FastQCFastQC Report
Thu 26 May 2016
SRR938470_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938470_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2906319
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55390.19058472246164307No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46730.16078758044110092No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA40230.13842252003307276No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA39500.135910751710325No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA38520.1325387887564992No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC36830.12672387305041188No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA36340.12503789157349898No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG32660.11237582660403073No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31100.10700821210610399No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40000.048.756931
GTATCAA68600.039.5935551
ATCAACG87300.030.7141153
TCAACGC88050.030.3979784
CAACGCA90250.029.6569755
TATCAAC92950.029.1189292
AACGCAG94150.028.5024786
TAAGGTG19600.025.4682355
CGCAGAG110350.024.2320738
ACGCAGA111700.023.9817287
GTAAGGT19950.022.8767324
GGTAAGG20900.022.064733
TACATGG86450.021.789272
GTACATG89850.021.3353351
AGGTAAG19450.021.0325222
GCAGAGT128500.020.4028349
AGAGTAC110550.020.27859110-11
ACATGGG89150.020.157823
GAGTACT72850.019.9175612-13
TGGTATC21450.019.514972
Produced by FastQC (version 0.11.5)