##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938470_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2906319 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80775991899031 31.0 31.0 34.0 30.0 34.0 2 32.03873284384818 31.0 31.0 34.0 30.0 34.0 3 32.108448866074234 33.0 31.0 34.0 30.0 34.0 4 35.77327574846395 37.0 35.0 37.0 35.0 37.0 5 35.636108080358696 37.0 35.0 37.0 33.0 37.0 6 35.660660099596775 37.0 35.0 37.0 33.0 37.0 7 35.595816563839 37.0 35.0 37.0 33.0 37.0 8 35.58109347253347 37.0 35.0 37.0 33.0 37.0 9 37.17162706502624 39.0 37.0 39.0 33.0 39.0 10-11 37.081220953377795 39.0 37.0 39.0 33.0 39.0 12-13 36.97906217452386 39.0 37.0 39.0 33.0 39.0 14-15 38.064893082968524 40.0 37.0 41.0 33.0 41.0 16-17 37.984266868158656 40.0 37.0 41.0 33.0 41.0 18-19 38.07736590511915 40.0 37.0 41.0 33.0 41.0 20-21 38.109295641669064 40.0 37.0 41.0 33.0 41.0 22-23 38.022045412083116 40.0 37.0 41.0 33.0 41.0 24-25 37.9288792799414 40.0 37.0 41.0 33.0 41.0 26-27 37.74576173503321 40.0 37.0 41.0 32.0 41.0 28-29 37.63310651721301 40.0 37.0 41.0 32.0 41.0 30-31 37.40706388390262 39.0 36.0 41.0 31.5 41.0 32-33 37.206558364721836 39.0 36.0 41.0 31.0 41.0 34-35 37.07373984067131 39.0 36.0 41.0 31.0 41.0 36-37 37.09172719856285 39.0 36.0 41.0 31.0 41.0 38-39 36.97305251075329 39.0 36.0 41.0 31.0 41.0 40-41 36.81201581794703 39.0 35.5 41.0 30.0 41.0 42-43 36.61041492692303 39.0 35.0 41.0 30.0 41.0 44-45 36.37349444434696 39.0 35.0 40.0 30.0 41.0 46-47 36.05777548851313 38.5 35.0 40.0 29.5 41.0 48-49 35.73092372172498 38.0 35.0 40.0 28.0 41.0 50-51 35.378321856616566 38.0 34.0 40.0 27.0 41.0 52-53 34.90208662572829 38.0 34.0 40.0 26.0 41.0 54-55 34.589266181723346 38.0 33.0 40.0 26.0 41.0 56-57 34.257061767823835 37.0 33.0 40.0 25.0 41.0 58-59 33.822269165910555 37.0 32.5 40.0 23.5 41.0 60-61 33.45801458821279 36.0 32.0 39.0 23.0 41.0 62-63 33.00415112725065 36.0 31.5 39.0 22.0 40.0 64-65 32.51059157649246 35.0 31.0 39.0 21.5 40.0 66-67 32.02919930675194 35.0 31.0 38.0 20.0 40.0 68-69 31.49813251745593 35.0 30.0 38.0 18.5 40.0 70-71 30.926139043924636 34.0 29.5 37.0 16.5 39.0 72-73 30.484632450876866 34.0 29.0 36.5 13.0 39.0 74-75 29.979496916890405 34.0 29.0 36.0 9.5 39.0 76-77 28.719844930993467 32.5 27.0 34.5 8.0 36.5 78-79 29.423725681867683 33.5 29.0 35.0 7.0 37.0 80-81 29.164715745243385 34.0 29.0 35.0 6.0 37.0 82-83 28.703972963738668 33.0 28.0 35.0 2.0 36.0 84-85 28.202096019053656 33.0 27.0 35.0 2.0 36.0 86-87 27.660240668694662 33.0 26.0 35.0 2.0 35.5 88-89 27.042908744704214 32.0 25.5 34.5 2.0 35.0 90-91 26.522108722407964 32.0 25.0 34.0 2.0 35.0 92-93 25.816255545244687 32.0 23.5 34.0 2.0 35.0 94-95 25.0860791606152 31.0 19.5 34.0 2.0 35.0 96-97 24.125386442438014 31.0 12.5 34.0 2.0 35.0 98-99 22.927649717735733 31.0 2.0 34.0 2.0 35.0 100-101 19.28162118473574 25.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3078654801360692 1101 2 0.32336391013706844 1101 3 0.28257087307720496 1101 4 0.1863656806792946 1101 5 0.1622481313016877 1101 6 0.21727787235158047 1101 7 0.2894377609511807 1101 8 0.26223928152762 1101 9 0.2552449753210553 1101 10-11 0.3540346989491425 1101 12-13 0.3938197152605838 1101 14-15 0.4260819458613483 1101 16-17 0.46010516538567714 1101 18-19 0.38786029982012593 1101 20-21 0.4762961249429267 1101 22-23 0.5510663781487963 1101 24-25 0.5288418267397574 1101 26-27 0.6086487473677593 1101 28-29 0.43756212115879833 1101 30-31 0.45811434390470396 1101 32-33 0.4359812932569582 1101 34-35 0.38112440640978207 1101 36-37 0.4424623877192957 1101 38-39 0.5296434490154454 1101 40-41 0.45174817056769 1101 42-43 0.524256401142047 1101 44-45 0.4132085395454297 1101 46-47 0.4872001649442339 1101 48-49 0.5410339044801376 1101 50-51 0.502915650843164 1101 52-53 0.5226211381463202 1101 54-55 0.5561104341296428 1101 56-57 0.5367628096343964 1101 58-59 0.5498404581473437 1101 60-61 0.6564223643305951 1101 62-63 0.564970397457472 1101 64-65 0.5647288270899438 1101 66-67 0.5354282099909184 1101 68-69 0.5847912709489869 1101 70-71 0.493483800845258 1101 72-73 0.4676485349821533 1101 74-75 0.46919469331382757 1101 76-77 0.49398096636716815 1101 78-79 0.582596891600712 1101 80-81 0.7921383026854478 1101 82-83 0.6820138947872856 1101 84-85 0.8149649428016232 1101 86-87 0.6927785045649699 1101 88-89 0.5445380998100298 1101 90-91 0.6602203024591269 1101 92-93 0.46311187634904627 1101 94-95 0.5759836267442857 1101 96-97 0.6673218561098757 1101 98-99 0.6057758427469615 1101 100-101 0.7837188976635865 1102 1 0.3804123187609001 1102 2 0.33118064424004245 1102 3 0.30508204912043624 1102 4 0.2545020257552082 1102 5 0.1602591137859264 1102 6 0.23775217142939198 1102 7 0.28477086802377016 1102 8 0.34787322700231726 1102 9 0.30263113346671133 1102 10-11 0.4609702756223655 1102 12-13 0.4596239396193269 1102 14-15 0.5334960507438069 1102 16-17 0.4755072908225131 1102 18-19 0.47466090223935 1102 20-21 0.4019671515480141 1102 22-23 0.40654834004154594 1102 24-25 0.5483834631352167 1102 26-27 0.4944162618051351 1102 28-29 0.3629162943437194 1102 30-31 0.3991936606679758 1102 32-33 0.46267645905481913 1102 34-35 0.44145800400859514 1102 36-37 0.43513392606254087 1102 38-39 0.5134807427955721 1102 40-41 0.5753607768262654 1102 42-43 0.5295503058898134 1102 44-45 0.5744482186087012 1102 46-47 0.5102416395986111 1102 48-49 0.6479185724866952 1102 50-51 0.5754253685652202 1102 52-53 0.5796502462370938 1102 54-55 0.533279753173197 1102 56-57 0.5361499833018897 1102 58-59 0.6882875714668515 1102 60-61 0.6979053709116059 1102 62-63 0.6296926137874905 1102 64-65 0.6992269392268895 1102 66-67 0.6676932346763138 1102 68-69 0.5702667897136031 1102 70-71 0.5794926011507116 1102 72-73 0.42224701938872045 1102 74-75 0.5069740281072512 1102 76-77 0.5594602502519237 1102 78-79 0.47523541885540865 1102 80-81 0.4505434030974911 1102 82-83 0.3439243353063439 1102 84-85 0.2819860036109674 1102 86-87 0.24638426997146823 1102 88-89 0.3369262598329392 1102 90-91 0.14882597506337092 1102 92-93 0.1808758855126058 1102 94-95 0.13751980901852434 1102 96-97 0.12195103154856568 1102 98-99 0.14117765000462867 1102 100-101 0.06272304990171662 1103 1 0.25914813408463644 1103 2 0.29206286807477966 1103 3 0.32111119721440673 1103 4 0.19262900220356016 1103 5 0.27118862520725173 1103 6 0.08182637774660151 1103 7 0.26602975444269816 1103 8 0.27897796484735693 1103 9 0.38075163426720593 1103 10-11 0.3169737514839497 1103 12-13 0.36755750407953514 1103 14-15 0.4980918538585186 1103 16-17 0.4281529641043207 1103 18-19 0.4206481121949537 1103 20-21 0.34419343064843133 1103 22-23 0.4393570307193002 1103 24-25 0.538416980371828 1103 26-27 0.5037772203034052 1103 28-29 0.4336096365842721 1103 30-31 0.35713848712445184 1103 32-33 0.45677363728486853 1103 34-35 0.36803260385578795 1103 36-37 0.3806541471048135 1103 38-39 0.4567478607099531 1103 40-41 0.4987328104740456 1103 42-43 0.5211146227665537 1103 44-45 0.722499829266134 1103 46-47 0.5884945968324615 1103 48-49 0.6370299497904455 1103 50-51 0.645304455678037 1103 52-53 0.6580114082191528 1103 54-55 0.6710859546938153 1103 56-57 0.6820827389460007 1103 58-59 0.6840958692545129 1103 60-61 0.5414585179356806 1103 62-63 0.6147808348911852 1103 64-65 0.5296104613906252 1103 66-67 0.44930565819392854 1103 68-69 0.43604030329006704 1103 70-71 0.17924253364272502 1103 72-73 0.37790322903590123 1103 74-75 0.4359997118576011 1103 76-77 0.22261490177632481 1103 78-79 0.2987104101849489 1103 80-81 0.39586174005897945 1103 82-83 0.22315094330824792 1103 84-85 0.23671181879392833 1103 86-87 0.40155467805246303 1103 88-89 0.4511573278413188 1103 90-91 0.45124839206694034 1103 92-93 0.3017531950216963 1103 94-95 0.3298756650646162 1103 96-97 0.12108008250782021 1103 98-99 0.06427728411772904 1103 100-101 -0.21041133002940882 1104 1 0.2894226242978597 1104 2 0.20066073709624987 1104 3 0.23184077941821357 1104 4 0.16287660417928151 1104 5 0.13797112189519822 1104 6 0.16336459063385433 1104 7 0.16657755942706132 1104 8 0.18379556317431422 1104 9 0.16183509800114848 1104 10-11 0.21581018627016846 1104 12-13 0.3057498210962635 1104 14-15 0.4262549562419693 1104 16-17 0.4820594723801719 1104 18-19 0.4106611400113067 1104 20-21 0.3351507302661716 1104 22-23 0.3842706724592446 1104 24-25 0.3595374705311727 1104 26-27 0.41091043952089734 1104 28-29 0.48819239137970527 1104 30-31 0.5493156752221182 1104 32-33 0.597844807853761 1104 34-35 0.5694600313273384 1104 36-37 0.4873689824082348 1104 38-39 0.49509971950017473 1104 40-41 0.48444053680174193 1104 42-43 0.5667168776562761 1104 44-45 0.4360327183803179 1104 46-47 0.46407258374097893 1104 48-49 0.518433599048322 1104 50-51 0.6996824876451626 1104 52-53 0.7266896725809744 1104 54-55 0.5813882147260969 1104 56-57 0.6726367150012678 1104 58-59 0.6340461578500296 1104 60-61 0.5371058314383319 1104 62-63 0.6946816324776179 1104 64-65 0.6283521261594416 1104 66-67 0.5497176751300898 1104 68-69 0.5130485338334374 1104 70-71 0.3870208991415396 1104 72-73 0.3428781162057071 1104 74-75 0.3150704759194376 1104 76-77 0.33463967421808505 1104 78-79 0.28856879390312073 1104 80-81 0.3669947192903855 1104 82-83 0.44295604031895053 1104 84-85 0.547596045095915 1104 86-87 0.5743237257239855 1104 88-89 0.4339744461960926 1104 90-91 0.3084307104795023 1104 92-93 0.48976980793637637 1104 94-95 0.5704042956501105 1104 96-97 0.5601400227072055 1104 98-99 0.6541956643585465 1104 100-101 0.49477126755065015 1105 1 0.17027420693644046 1105 2 0.1988087132231371 1105 3 0.12703248420908864 1105 4 0.15465919167144193 1105 5 -8.110708081758844E-4 1105 6 0.11607237726212816 1105 7 0.06356439205983833 1105 8 0.15900166614451905 1105 9 0.19691672638877833 1105 10-11 0.18132538563850886 1105 12-13 0.20298049386280326 1105 14-15 0.30494100626403764 1105 16-17 0.21725547340197693 1105 18-19 0.2909931109362702 1105 20-21 0.1779835720487526 1105 22-23 0.27705242392809026 1105 24-25 0.3189218322505951 1105 26-27 0.26084889891571805 1105 28-29 0.21517214103716498 1105 30-31 0.43285356333433356 1105 32-33 0.4200975423466886 1105 34-35 0.3078044729334337 1105 36-37 0.25000977105689515 1105 38-39 0.3218269204476698 1105 40-41 0.40572778047931735 1105 42-43 0.48915016996253513 1105 44-45 0.3407986318519036 1105 46-47 0.0925043433378292 1105 48-49 0.10134679196070095 1105 50-51 0.06082714290165825 1105 52-53 0.07942997122707851 1105 54-55 0.20966147823599357 1105 56-57 0.2056091262318489 1105 58-59 0.2893804510619873 1105 60-61 0.06756901157812223 1105 62-63 0.2982521487220069 1105 64-65 0.06147374432901387 1105 66-67 -0.011881567804358895 1105 68-69 0.012803532556180386 1105 70-71 -0.05411086742217108 1105 72-73 0.21847958853565785 1105 74-75 0.23592229078671778 1105 76-77 0.15285685449722308 1105 78-79 0.21151647277727292 1105 80-81 0.35262266569200307 1105 82-83 0.35670514043274437 1105 84-85 0.3949706996557225 1105 86-87 0.4528948225401308 1105 88-89 0.2928078453275553 1105 90-91 0.3005854444615288 1105 92-93 0.3006241773193459 1105 94-95 0.25002390504144145 1105 96-97 0.13253151384767747 1105 98-99 0.14201991367927747 1105 100-101 -0.29118121845757017 1106 1 0.13097982289694698 1106 2 0.17972992839396085 1106 3 0.21707722693840026 1106 4 0.1082830354209463 1106 5 0.011363983420288548 1106 6 0.00824024636131071 1106 7 0.11442728755361031 1106 8 0.12390253629313719 1106 9 0.06253520282405134 1106 10-11 0.10486495711467825 1106 12-13 0.11250079015305658 1106 14-15 0.20098907183230352 1106 16-17 0.08134563657467453 1106 18-19 0.29391243802738387 1106 20-21 0.2606041156253198 1106 22-23 0.18633365034126115 1106 24-25 0.22494158795620223 1106 26-27 0.23425270091440353 1106 28-29 0.27976571657962523 1106 30-31 0.40673601507270263 1106 32-33 0.3565589568425196 1106 34-35 0.1672870302954692 1106 36-37 -0.15247289510205064 1106 38-39 0.13731121402706492 1106 40-41 0.20921052823594977 1106 42-43 0.4110785271971906 1106 44-45 0.26374622347363896 1106 46-47 1.0992733923842479E-4 1106 48-49 0.1412047099958329 1106 50-51 0.2625478340340308 1106 52-53 0.14045676473281077 1106 54-55 -0.05982512501113746 1106 56-57 0.23556516548033102 1106 58-59 0.1537303101926497 1106 60-61 -0.17507886722211197 1106 62-63 -0.017945671259901985 1106 64-65 -0.3809320046810498 1106 66-67 -0.1435828908201202 1106 68-69 -0.7228415084579858 1106 70-71 -0.6327496707468256 1106 72-73 -0.3347073109563965 1106 74-75 -0.17675538250069422 1106 76-77 -0.07245293337146563 1106 78-79 -0.14313119522895335 1106 80-81 -0.8749555944187435 1106 82-83 -0.34510904804430353 1106 84-85 -0.18266201557516126 1106 86-87 -0.048280977400484204 1106 88-89 0.13375293915355968 1106 90-91 0.10986077770873237 1106 92-93 -0.16737842474008957 1106 94-95 -0.01886765843815752 1106 96-97 0.01541073341117638 1106 98-99 0.018418917280911273 1106 100-101 -0.31840118541051154 1107 1 0.07848922595866625 1107 2 0.13572868853145792 1107 3 0.11922986881706521 1107 4 0.11355476424266442 1107 5 0.013362567756381338 1107 6 0.04771691335182027 1107 7 0.012666035107422147 1107 8 0.004177156585249975 1107 9 0.04586645429870373 1107 10-11 0.11883769864498817 1107 12-13 0.11972946613719415 1107 14-15 0.16825415721164205 1107 16-17 0.15547252817460588 1107 18-19 0.17477113533900734 1107 20-21 0.1111127830969707 1107 22-23 0.16938038112918008 1107 24-25 0.1930625485720654 1107 26-27 0.20682883911280925 1107 28-29 0.22524877718947067 1107 30-31 0.13159063194131448 1107 32-33 0.18895198400383606 1107 34-35 0.2673214966225643 1107 36-37 0.16339942681806718 1107 38-39 0.17829491538675057 1107 40-41 0.11198541454333366 1107 42-43 0.21133459581525216 1107 44-45 0.21117813512880446 1107 46-47 0.19810856719156789 1107 48-49 0.2168039383202114 1107 50-51 0.11886835699693421 1107 52-53 0.06861991768465714 1107 54-55 -5.869927622157434E-4 1107 56-57 -0.16943268326568983 1107 58-59 -0.019031576063845534 1107 60-61 -0.13916680493290556 1107 62-63 -0.02545077181367361 1107 64-65 0.009466231753329168 1107 66-67 0.0620226293438364 1107 68-69 0.026777974038207475 1107 70-71 -0.15517591512002227 1107 72-73 -0.21748478802125248 1107 74-75 -0.18527249769224952 1107 76-77 -0.1513842921774966 1107 78-79 -0.11042519531222794 1107 80-81 0.052589940457014706 1107 82-83 -0.043968189485781295 1107 84-85 -0.08925595597848712 1107 86-87 -0.02293666110600867 1107 88-89 -0.0814892735383772 1107 90-91 -0.128916847399406 1107 92-93 -0.31880125949140137 1107 94-95 -0.5027714210507526 1107 96-97 -0.3664241243053503 1107 98-99 -0.39838075702386533 1107 100-101 -0.8240638410294459 1108 1 0.15868486102487722 1108 2 0.1718804433289165 1108 3 0.1564414768839697 1108 4 0.1199420045892623 1108 5 0.16443820698104616 1108 6 0.03263631976582104 1108 7 0.0647398750314494 1108 8 0.08136302140208329 1108 9 0.194752724267083 1108 10-11 0.1408245943848243 1108 12-13 0.20690213125381973 1108 14-15 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-0.6532371879866936 2316 68-69 -0.5871546430326653 2316 70-71 -0.7120159520136689 2316 72-73 -0.5463622805170942 2316 74-75 -0.31977991507173087 2316 76-77 -0.34853418641051803 2316 78-79 -0.3131156132639923 2316 80-81 -0.5157808878660006 2316 82-83 -0.32033542491193856 2316 84-85 -0.2873390913739158 2316 86-87 -0.23083407292701708 2316 88-89 -0.09539458265535572 2316 90-91 0.06242765807808226 2316 92-93 0.08628122148202522 2316 94-95 0.23133836176834421 2316 96-97 0.23828702957733938 2316 98-99 0.4376788431910583 2316 100-101 0.53769398660123 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 19.0 4 32.0 5 93.0 6 204.0 7 479.0 8 1119.0 9 2091.0 10 3194.0 11 5090.0 12 7339.0 13 10201.0 14 13268.0 15 17375.0 16 21054.0 17 25628.0 18 24972.0 19 22812.0 20 23163.0 21 24479.0 22 27147.0 23 30580.0 24 35739.0 25 42236.0 26 50286.0 27 60204.0 28 72848.0 29 87242.0 30 104781.0 31 125412.0 32 151160.0 33 186685.0 34 238195.0 35 313189.0 36 418598.0 37 486638.0 38 259034.0 39 13725.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.22804327689976 18.95070997292785 14.91717326587547 23.904073484296916 2 15.986154524048853 20.02185946600939 39.06944081076243 24.922545199179325 3 19.50196332646548 23.114711116432897 29.732318707136102 27.65100684996552 4 13.59461917291254 16.673049310829267 35.963602068458414 33.76872944779978 5 14.208326431317394 36.88972260267015 34.21851329026935 14.68343767574312 6 33.57852321097581 35.74734913820541 16.596285541951865 14.07784210886692 7 29.565956111493612 31.903104924132553 19.97330644020839 18.55763252416545 8 26.862708463867868 35.099106464225024 19.19823666982186 18.83994840208525 9 26.16402397672107 16.609635762626198 19.607758129785477 37.61858213086725 10-11 25.32079238376792 26.198724228138754 27.138555678161964 21.341927709931362 12-13 26.167722813634704 23.223001329172742 27.371806054325077 23.237469802867476 14-15 22.622172583257377 25.004464410135295 25.326452464440415 27.046910542166913 16-17 22.743098744494326 28.05674463126725 26.32899210306921 22.87116452116922 18-19 22.851620899151126 26.618447596426957 28.171064497737515 22.3588670066844 20-21 23.07363713343236 25.989077592652425 28.46031354438381 22.47697172953141 22-23 22.94562296843533 25.854697987385418 28.372178002483555 22.827501041695697 24-25 22.903246895123207 26.148473238448 28.318059122978756 22.63022074345004 26-27 22.44692653229486 26.491208389623633 28.065105885413477 22.996759192668026 28-29 22.57224688686961 26.855310790040598 27.963791999432956 22.608650323656832 30-31 22.72906036811513 26.349309900255268 27.866108297127738 23.055521434501856 32-33 22.324992542112547 26.29830035863235 27.990423625211136 23.38628347404397 34-35 23.12723921678879 26.511122378945295 27.737305147383328 22.62433325688259 36-37 23.33874396035533 25.949315585788547 27.75479623268965 22.957144221166477 38-39 22.709975048142823 26.279761193023255 27.8058107908395 23.20445296799442 40-41 23.165103204987478 26.09589705721437 27.91377825981932 22.82522147797883 42-43 22.91248503040885 26.153262807441124 27.67440208085274 23.259850081297287 44-45 22.805634894173696 26.415304032351578 27.893600117536995 22.885460955937734 46-47 23.34370383980561 26.662678804356986 27.158649824743947 22.834967531093454 48-49 23.00846878818189 26.74801011175993 27.08226454150422 23.161256558553962 50-51 23.13560555465522 26.7672956753887 27.214786126368097 22.882312643587987 52-53 23.4897992959479 26.38431982174015 27.25096591255124 22.874914969760717 54-55 22.885237305333654 26.703090748125035 27.408553568964727 23.003118377576584 56-57 22.973218012200313 26.775020223175776 27.216059214422096 23.03570255020182 58-59 22.892738202516654 26.47092421719708 27.14769094514401 23.488646635142256 60-61 22.52044596618609 26.66679053469354 27.549229798931226 23.26353370018914 62-63 22.760749938324047 26.69706938570749 27.567104643365028 22.975076032603443 64-65 22.408534644682845 26.75019500612287 27.363995487074888 23.4772748621194 66-67 22.34566979445119 26.79499517774558 27.31945620522469 23.53987882257854 68-69 22.971269075198357 26.321601723982795 27.407692994530663 23.29943620628818 70-71 22.677971012099167 26.436474905000008 27.10030499092322 23.785249091977608 72-73 22.685431296426856 26.606164017095164 27.08589456284737 23.62251012363061 74-75 22.659453418568297 26.887464865350292 26.96128676858941 23.491794947492 76-77 22.67115206555096 26.440370103901188 27.216781777912196 23.671696052635653 78-79 22.76565304772119 26.606783357229542 27.175320396694236 23.45224319835503 80-81 22.884755928876874 26.67318472661309 26.886119869909482 23.555939474600553 82-83 22.81119298104964 26.752316129406307 26.97113360665877 23.46535728288529 84-85 22.628349438394594 26.757312655541515 27.028700070797463 23.58563783526643 86-87 22.827181858987228 26.874883118460545 26.63696520727968 23.660969815272544 88-89 22.59890183999262 26.885600189380643 26.506426004605142 24.00907196602159 90-91 22.48823649842494 26.86523973457067 26.726675578540622 23.919848188463767 92-93 22.63151031227753 26.50765081153208 26.956091488366795 23.904747387823594 94-95 22.285675945590942 26.285737879636343 27.16895177488351 24.259634399889208 96-97 22.53221730515771 25.882951231945157 27.189019372790696 24.395812090106435 98-99 22.243941210777223 26.201943010306866 26.832500950516376 24.721614828399538 100-101 22.390680856210356 25.948153996198283 27.11481897114855 24.54634617644281 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 177.0 1 126.5 2 121.0 3 170.0 4 146.0 5 153.5 6 288.0 7 631.0 8 998.5 9 1146.0 10 1068.0 11 954.0 12 1028.5 13 1146.5 14 1278.5 15 1499.5 16 1697.5 17 1900.5 18 2183.5 19 2500.5 20 2921.5 21 3521.5 22 4290.0 23 5364.5 24 6910.5 25 8902.5 26 11957.5 27 15876.0 28 19364.0 29 23282.5 30 28138.5 31 34724.5 32 45013.0 33 49629.0 34 49462.5 35 53558.0 36 60082.0 37 66854.0 38 72425.5 39 77017.5 40 82519.0 41 88401.0 42 91750.0 43 95399.0 44 99832.5 45 106034.0 46 109605.0 47 111921.0 48 113875.0 49 111961.5 50 110655.0 51 109396.0 52 108109.0 53 108819.5 54 109667.0 55 103189.0 56 94255.0 57 86719.0 58 80013.0 59 70487.0 60 56671.0 61 46683.0 62 39617.0 63 30294.0 64 21151.5 65 15314.5 66 11233.5 67 8653.5 68 6649.5 69 5098.5 70 3913.0 71 2712.0 72 1905.0 73 1562.0 74 1180.0 75 852.5 76 652.5 77 441.5 78 287.5 79 184.5 80 108.5 81 53.0 82 30.0 83 17.0 84 12.0 85 6.0 86 3.0 87 1.0 88 1.0 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.7848563767432276E-4 2 0.04813649155512523 3 9.290102015642467E-4 4 0.0 5 0.08870327035676401 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.37631140972481E-4 26-27 0.008705169666509423 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010614801747502596 36-37 5.161167786468038E-5 38-39 0.00421495369228223 40-41 0.00553965342414236 42-43 3.612817450527626E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 6.88155704862405E-5 68-69 8.773985236995663E-4 70-71 1.0322335572936076E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0012042724835092087 82-83 5.333206712683638E-4 84-85 0.004868701611901515 86-87 0.002219302148181256 88-89 1.0322335572936076E-4 90-91 0.002890253960422101 92-93 8.946024163211264E-4 94-95 5.161167786468038E-5 96-97 1.7203892621560123E-5 98-99 1.37631140972481E-4 100-101 1.7203892621560123E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2906319.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.04050763197735 #Duplication Level Percentage of deduplicated Percentage of total 1 73.62034265900715 38.31240004014849 2 14.538809580053655 15.132140618212949 3 5.022889654509271 7.841811822002093 4 2.3315499319511472 4.853401681121597 5 1.242823925089673 3.233859397941658 6 0.7424018380103165 2.318098111018193 7 0.49977712955585313 1.8206058867457378 8 0.3396618446292469 1.4140939854175862 9 0.2493742924714895 1.167980829352335 >10 1.2738366926033546 11.992684148369047 >50 0.08910333486020462 3.181872859387737 >100 0.04367636233916413 4.271789219437312 >500 0.0024465739313592077 0.8935521869312381 >1k 0.0032400573683569224 3.3751063116463538 >5k 6.612361976238618E-5 0.19060290226758378 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5539 0.19058472246164307 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4673 0.16078758044110092 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4023 0.13842252003307276 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3950 0.135910751710325 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3852 0.1325387887564992 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3683 0.12672387305041188 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3634 0.12503789157349898 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3266 0.11237582660403073 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3110 0.10700821210610399 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.440778524312025E-5 2 0.0 0.0 0.0 0.0 3.440778524312025E-5 3 0.0 0.0 0.0 0.0 3.440778524312025E-5 4 0.0 0.0 0.0 0.0 3.440778524312025E-5 5 0.0 0.0 0.0 0.0 3.440778524312025E-5 6 0.0 0.0 0.0 0.0 1.0322335572936075E-4 7 0.0 0.0 0.0 0.0 1.0322335572936075E-4 8 0.0 0.0 0.0 0.0 1.0322335572936075E-4 9 0.0 0.0 0.0 0.0 1.0322335572936075E-4 10-11 0.0 0.0 0.0 0.0 1.37631140972481E-4 12-13 0.0 0.0 0.0 0.0 3.2687395980964234E-4 14-15 0.0 0.0 0.0 3.440778524312025E-5 4.817089934036835E-4 16-17 0.0 0.0 0.0 3.440778524312025E-5 6.021362417546044E-4 18-19 0.0 0.0 0.0 5.161167786468037E-5 6.88155704862405E-4 20-21 0.0 0.0 0.0 8.601946310780062E-5 8.429907384564461E-4 22-23 0.0 0.0 0.0 2.580583893234019E-4 8.601946310780063E-4 24-25 0.0 0.0 0.0 5.333206712683639E-4 8.601946310780063E-4 26-27 0.0 0.0 0.0 9.462140941858068E-4 9.290102015642467E-4 28-29 0.0 0.0 0.0 0.0029074578530436613 9.63417986807367E-4 30-31 0.0 0.0 0.0 0.010459966713908557 9.978257720504873E-4 32-33 0.0 0.0 0.0 0.030622928866377023 0.001101049127779848 34-35 0.0 0.0 0.0 0.061194246054889365 0.001101049127779848 36-37 0.0 0.0 0.0 0.11060382566401004 0.001101049127779848 38-39 0.0 0.0 0.0 0.1847353989703126 0.0011354569130229682 40-41 0.0 0.0 0.0 0.26722806409069344 0.0011354569130229682 42-43 0.0 0.0 0.0 0.3526797987419825 0.0011870685908876486 44-45 0.0 0.0 0.0 0.4617180701774306 0.0012214763761307689 46-47 0.0 0.0 0.0 0.5855000775895558 0.0012730880539954493 48-49 0.0 0.0 0.0 0.7220129655416353 0.0013246997318601297 50-51 0.0 0.0 0.0 0.8703965394025914 0.0014795347654541708 52-53 0.0 0.0 0.0 1.0115544783624921 0.0016859814769128922 54-55 0.0 0.0 0.0 1.1671120754466389 0.001789204832642253 56-57 0.0 0.0 0.0 1.3514173771014124 0.001789204832642253 58-59 0.0 0.0 0.0 1.5510857548672392 0.001789204832642253 60-61 0.0 0.0 0.0 1.7635022170656423 0.001789204832642253 62-63 0.0 0.0 0.0 1.97806916584174 0.001789204832642253 64-65 0.0 0.0 0.0 2.199758526163164 0.0018064087252638132 66-67 0.0 0.0 0.0 2.4542557097139026 0.0018408165105069333 68-69 0.0 0.0 0.0 2.738808093674507 0.0018580204031284934 70-71 0.0 0.0 0.0 3.038431087571598 0.001926835973614734 72-73 0.0 0.0 0.0 3.35415692496247 0.002081671007208775 74-75 0.0 0.0 0.0 3.664601167318522 0.002236506040802816 76-77 0.0 0.0 0.0 4.009023097602156 0.002339729396532177 78-79 0.0 0.0 0.0 4.399689091252544 0.002356933289153737 80-81 0.0 0.0 0.0 4.808814861685864 0.002374137181775297 82-83 0.0 0.0 0.0 5.227592015879881 0.002374137181775297 84-85 0.0 0.0 0.0 5.654919504706813 0.002374137181775297 86-87 0.0 1.7203892621560123E-5 0.0 6.103115315283697 0.0025633800006124585 88-89 0.0 3.440778524312025E-5 0.0 6.575723449490575 0.00275262281944962 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4000 0.0 48.75693 1 GTATCAA 6860 0.0 39.593555 1 ATCAACG 8730 0.0 30.714115 3 TCAACGC 8805 0.0 30.397978 4 CAACGCA 9025 0.0 29.656975 5 TATCAAC 9295 0.0 29.118929 2 AACGCAG 9415 0.0 28.502478 6 TAAGGTG 1960 0.0 25.468235 5 CGCAGAG 11035 0.0 24.232073 8 ACGCAGA 11170 0.0 23.981728 7 GTAAGGT 1995 0.0 22.876732 4 GGTAAGG 2090 0.0 22.06473 3 TACATGG 8645 0.0 21.78927 2 GTACATG 8985 0.0 21.335335 1 AGGTAAG 1945 0.0 21.032522 2 GCAGAGT 12850 0.0 20.402834 9 AGAGTAC 11055 0.0 20.278591 10-11 ACATGGG 8915 0.0 20.15782 3 GAGTACT 7285 0.0 19.91756 12-13 TGGTATC 2145 0.0 19.51497 2 >>END_MODULE