020.63328612-13
ACATGGG86250.020.3245053
AGAGTAC109000.020.10386810-11
Produced by FastQC (version 0.11.5)