##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938471_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2885358 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.816199237668254 31.0 31.0 34.0 30.0 34.0 2 32.043761987247336 31.0 31.0 34.0 30.0 34.0 3 32.10825485087119 33.0 31.0 34.0 30.0 34.0 4 35.77699127803205 37.0 35.0 37.0 35.0 37.0 5 35.668811287888715 37.0 35.0 37.0 33.0 37.0 6 35.665516376130796 37.0 35.0 37.0 33.0 37.0 7 35.598255051886106 37.0 35.0 37.0 33.0 37.0 8 35.570572178565016 37.0 35.0 37.0 33.0 37.0 9 37.16813268925381 39.0 37.0 39.0 33.0 39.0 10-11 37.0682702111835 39.0 37.0 39.0 33.0 39.0 12-13 36.96637574956037 39.0 37.0 39.0 33.0 39.0 14-15 38.03562140295936 40.0 37.0 41.0 33.0 41.0 16-17 37.94439528821034 40.0 37.0 41.0 33.0 41.0 18-19 38.16366305325024 40.0 37.5 41.0 33.0 41.0 20-21 38.12229123734386 40.0 37.0 41.0 33.0 41.0 22-23 38.02500850847625 40.0 37.0 41.0 33.0 41.0 24-25 37.90355096317337 40.0 37.0 41.0 32.5 41.0 26-27 37.721384833355174 40.0 37.0 41.0 32.0 41.0 28-29 37.61691131568422 40.0 37.0 41.0 32.0 41.0 30-31 37.374129657394334 39.0 36.0 41.0 31.5 41.0 32-33 37.189482899522346 39.0 36.0 41.0 31.0 41.0 34-35 37.1199010660029 39.0 36.0 41.0 31.0 41.0 36-37 37.090483399287024 39.0 36.0 41.0 31.0 41.0 38-39 36.93179511866465 39.0 36.0 41.0 30.5 41.0 40-41 36.77074681893893 39.0 35.0 41.0 30.0 41.0 42-43 36.5668892733588 39.0 35.0 40.5 30.0 41.0 44-45 36.30345610492701 39.0 35.0 40.0 30.0 41.0 46-47 35.97122818035058 38.5 35.0 40.0 29.0 41.0 48-49 35.64505912264613 38.0 34.5 40.0 28.0 41.0 50-51 35.28409680878421 38.0 34.0 40.0 27.0 41.0 52-53 34.79905075903926 38.0 33.5 40.0 26.0 41.0 54-55 34.465942874333095 38.0 33.0 40.0 25.5 41.0 56-57 34.13699634499427 37.0 33.0 40.0 25.0 41.0 58-59 33.67308805354483 37.0 32.5 39.5 23.0 41.0 60-61 33.276465520049854 36.0 32.0 39.0 22.5 40.5 62-63 32.798196272351646 36.0 31.0 39.0 22.0 40.0 64-65 32.2708946342187 35.0 31.0 38.5 20.0 40.0 66-67 31.776832545562804 35.0 30.5 38.0 18.5 40.0 68-69 31.286610361695153 34.5 30.0 37.5 18.0 40.0 70-71 30.71123288687227 34.0 29.0 37.0 13.5 39.0 72-73 30.215666825399136 34.0 29.0 36.0 10.0 39.0 74-75 29.62921273547338 34.0 28.0 36.0 7.5 38.5 76-77 28.40799859150927 31.5 26.5 34.5 7.0 36.5 78-79 29.1024373751888 33.5 28.5 35.0 6.0 37.0 80-81 28.74486389557206 33.0 27.5 35.0 2.0 37.0 82-83 28.306511358382565 33.0 27.0 35.0 2.0 36.0 84-85 27.75760910777796 33.0 26.0 35.0 2.0 36.0 86-87 27.167982274643215 32.5 25.5 35.0 2.0 35.0 88-89 26.432147587924966 32.0 24.5 34.0 2.0 35.0 90-91 25.845247626117796 31.5 23.5 34.0 2.0 35.0 92-93 25.04000283500349 31.0 20.0 34.0 2.0 35.0 94-95 24.24690437027225 31.0 15.5 34.0 2.0 35.0 96-97 23.207798304404513 30.5 2.0 34.0 2.0 35.0 98-99 21.880946662424556 30.0 2.0 34.0 2.0 35.0 100-101 17.95040754041613 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.240703095100951 1101 2 0.325019968972903 1101 3 0.31970210342170446 1101 4 0.2813564991986013 1101 5 0.20427306657037292 1101 6 0.15246850635217868 1101 7 0.2351151973772616 1101 8 0.25261678855537184 1101 9 0.34132149100672393 1101 10-11 0.28991255074103606 1101 12-13 0.3553859837632274 1101 14-15 0.4179509144891185 1101 16-17 0.5205606390789583 1101 18-19 0.5070790296379073 1101 20-21 0.3578015560014194 1101 22-23 0.39356808935639265 1101 24-25 0.5478614398807622 1101 26-27 0.38690703611926835 1101 28-29 0.3372281280170313 1101 30-31 0.3910130206338067 1101 32-33 0.45922096567352355 1101 34-35 0.4829555598845232 1101 36-37 0.5359967656744118 1101 38-39 0.6332598507454037 1101 40-41 0.6030572140173973 1101 42-43 0.6390076804855553 1101 44-45 0.66357471748519 1101 46-47 0.6250551057850373 1101 48-49 0.6916208625636955 1101 50-51 0.6877607604433749 1101 52-53 0.8773486657218115 1101 54-55 0.7790940180359982 1101 56-57 0.7706980672834547 1101 58-59 1.0772790952492883 1101 60-61 1.0324864932977604 1101 62-63 0.9602642904122405 1101 64-65 0.9055133049389781 1101 66-67 0.886102416894694 1101 68-69 1.1268693240542405 1101 70-71 1.0411464797888996 1101 72-73 1.005100116035539 1101 74-75 1.050220902187629 1101 76-77 0.914953580639768 1101 78-79 1.0354242707673258 1101 80-81 1.0866438560607925 1101 82-83 1.16448331793557 1101 84-85 1.107613717570171 1101 86-87 1.3057292200885868 1101 88-89 1.3743786664527207 1101 90-91 1.326059573144235 1101 92-93 1.3269236941248082 1101 94-95 1.2922270950535228 1101 96-97 1.217765135717098 1101 98-99 1.3975742833208393 1101 100-101 1.6724071945847 1102 1 0.31642175887549584 1102 2 0.27434126467407083 1102 3 0.23926898901762428 1102 4 0.19335105922584717 1102 5 0.13054138879194 1102 6 0.19205380682788586 1102 7 0.1853643217324077 1102 8 0.21399027934143788 1102 9 0.3028728855901335 1102 10-11 0.32069049806970895 1102 12-13 0.36451975626704325 1102 14-15 0.5059701301983921 1102 16-17 0.4819378689935121 1102 18-19 0.49269073203424085 1102 20-21 0.4077382728917911 1102 22-23 0.3952573226474385 1102 24-25 0.3910540678412815 1102 26-27 0.36856300297458944 1102 28-29 0.45884564351178625 1102 30-31 0.43582142441035643 1102 32-33 0.41087816709722347 1102 34-35 0.3747344233652754 1102 36-37 0.5071772688876379 1102 38-39 0.5260238105848032 1102 40-41 0.37738880544164743 1102 42-43 0.3558627008296966 1102 44-45 0.4644741943614292 1102 46-47 0.3643239054342686 1102 48-49 0.43853952549858377 1102 50-51 0.5572886607049483 1102 52-53 0.5634646387932634 1102 54-55 0.4859961999421003 1102 56-57 0.5248142901549357 1102 58-59 0.4737749677106322 1102 60-61 0.4142769638205053 1102 62-63 0.6124760111499867 1102 64-65 0.47611887450860735 1102 66-67 0.4917804212549086 1102 68-69 0.611549393069371 1102 70-71 0.6331772555714679 1102 72-73 0.5440465503304601 1102 74-75 0.6208891185590524 1102 76-77 0.5836271753211264 1102 78-79 0.7321644854352876 1102 80-81 0.6876383723992738 1102 82-83 0.8573938778510346 1102 84-85 0.7540310355339805 1102 86-87 0.8092445912521882 1102 88-89 0.8685762606318548 1102 90-91 0.9772530701569337 1102 92-93 1.3984400366746321 1102 94-95 1.3908470680084726 1102 96-97 1.5260710389350685 1102 98-99 1.4797601962001998 1102 100-101 1.5920977338155708 1103 1 0.22450035304431637 1103 2 0.21885505881192557 1103 3 0.2738777115962705 1103 4 0.1583383343634992 1103 5 0.06574619617719435 1103 6 0.12508377557214345 1103 7 0.1566390336086272 1103 8 0.11336226142018546 1103 9 0.2481487199637158 1103 10-11 0.16323121279087616 1103 12-13 0.3373487883065991 1103 14-15 0.4678015910900015 1103 16-17 0.3418289093356819 1103 18-19 0.3804760737669568 1103 20-21 0.32002139382153416 1103 22-23 0.2903558314677568 1103 24-25 0.4219505658430407 1103 26-27 0.4372687925450336 1103 28-29 0.34527261828461775 1103 30-31 0.3414566514741537 1103 32-33 0.47278849310529125 1103 34-35 0.46726546138503267 1103 36-37 0.4546606007694649 1103 38-39 0.3986578570910311 1103 40-41 0.4610390507460238 1103 42-43 0.48769808078537125 1103 44-45 0.41941584385195796 1103 46-47 0.40602202138050103 1103 48-49 0.5127803882732778 1103 50-51 0.5365732621700019 1103 52-53 0.4050159107956617 1103 54-55 0.3475637288028608 1103 56-57 0.43033460638953613 1103 58-59 0.5486811037069899 1103 60-61 0.5463905489416092 1103 62-63 0.6487648510102559 1103 64-65 0.6314879922837378 1103 66-67 0.6585184535944464 1103 68-69 0.5133744189545482 1103 70-71 0.3379991558820947 1103 72-73 0.5211030912967765 1103 74-75 0.5776826467811524 1103 76-77 0.47197618491074067 1103 78-79 0.7268422996619712 1103 80-81 0.8096748825936793 1103 82-83 0.9043222509072315 1103 84-85 1.0147600782179147 1103 86-87 0.8527788612902185 1103 88-89 0.8327845613063225 1103 90-91 0.9357847758690951 1103 92-93 1.105047288394669 1103 94-95 1.0226957534285894 1103 96-97 1.0870233715039426 1103 98-99 1.2720256087663593 1103 100-101 1.7125823430219853 1104 1 0.26014369350390965 1104 2 0.291601209970608 1104 3 0.2634938393181656 1104 4 0.19064721285236885 1104 5 0.08678540170794236 1104 6 0.09681349158182684 1104 7 0.16623155304576898 1104 8 0.20928990580043205 1104 9 0.32304659134707236 1104 10-11 0.3289159975992533 1104 12-13 0.37758203649396194 1104 14-15 0.4700401028762613 1104 16-17 0.5502337625091016 1104 18-19 0.4128959989044816 1104 20-21 0.3619855119727191 1104 22-23 0.4058610673815508 1104 24-25 0.3770921910514744 1104 26-27 0.41785094158495184 1104 28-29 0.44268435549276575 1104 30-31 0.4186357260470288 1104 32-33 0.4168901336801838 1104 34-35 0.4429886917314292 1104 36-37 0.48729845418935724 1104 38-39 0.5642268177335978 1104 40-41 0.5606238232553551 1104 42-43 0.7058331438902528 1104 44-45 0.6111382808855055 1104 46-47 0.6033336634792974 1104 48-49 0.5974619410300477 1104 50-51 0.7203289443528931 1104 52-53 0.6933418883859233 1104 54-55 0.76350527453301 1104 56-57 0.6059380680559627 1104 58-59 0.728619610936228 1104 60-61 0.7262477771290534 1104 62-63 0.6239589407070412 1104 64-65 0.7624610524770219 1104 66-67 0.7162652221553429 1104 68-69 0.8621797211655817 1104 70-71 0.8066277732588283 1104 72-73 0.7613558538041936 1104 74-75 0.8661412923476917 1104 76-77 0.7471171339167455 1104 78-79 0.8518544884557286 1104 80-81 0.8328843671657289 1104 82-83 1.1444451016982633 1104 84-85 1.4186829833011423 1104 86-87 1.2554439931080452 1104 88-89 1.1716096301883212 1104 90-91 1.1569043784384654 1104 92-93 1.5248474754773405 1104 94-95 1.533774264450443 1104 96-97 1.6120669326603263 1104 98-99 1.9390770084808864 1104 100-101 1.908790072407733 1105 1 0.25244995540007054 1105 2 0.23690180391968596 1105 3 0.2263414791508609 1105 4 0.2134849054625576 1105 5 0.1615042379063567 1105 6 0.20480217585411964 1105 7 0.21880187360099512 1105 8 0.21391740436005335 1105 9 0.3070248096968129 1105 10-11 0.2813699975575119 1105 12-13 0.3366509863163003 1105 14-15 0.37719760080040743 1105 16-17 0.37354821597347865 1105 18-19 0.32905623735643275 1105 20-21 0.30425433695060633 1105 22-23 0.3055967226401819 1105 24-25 0.3369757133437332 1105 26-27 0.2748830674555407 1105 28-29 0.3901135073267241 1105 30-31 0.5012832044073647 1105 32-33 0.5219869830932566 1105 34-35 0.5690862101550138 1105 36-37 0.49805638001759434 1105 38-39 0.38850287121410787 1105 40-41 0.5131336913362432 1105 42-43 0.612545690029819 1105 44-45 0.6064416653095464 1105 46-47 0.6801311104057604 1105 48-49 0.7042227576965558 1105 50-51 0.8490822797325777 1105 52-53 0.7715835819744186 1105 54-55 0.8559317847955086 1105 56-57 0.7261659748222087 1105 58-59 0.8871375826501904 1105 60-61 0.8547572976902842 1105 62-63 0.8744231209745337 1105 64-65 0.9190034734415065 1105 66-67 0.770080469732612 1105 68-69 0.8353614394744042 1105 70-71 0.8121762458020783 1105 72-73 0.8008634686351677 1105 74-75 0.8374254876837526 1105 76-77 0.8115377691742367 1105 78-79 1.058737019074158 1105 80-81 1.1158954514481572 1105 82-83 1.1707483766137443 1105 84-85 1.0545068311691352 1105 86-87 1.0565245752245715 1105 88-89 1.0824040080613067 1105 90-91 1.3668703838876297 1105 92-93 1.6158013067173869 1105 94-95 1.5074943216575214 1105 96-97 1.6492877649863154 1105 98-99 1.7253586935619545 1105 100-101 1.7248392077474115 1106 1 0.054167882650276056 1106 2 0.12158216885284645 1106 3 0.08469165773708909 1106 4 0.041593959748482234 1106 5 0.02242558263652228 1106 6 0.03166263852783402 1106 7 0.06114773463175993 1106 8 0.08720878556922429 1106 9 -0.017895246045448232 1106 10-11 0.048314856160104114 1106 12-13 -0.10024337230719027 1106 14-15 7.771229746253994E-4 1106 16-17 0.04176408035828416 1106 18-19 0.07287976004679564 1106 20-21 0.21058064987042258 1106 22-23 0.21704620846465872 1106 24-25 0.18263245429571384 1106 26-27 0.1507693909107104 1106 28-29 0.0915256577458834 1106 30-31 0.1201000617098984 1106 32-33 0.1704010478348934 1106 34-35 0.1850497043740731 1106 36-37 0.2953196929611934 1106 38-39 0.043980342664895034 1106 40-41 0.0868535733052056 1106 42-43 0.16940323724557516 1106 44-45 0.11600929573935304 1106 46-47 -0.6072847412369029 1106 48-49 -0.24394181955858585 1106 50-51 0.13703442097832408 1106 52-53 0.08505053930970519 1106 54-55 0.4492317484850119 1106 56-57 0.41647976396881603 1106 58-59 0.05281557897574629 1106 60-61 0.21264805504223006 1106 62-63 -0.04375182773949149 1106 64-65 0.27429455641064493 1106 66-67 0.2618595938995014 1106 68-69 0.057791433281074944 1106 70-71 0.1236491845583032 1106 72-73 0.43669253092647864 1106 74-75 0.40982793122070404 1106 76-77 0.3533499490480665 1106 78-79 0.5424446182626319 1106 80-81 0.05867404907825957 1106 82-83 0.41696203236025653 1106 84-85 0.7455184373694834 1106 86-87 0.9967794434968837 1106 88-89 0.8991786713236039 1106 90-91 1.0959198868863034 1106 92-93 1.151493102415575 1106 94-95 1.2969852152950132 1106 96-97 1.506208491999498 1106 98-99 1.2620620123595465 1106 100-101 1.2046376579610616 1107 1 0.12634160648450532 1107 2 0.12654515037943526 1107 3 0.150577375437841 1107 4 0.06198111258988348 1107 5 0.03853818562474487 1107 6 0.09845668981780875 1107 7 0.08248680522588359 1107 8 0.11666705752978146 1107 9 0.15270472628520793 1107 10-11 0.188661475729468 1107 12-13 0.23109466481397334 1107 14-15 0.3060090716192718 1107 16-17 0.24628161192296005 1107 18-19 0.2365415958051642 1107 20-21 0.2563256963816656 1107 22-23 0.21528154511765507 1107 24-25 0.1972296821448083 1107 26-27 0.17730240607965442 1107 28-29 0.2791227934739098 1107 30-31 0.2737862418650323 1107 32-33 0.23855627877647834 1107 34-35 0.1937835415007143 1107 36-37 0.355305204528662 1107 38-39 0.39987574463470565 1107 40-41 0.35058877573878533 1107 42-43 0.2307695743565361 1107 44-45 0.38907530626624975 1107 46-47 0.2527823585638984 1107 48-49 0.3962755333440384 1107 50-51 0.41390579123470417 1107 52-53 0.45541439773645465 1107 54-55 0.3785999795259869 1107 56-57 0.41833077018517173 1107 58-59 0.33183000236221716 1107 60-61 0.5347176886678184 1107 62-63 0.6046818003482599 1107 64-65 0.4353274746708564 1107 66-67 0.5267621585303601 1107 68-69 0.6261107652018865 1107 70-71 0.8996277398291284 1107 72-73 0.8434726088694902 1107 74-75 0.6589744905646704 1107 76-77 0.7117174315640469 1107 78-79 0.9597101544485476 1107 80-81 0.8873343016088633 1107 82-83 1.0648064487831626 1107 84-85 1.0823588100404926 1107 86-87 1.2520944827077756 1107 88-89 1.1847052951391142 1107 90-91 1.205906669658539 1107 92-93 1.3726388178116444 1107 94-95 1.5818897742315379 1107 96-97 1.492770371771261 1107 98-99 1.5958861628067957 1107 100-101 1.6221235913373846 1108 1 0.11509114021798084 1108 2 0.008197582797023983 1108 3 0.0687702474943066 1108 4 0.10822958460824594 1108 5 0.026512303997506592 1108 6 0.11554198712226338 1108 7 -0.007882833112766718 1108 8 0.04421723265222255 1108 9 0.062472666848542246 1108 10-11 0.11820008582730424 1108 12-13 0.22261773753862713 1108 14-15 0.24835915179289003 1108 16-17 0.17403912426388501 1108 18-19 0.18834279752134364 1108 20-21 0.16703844229184028 1108 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2316 58-59 -0.795023991072604 2316 60-61 -0.7243365572929648 2316 62-63 -0.8585709608987742 2316 64-65 -0.7899212770368536 2316 66-67 -0.9499103454353204 2316 68-69 -0.7661826981082243 2316 70-71 -0.7376098568404785 2316 72-73 -0.7029233782431916 2316 74-75 -0.8032938658497066 2316 76-77 -0.8268016118877171 2316 78-79 -1.1381862789090569 2316 80-81 -1.0208855639937617 2316 82-83 -1.1163325654232032 2316 84-85 -1.2088422754553214 2316 86-87 -1.1726654300830432 2316 88-89 -1.1241833023498238 2316 90-91 -1.090792264902639 2316 92-93 -1.3627004321806808 2316 94-95 -1.0300051717463212 2316 96-97 -1.0571744947381099 2316 98-99 -1.1522474050104954 2316 100-101 -1.5814499640551105 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 12.0 4 30.0 5 76.0 6 184.0 7 451.0 8 1116.0 9 2030.0 10 3510.0 11 5291.0 12 7469.0 13 10301.0 14 13665.0 15 17710.0 16 21738.0 17 27024.0 18 26142.0 19 24313.0 20 24879.0 21 26570.0 22 29024.0 23 32882.0 24 37924.0 25 44780.0 26 53633.0 27 64260.0 28 76696.0 29 91311.0 30 108525.0 31 128247.0 32 154667.0 33 189703.0 34 241420.0 35 313964.0 36 410428.0 37 458896.0 38 226183.0 39 10297.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.29168435663673 18.88782797204494 14.897421278911189 23.923066392407147 2 16.027938720121448 20.003862847417874 39.014585543172174 24.953612889288504 3 19.476370003306346 23.08067144527646 29.71128712624222 27.73167142517497 4 13.608501960588598 16.657239760196134 35.99664235772476 33.7376159214905 5 14.211281997228268 36.89995932897313 34.18027077264896 14.708487901149642 6 33.61773478369062 35.67068627185951 16.608961522279035 14.102617422170836 7 29.56184293248879 31.932536621105594 19.952879330745095 18.552741115660517 8 26.87766994598244 35.09079289294431 19.210926339123255 18.820610821949998 9 26.278888096381802 16.558049295789292 19.559929824999188 37.603132782829725 10-11 25.352417273697057 26.1755733603941 27.15234989904199 21.31965946686685 12-13 26.21910348733155 23.232559010008462 27.298224345124588 23.25011315753539 14-15 22.644226470337475 24.995303875636925 25.297172829160193 27.063296824865407 16-17 22.751544175800714 28.05324330637654 26.30697819819932 22.888234319623425 18-19 22.845743925017274 26.597098176378807 28.170178535904384 22.386979362699535 20-21 23.083045500766282 25.971803152329798 28.44109812369903 22.504053223204885 22-23 22.913101251213888 25.864710722205007 28.40564325120141 22.816544775379693 24-25 22.911685370086833 26.126264766257073 28.318286546621646 22.643763317034452 26-27 22.466246952218057 26.452927690236177 28.059401205076085 23.021424152469677 28-29 22.617956517286355 26.828029910373203 27.942018196383913 22.611995375956536 30-31 22.738802122820317 26.282265785768438 27.909264594389686 23.069667497021555 32-33 22.350562420037683 26.285483860067266 27.962026011807605 23.40192770808744 34-35 23.15245716282252 26.545861100577678 27.673627744113162 22.62805399248664 36-37 23.330994555060723 25.941407364447635 27.745894976144807 22.981703104346835 38-39 22.706599926383277 26.30184450789439 27.777800884093867 23.21375468162847 40-41 23.1783355858264 26.135337084619515 27.88342650896962 22.802900820584465 42-43 22.92701684546565 26.174251594235848 27.634438024390047 23.264293535908454 44-45 22.85214377924397 26.38706029090464 27.884614884699978 22.876181045151416 46-47 23.33375962358917 26.65431811234516 27.166524916492165 22.845397347573506 48-49 23.01944853983457 26.708852073122298 27.126772483691795 23.144926903351333 50-51 23.117112939432158 26.805097348911378 27.18673308511328 22.89105662654319 52-53 23.51333872607836 26.395130171022107 27.226898707196817 22.864632395702717 54-55 22.923790750568614 26.6951326762699 27.393341275858337 22.987735297303153 56-57 22.961951341913203 26.735122643360025 27.207455712601348 23.09547030212542 58-59 22.90860477281449 26.456947363567885 27.152354497657132 23.482093365960495 60-61 22.490623347258815 26.71292435808659 27.53183487109745 23.264617423557148 62-63 22.781280388122234 26.724216964542112 27.528044808610396 22.96645783872526 64-65 22.44751065801154 26.73026521715168 27.331096644307863 23.491127480528917 66-67 22.363900661728316 26.79594145513682 27.308059970081505 23.532097913053363 68-69 23.018404678304194 26.310954848735214 27.39718287498854 23.273457597972055 70-71 22.695984617485408 26.427987692324756 27.091875035091018 23.78415265509882 72-73 22.688284326688173 26.577283157682047 27.102885362192612 23.631547153437165 74-75 22.67408464580236 26.847911206110297 26.913290553654917 23.564713594432423 76-77 22.70994153101228 26.425766716298156 27.244703566878712 23.61958818581085 78-79 22.754826284943654 26.60370293971326 27.187572045422638 23.453898729920454 80-81 22.908092755369594 26.62526517988552 26.912306860036157 23.55433520470873 82-83 22.8046097760119 26.720527128719052 26.976966721619245 23.497896373649805 84-85 22.636848859127774 26.75345887445587 27.003606366672233 23.606085899744116 86-87 22.848680527754237 26.810546072778507 26.69262804348617 23.648145355981082 88-89 22.637446152853574 26.84278731803662 26.510989344517327 24.008777184592475 90-91 22.46644157741356 26.843964753234683 26.729998802274192 23.95959486707757 92-93 22.61528509668234 26.504314383740436 26.95086722735802 23.9295332922192 94-95 22.294473706150992 26.20993790994628 27.150154761465235 24.345433622437493 96-97 22.541067938898347 25.873637937846087 27.145777163103872 24.439516960151693 98-99 22.239608383130957 26.202988446997217 26.78710566019809 24.770297509673735 100-101 22.38984225272632 25.881866845342227 27.143907427941947 24.584383473989497 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 140.0 1 106.5 2 115.5 3 166.5 4 145.5 5 147.5 6 269.0 7 605.0 8 967.5 9 1094.0 10 1022.0 11 926.0 12 959.5 13 1093.5 14 1241.0 15 1385.0 16 1606.0 17 1872.0 18 2100.0 19 2445.0 20 2897.0 21 3500.0 22 4331.0 23 5449.5 24 6909.5 25 8842.0 26 11828.5 27 15618.5 28 19226.5 29 23063.5 30 27680.0 31 34569.0 32 44978.5 33 49427.5 34 49074.0 35 53215.5 36 59906.5 37 66523.0 38 71810.0 39 76206.0 40 81724.5 41 87833.0 42 91449.0 43 94968.0 44 98960.0 45 104452.5 46 107956.5 47 110786.5 48 112518.0 49 110855.5 50 109779.0 51 108427.0 52 107191.0 53 107933.5 54 108970.5 55 102783.5 56 94212.0 57 86479.5 58 79571.0 59 70113.5 60 56520.5 61 46591.5 62 39457.0 63 30008.0 64 20959.0 65 15437.5 66 11325.0 67 8739.5 68 6819.0 69 5229.0 70 3964.0 71 2642.0 72 1878.5 73 1537.5 74 1149.5 75 833.5 76 663.0 77 493.5 78 320.5 79 200.0 80 108.0 81 54.5 82 31.5 83 16.5 84 9.5 85 4.5 86 2.0 87 2.0 88 2.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.5055067690040544E-4 2 0.05112017295600754 3 0.0 4 0.0 5 0.04301026077180024 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006602300303809787 26-27 0.002183437895748119 28-29 1.559598496962942E-4 30-31 0.001576927369151419 32-33 5.025372934658368E-4 34-35 0.012615418953211352 36-37 0.02290876903316677 38-39 0.0049907151902814145 40-41 0.0012650076697588305 42-43 0.001438296391643602 44-45 0.00882039594393486 46-47 0.0 48-49 0.0 50-51 5.19866165654314E-4 52-53 0.0 54-55 0.001576927369151419 56-57 0.0 58-59 0.0223542451231355 60-61 0.0 62-63 0.00996410150837435 64-65 0.015214749781482923 66-67 0.006446340454113493 68-69 0.016167837751849164 70-71 1.3863097750781706E-4 72-73 3.4657744376954265E-5 74-75 0.022700822566905043 76-77 0.04976852092530632 78-79 0.030949365728620157 80-81 0.026322556854296766 82-83 0.03649460482893284 84-85 0.01594256241339896 86-87 0.010501296546217143 88-89 0.08418366109162191 90-91 0.025109535801103363 92-93 0.024433709785752756 94-95 0.026253241365542853 96-97 0.03751700828805299 98-99 0.05264511370859353 100-101 0.01479885684895947 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2885358.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.044108774760666 #Duplication Level Percentage of deduplicated Percentage of total 1 73.60823772928698 38.30875131101453 2 14.487746603746245 15.080037202930782 3 5.048935244768298 7.8830200522633245 4 2.3415824110415104 4.874622788212428 5 1.268089006805991 3.29982811031446 6 0.7569645186893156 2.363732624958066 7 0.4921559039864798 1.7929670780849112 8 0.339790703901651 1.4147283483608015 9 0.2484119152411881 1.163553906398312 >10 1.270973165581582 11.969003666291117 >50 0.08850989515920482 3.165805458028909 >100 0.04293788368838682 4.241126508255917 >500 0.0023993017848538503 0.9031188256279963 >1k 0.003199069046368045 3.350110560063271 >5k 6.664727179933426E-5 0.18959355919516524 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5466 0.189439230764432 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4559 0.1580046566145345 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3954 0.13703672126647715 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3951 0.1369327480333463 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3788 0.13128353569990275 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3707 0.12847625840536947 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3605 0.12494116847892012 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3301 0.11440521418832601 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3018 0.10459707252964796 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2923 0.10130458681383732 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0397323313086279E-4 2 0.0 0.0 0.0 0.0 1.0397323313086279E-4 3 0.0 0.0 0.0 0.0 1.0397323313086279E-4 4 0.0 0.0 0.0 0.0 1.0397323313086279E-4 5 0.0 0.0 0.0 0.0 1.0397323313086279E-4 6 0.0 0.0 0.0 0.0 1.0397323313086279E-4 7 0.0 0.0 0.0 0.0 1.0397323313086279E-4 8 0.0 0.0 0.0 0.0 1.0397323313086279E-4 9 0.0 0.0 0.0 0.0 1.0397323313086279E-4 10-11 0.0 0.0 0.0 0.0 1.0397323313086279E-4 12-13 0.0 0.0 0.0 1.7328872188477133E-5 3.98564060334974E-4 14-15 0.0 0.0 0.0 3.4657744376954265E-5 5.025372934658369E-4 16-17 0.0 0.0 0.0 6.931548875390853E-5 5.545239100312682E-4 18-19 0.0 0.0 0.0 8.664436094238566E-5 6.238393987851767E-4 20-21 0.0 0.0 0.0 2.4260421063867983E-4 8.144569928584251E-4 22-23 0.0 0.0 0.0 4.505506769004054E-4 9.011013538008109E-4 24-25 0.0 0.0 0.0 6.58497143162131E-4 9.011013538008109E-4 26-27 0.0 0.0 0.0 0.001039732331308628 9.357590981777652E-4 28-29 0.0 0.0 0.0 0.003032552632983498 9.704168425547193E-4 30-31 0.0 0.0 0.0 0.011367740155640998 9.704168425547193E-4 32-33 0.0 0.0 0.0 0.030741419262358433 0.001039732331308628 34-35 0.0 0.0 0.0 0.0597846090502461 0.001039732331308628 36-37 0.0 0.0 0.0 0.10941449899804462 0.001039732331308628 38-39 0.0 0.0 0.0 0.1835300853481613 0.001057061203497105 40-41 0.0 0.0 3.4657744376954265E-5 0.26301762207670587 0.001074390075685582 42-43 0.0 0.0 3.4657744376954265E-5 0.3457629867766842 0.001074390075685582 44-45 0.0 0.0 3.4657744376954265E-5 0.4548828949475247 0.0011090478200625363 46-47 0.0 0.0 3.4657744376954265E-5 0.5818342126003082 0.001178363308816445 48-49 0.0 0.0 3.4657744376954265E-5 0.7152838573237705 0.0012476787975703534 50-51 0.0 0.0 3.4657744376954265E-5 0.8597893225034814 0.001316994286324262 52-53 0.0 0.0 3.4657744376954265E-5 0.9997372942976226 0.0014556252638320791 54-55 0.0 0.0 3.4657744376954265E-5 1.1515555435408709 0.0014729541360205562 56-57 0.0 0.0 3.4657744376954265E-5 1.3317584854288445 0.0015249407525859877 58-59 0.0 0.0 3.4657744376954265E-5 1.523797047021548 0.0015595984969629419 60-61 0.0 0.0 3.4657744376954265E-5 1.7351226433600266 0.0016115851135283733 62-63 0.0 0.0 3.4657744376954265E-5 1.9461709777434897 0.0017328872188477132 64-65 0.0 0.0 3.4657744376954265E-5 2.1685350656660285 0.0017848738354131447 66-67 0.0 0.0 3.4657744376954265E-5 2.427185811951238 0.0018022027076016218 68-69 0.0 0.0 3.4657744376954265E-5 2.7132854917829956 0.0018022027076016218 70-71 0.0 0.0 3.4657744376954265E-5 3.0153623917725287 0.001854189324167053 72-73 0.0 0.0 3.4657744376954265E-5 3.330366630414666 0.002096793534805733 74-75 0.0 0.0 3.4657744376954265E-5 3.646271970410604 0.002339397745444413 76-77 0.0 0.0 3.4657744376954265E-5 3.9974935519266586 0.0023913843620098443 78-79 0.0 0.0 3.4657744376954265E-5 4.386093510753259 0.002408713234198321 80-81 0.0 0.0 3.4657744376954265E-5 4.791346515752984 0.002512686467329184 82-83 0.0 0.0 3.4657744376954265E-5 5.208816375645587 0.002737961805779387 84-85 0.0 0.0 3.4657744376954265E-5 5.63413621463957 0.002737961805779387 86-87 0.0 0.0 3.4657744376954265E-5 6.080337344620667 0.0029112505276641582 88-89 0.0 0.0 3.4657744376954265E-5 6.557401195969443 0.0030845392495489296 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4135 0.0 48.509094 1 GTATCAA 7100 0.0 39.900112 1 ATCAACG 8895 0.0 31.564163 3 TCAACGC 9065 0.0 30.972227 4 TATCAAC 9190 0.0 30.72201 2 CAACGCA 9315 0.0 30.08998 5 AACGCAG 9715 0.0 28.934324 6 ACGCAGA 11130 0.0 25.170467 7 CGCAGAG 11255 0.0 24.848732 8 TAAGGTG 2010 0.0 24.580458 5 GTAAGGT 2095 0.0 22.222595 4 GTGTAAG 1455 0.0 22.214275 1 TAAGGTA 975 0.0 21.926062 4 TACATGG 8615 0.0 21.296621 2 GTACATG 8765 0.0 21.149443 1 GCAGAGT 12955 0.0 20.92826 9 GGTAAGG 2140 0.0 20.867325 3 GAGTACT 7295 0.0 20.633286 12-13 ACATGGG 8625 0.0 20.324505 3 AGAGTAC 10900 0.0 20.103868 10-11 >>END_MODULE