##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938470_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2906319 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.932356702756994 31.0 31.0 34.0 27.0 34.0 2 31.039369043797326 31.0 31.0 34.0 27.0 34.0 3 31.104905552349898 31.0 31.0 34.0 27.0 34.0 4 34.73114823252369 37.0 35.0 37.0 32.0 37.0 5 34.74293496343657 37.0 35.0 37.0 32.0 37.0 6 34.767472875482696 37.0 35.0 37.0 32.0 37.0 7 34.678417269405045 37.0 35.0 37.0 30.0 37.0 8 34.6246237938781 37.0 35.0 37.0 30.0 37.0 9 36.11540956102892 39.0 35.0 39.0 30.0 39.0 10-11 35.958826611944524 39.0 35.0 39.0 30.0 39.0 12-13 35.813425504908444 38.0 35.0 39.0 30.0 39.0 14-15 36.80089677010679 39.0 36.0 41.0 30.0 41.0 16-17 36.65394025913879 39.0 36.0 41.0 30.0 41.0 18-19 36.62340558624156 39.0 36.0 41.0 30.0 41.0 20-21 36.69116707422688 39.0 36.0 41.0 30.0 41.0 22-23 36.6151619970141 39.0 36.0 41.0 30.0 41.0 24-25 36.50047293500817 39.0 36.0 41.0 29.5 41.0 26-27 36.31453068297046 39.0 35.5 41.0 29.0 41.0 28-29 36.133438896418454 39.0 35.0 40.5 28.5 41.0 30-31 35.9077291584303 39.0 35.0 40.0 27.0 41.0 32-33 35.6319481791228 38.0 34.5 40.0 27.0 41.0 34-35 35.388174697959855 38.0 34.0 40.0 26.0 41.0 36-37 35.099647182570116 38.0 34.0 40.0 25.0 41.0 38-39 34.839125711940085 38.0 34.0 40.0 25.0 41.0 40-41 34.779905612563525 38.0 33.5 40.0 24.5 41.0 42-43 34.60571465141989 38.0 33.0 40.0 24.0 41.0 44-45 34.33610195577292 38.0 33.0 40.0 23.5 41.0 46-47 34.00707269229565 38.0 33.0 40.0 22.5 41.0 48-49 33.655545038242536 38.0 33.0 40.0 20.5 41.0 50-51 32.918022075346855 37.0 31.5 39.5 19.0 40.5 52-53 32.98017234171472 37.0 31.5 39.5 19.0 40.0 54-55 33.10928033020463 37.0 32.0 40.0 18.5 41.0 56-57 32.8064138864316 37.0 31.5 40.0 15.5 41.0 58-59 32.479490035333356 36.5 31.0 39.5 13.0 41.0 60-61 32.03019334766762 36.0 31.0 39.0 9.0 41.0 62-63 31.500201629621525 35.5 30.0 39.0 7.5 40.0 64-65 30.99698966286908 35.0 29.0 38.5 4.5 40.0 66-67 30.46723965951432 35.0 29.0 38.0 2.0 40.0 68-69 29.911573712314443 34.0 28.0 37.5 2.0 40.0 70-71 29.28072348561875 34.0 27.5 37.0 2.0 39.0 72-73 28.731827442204384 34.0 26.0 36.0 2.0 39.0 74-75 28.132602959275978 33.5 26.0 36.0 2.0 39.0 76-77 27.41191675793332 33.0 25.5 35.0 2.0 37.5 78-79 26.650246755431873 32.0 23.5 35.0 2.0 37.0 80-81 26.0201887679914 32.0 21.5 35.0 2.0 36.5 82-83 25.374447367959263 31.5 19.5 35.0 2.0 36.0 84-85 24.737040221668714 31.0 17.5 34.0 2.0 35.5 86-87 24.093614637622366 31.0 10.5 34.0 2.0 35.0 88-89 23.317226016827473 30.0 4.5 34.0 2.0 35.0 90-91 22.516050715699137 29.5 2.0 34.0 2.0 35.0 92-93 21.702263757006715 29.0 2.0 34.0 2.0 35.0 94-95 20.766311956808593 29.0 2.0 34.0 2.0 35.0 96-97 19.566991613790503 26.5 2.0 33.5 2.0 35.0 98-99 18.133864176644064 24.5 2.0 33.0 2.0 34.5 100-101 14.779562566944648 2.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.653053146530965 1101 2 0.6376285524565191 1101 3 0.6836189524788168 1101 4 0.591919423075673 1101 5 0.6081523257052197 1101 6 0.7124231583199219 1101 7 0.4875364372509452 1101 8 0.5811722123820786 1101 9 0.7134467801718003 1101 10-11 0.7540815270679531 1101 12-13 0.7299165171097783 1101 14-15 0.9178595276368 1101 16-17 0.8900622009095756 1101 18-19 0.7325688991479709 1101 20-21 0.8361054111547901 1101 22-23 0.9492555227305886 1101 24-25 1.036246577189047 1101 26-27 1.0173309343548382 1101 28-29 0.9538606427080367 1101 30-31 0.8895617383809551 1101 32-33 1.0259301085887031 1101 34-35 1.1053933738599326 1101 36-37 1.0135583364985692 1101 38-39 0.9306782484225238 1101 40-41 0.8484089829637682 1101 42-43 1.140485296612681 1101 44-45 0.9626938510979386 1101 46-47 1.0112925286156553 1101 48-49 1.1585649985731976 1101 50-51 1.0324512057848736 1101 52-53 1.0600062551012925 1101 54-55 1.2949040142670256 1101 56-57 1.0915792023968933 1101 58-59 1.0982086701824372 1101 60-61 1.1810844894781596 1101 62-63 1.081109590053746 1101 64-65 0.9576934743762919 1101 66-67 0.7998049282519162 1101 68-69 0.8766118494560331 1101 70-71 1.114170624022968 1101 72-73 1.0813538286704194 1101 74-75 1.041124410017698 1101 76-77 1.2649624523665217 1101 78-79 1.2120881844566185 1101 80-81 1.342478086107942 1101 82-83 1.3394138646261595 1101 84-85 1.3777026286489402 1101 86-87 1.4140318411001687 1101 88-89 1.457813510186071 1101 90-91 1.4859412418456976 1101 92-93 1.1615361149423187 1101 94-95 1.3950286336825641 1101 96-97 1.3389320035519319 1101 98-99 1.1849588506581377 1101 100-101 1.162671755849134 1102 1 0.6108172484967795 1102 2 0.6737863003843785 1102 3 0.6784583515444282 1102 4 0.7281967533403488 1102 5 0.7459534690290255 1102 6 0.7165689002872213 1102 7 0.6429114113988916 1102 8 0.6811867955938808 1102 9 0.7407021401650127 1102 10-11 0.756715387694868 1102 12-13 0.8958151346212944 1102 14-15 0.8594034522707616 1102 16-17 0.8840535199213022 1102 18-19 1.0622277050215558 1102 20-21 0.897560590316175 1102 22-23 0.9646309343748882 1102 24-25 0.8854781082141656 1102 26-27 0.7198025236067451 1102 28-29 0.6086168246640966 1102 30-31 0.5088593711279614 1102 32-33 0.6547430881775043 1102 34-35 0.6581766461205447 1102 36-37 0.7528082556280324 1102 38-39 0.8140585573745014 1102 40-41 0.7682699916977853 1102 42-43 0.8713956072085765 1102 44-45 0.7704967311496915 1102 46-47 0.7349973405452559 1102 48-49 0.6651613829972689 1102 50-51 0.7423492293619489 1102 52-53 0.8390001328038252 1102 54-55 0.6664487210914416 1102 56-57 0.6613180435883734 1102 58-59 0.7925804121603477 1102 60-61 0.711427778284083 1102 62-63 0.5497008650506992 1102 64-65 0.6894641281814486 1102 66-67 0.6692966502905975 1102 68-69 0.47493053770938687 1102 70-71 0.4675868739633131 1102 72-73 0.6359098891880954 1102 74-75 0.6057369871098608 1102 76-77 0.7547253691149436 1102 78-79 0.6842257651282964 1102 80-81 0.7336997880483018 1102 82-83 0.9148344577121286 1102 84-85 0.7426262258765384 1102 86-87 0.776563990453468 1102 88-89 0.7965435378146282 1102 90-91 0.8231121252701534 1102 92-93 1.0501795713226052 1102 94-95 1.0508527044668234 1102 96-97 1.3495852386386105 1102 98-99 1.4970798860131467 1102 100-101 1.0238707344940092 1103 1 0.6188374535419001 1103 2 0.6668998456224102 1103 3 0.5210133401608559 1103 4 0.631093006039066 1103 5 0.6497733333987554 1103 6 0.6216767275026029 1103 7 0.49004171397545093 1103 8 0.5140249850232195 1103 9 0.5693732629988375 1103 10-11 0.648830070650483 1103 12-13 0.7330156199109581 1103 14-15 0.8663604864752372 1103 16-17 0.8582947109286039 1103 18-19 0.850964173029169 1103 20-21 0.7775326016959099 1103 22-23 0.7985963472486333 1103 24-25 0.8535441419054521 1103 26-27 0.7920218023526715 1103 28-29 0.8497528657396671 1103 30-31 0.7458917583256337 1103 32-33 0.9443847391349678 1103 34-35 0.8829433756240306 1103 36-37 0.8698832976365551 1103 38-39 0.7275505568779508 1103 40-41 0.9380614144078621 1103 42-43 0.7866547163078863 1103 44-45 0.8703596035474419 1103 46-47 0.8148903259852815 1103 48-49 0.6428271806985464 1103 50-51 0.7034700027539458 1103 52-53 0.7579842111566037 1103 54-55 0.5933725708000992 1103 56-57 0.6853478162112268 1103 58-59 0.5505231909811386 1103 60-61 0.5690516378469894 1103 62-63 0.7678808624655566 1103 64-65 0.6314075776029284 1103 66-67 0.7202126473164014 1103 68-69 0.837500269302744 1103 70-71 0.9798072917065852 1103 72-73 0.8995818246773375 1103 74-75 0.8619729975988726 1103 76-77 0.6992603574498197 1103 78-79 0.6243280061375742 1103 80-81 0.6975912684761063 1103 82-83 0.6851725351613851 1103 84-85 0.9790716455074779 1103 86-87 0.9197254631642444 1103 88-89 0.8481529794627676 1103 90-91 1.0234280370631303 1103 92-93 1.0133438211392836 1103 94-95 0.8621548215941424 1103 96-97 1.0632889124809601 1103 98-99 1.2586083308722387 1103 100-101 1.0615555496117022 1104 1 0.6183227078659357 1104 2 0.6024853749509127 1104 3 0.6427362443843663 1104 4 0.7083947297758968 1104 5 0.523089818728991 1104 6 0.5941588938423479 1104 7 0.5647094679260434 1104 8 0.5553378558690767 1104 9 0.6334644870993174 1104 10-11 0.6726664442157642 1104 12-13 0.6490034687784956 1104 14-15 0.8837416015289747 1104 16-17 0.8249872128325748 1104 18-19 0.8050983007654366 1104 20-21 0.9499379969750379 1104 22-23 0.9330964292846957 1104 24-25 0.9011841970532757 1104 26-27 0.8416328044905086 1104 28-29 0.8372276733222463 1104 30-31 0.860916724225568 1104 32-33 0.7994473436697334 1104 34-35 0.7836848836672345 1104 36-37 0.7000982810946539 1104 38-39 0.8450759084698163 1104 40-41 0.8191453679093286 1104 42-43 0.8110562816049196 1104 44-45 0.7895679188205946 1104 46-47 0.9318162021181777 1104 48-49 0.9555604018751112 1104 50-51 0.7384375678991546 1104 52-53 0.7200009024340872 1104 54-55 0.9547143624187058 1104 56-57 1.0230261222032837 1104 58-59 0.997936851087367 1104 60-61 0.9648745330097981 1104 62-63 1.0443153303453805 1104 64-65 1.0883212585242745 1104 66-67 1.1015904613369791 1104 68-69 1.1458804366563093 1104 70-71 1.0763443903727037 1104 72-73 1.0300707106276157 1104 74-75 1.0567459536153017 1104 76-77 1.078742526238404 1104 78-79 1.0912716363434996 1104 80-81 1.1508287543034577 1104 82-83 1.1784734948608637 1104 84-85 1.1193909004731069 1104 86-87 1.3052740150440947 1104 88-89 1.1426666035710866 1104 90-91 0.976584221480195 1104 92-93 1.1042936518021556 1104 94-95 1.0494032341351307 1104 96-97 0.8838062539248419 1104 98-99 0.9026327996581287 1104 100-101 0.9136243276662004 1105 1 0.4707193080377827 1105 2 0.5727183003358327 1105 3 0.5039726299768965 1105 4 0.4623739685302226 1105 5 0.42748010228023503 1105 6 0.39146968550998196 1105 7 0.4048153642127019 1105 8 0.3225123292904968 1105 9 0.5034884066553005 1105 10-11 0.6515440829143557 1105 12-13 0.5716300914765284 1105 14-15 0.6964236107716815 1105 16-17 0.6197626476808153 1105 18-19 0.507067019559706 1105 20-21 0.5314322725376144 1105 22-23 0.6829692840720938 1105 24-25 0.6902874459327748 1105 26-27 0.7334850605923151 1105 28-29 0.6699318604056899 1105 30-31 0.7231569183687583 1105 32-33 0.703372589251579 1105 34-35 0.7707056216900412 1105 36-37 0.6730999531412891 1105 38-39 0.6184683053022155 1105 40-41 0.6531999648764213 1105 42-43 0.9126255670372174 1105 44-45 0.8996373552015413 1105 46-47 0.7131339838696462 1105 48-49 0.6964820014663928 1105 50-51 0.6468512136574986 1105 52-53 0.6287379266555391 1105 54-55 0.7182979995845855 1105 56-57 0.7696866735142223 1105 58-59 0.8234883942470717 1105 60-61 0.9329355394889802 1105 62-63 0.9161134771603621 1105 64-65 0.8947255454660592 1105 66-67 0.9461040583272933 1105 68-69 0.8137150201541381 1105 70-71 0.8088593110666054 1105 72-73 0.9230423090577524 1105 74-75 0.8584267088230284 1105 76-77 0.9071899612724259 1105 78-79 0.9447974485633743 1105 80-81 0.9327416220956586 1105 82-83 1.1223502046446576 1105 84-85 1.0851302005358079 1105 86-87 1.1444068730826906 1105 88-89 1.2369021066945614 1105 90-91 1.2702594728677603 1105 92-93 1.3468071791238607 1105 94-95 1.6217436349618666 1105 96-97 1.7073925617207628 1105 98-99 1.633396135391159 1105 100-101 1.2084972985679432 1106 1 0.3608649145692233 1106 2 0.4256790495838878 1106 3 0.21306306514187057 1106 4 0.3278923744741604 1106 5 0.3542725880403097 1106 6 0.11686900416933099 1106 7 0.22234617300440362 1106 8 0.33227798527920527 1106 9 0.4459890129714097 1106 10-11 0.14243052840597414 1106 12-13 0.25008733995733934 1106 14-15 0.5098204769673416 1106 16-17 0.30979747402508195 1106 18-19 0.011757243024028696 1106 20-21 0.32559208839884946 1106 22-23 0.40939985011068103 1106 24-25 0.4675167626201784 1106 26-27 0.1822707284126821 1106 28-29 0.30745189150346874 1106 30-31 0.3732953511039341 1106 32-33 0.4949518783997675 1106 34-35 0.3285133866161871 1106 36-37 0.1467818105765346 1106 38-39 0.18294736076370555 1106 40-41 0.3801993347609809 1106 42-43 -0.05164014448325105 1106 44-45 0.20389675423460574 1106 46-47 0.017364313728279512 1106 48-49 0.42934813140163186 1106 50-51 0.15385150151598737 1106 52-53 0.32018507288992026 1106 54-55 0.013500905822048992 1106 56-57 -0.3318223169637875 1106 58-59 -0.6490322828323656 1106 60-61 0.055852985300184343 1106 62-63 0.17847065288323805 1106 64-65 0.20294101548004306 1106 66-67 -0.422826264051551 1106 68-69 -0.523491993166683 1106 70-71 -0.4124168880226762 1106 72-73 0.18924715550678073 1106 74-75 0.16239762604897834 1106 76-77 0.07730498000589492 1106 78-79 0.3846447135677131 1106 80-81 0.270653605395335 1106 82-83 0.2527086791306523 1106 84-85 0.3170648135928005 1106 86-87 0.6681965641221304 1106 88-89 0.31775515380135744 1106 90-91 0.3390830382785026 1106 92-93 0.3852836030600919 1106 94-95 0.16657566839878157 1106 96-97 0.34096558824680656 1106 98-99 -0.03277788526665404 1106 100-101 -0.35431359426936737 1107 1 0.3846304880287654 1107 2 0.333455023084138 1107 3 0.3185253996919357 1107 4 0.35761206376831467 1107 5 0.3701964937922497 1107 6 0.21920623751793755 1107 7 0.27900511366217984 1107 8 0.3110661584713412 1107 9 0.2933910846521215 1107 10-11 0.32420570617357214 1107 12-13 0.3265065892853585 1107 14-15 0.29855808412524 1107 16-17 0.27631656004285077 1107 18-19 0.4215132954498557 1107 20-21 0.3368341252893785 1107 22-23 0.4056640703620715 1107 24-25 0.4703654392520136 1107 26-27 0.3388773912857417 1107 28-29 0.2042483736249281 1107 30-31 0.28460932777786496 1107 32-33 0.31791408572512836 1107 34-35 0.2704306859365744 1107 36-37 0.4389325093871079 1107 38-39 0.5869540134587865 1107 40-41 0.5860546779669491 1107 42-43 0.6000359830745481 1107 44-45 0.5708798634211547 1107 46-47 0.614118696040812 1107 48-49 0.5882760667067117 1107 50-51 0.3457028753114031 1107 52-53 0.38876728086297874 1107 54-55 0.5228137536659361 1107 56-57 0.5502658335592159 1107 58-59 0.6131766617578123 1107 60-61 0.6251037012282552 1107 62-63 0.7097378571320796 1107 64-65 0.4494284572458298 1107 66-67 0.4661135147162696 1107 68-69 0.4643985288246846 1107 70-71 0.41774623717105186 1107 72-73 0.300037891698274 1107 74-75 0.2933500070013615 1107 76-77 0.5544912785999436 1107 78-79 0.2786827329336248 1107 80-81 0.23141750874157552 1107 82-83 0.32131054910238177 1107 84-85 0.44256966432353195 1107 86-87 0.39608144196328965 1107 88-89 0.49836217752991985 1107 90-91 0.4857014058237148 1107 92-93 0.44082874932222893 1107 94-95 0.30044727786059866 1107 96-97 0.5686237748091045 1107 98-99 0.6546830798830321 1107 100-101 0.5024950088959841 1108 1 0.28740029471578765 1108 2 0.3871743517826651 1108 3 0.3644770158456758 1108 4 0.41684816839958927 1108 5 0.39712379701850864 1108 6 0.2903763964810153 1108 7 0.48046053186642723 1108 8 0.21757367884928414 1108 9 0.2570201159824492 1108 10-11 0.33224450681591833 1108 12-13 0.3578847436536776 1108 14-15 0.4236076682153822 1108 16-17 0.30300483706442805 1108 18-19 0.36784264543918255 1108 20-21 0.44397495859182357 1108 22-23 0.5036524582048898 1108 24-25 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-0.9178144624901634 2316 90-91 -0.8486336755135504 2316 92-93 -1.0320680801116389 2316 94-95 -1.133944810015194 2316 96-97 -1.2635749040883262 2316 98-99 -1.4458005541958627 2316 100-101 -1.1101214448360448 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11212.0 3 4208.0 4 4267.0 5 4690.0 6 5567.0 7 6884.0 8 8738.0 9 10935.0 10 13460.0 11 16144.0 12 19082.0 13 21692.0 14 24468.0 15 28082.0 16 31549.0 17 35511.0 18 34830.0 19 34316.0 20 35832.0 21 38904.0 22 42947.0 23 47856.0 24 53423.0 25 59255.0 26 66910.0 27 75728.0 28 86158.0 29 98251.0 30 114708.0 31 133091.0 32 158093.0 33 188819.0 34 232406.0 35 286588.0 36 342894.0 37 344959.0 38 172996.0 39 10866.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.540122760970476 18.13044266615924 14.32506630650531 24.00436826636497 2 15.565483690181612 19.339035470016622 40.15097374208056 24.94450709772121 3 19.531223374939717 22.948181414791375 29.252857993137855 28.267737217131057 4 13.126793186047935 15.999953180071511 36.330680892580624 34.54257274129993 5 14.26604070581722 36.78176432271045 34.048877828839466 14.903317142632863 6 33.44876750664596 35.33680140834741 16.991823087546226 14.2226079974604 7 29.566027020049912 31.824042681352722 20.055141554083125 18.55478874451424 8 26.796234265267323 34.83807147902013 19.354357951314743 19.01133630439781 9 26.35488006036223 16.77010444978672 19.634866346680766 37.24014914317028 10-11 25.317763755534727 26.080723783651116 27.164158348155137 21.43735411265902 12-13 26.251828858277833 23.246262382495694 27.2465074637038 23.255401295522674 14-15 22.80045303278464 24.92447876017297 25.35701458210032 26.91805362494207 16-17 22.792882345227184 27.962175842474835 26.29015065023393 22.954791162064048 18-19 22.91027021668314 26.550564878354216 28.135848937099983 22.403315967862664 20-21 23.183706672739383 25.905257046405833 28.42446966594734 22.486566614907446 22-23 22.895468099476947 25.831172023296016 28.312002517148766 22.961357360078267 24-25 22.842248698267483 26.066310241443514 28.357600490995686 22.733840569293314 26-27 22.421068395341393 26.36696009611909 28.06326233419285 23.148709174346667 28-29 22.562483326018295 26.8116593085999 27.94542121705006 22.680436148331744 30-31 22.697934654779708 26.302597586804268 27.860144400653287 23.139323357762738 32-33 22.29831436566538 26.269862949798977 27.94615072268889 23.485671961846748 34-35 23.143367817124712 26.52917902964119 27.65247553826489 22.674977614969208 36-37 23.281513024499898 25.92023505031137 27.689807945857186 23.108443979331543 38-39 22.673042320649024 26.301706537735605 27.6961671735159 23.329083968099475 40-41 23.195433752394745 26.07957342775394 27.87171100576976 22.85328181408156 42-43 22.882832438046783 26.157865601534645 27.60198514599248 23.35731681442609 44-45 22.824719196231793 26.383528243475784 27.851814613598258 22.939937946694165 46-47 23.329827380123845 26.64755569450134 27.112815701393956 22.909801223980857 48-49 23.018910906199423 26.700783184157444 27.05236351486044 23.227942394782694 50-51 23.087731963747096 26.84262966200742 27.148814840036483 22.920823534209 52-53 23.40871884207583 26.438712174979027 27.21092543604964 22.9416435468955 54-55 22.849550706828506 26.702566991361692 27.408294330815906 23.039587970993896 56-57 22.932071518370893 26.820232144451705 27.197579143164923 23.050117194012483 58-59 22.941522102881525 26.343537751472162 27.162993965098224 23.55194618054809 60-61 22.532068438132473 26.588728227675627 27.566941837635532 23.312261496556363 62-63 22.766208815266705 26.624864364167948 27.61260806349968 22.996318757065666 64-65 22.464380138799303 26.68570338268462 27.395769334462205 23.45414714405387 66-67 22.38035693947472 26.7480946814869 27.366684479116593 23.504863899921787 68-69 22.999460958736417 26.25512910752282 27.5496539933185 23.195755940422263 70-71 22.731985346437266 26.33356523851962 27.169766125655904 23.764683289387214 72-73 22.715675029054474 26.495406175825924 27.248310160306342 23.54060863481326 74-75 22.729708364579206 26.811936606352237 27.10368903785193 23.354665991216624 76-77 22.88089895218828 26.345415509705738 27.25557913543919 23.518106402666795 78-79 22.85420431246915 26.58839264531129 27.209512901811145 23.34789014040841 80-81 23.077857434479927 26.581993402615105 26.89847724731488 23.441671915590085 82-83 22.989591821004645 26.63734061529653 27.0329980014975 23.340069562201325 84-85 22.849493167507802 26.670926543956046 27.067150186825558 23.41243010171059 86-87 23.06178246624375 26.70125837400509 26.763954266409023 23.47300489334214 88-89 22.887650858111588 26.67457257908107 26.67758419134167 23.76019237146568 90-91 22.814921340765242 26.751523076639767 26.771332237490324 23.662223345104668 92-93 23.031798657886736 26.28257821895785 27.023051536123777 23.662571587031643 94-95 22.6836064414237 26.027986630877535 27.28788582035599 24.000521107342777 96-97 23.049901591539143 25.646435851382915 27.256613743865394 24.047048813212548 98-99 22.77589832363397 25.86480319648689 26.884027545952943 24.475270933926193 100-101 23.303755338525473 25.33047440659988 27.124411888018173 24.241358366856478 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 179.0 1 134.5 2 132.0 3 179.5 4 171.0 5 181.5 6 303.0 7 622.5 8 1021.0 9 1235.5 10 1118.5 11 1007.5 12 1099.5 13 1237.0 14 1421.0 15 1645.5 16 1813.0 17 2049.5 18 2354.0 19 2686.5 20 3178.5 21 3806.0 22 4666.0 23 5724.0 24 7129.5 25 9241.5 26 12324.0 27 16245.5 28 19799.0 29 23511.0 30 27978.5 31 34675.0 32 44305.5 33 48873.5 34 49537.0 35 53768.5 36 60228.5 37 66847.0 38 72286.5 39 76858.5 40 82412.0 41 87975.5 42 91472.5 43 94728.0 44 98708.0 45 104270.5 46 107822.0 47 110196.0 48 112226.0 49 110994.0 50 109530.0 51 107915.0 52 106482.0 53 106894.5 54 107091.5 55 101781.5 56 93831.0 57 86397.5 58 80183.5 59 71256.0 60 57941.0 61 47895.0 62 40787.5 63 31912.0 64 23115.5 65 17274.5 66 13029.0 67 9952.0 68 7635.0 69 5868.0 70 4560.0 71 3316.0 72 2429.0 73 1897.0 74 1445.0 75 1092.5 76 829.0 77 588.5 78 407.0 79 263.0 80 157.5 81 83.0 82 50.0 83 37.5 84 28.5 85 20.0 86 12.5 87 8.5 88 9.5 89 8.0 90 5.5 91 3.0 92 2.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18569881695711998 2 0.049650434105822525 3 0.048033268199395865 4 0.05412344618742815 5 0.060936187665565966 6 0.06613176323727711 7 0.036781922424895544 8 0.0331346971891248 9 0.040979672224556216 10-11 0.04307854712438655 12-13 0.03989582698939793 14-15 0.03476906698817301 16-17 0.03371962953825784 18-19 0.042132333030200746 20-21 0.05238585303265058 22-23 0.04971924967630876 24-25 0.06938329894275198 26-27 0.05467397075131807 28-29 0.046244063366753616 30-31 0.05584383544958416 32-33 0.051869736254003776 34-35 0.050785891018845494 36-37 0.048480569407556434 38-39 0.027078926986335634 40-41 0.025410149402044304 42-43 0.02196937087773228 44-45 0.003716040806256987 46-47 0.010649209532745717 48-49 0.010236316109828274 50-51 0.008068625639511698 52-53 0.008825596914860344 54-55 0.010666413425367277 56-57 0.008275072350970419 58-59 0.010545986177016357 60-61 0.010580393962259477 62-63 0.0034235746316904647 64-65 0.006210605236383206 66-67 0.003853671947229468 68-69 0.00803421785426858 70-71 0.023311274502213968 72-73 0.01961243758857854 74-75 0.02071348671635839 76-77 0.01231798711703705 78-79 0.00407732255130975 80-81 0.005763304028222642 82-83 0.004920313289766195 84-85 0.004541827652091873 86-87 0.007827771142809857 88-89 0.030915395040943545 90-91 0.03776254430432448 92-93 0.023311274502213968 94-95 0.03328953222271884 96-97 0.03719481584781299 98-99 0.021642496917922638 100-101 0.12379921130474666 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2906319.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.82956334900613 #Duplication Level Percentage of deduplicated Percentage of total 1 75.98485653049102 41.66216504703699 2 13.267698234196878 14.54924201654789 3 4.631505600543897 7.6183028917889475 4 2.133250009137053 4.678606660609919 5 1.0863932183934495 2.978323289491714 6 0.6569607216956055 2.161252170481079 7 0.43942431635407164 1.6865410373440495 8 0.31326272203445443 1.3740846610136175 9 0.2233918793016231 1.1023631278039685 >10 1.1466605959676024 11.297722132287419 >50 0.07332739040352997 2.787959330975716 >100 0.03796583809668766 3.8468779429929185 >500 0.0025252111354386454 1.003911465560035 >1k 0.0027146019703274513 3.053686632312159 >5k 6.313027837970816E-5 0.19896159375353134 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5748 0.1977759495774552 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4589 0.15789732648067883 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4006 0.13783758768393972 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3916 0.13474088701205889 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3587 0.12342072566707232 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3504 0.12056487949189336 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3235 0.11130918526149401 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2949 0.10146855868196161 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2949 0.10146855868196161 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2908 0.10005783948699369 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 6.88155704862405E-5 6 0.0 0.0 0.0 0.0 6.88155704862405E-5 7 0.0 0.0 0.0 0.0 6.88155704862405E-5 8 0.0 0.0 0.0 0.0 6.88155704862405E-5 9 0.0 0.0 0.0 0.0 6.88155704862405E-5 10-11 0.0 0.0 0.0 0.0 1.0322335572936075E-4 12-13 0.0 0.0 0.0 0.0 2.064467114587215E-4 14-15 0.0 0.0 0.0 3.440778524312025E-5 2.4085449670184175E-4 16-17 0.0 0.0 0.0 3.440778524312025E-5 2.75262281944962E-4 18-19 0.0 0.0 0.0 5.161167786468037E-5 3.440778524312025E-4 20-21 0.0 0.0 0.0 8.601946310780062E-5 4.3009731553900313E-4 22-23 0.0 0.0 0.0 2.7526228194496203E-4 4.817089934036835E-4 24-25 0.0 0.0 0.0 5.677284565114842E-4 4.817089934036835E-4 26-27 0.0 0.0 0.0 9.806218794289271E-4 4.817089934036835E-4 28-29 0.0 0.0 0.0 0.0028730500678005406 4.817089934036835E-4 30-31 0.0 0.0 0.0 0.010442762821286996 4.989128860252436E-4 32-33 0.0 0.0 0.0 0.03029605490656738 5.50524563889924E-4 34-35 0.0 0.0 0.0 0.0605749059205132 5.849323491330442E-4 36-37 0.0 0.0 0.0 0.1094167570731224 5.849323491330442E-4 38-39 0.0 0.0 0.0 0.18329027199010156 6.365440269977246E-4 40-41 0.0 0.0 0.0 0.26518080086872775 6.537479196192848E-4 42-43 0.0 0.0 0.0 0.3504432927011797 6.537479196192848E-4 44-45 0.0 0.0 0.0 0.45886222400225163 6.88155704862405E-4 46-47 0.0 0.0 0.0 0.5819388718168927 7.741751679702057E-4 48-49 0.0 0.0 0.0 0.7177808079567315 9.290102015642467E-4 50-51 0.0 0.0 0.0 0.8658891195357427 9.978257720504873E-4 52-53 0.0 0.0 0.0 1.006599757287483 0.001101049127779848 54-55 0.0 0.0 0.0 1.161864888197063 0.0011354569130229682 56-57 0.0 0.0 0.0 1.3451207524019215 0.0012214763761307689 58-59 0.0 0.0 0.0 1.5444106445300738 0.0013074958392385696 60-61 0.0 0.0 0.0 1.7553819797482657 0.0013419036244816896 62-63 0.0 0.0 0.0 1.9700177440948499 0.00137631140972481 64-65 0.0 0.0 0.0 2.1913630265638426 0.0015311464433188512 66-67 0.0 0.0 0.0 2.4443634714565055 0.0015827581211835316 68-69 0.0 0.0 0.0 2.7269029999803873 0.0016171659064266517 70-71 0.0 0.0 0.0 3.024495934548135 0.0016171659064266517 72-73 1.7203892621560123E-5 0.0 0.0 3.3361616532803176 0.0017547970473991326 74-75 3.440778524312025E-5 0.0 0.0 3.6412898928163084 0.0018924281883716137 76-77 3.440778524312025E-5 0.0 0.0 3.9807398981323106 0.0018924281883716137 78-79 3.440778524312025E-5 1.7203892621560123E-5 0.0 4.362803945471918 0.0018924281883716137 80-81 3.440778524312025E-5 3.440778524312025E-5 0.0 4.76277724503057 0.0019096320809931738 82-83 3.440778524312025E-5 3.440778524312025E-5 0.0 5.174242744860424 0.0019956515441009745 84-85 3.440778524312025E-5 3.440778524312025E-5 0.0 5.590921024154609 0.0019956515441009745 86-87 3.440778524312025E-5 3.440778524312025E-5 0.0 6.026403158084161 0.0021160787924518953 88-89 3.440778524312025E-5 3.440778524312025E-5 0.0 6.484301964099605 0.0022537099334243764 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4235 0.0 46.435818 1 GTATCAA 7275 0.0 39.074894 1 ATCAACG 8725 0.0 32.139156 3 TCAACGC 8850 0.0 31.631504 4 CAACGCA 9050 0.0 30.983383 5 TATCAAC 9130 0.0 30.765543 2 AACGCAG 9405 0.0 30.111376 6 ACGCAGA 10765 0.0 25.907312 7 CGCAGAG 10900 0.0 25.542854 8 TAAGGTG 1945 0.0 23.94594 5 TGGTATC 2155 0.0 23.37795 2 GCAGAGT 12185 0.0 21.9516 9 GTACATG 8620 0.0 21.653858 1 TACATGG 8345 0.0 21.585432 2 GTGTAGC 1735 0.0 21.406794 1 GTAAGGT 2060 0.0 20.995298 4 GTGGTAT 2480 0.0 20.928171 1 TAAGGTA 975 0.0 20.47352 4 GAGTACT 6870 0.0 20.467203 12-13 AGAGTAC 10435 0.0 19.89613 10-11 >>END_MODULE