##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938469_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2918256 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.816564071143862 31.0 31.0 34.0 30.0 34.0 2 32.05474228443289 33.0 31.0 34.0 30.0 34.0 3 32.12668388242841 33.0 31.0 34.0 30.0 34.0 4 35.792032296001445 37.0 35.0 37.0 35.0 37.0 5 35.656249143323954 37.0 35.0 37.0 33.0 37.0 6 35.69307284898926 37.0 35.0 37.0 33.0 37.0 7 35.62489685620453 37.0 35.0 37.0 33.0 37.0 8 35.601102165128765 37.0 35.0 37.0 33.0 37.0 9 37.180407065041585 39.0 37.0 39.0 33.0 39.0 10-11 37.11134218519554 39.0 37.0 39.0 33.0 39.0 12-13 37.012191185420335 39.0 37.0 39.0 33.0 39.0 14-15 38.08903314171204 40.0 37.0 41.0 33.0 41.0 16-17 38.010280112505555 40.0 37.0 41.0 33.0 41.0 18-19 37.99420287322291 40.0 37.0 41.0 33.0 41.0 20-21 37.97032474190064 40.0 37.0 41.0 33.0 41.0 22-23 38.008222376652355 40.0 37.0 41.0 33.0 41.0 24-25 37.938725046740245 40.0 37.0 41.0 33.0 41.0 26-27 37.77422165841516 40.0 37.0 41.0 32.0 41.0 28-29 37.64754788476405 40.0 37.0 41.0 32.0 41.0 30-31 37.41109278966616 39.0 36.0 41.0 31.5 41.0 32-33 37.20828124742997 39.0 36.0 41.0 31.0 41.0 34-35 37.064373550504136 39.0 36.0 41.0 31.0 41.0 36-37 37.090920570368056 39.0 36.0 41.0 31.0 41.0 38-39 36.944781917693305 39.0 36.0 41.0 31.0 41.0 40-41 36.776609899885415 39.0 35.5 41.0 30.0 41.0 42-43 36.56429919102368 39.0 35.0 40.5 30.0 41.0 44-45 36.31436652576059 39.0 35.0 40.0 30.0 41.0 46-47 36.00021211298803 38.5 35.0 40.0 29.5 41.0 48-49 35.63311803351043 38.0 34.5 40.0 28.0 41.0 50-51 35.2806014962361 38.0 34.0 40.0 27.0 41.0 52-53 34.771376637279246 38.0 33.5 40.0 26.0 41.0 54-55 34.45222917386275 38.0 33.0 40.0 25.5 41.0 56-57 34.09245573383555 37.0 33.0 40.0 24.0 41.0 58-59 33.647410816597315 37.0 32.5 39.5 23.0 41.0 60-61 33.24239545810923 36.0 32.0 39.0 22.5 40.5 62-63 32.76786615019381 36.0 31.0 39.0 21.5 40.0 64-65 32.27076514192038 35.0 31.0 38.5 20.0 40.0 66-67 31.784415589310875 35.0 30.5 38.0 18.5 40.0 68-69 31.230452023400275 34.5 30.0 37.5 17.0 40.0 70-71 30.668574484212492 34.0 29.0 37.0 12.5 39.0 72-73 30.0914054490079 34.0 29.0 36.0 8.0 39.0 74-75 29.48643573421934 34.0 28.0 36.0 7.0 38.5 76-77 28.218593913625124 31.5 26.5 34.5 6.5 36.5 78-79 28.848868125346097 33.0 28.0 35.0 2.0 37.0 80-81 28.56766455718758 33.0 27.5 35.0 2.0 37.0 82-83 28.092726443464862 33.0 26.5 35.0 2.0 36.0 84-85 27.523152526714586 33.0 26.0 35.0 2.0 36.0 86-87 26.90698674139623 32.0 25.0 35.0 2.0 35.0 88-89 26.17791962048566 32.0 24.0 34.0 2.0 35.0 90-91 25.663530889682058 32.0 22.5 34.0 2.0 35.0 92-93 24.92215847410234 31.0 19.5 34.0 2.0 35.0 94-95 24.160601228953183 31.0 12.5 34.0 2.0 35.0 96-97 23.212272672445458 31.0 2.0 34.0 2.0 35.0 98-99 22.067110459123533 30.0 2.0 34.0 2.0 35.0 100-101 18.891553551162062 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28348745927273455 1101 2 0.3508186065276888 1101 3 0.32562204664664307 1101 4 0.2885642872635543 1101 5 0.24071780708057844 1101 6 0.24736177747431043 1101 7 0.2639817876181709 1101 8 0.2785665972443425 1101 9 0.41593538979523714 1101 10-11 0.39900063856069323 1101 12-13 0.38212076715975485 1101 14-15 0.5424153347388412 1101 16-17 0.4973522769918475 1101 18-19 0.5855538221311463 1101 20-21 0.5250896018794577 1101 22-23 0.5303207469416762 1101 24-25 0.5424221989582847 1101 26-27 0.5436737859752512 1101 28-29 0.4453288833602116 1101 30-31 0.5659538819329129 1101 32-33 0.4467058589270323 1101 34-35 0.2543790519466782 1101 36-37 0.6906961798007103 1101 38-39 0.6727977947022197 1101 40-41 0.8941311856051044 1101 42-43 0.6752854195905442 1101 44-45 0.7528387568127357 1101 46-47 0.8703603723071822 1101 48-49 0.813199586605613 1101 50-51 0.9377603109975468 1101 52-53 0.8772438336881336 1101 54-55 1.014380229126509 1101 56-57 1.0280083599650922 1101 58-59 1.2395012267308942 1101 60-61 1.3646956483298354 1101 62-63 1.298339764793731 1101 64-65 1.4682936159292694 1101 66-67 1.5309351052260354 1101 68-69 1.539745247601921 1101 70-71 1.5300294256691487 1101 72-73 1.689855744094423 1101 74-75 1.6546552915802266 1101 76-77 1.5316680197988504 1101 78-79 1.8504469002622201 1101 80-81 2.288590756250141 1101 82-83 2.250751502589633 1101 84-85 2.424007414769008 1101 86-87 2.5368843170557476 1101 88-89 2.525846395902711 1101 90-91 2.700919431582232 1101 92-93 3.097341219379789 1101 94-95 3.1695354765153674 1101 96-97 3.494924115300517 1101 98-99 3.724652063077876 1101 100-101 4.076382809830456 1102 1 0.3749631342725408 1102 2 0.35863926635013854 1102 3 0.341611341358643 1102 4 0.2577283223909461 1102 5 0.22071482747755766 1102 6 0.27566667599488426 1102 7 0.26107295016881693 1102 8 0.30226481666549887 1102 9 0.26907687571262073 1102 10-11 0.30283022655076053 1102 12-13 0.3975489919814379 1102 14-15 0.537139575139598 1102 16-17 0.40803734625431787 1102 18-19 0.5511839682647164 1102 20-21 0.6192372092848331 1102 22-23 0.5206491417603516 1102 24-25 0.45985700018949416 1102 26-27 0.4937658344257301 1102 28-29 0.5012524405805578 1102 30-31 0.5240545446285552 1102 32-33 0.3290027604059276 1102 34-35 0.5083044128910004 1102 36-37 0.5772302363972059 1102 38-39 0.6254123963532408 1102 40-41 0.5485852930676813 1102 42-43 0.5748481894507975 1102 44-45 0.49581287949256847 1102 46-47 0.4623678532390585 1102 48-49 0.672106471350844 1102 50-51 0.7458202701876218 1102 52-53 0.7831405212740989 1102 54-55 0.825774949376374 1102 56-57 0.9094926545307729 1102 58-59 0.9760431313889129 1102 60-61 1.002116269843107 1102 62-63 1.0398159718654938 1102 64-65 0.9715203663795364 1102 66-67 0.9041092337586676 1102 68-69 1.0131620009519793 1102 70-71 1.2477567334648008 1102 72-73 1.2490515439183092 1102 74-75 1.2777670346745964 1102 76-77 1.2832719507465526 1102 78-79 1.6854932836360312 1102 80-81 1.8672502807374265 1102 82-83 1.8735511854747386 1102 84-85 2.110317336048965 1102 86-87 2.1029349330620697 1102 88-89 2.315200832983873 1102 90-91 2.3338420496955585 1102 92-93 2.7240165144802013 1102 94-95 2.805532938532078 1102 96-97 2.9523402674092516 1102 98-99 3.3219737458086804 1102 100-101 3.861082814353061 1103 1 0.25509936284630186 1103 2 0.26494686392909017 1103 3 0.27557982891999444 1103 4 0.2228065925026712 1103 5 0.1346256248439417 1103 6 0.23844781349836808 1103 7 0.21581655072170491 1103 8 0.16578815973309702 1103 9 0.28762818242875454 1103 10-11 0.40473875514420143 1103 12-13 0.44014354334947114 1103 14-15 0.5747126779165441 1103 16-17 0.5680158593393685 1103 18-19 0.5220686875168354 1103 20-21 0.49751626324739107 1103 22-23 0.4888909193746187 1103 24-25 0.3703021702324989 1103 26-27 0.4532668321237381 1103 28-29 0.4765803012656349 1103 30-31 0.5333557472315107 1103 32-33 0.5363820210618755 1103 34-35 0.6369857397143903 1103 36-37 0.7518775929445809 1103 38-39 0.7382560017604831 1103 40-41 0.7432733495621662 1103 42-43 0.7115832769952846 1103 44-45 0.817267857890684 1103 46-47 0.8214295316711429 1103 48-49 0.9316865439617956 1103 50-51 0.8932281715471007 1103 52-53 1.124118444171586 1103 54-55 1.2469091570048718 1103 56-57 1.0920292346621423 1103 58-59 1.202679624397362 1103 60-61 1.2910262368590324 1103 62-63 1.280568422676808 1103 64-65 1.2055109540377202 1103 66-67 1.2205616700069157 1103 68-69 1.2930109282669306 1103 70-71 1.2222478563796635 1103 72-73 1.291747014803665 1103 74-75 1.483579232395094 1103 76-77 1.3187825907295867 1103 78-79 1.377572506734598 1103 80-81 1.7373783591020668 1103 82-83 1.924605091826539 1103 84-85 1.9311400983822224 1103 86-87 2.130097485022848 1103 88-89 2.3023940184892737 1103 90-91 2.356931924274317 1103 92-93 2.552500856625471 1103 94-95 2.575560225125624 1103 96-97 3.094918978245456 1103 98-99 3.5470731962444226 1103 100-101 3.775334694138756 1104 1 0.27793075514281895 1104 2 0.3079287398708317 1104 3 0.29474828314331347 1104 4 0.24861233290437923 1104 5 0.20631913226763032 1104 6 0.16116969891690047 1104 7 0.19350613831075947 1104 8 0.2629593741631524 1104 9 0.28055652302803225 1104 10-11 0.29578647651192824 1104 12-13 0.32809821310522835 1104 14-15 0.5138546872642138 1104 16-17 0.45419484887957395 1104 18-19 0.49913534054082476 1104 20-21 0.5531396058748044 1104 22-23 0.46515320235549495 1104 24-25 0.6261431867096903 1104 26-27 0.4916864493247246 1104 28-29 0.5191423602385683 1104 30-31 0.5155931591174578 1104 32-33 0.47809153427396467 1104 34-35 0.5435958623401618 1104 36-37 0.548322030080449 1104 38-39 0.5436934628575258 1104 40-41 0.5827163505622295 1104 42-43 0.6411151137752213 1104 44-45 0.7120428328283523 1104 46-47 0.6587940945633051 1104 48-49 0.6493832939584507 1104 50-51 0.6076323457202903 1104 52-53 0.7508900133113556 1104 54-55 0.7009680373548974 1104 56-57 0.8561826636647751 1104 58-59 0.9330359298131441 1104 60-61 0.940722991728066 1104 62-63 1.0364410487589382 1104 64-65 1.0536770488080691 1104 66-67 1.1305400731554442 1104 68-69 1.2297507903540463 1104 70-71 1.1997121570156324 1104 72-73 1.2661479853829825 1104 74-75 1.458123633840728 1104 76-77 1.4187075559833815 1104 78-79 1.7196839918013396 1104 80-81 1.804492493528766 1104 82-83 1.8735086222357076 1104 84-85 2.0969450226592485 1104 86-87 2.3350549551825246 1104 88-89 2.333974328202501 1104 90-91 2.4197961808151867 1104 92-93 2.659823383006394 1104 94-95 2.9373230178798977 1104 96-97 3.311177887098946 1104 98-99 3.68530784548744 1104 100-101 4.185543317531327 1105 1 0.23611191182529723 1105 2 0.27081139709671476 1105 3 0.23847230816326004 1105 4 0.18498488466776308 1105 5 0.1356246236874341 1105 6 0.148266051808136 1105 7 0.29286244856921684 1105 8 0.26543559729027066 1105 9 0.3842138935087931 1105 10-11 0.3213698203402018 1105 12-13 0.4396677782415068 1105 14-15 0.548899454019967 1105 16-17 0.5269220527006624 1105 18-19 0.5059487731329781 1105 20-21 0.32648787700811965 1105 22-23 0.4419962306825127 1105 24-25 0.3545415070526161 1105 26-27 0.5049148565657688 1105 28-29 0.4858895464281474 1105 30-31 0.5669667720360252 1105 32-33 0.5627594743268602 1105 34-35 0.6185258377586749 1105 36-37 0.6789150758990274 1105 38-39 0.6796678482037848 1105 40-41 0.6628679674828746 1105 42-43 0.8134045619278751 1105 44-45 0.8440185871814165 1105 46-47 0.7464301789706127 1105 48-49 0.8395736721961455 1105 50-51 0.9041427193868472 1105 52-53 0.9453904307768042 1105 54-55 1.0534281099332503 1105 56-57 1.093607566528263 1105 58-59 1.2281325182575102 1105 60-61 1.1799172834128129 1105 62-63 1.2724771793439942 1105 64-65 1.3482857973912488 1105 66-67 1.3753007651685607 1105 68-69 1.5929036923219613 1105 70-71 1.4985066380757672 1105 72-73 1.6138928556888281 1105 74-75 1.7840448564780829 1105 76-77 1.6599309862899752 1105 78-79 1.920131367832763 1105 80-81 2.2340156383729486 1105 82-83 2.186230590247124 1105 84-85 2.3398978443991716 1105 86-87 2.6435343431175404 1105 88-89 2.686263100014486 1105 90-91 2.7838992831198794 1105 92-93 2.9978018940307507 1105 94-95 2.9438088916887217 1105 96-97 3.55511437637778 1105 98-99 3.8689083333856047 1105 100-101 4.17161682176895 1106 1 0.15116094234263855 1106 2 0.1304406481712519 1106 3 0.12742004099983006 1106 4 0.11643912697294212 1106 5 0.06615339036408585 1106 6 0.08984065173797973 1106 7 0.12271734901075604 1106 8 0.08359119900512724 1106 9 0.16927549877168246 1106 10-11 0.16607898241154828 1106 12-13 0.1307168467669939 1106 14-15 0.1117595011317647 1106 16-17 0.08851134530024751 1106 18-19 0.2507845870707044 1106 20-21 0.40486784359757877 1106 22-23 0.24575830514002206 1106 24-25 0.15055856172299542 1106 26-27 0.2181805491246891 1106 28-29 0.29900786580837035 1106 30-31 0.36355692554317187 1106 32-33 0.44601663107889067 1106 34-35 0.3967229072842855 1106 36-37 0.5402009443761671 1106 38-39 0.4551349706935284 1106 40-41 0.5024585647237529 1106 42-43 0.5940126305550351 1106 44-45 0.10571731349615732 1106 46-47 -0.19756268969244672 1106 48-49 0.28453798116670725 1106 50-51 0.19502260583863062 1106 52-53 0.47832037396606353 1106 54-55 0.5498744048710265 1106 56-57 0.4960044041583487 1106 58-59 0.8835421889054231 1106 60-61 0.7461559920148346 1106 62-63 0.6997261400728689 1106 64-65 0.39005068994723757 1106 66-67 0.5882911275831688 1106 68-69 0.564553216395776 1106 70-71 0.7305896863479475 1106 72-73 1.227433168601248 1106 74-75 1.417885415481848 1106 76-77 1.4330916893400598 1106 78-79 1.58841778857294 1106 80-81 1.8055956945334266 1106 82-83 1.9604477367781747 1106 84-85 1.9168786072365407 1106 86-87 2.0852371060812978 1106 88-89 2.4511764345567535 1106 90-91 2.480166974083371 1106 92-93 2.0584627917008937 1106 94-95 2.6932957816235223 1106 96-97 3.061394880210191 1106 98-99 3.1504224728764427 1106 100-101 3.687301424162456 1107 1 0.2065145824531811 1107 2 0.15445726204259813 1107 3 0.16580072191890594 1107 4 0.15241198280907753 1107 5 0.04685726008059987 1107 6 0.03769268674246007 1107 7 0.17166993016073206 1107 8 0.1411530357023807 1107 9 0.22955600115383845 1107 10-11 0.26626316293767616 1107 12-13 0.2844871142049996 1107 14-15 0.38238685691954544 1107 16-17 0.4124004470229181 1107 18-19 0.3358606306994929 1107 20-21 0.33689505991098656 1107 22-23 0.288537618493244 1107 24-25 0.30513442530989465 1107 26-27 0.36186708767943543 1107 28-29 0.30127194509496746 1107 30-31 0.20616346729374158 1107 32-33 0.3490074178475524 1107 34-35 0.49486521141764683 1107 36-37 0.5001368109552757 1107 38-39 0.5041573222242661 1107 40-41 0.5500910415800178 1107 42-43 0.5992388802415931 1107 44-45 0.5509549787457289 1107 46-47 0.7916617425337904 1107 48-49 0.6981777420285837 1107 50-51 0.6175530276058367 1107 52-53 0.8569045852110762 1107 54-55 0.9487376732941115 1107 56-57 0.8938512160669205 1107 58-59 0.9509520512170866 1107 60-61 0.9709423817777392 1107 62-63 0.9894257633681462 1107 64-65 1.1495719954202173 1107 66-67 1.336374074329946 1107 68-69 1.6203064454752294 1107 70-71 1.4304152105995982 1107 72-73 1.5532000950284122 1107 74-75 1.4760408260448514 1107 76-77 1.32640198793516 1107 78-79 1.7337421328696827 1107 80-81 1.922791454176295 1107 82-83 2.007456651230722 1107 84-85 2.2079247822815162 1107 86-87 2.527683679453734 1107 88-89 2.5521117670964415 1107 90-91 2.5662279364653493 1107 92-93 2.8469248621759498 1107 94-95 3.0190560685219836 1107 96-97 3.124885594718645 1107 98-99 3.4706734448481136 1107 100-101 3.845131431423887 1108 1 0.17048683065435455 1108 2 0.14687008260369794 1108 3 0.18600990654243788 1108 4 0.13335605448169474 1108 5 0.06644901739463194 1108 6 -0.08864746109696142 1108 7 0.04677553722087424 1108 8 0.16558127206970852 1108 9 0.09035344691710634 1108 10-11 0.12267082815858998 1108 12-13 0.13950467876706085 1108 14-15 0.348466434114691 1108 16-17 0.29160500983375215 1108 18-19 0.3154427426733122 1108 20-21 0.3147528319420161 1108 22-23 0.2290383183557836 1108 24-25 0.2938283500009149 1108 26-27 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0.3520396576042657 2316 66-67 0.5217958493054482 2316 68-69 0.6698913081283528 2316 70-71 0.7683552231895199 2316 72-73 0.8730785870810216 2316 74-75 1.0536058951117298 2316 76-77 0.9516291648996713 2316 78-79 1.067426067523943 2316 80-81 1.0887810319571152 2316 82-83 1.400692996499096 2316 84-85 1.4659219825475311 2316 86-87 1.4749887768479262 2316 88-89 1.624109731387776 2316 90-91 1.78834640607948 2316 92-93 1.7903390781235196 2316 94-95 2.476333155884852 2316 96-97 2.4416083575966603 2316 98-99 2.7743646263206294 2316 100-101 3.324193710909938 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 16.0 4 44.0 5 102.0 6 181.0 7 450.0 8 1129.0 9 2153.0 10 3362.0 11 5351.0 12 7530.0 13 10510.0 14 13770.0 15 17872.0 16 22342.0 17 27190.0 18 27057.0 19 25218.0 20 25455.0 21 27827.0 22 30580.0 23 34135.0 24 39617.0 25 46325.0 26 54943.0 27 65721.0 28 78654.0 29 93138.0 30 110985.0 31 131068.0 32 157291.0 33 192570.0 34 240790.0 35 307729.0 36 395645.0 37 449735.0 38 255447.0 39 16321.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.2037972020256 18.920628567629823 14.934649280014694 23.94092495032989 2 15.998565636855181 20.026164100194244 38.97062503813884 25.00464522481174 3 19.518841276972097 23.091362725636316 29.685218557579258 27.70457743981233 4 13.617996502020386 16.655118673618762 35.945681256202334 33.78120356815852 5 14.214609264185537 36.89212250939471 34.170632940050524 14.722635286369226 6 33.534480868025284 35.74299170463455 16.593540799710514 14.128986627629653 7 29.602440635776983 31.891650355554823 19.94948352714772 18.55642548152047 8 26.875435191429403 35.07475012473203 19.205237648787495 18.84457703505107 9 26.256640952678584 16.603752378132693 19.567063341941214 37.5725433272475 10-11 25.332595906596268 26.148425635036816 27.15926909770767 21.35970936065924 12-13 26.17768968863595 23.229404822606377 27.31398479091622 23.27892069784145 14-15 22.600142002620743 24.98315774901174 25.329666074532188 27.087034173835335 16-17 22.74096241042595 28.053604618648947 26.302712990224297 22.9027199807008 18-19 22.86778473170277 26.60619904490901 28.18519005872 22.34082616466821 20-21 23.07679655246147 25.94890578482491 28.464946187037736 22.509351475675885 22-23 22.845202751232243 25.93417095690029 28.37842190678268 22.84220438508479 24-25 22.894333253433206 26.141973558255334 28.333852769824414 22.629840418487053 26-27 22.43653013105226 26.450982206503266 28.082538246422107 23.029949416022372 28-29 22.586692188759315 26.827324264903424 27.954881271553973 22.631102274783295 30-31 22.717335285184028 26.326391516028753 27.898289252210912 23.05798394657631 32-33 22.30126695837127 26.31977257595222 27.94350310751413 23.435457358162378 34-35 23.177350238047055 26.509529164983753 27.676090815511863 22.637029781457333 36-37 23.314843971270296 25.95350604806549 27.74214750402179 22.989502476642425 38-39 22.68953613283007 26.277638303111473 27.78701484478443 23.245810719274022 40-41 23.211094376219776 26.089552801437943 27.891917732873438 22.807435089468843 42-43 22.93103956399146 26.173037461259145 27.63771937159283 23.258203603156566 44-45 22.81696275283721 26.387507701712913 27.884655583510554 22.91087396193932 46-47 23.352855841443628 26.63130421582346 27.161278373329544 22.854561569403366 48-49 23.000106913170058 26.740217444939717 27.088319187898524 23.1713564539917 50-51 23.13943670466196 26.800527438305615 27.18558618572188 22.874449671310536 52-53 23.48250119249305 26.458593762850143 27.21954482403189 22.839360220624922 54-55 22.89436666775179 26.72425816112711 27.36729655222042 23.01407861890068 56-57 22.99501825747981 26.76136020965947 27.20503273187822 23.038588800982502 58-59 22.916323996249815 26.428010428146127 27.145904951450454 23.509760624153603 60-61 22.509557077925994 26.683728226721716 27.546298200020836 23.26041649533146 62-63 22.7946931317883 26.72412906886853 27.551078452335915 22.930099347007253 64-65 22.41700179833435 26.802720528973467 27.299901036783613 23.48037663590857 66-67 22.361757902852737 26.78939432879294 27.293155144350212 23.555692624004113 68-69 23.036614409320656 26.356703503812216 27.37750364088066 23.229178445986463 70-71 22.679264601871804 26.470947031377644 27.060699952300276 23.789088414450273 72-73 22.699109108164105 26.60375350958818 27.078163649481684 23.61897373276603 74-75 22.68190316408156 26.87068920615601 26.87953010291078 23.567877526851653 76-77 22.70093850573767 26.489571168533537 27.209247578005492 23.6002427477233 78-79 22.786845979242397 26.6160508193935 27.15628786508106 23.44081533628304 80-81 22.908515035780795 26.63848316228651 26.91030651702704 23.54269528490566 82-83 22.829481670333283 26.729170521756412 27.009975258890695 23.431372549019606 84-85 22.660969164746767 26.7746779520109 26.970351812005056 23.594001071237276 86-87 22.8684433130064 26.831792807483072 26.699314494981262 23.600449384529266 88-89 22.646713179646664 26.88817129834677 26.49879457010846 23.966320951898112 90-91 22.50879087007126 26.851925304632797 26.75100784671786 23.888275978578086 92-93 22.67040018531667 26.50850784443269 26.93593896063666 23.885153009613987 94-95 22.319854735156888 26.25398525694799 27.164443421002133 24.26171658689299 96-97 22.554721038867047 25.92387371087389 27.15596232818505 24.365442922074006 98-99 22.261823500063052 26.228387776809164 26.81330904485419 24.6964796782736 100-101 22.42454148344423 25.913627808518314 27.111883265355775 24.54994744268168 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 178.0 1 138.5 2 128.0 3 173.5 4 173.5 5 173.0 6 292.5 7 659.0 8 1031.5 9 1172.0 10 1107.0 11 1002.0 12 1038.5 13 1157.5 14 1326.0 15 1501.0 16 1688.5 17 1916.5 18 2137.5 19 2513.0 20 3027.0 21 3610.0 22 4363.0 23 5489.5 24 6808.0 25 8829.5 26 12054.5 27 15904.5 28 19567.5 29 23586.5 30 28343.0 31 34935.5 32 45195.5 33 49753.5 34 49670.5 35 53667.5 36 60474.5 37 67362.5 38 72854.0 39 77287.5 40 82562.0 41 88810.0 42 92289.5 43 95555.5 44 99955.5 45 106416.5 46 109740.5 47 111702.5 48 113385.5 49 111581.5 50 110533.5 51 109400.5 52 108122.5 53 108884.0 54 110227.5 55 104330.5 56 94949.0 57 86969.0 58 80266.5 59 70550.5 60 56959.5 61 47195.5 62 40182.0 63 30816.5 64 21545.0 65 15726.5 66 11688.5 67 8953.5 68 6894.0 69 5291.5 70 4065.5 71 2817.5 72 1992.0 73 1634.5 74 1242.0 75 906.5 76 680.0 77 468.0 78 322.0 79 202.0 80 107.0 81 60.0 82 37.5 83 16.5 84 7.5 85 3.0 86 2.0 87 2.0 88 1.0 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.853408337034174E-5 2 0.04393034744038905 3 3.084033751665378E-4 4 0.0 5 0.10454874418145631 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0010108777297125407 26-27 0.011513726006217412 28-29 0.0 30-31 0.0 32-33 2.398692917961961E-4 34-35 0.015437302279169477 36-37 0.0031354343141931345 38-39 0.012421802610874439 40-41 0.006887675378719345 42-43 0.005071522169405289 44-45 0.0025357610847026443 46-47 1.7133520842585434E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 3.426704168517087E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.056022501110252E-4 68-69 2.056022501110252E-4 70-71 0.0 72-73 1.7133520842585434E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.7133520842585434E-5 82-83 0.0012336135006661513 84-85 0.004626050627498067 86-87 0.0014563492716197619 88-89 8.566760421292717E-5 90-91 0.0010108777297125407 92-93 2.7413633348136694E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 4.6260506274980675E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2918256.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.80066156365621 #Duplication Level Percentage of deduplicated Percentage of total 1 73.45008893949283 38.04763198975116 2 14.493071880423688 15.0150142299114 3 5.094366536283524 7.916746704817151 4 2.3898765341155275 4.951887420905685 5 1.2678362580607625 3.283737846096893 6 0.7560120824602301 2.3497155612934395 7 0.5000447189818389 1.8131853078270272 8 0.35460380754941473 1.469496945924089 9 0.26626132919816564 1.241326170115509 >10 1.2907164047285558 12.024109132834536 >50 0.08751163416286394 3.1158582508271517 >100 0.043645341827446504 4.235281062238601 >500 0.002518358257684724 0.8835075595028832 >1k 0.0033799018718573166 3.462899228165802 >5k 6.627258572269248E-5 0.1896025897886403 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5523 0.18925687122719873 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4696 0.1609180277535624 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4038 0.13837031432471997 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3994 0.13686256449057246 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3983 0.13648562703203557 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3791 0.12990635502848277 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3695 0.12661671902670638 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3290 0.11273856714421215 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3043 0.10427460784797496 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.426704168517087E-5 2 0.0 0.0 0.0 0.0 3.426704168517087E-5 3 0.0 0.0 0.0 0.0 3.426704168517087E-5 4 0.0 0.0 0.0 0.0 3.426704168517087E-5 5 0.0 0.0 0.0 0.0 3.426704168517087E-5 6 0.0 0.0 0.0 0.0 3.426704168517087E-5 7 0.0 0.0 0.0 0.0 6.853408337034174E-5 8 0.0 0.0 0.0 3.426704168517087E-5 6.853408337034174E-5 9 0.0 0.0 0.0 6.853408337034174E-5 6.853408337034174E-5 10-11 0.0 0.0 0.0 6.853408337034174E-5 6.853408337034174E-5 12-13 0.0 0.0 0.0 6.853408337034174E-5 3.084033751665378E-4 14-15 0.0 0.0 0.0 6.853408337034174E-5 3.94070979379465E-4 16-17 0.0 0.0 0.0 1.0280112505551261E-4 4.454715419072213E-4 18-19 0.0 0.0 0.0 1.1993464589809803E-4 4.797385835923922E-4 20-21 0.0 0.0 0.0 1.8846872926843978E-4 5.996732294904902E-4 22-23 0.0 0.0 0.0 3.7693745853687957E-4 7.024743545460028E-4 24-25 0.0 0.0 0.0 6.339402711756611E-4 7.196078753885883E-4 26-27 0.0 0.0 0.0 0.0011308123756106386 7.196078753885883E-4 28-29 0.0 0.0 0.0 0.0031697013558783056 7.196078753885883E-4 30-31 0.0 0.0 0.0 0.011428058402004485 7.367413962311737E-4 32-33 0.0 0.0 0.0 0.0300007949953671 8.224090004441009E-4 34-35 0.0 0.0 0.0 0.059299115636188185 8.224090004441009E-4 36-37 0.0 0.0 0.0 0.1092775959340099 8.395425212866863E-4 38-39 0.0 0.0 0.0 0.18509342566245046 8.738095629718572E-4 40-41 0.0 0.0 0.0 0.269133345395332 8.909430838144426E-4 42-43 0.0 0.0 0.0 0.35384147244107444 9.252101254996135E-4 44-45 0.0 0.0 0.0 0.46037770504027065 9.937442088699551E-4 46-47 0.0 0.0 0.0 0.5892046482556705 0.0010794118130828824 48-49 0.0 0.0 0.0 0.71995054580544 0.0011308123756106386 50-51 0.0 0.0 0.0 0.8700230548656458 0.0011650794172958095 52-53 0.0 0.0 0.0 1.008958775378171 0.0012336135006661513 54-55 0.0 0.0 0.0 1.163485999857449 0.0013192811048790784 56-57 0.0 0.0 0.0 1.3469688745606965 0.0013706816674068348 58-59 0.0 0.0 0.0 1.546522991814289 0.0013706816674068348 60-61 0.0 0.0 0.0 1.7603321984089129 0.0013706816674068348 62-63 0.0 0.0 0.0 1.9713829081478802 0.0014049487090920056 64-65 0.0 0.0 0.0 2.188738753556919 0.0015248833549901036 66-67 0.0 0.0 0.0 2.4464440405502463 0.001610550959203031 68-69 0.0 0.0 0.0 2.734270057184839 0.0016448180008882018 70-71 0.0 0.0 0.0 3.0388526572034804 0.0016790850425733726 72-73 0.0 0.0 0.0 3.3536468356443025 0.001884687292684398 74-75 0.0 0.0 0.0 3.66695039777182 0.002210224188693521 76-77 0.0 1.7133520842585434E-5 0.0 4.0122422433124445 0.0022616247512212773 78-79 0.0 3.426704168517087E-5 0.0 4.401464436293457 0.0023130253137490335 80-81 0.0 3.426704168517087E-5 0.0 4.806380934366279 0.0023644258762767897 82-83 0.0 3.426704168517087E-5 0.0 5.221252008048642 0.0025014940430174736 84-85 0.0 3.426704168517087E-5 0.0 5.655055622262063 0.0025014940430174736 86-87 0.0 3.426704168517087E-5 0.0 6.105701487463746 0.002604295168072986 88-89 0.0 3.426704168517087E-5 0.0 6.58180776463751 0.002758496855656255 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4205 0.0 50.223816 1 GTATCAA 7145 0.0 38.478516 1 ATCAACG 9190 0.0 29.747559 3 TCAACGC 9325 0.0 29.316898 4 TATCAAC 9420 0.0 29.034187 2 CAACGCA 9450 0.0 28.979422 5 AACGCAG 9860 0.0 27.793535 6 TAAGGTG 1945 0.0 26.888842 5 GTAAGGT 1925 0.0 24.94535 4 ACGCAGA 11295 0.0 24.136293 7 CGCAGAG 11475 0.0 23.633514 8 GTACATG 9075 0.0 22.6952 1 GTATAGG 655 0.0 22.511951 1 TACATGG 8965 0.0 22.390043 2 GGTAAGG 2005 0.0 22.290125 3 AGGTAAG 1945 0.0 21.765224 2 ACATGGG 8885 0.0 21.40433 3 GCAGAGT 13085 0.0 20.58042 9 TGGTATC 2135 0.0 20.49664 2 AGAGTAC 11020 0.0 20.1917 10-11 >>END_MODULE