FastQCFastQC Report
Thu 26 May 2016
SRR938468_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938468_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2928363
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55640.19000376660953577No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44860.1531913905482346No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA41040.1401465596990537No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA40180.13720976531939516No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA36340.12409663692650125No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA35100.11986218921629593No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC32170.10985659906234302No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG30040.10258291065690969No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA29470.10063643066108949No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41600.047.4804571
GTATCAA72800.040.4353941
ATCAACG92850.031.1238563
TATCAAC95500.030.3597472
TCAACGC95950.030.1678284
CAACGCA97950.029.4542885
AACGCAG101800.028.520286
CGCAGAG115200.025.1138848
ACGCAGA115600.025.0269857
TAAGGTG18050.025.0155855
GTAAGGT17350.024.3816014
GTACATG91600.023.6465781
TACATGG89800.023.1300622
AGGTAAG17950.022.5074442
GGTAAGG19100.021.8988363
GCAGAGT131750.021.4893079
ACATGGG93550.021.2375373
GAGTACT73950.020.7155312-13
AGAGTAC113400.020.44108210-11
GGACCGA7450.019.7726926
Produced by FastQC (version 0.11.5)