FastQCFastQC Report
Thu 26 May 2016
SRR938469_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938469_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2918256
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58020.1988173758573614No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45740.15673744866797154No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA40180.13768497349101655No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA39660.13590308732338768No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA36110.12373828752515201No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA35610.12202493544089346No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC32470.11126508435174982No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31530.10804398243334376No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA30050.10297246026393847No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44300.048.1922041
GTATCAA74550.038.8010561
ATCAACG92700.030.8616073
TCAACGC94000.030.3336874
CAACGCA95850.029.8969555
TATCAAC95900.029.8813692
AACGCAG100800.028.4730176
ACGCAGA114000.025.003417
CGCAGAG114900.024.6835238
GTACATG85600.022.43541
TACATGG84900.022.3899972
TAAGGTG18800.021.739145
GTGTAGC17000.021.3042951
GCAGAGT128600.021.1300479
GAGTACT71500.020.9929912-13
TAAGGTA9750.020.9587614
ACATGGG88650.020.3707313
GTAAGGT18550.020.2388084
AGAGTAC107600.019.9093710-11
CAGAGTA119700.019.66268310-11
Produced by FastQC (version 0.11.5)