FastQCFastQC Report
Thu 26 May 2016
SRR938469_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938469_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2918256
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55230.18925687122719873No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46960.1609180277535624No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA40380.13837031432471997No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA39940.13686256449057246No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA39830.13648562703203557No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC37910.12990635502848277No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA36950.12661671902670638No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG32900.11273856714421215No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30430.10427460784797496No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42050.050.2238161
GTATCAA71450.038.4785161
ATCAACG91900.029.7475593
TCAACGC93250.029.3168984
TATCAAC94200.029.0341872
CAACGCA94500.028.9794225
AACGCAG98600.027.7935356
TAAGGTG19450.026.8888425
GTAAGGT19250.024.945354
ACGCAGA112950.024.1362937
CGCAGAG114750.023.6335148
GTACATG90750.022.69521
GTATAGG6550.022.5119511
TACATGG89650.022.3900432
GGTAAGG20050.022.2901253
AGGTAAG19450.021.7652242
ACATGGG88850.021.404333
GCAGAGT130850.020.580429
TGGTATC21350.020.496642
AGAGTAC110200.020.191710-11
Produced by FastQC (version 0.11.5)