FastQCFastQC Report
Thu 26 May 2016
SRR938470_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938470_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2906319
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57480.1977759495774552No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45890.15789732648067883No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA40060.13783758768393972No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA39160.13474088701205889No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA35870.12342072566707232No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA35040.12056487949189336No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC32350.11130918526149401No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG29490.10146855868196161No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29490.10146855868196161No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA29080.10005783948699369No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42350.046.4358181
GTATCAA72750.039.0748941
ATCAACG87250.032.1391563
TCAACGC88500.031.6315044
CAACGCA90500.030.9833835
TATCAAC91300.030.7655432
AACGCAG94050.030.1113766
ACGCAGA107650.025.9073127
CGCAGAG109000.025.5428548
TAAGGTG19450.023.945945
TGGTATC21550.023.377952
GCAGAGT121850.021.95169
GTACATG86200.021.6538581
TACATGG83450.021.5854322
GTGTAGC17350.021.4067941
GTAAGGT20600.020.9952984
GTGGTAT24800.020.9281711
TAAGGTA9750.020.473524
GAGTACT68700.020.46720312-13
AGAGTAC104350.019.8961310-11
Produced by FastQC (version 0.11.5)