##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938468_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2928363 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.940594113503003 31.0 31.0 34.0 28.0 34.0 2 31.130304883650012 31.0 31.0 34.0 27.0 34.0 3 31.187477440467593 31.0 31.0 34.0 27.0 34.0 4 34.85278532750209 37.0 35.0 37.0 32.0 37.0 5 34.83619482967105 37.0 35.0 37.0 32.0 37.0 6 34.80162432048213 37.0 35.0 37.0 32.0 37.0 7 34.713321060264725 37.0 35.0 37.0 31.0 37.0 8 34.65251712304793 37.0 35.0 37.0 30.0 37.0 9 36.119331175813926 39.0 35.0 39.0 31.0 39.0 10-11 35.944612399487355 38.0 35.0 39.0 30.0 39.0 12-13 35.76790872579663 38.0 35.0 39.0 30.0 39.0 14-15 36.917497420914 39.0 36.0 41.0 31.0 41.0 16-17 36.84121043053747 39.0 36.0 41.0 30.5 41.0 18-19 36.81394502662409 39.0 36.0 41.0 30.0 41.0 20-21 36.726290763815825 39.0 36.0 41.0 30.0 41.0 22-23 36.572988901990634 39.0 36.0 41.0 30.0 41.0 24-25 36.43640030283131 39.0 36.0 41.0 29.5 41.0 26-27 36.21840666611345 39.0 35.0 40.5 29.0 41.0 28-29 35.97826669712737 39.0 35.0 40.0 27.0 41.0 30-31 35.70005477463006 38.0 34.5 40.0 27.0 41.0 32-33 35.39528552300381 38.0 34.0 40.0 26.5 41.0 34-35 35.10052886202974 38.0 34.0 40.0 25.5 41.0 36-37 34.88360510633415 38.0 34.0 40.0 25.0 41.0 38-39 34.63530187343578 38.0 33.5 40.0 24.0 41.0 40-41 34.353613093731894 38.0 33.0 40.0 24.0 41.0 42-43 33.935639980425925 38.0 33.0 40.0 22.5 41.0 44-45 33.48664748871639 38.0 32.0 40.0 20.0 41.0 46-47 33.023015589255834 37.0 31.5 40.0 17.0 41.0 48-49 32.54875181116549 37.0 31.0 40.0 14.5 41.0 50-51 31.823573955824465 36.0 30.0 38.5 12.0 40.5 52-53 31.823393308821345 36.0 30.0 39.0 9.0 40.0 54-55 31.79810238689671 36.0 30.0 39.0 8.0 40.0 56-57 31.368051877448252 36.0 29.5 39.0 4.5 40.0 58-59 30.91762018574883 35.5 29.0 39.0 2.0 40.0 60-61 30.36067335231322 35.0 28.0 39.0 2.0 40.0 62-63 29.62872020989201 34.5 27.5 38.0 2.0 40.0 64-65 28.960845018189342 34.0 26.0 38.0 2.0 40.0 66-67 28.257491984429528 34.0 25.5 37.0 2.0 39.5 68-69 27.423282222866497 33.0 23.5 36.5 2.0 39.0 70-71 26.592479313527726 32.5 21.5 36.0 2.0 39.0 72-73 25.69443627719651 31.5 19.0 35.5 2.0 38.5 74-75 24.770627651011846 31.0 11.0 35.0 2.0 37.5 76-77 23.83158952629848 30.5 6.5 34.5 2.0 36.5 78-79 22.86045992248912 29.5 2.0 34.0 2.0 36.0 80-81 21.924017616668426 29.0 2.0 34.0 2.0 35.5 82-83 20.936635075637824 27.5 2.0 34.0 2.0 35.0 84-85 19.984637492004918 26.0 2.0 33.0 2.0 35.0 86-87 19.019618127943836 25.5 2.0 32.5 2.0 35.0 88-89 17.94520488067907 23.0 2.0 32.0 2.0 34.0 90-91 16.81610322900542 19.0 2.0 31.5 2.0 34.0 92-93 15.68369426877747 8.0 2.0 31.0 2.0 34.0 94-95 14.446572197504203 2.0 2.0 30.5 2.0 34.0 96-97 13.046652003184033 2.0 2.0 29.5 2.0 34.0 98-99 11.360124410805629 2.0 2.0 28.0 2.0 34.0 100-101 8.062685534546093 2.0 2.0 10.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6429903202896874 1101 2 0.4727632980420964 1101 3 0.5307601086177804 1101 4 0.3320858420737878 1101 5 0.38401862897850947 1101 6 0.4595685141529273 1101 7 0.39594888814603024 1101 8 0.4106564327318978 1101 9 0.5396116987993054 1101 10-11 0.6398155484474017 1101 12-13 0.5491602185608357 1101 14-15 0.6446284129234598 1101 16-17 0.7274123136512998 1101 18-19 0.6390445322845011 1101 20-21 0.7995408565340867 1101 22-23 0.8331935244312021 1101 24-25 0.8441874956220587 1101 26-27 0.9373461916851582 1101 28-29 0.9076971265479798 1101 30-31 1.052896159537596 1101 32-33 1.1216944558450734 1101 34-35 1.221749828083297 1101 36-37 1.302411971625176 1101 38-39 1.316788698504368 1101 40-41 1.2563715085210987 1101 42-43 1.495575157744888 1101 44-45 1.5542518287779288 1101 46-47 1.5540903895447826 1101 48-49 1.4368376236960856 1101 50-51 1.419915552312009 1101 52-53 1.4453279390419702 1101 54-55 1.4720151241279673 1101 56-57 1.5994563270231055 1101 58-59 1.6371225076516538 1101 60-61 1.6893476533785439 1101 62-63 1.587778712352364 1101 64-65 1.650840020996121 1101 66-67 1.875162138533458 1101 68-69 2.1120160834350017 1101 70-71 2.5689969631533778 1101 72-73 2.5475202844756417 1101 74-75 2.638341027557278 1101 76-77 2.7194193766177364 1101 78-79 2.9191750237702756 1101 80-81 3.0940312528718756 1101 82-83 3.291121481626103 1101 84-85 3.3310551422772328 1101 86-87 3.485513804662723 1101 88-89 3.552569863695659 1101 90-91 3.6063864855798364 1101 92-93 3.522880005343911 1101 94-95 3.5162586749356546 1101 96-97 3.2600490044269925 1101 98-99 2.854354854588351 1101 100-101 2.2505926228792976 1102 1 0.703512968504814 1102 2 0.6039492988440252 1102 3 0.6398709694825158 1102 4 0.5476006343371225 1102 5 0.5493876830224877 1102 6 0.6321203014226242 1102 7 0.6252219097026881 1102 8 0.7012650923294004 1102 9 0.8297895922173382 1102 10-11 0.8754100962229572 1102 12-13 0.8033461661351566 1102 14-15 0.8946609155981875 1102 16-17 1.008279410832536 1102 18-19 0.9409149294470112 1102 20-21 0.9585477153467608 1102 22-23 0.8319965540578949 1102 24-25 0.9426133380530004 1102 26-27 0.9401148079093176 1102 28-29 0.9472623494278238 1102 30-31 0.7443842849017557 1102 32-33 0.8506766126249659 1102 34-35 0.9654813017213755 1102 36-37 1.0222466589221213 1102 38-39 1.2145948883410753 1102 40-41 1.2508960929716437 1102 42-43 1.3939368537559247 1102 44-45 1.4043846960682416 1102 46-47 1.5544050700952212 1102 48-49 1.5571243942420452 1102 50-51 1.6272882781496314 1102 52-53 1.6407823667782075 1102 54-55 1.6846120286239845 1102 56-57 1.8890772176471486 1102 58-59 2.042983309269772 1102 60-61 2.0515760055235184 1102 62-63 2.1540016594879816 1102 64-65 2.1641272705905017 1102 66-67 2.274124824942316 1102 68-69 2.2993676822087075 1102 70-71 2.2367904759708885 1102 72-73 2.22696146751834 1102 74-75 2.2633992818506563 1102 76-77 2.457428362322158 1102 78-79 2.818760434097186 1102 80-81 2.974694054962928 1102 82-83 3.2039103959170383 1102 84-85 3.237267128951327 1102 86-87 3.190733935940994 1102 88-89 3.214505542106192 1102 90-91 3.0761776733010997 1102 92-93 2.9661332634523667 1102 94-95 2.9582309567587632 1102 96-97 3.0043309087466668 1102 98-99 2.862495814816919 1102 100-101 2.4667374269477094 1103 1 0.5154523913413804 1103 2 0.4824462969443566 1103 3 0.42698543397650823 1103 4 0.2981570306693584 1103 5 0.4063571097670291 1103 6 0.5045741437888154 1103 7 0.4730211704562919 1103 8 0.4846117791747062 1103 9 0.565821592362191 1103 10-11 0.5596128035406238 1103 12-13 0.49262088182606334 1103 14-15 0.644714291924295 1103 16-17 0.576420798119095 1103 18-19 0.7121619135902861 1103 20-21 0.6779969043535203 1103 22-23 0.7928409637647675 1103 24-25 0.7887798113326525 1103 26-27 0.9167229630575164 1103 28-29 0.8529190957679376 1103 30-31 0.794034138717258 1103 32-33 0.9569192663072812 1103 34-35 0.8658634108318992 1103 36-37 0.9973830357496709 1103 38-39 0.9348686755730995 1103 40-41 1.0900913917306951 1103 42-43 1.0779021024355373 1103 44-45 1.1049995234458052 1103 46-47 1.417173215405576 1103 48-49 1.2414673008615082 1103 50-51 1.3070883501730997 1103 52-53 1.3588613119362627 1103 54-55 1.5500360390935377 1103 56-57 1.7078100196565629 1103 58-59 1.754860352847487 1103 60-61 1.7510708730974152 1103 62-63 1.9472129075207931 1103 64-65 1.8860244148501764 1103 66-67 2.1099742642205577 1103 68-69 2.2296102936426365 1103 70-71 2.2105183842073615 1103 72-73 2.4401752454255465 1103 74-75 2.6135492385209 1103 76-77 2.5464582510529503 1103 78-79 2.6392557865217654 1103 80-81 2.48970091278348 1103 82-83 2.621730783385157 1103 84-85 2.658165739834015 1103 86-87 2.7043625025986593 1103 88-89 2.8696236842227663 1103 90-91 2.9017845791037686 1103 92-93 3.0269254410606123 1103 94-95 3.046706083768017 1103 96-97 2.9014700131107745 1103 98-99 2.4923889590584896 1103 100-101 1.697392752433906 1104 1 0.506106234382873 1104 2 0.5092523860242828 1104 3 0.44470931190930685 1104 4 0.375768523379314 1104 5 0.453194996106113 1104 6 0.5507444304877609 1104 7 0.45193525597568396 1104 8 0.46789060972101737 1104 9 0.5698957351928158 1104 10-11 0.6232260051152565 1104 12-13 0.625416803399176 1104 14-15 0.6720312553812491 1104 16-17 0.6499838523695942 1104 18-19 0.7101658977678156 1104 20-21 0.658625685633119 1104 22-23 0.6341743066182346 1104 24-25 0.7223688793587897 1104 26-27 0.7796162042359143 1104 28-29 0.9368923013410253 1104 30-31 0.8081785639627554 1104 32-33 0.8412910086352383 1104 34-35 0.7847895631255355 1104 36-37 0.7994516426203546 1104 38-39 0.9571528183561284 1104 40-41 0.9662436853698182 1104 42-43 1.0993226497482382 1104 44-45 0.9580115759398424 1104 46-47 1.0415895119769019 1104 48-49 1.0637623918026193 1104 50-51 0.9980019739658239 1104 52-53 1.14050291684104 1104 54-55 1.45025119420038 1104 56-57 1.7219611089982934 1104 58-59 1.7580044972489333 1104 60-61 1.7820978341267413 1104 62-63 1.6847345063326777 1104 64-65 1.640164484632784 1104 66-67 1.9673022710369743 1104 68-69 2.10657774905269 1104 70-71 2.3848390033971008 1104 72-73 2.5753144298353696 1104 74-75 2.5638631650539985 1104 76-77 2.6147824926549994 1104 78-79 2.8247218686519915 1104 80-81 2.940514884537233 1104 82-83 3.078411896860434 1104 84-85 3.1296401949251447 1104 86-87 3.1960927161829424 1104 88-89 3.340381153960628 1104 90-91 3.060776110941486 1104 92-93 3.08294216794215 1104 94-95 3.173626057492436 1104 96-97 3.168513439217847 1104 98-99 2.8138098633763473 1104 100-101 2.152598958611522 1105 1 0.43214406259222216 1105 2 0.3843977658247617 1105 3 0.336541074732871 1105 4 0.31200401372736053 1105 5 0.3040022335952912 1105 6 0.4240691067542528 1105 7 0.38525091257567823 1105 8 0.48066522230594444 1105 9 0.5017390105555251 1105 10-11 0.5089535160045742 1105 12-13 0.5193111917162128 1105 14-15 0.6319244471194096 1105 16-17 0.6708908954147077 1105 18-19 0.588724700101146 1105 20-21 0.5845957530313299 1105 22-23 0.544942043407147 1105 24-25 0.668357733951801 1105 26-27 0.6183300632481021 1105 28-29 0.7164058738994825 1105 30-31 0.7110425347009652 1105 32-33 0.9412782043348145 1105 34-35 1.0115137245005101 1105 36-37 0.8636640761626353 1105 38-39 1.0166711726975777 1105 40-41 1.0687583139997798 1105 42-43 1.1678581777160062 1105 44-45 1.1746609058103488 1105 46-47 1.4110457031394361 1105 48-49 1.2999363535211614 1105 50-51 1.2001680536743002 1105 52-53 1.2514909329223336 1105 54-55 1.6307174973608873 1105 56-57 1.7297043113878274 1105 58-59 1.761862330987924 1105 60-61 1.8090641440859905 1105 62-63 1.7750848229275036 1105 64-65 1.8199595251759604 1105 66-67 2.1914753171913794 1105 68-69 2.4045562559579494 1105 70-71 2.530107531106516 1105 72-73 2.6431853806169947 1105 74-75 2.604485771329692 1105 76-77 2.714893992049909 1105 78-79 2.6279453796138306 1105 80-81 2.7960209603477573 1105 82-83 3.140381424891654 1105 84-85 3.163662580921546 1105 86-87 3.225915065012881 1105 88-89 3.2729351246535145 1105 90-91 3.1227646754547678 1105 92-93 3.403283328895604 1105 94-95 3.355980145123711 1105 96-97 3.004718108594947 1105 98-99 2.693091032216346 1105 100-101 2.0140487226951187 1106 1 0.4259931343901364 1106 2 0.35616936736187554 1106 3 0.14574795289398423 1106 4 0.3208719109328655 1106 5 0.2988636566972076 1106 6 0.02808698912547669 1106 7 0.41297997858116275 1106 8 0.017754454031525313 1106 9 0.3393460292891035 1106 10-11 0.25492066690452475 1106 12-13 0.37525046653183836 1106 14-15 0.4947133182307155 1106 16-17 0.033452395866795825 1106 18-19 0.5997371661942239 1106 20-21 0.5675705471174055 1106 22-23 0.47379356992519206 1106 24-25 0.53036802611647 1106 26-27 0.46735572478635135 1106 28-29 0.5561020537192078 1106 30-31 0.4143801152299176 1106 32-33 0.5916582886076469 1106 34-35 0.44948722941701647 1106 36-37 0.520759100321321 1106 38-39 0.5444525560294906 1106 40-41 0.7587902960008535 1106 42-43 0.32622758130731455 1106 44-45 0.6850148799845712 1106 46-47 0.9388657174085964 1106 48-49 0.6713931239571522 1106 50-51 0.5959565751204217 1106 52-53 0.8134039634244452 1106 54-55 0.6686404950862972 1106 56-57 1.2056961772576784 1106 58-59 1.172465706493 1106 60-61 1.5507730619605908 1106 62-63 1.5036342775911216 1106 64-65 1.3701541368003838 1106 66-67 1.700823121011556 1106 68-69 1.6477803895653622 1106 70-71 1.4888048782427923 1106 72-73 1.840725892329015 1106 74-75 1.8928140627738408 1106 76-77 2.070939226007699 1106 78-79 2.3805166547967893 1106 80-81 2.249695181901263 1106 82-83 2.3513421034540016 1106 84-85 2.3302113680425656 1106 86-87 2.1811380560275424 1106 88-89 2.283834788409834 1106 90-91 2.3757911334525588 1106 92-93 2.4844798195288185 1106 94-95 2.331272901403553 1106 96-97 2.269409343654708 1106 98-99 2.3158321157545334 1106 100-101 1.4853967084118267 1107 1 0.22972620057946713 1107 2 0.26973785860850086 1107 3 0.19155378361690012 1107 4 0.19465187987906774 1107 5 0.1905192629920407 1107 6 0.12805177391860667 1107 7 0.22117045937255853 1107 8 0.2433233215850592 1107 9 0.2109677425364609 1107 10-11 0.2385967658223862 1107 12-13 0.20805938568349092 1107 14-15 0.2899961120213064 1107 16-17 0.35709953440876774 1107 18-19 0.4380863357764184 1107 20-21 0.43096997570723516 1107 22-23 0.45785159068647374 1107 24-25 0.38443510705974404 1107 26-27 0.46357242077782956 1107 28-29 0.37992007712481524 1107 30-31 0.46848599222840903 1107 32-33 0.6441737604151427 1107 34-35 0.6447624377418748 1107 36-37 0.5839261127835229 1107 38-39 0.6524768981704412 1107 40-41 0.5764350639788276 1107 42-43 0.718585478836566 1107 44-45 0.6568757684756861 1107 46-47 0.7160163876417087 1107 48-49 0.6538968658377371 1107 50-51 0.7946537184086075 1107 52-53 0.8847339328791683 1107 54-55 0.943486815245496 1107 56-57 1.1733208585833665 1107 58-59 1.1172667172669009 1107 60-61 1.3521956334250014 1107 62-63 1.487794214805497 1107 64-65 1.6507307678026493 1107 66-67 1.6078318794699875 1107 68-69 1.7803615116718667 1107 70-71 1.9207254291764393 1107 72-73 2.0179914463477004 1107 74-75 2.011693460648896 1107 76-77 2.0587442125826954 1107 78-79 2.3946332489141966 1107 80-81 2.498047779774957 1107 82-83 2.6670875386139485 1107 84-85 2.5485954434802345 1107 86-87 2.757451443399905 1107 88-89 2.7868723648577642 1107 90-91 2.897139134279758 1107 92-93 2.5774293198203715 1107 94-95 2.476219392087417 1107 96-97 2.497221644483261 1107 98-99 2.1762130375030377 1107 100-101 1.452093865260613 1108 1 0.13304263606459088 1108 2 0.22250527922075136 1108 3 0.18557315877496094 1108 4 0.12072514553691605 1108 5 0.11606000314542797 1108 6 0.16931964716251713 1108 7 0.12559459596339906 1108 8 0.1366097758542395 1108 9 0.11160022545089276 1108 10-11 0.07747130785946155 1108 12-13 0.16310062712568651 1108 14-15 0.10427866831192745 1108 16-17 0.24232241720689984 1108 18-19 0.3334654981405336 1108 20-21 0.3322961234003188 1108 22-23 0.2533144914096681 1108 24-25 0.3908671621516575 1108 26-27 0.3841067303778871 1108 28-29 0.4595849014274478 1108 30-31 0.35616155594554755 1108 32-33 0.5272267444501892 1108 34-35 0.5263931011190763 1108 36-37 0.5545097102771379 1108 38-39 0.3881089215451041 1108 40-41 0.46222591391115486 1108 42-43 0.48641351965331836 1108 44-45 0.49195174872208014 1108 46-47 0.7334047119674594 1108 48-49 0.830766066400713 1108 50-51 0.7577041674660698 1108 52-53 0.8281417597125618 1108 54-55 0.9368063683926273 1108 56-57 1.1451548247600343 1108 58-59 1.2420903350883918 1108 60-61 1.4025425709378005 1108 62-63 1.6079055976357708 1108 64-65 1.7402782502755585 1108 66-67 1.8395501557573155 1108 68-69 1.991014694900887 1108 70-71 2.085657558455491 1108 72-73 2.125786862843917 1108 74-75 2.2051104482425252 1108 76-77 2.2925506916730356 1108 78-79 2.5744909597813823 1108 80-81 2.6747908321642946 1108 82-83 2.955813225806125 1108 84-85 2.948938536477268 1108 86-87 2.9237913262262722 1108 88-89 3.07513130380746 1108 90-91 3.0003790999434266 1108 92-93 2.9599219957174796 1108 94-95 2.80666468312627 1108 96-97 2.764794986130358 1108 98-99 2.6458804087973604 1108 100-101 1.827760573375965 1109 1 0.6103680389965014 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-0.7985650272722538 2316 48-49 -0.7585950143407238 2316 50-51 -0.7058800738157451 2316 52-53 -0.7379880046606679 2316 54-55 -0.8606167921477414 2316 56-57 -0.8925388227423205 2316 58-59 -0.6916760488261531 2316 60-61 -0.8217620976138811 2316 62-63 -0.7984250841110061 2316 64-65 -0.6524684163911054 2316 66-67 -0.5837931555841855 2316 68-69 -0.5717922168176166 2316 70-71 -0.625697515374231 2316 72-73 -0.30431942681992297 2316 74-75 -0.22862939467296783 2316 76-77 -0.15552194420506993 2316 78-79 -0.19045355796916752 2316 80-81 -0.3429936478859723 2316 82-83 -0.24376885399280823 2316 84-85 -0.12850533408509435 2316 86-87 0.012025821984988028 2316 88-89 0.035107979899194675 2316 90-91 -0.23349173888786368 2316 92-93 -0.23455762727969187 2316 94-95 -0.16973257731985036 2316 96-97 -0.12453065634529281 2316 98-99 -0.2794837885007855 2316 100-101 0.12496346373097644 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11061.0 3 4290.0 4 4838.0 5 5424.0 6 6468.0 7 8121.0 8 10307.0 9 13729.0 10 16838.0 11 20624.0 12 25014.0 13 29278.0 14 32977.0 15 36994.0 16 42197.0 17 47728.0 18 48280.0 19 49351.0 20 54234.0 21 59528.0 22 65632.0 23 73318.0 24 80489.0 25 88405.0 26 97726.0 27 107750.0 28 117837.0 29 130214.0 30 145611.0 31 161933.0 32 181037.0 33 205486.0 34 230305.0 35 250011.0 36 245857.0 37 174103.0 38 44683.0 39 685.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.4809751856508 18.127320742116375 14.346218812362846 24.045485259869977 2 15.56806330742778 19.309332523521494 40.16616628447692 24.956437884573802 3 19.47715035121509 22.917152045628924 29.2581200341889 28.347577568967086 4 13.120835968282895 16.038668931100467 36.282631949903674 34.55786315071296 5 14.26218965451255 36.79319686606416 34.02783473026391 14.916778749159382 6 33.50614392743253 35.30772255955576 16.929174079709274 14.256959433302436 7 29.58525140962594 31.86948597548165 20.021249011425617 18.5240136034668 8 26.823305624982062 34.79131279644815 19.342699876603774 19.042681701966004 9 26.417447632608205 16.730680081755885 19.663055884172557 37.188816401463356 10-11 25.31819387599453 26.075867074362048 27.159331036587876 21.446608013055542 12-13 26.264700165639997 23.220480195447685 27.279582487455734 23.235237151456584 14-15 22.777177818088845 24.922378614054974 25.334247192538783 26.9661963753174 16-17 22.804559139079768 27.966432554622163 26.27320199285572 22.95580631344235 18-19 22.868150788753336 26.50155875346862 28.170269542317598 22.460020915460447 20-21 23.14005586404196 25.93100331347933 28.38723793767297 22.541702884805737 22-23 22.919224465227945 25.818136849994637 28.33017686472482 22.932461820052595 24-25 22.81168150886697 26.02747354394095 28.363529337609055 22.79731560958303 26-27 22.42737456266226 26.322834039091923 28.101120223446852 23.14867117479896 28-29 22.569390950148648 26.809982226324514 27.928480342205596 22.69214648132124 30-31 22.71585972384631 26.245771249312078 27.88379189849427 23.154577128347345 32-33 22.289201486623124 26.220947312327493 27.977533271390705 23.51231792965868 34-35 23.131993107174083 26.433311021091644 27.694894926080092 22.739800945654174 36-37 23.289618867705382 25.856480137189862 27.731140664018067 23.12276033108669 38-39 22.71408354228953 26.228492511475583 27.713952900194734 23.343471046040154 40-41 23.235483319549434 26.041165210890206 27.825512430524807 22.897839039035553 42-43 22.91436517631131 26.116417429741112 27.598860413261573 23.37035698068601 44-45 22.80151986225544 26.375562942778735 27.82986914232563 22.993048052640198 46-47 23.368952084610616 26.61200608655408 27.05497196667082 22.964069862164486 48-49 23.053509968246292 26.681961181714755 27.021101170928674 23.243427679110283 50-51 23.126959293150044 26.80767259112175 27.077375835586555 22.98799228014165 52-53 23.473033666805893 26.380351336389037 27.17413169120894 22.972483305596132 54-55 22.926882994691127 26.626908437680896 27.397028463704466 23.049180103923515 56-57 23.005009578768142 26.743794291877247 27.139393403311818 23.11180272604279 58-59 22.96457890730466 26.310904175120303 27.151976264135257 23.572540653439777 60-61 22.541493710393805 26.583756940662873 27.518450803577508 23.356298545365814 62-63 22.822561241438972 26.559094596812216 27.579254455611068 23.039089706137744 64-65 22.511635340836598 26.60574192288095 27.375989874828733 23.506632861453724 66-67 22.433588041211554 26.65067080018434 27.321428297008815 23.594312861595288 68-69 23.054996709534358 26.19205527572463 27.45433390354411 23.298614111196905 70-71 22.78617909753466 26.216011199786333 27.138784519895232 23.85902518278378 72-73 22.784648439594548 26.38051235848222 27.155002973030296 23.67983622889294 74-75 22.814910596395165 26.661618780050805 27.006944771748774 23.516525851805252 76-77 22.96117218544064 26.228791585465537 27.12815830630139 23.68187792279243 78-79 22.94453068742782 26.409853636995912 27.038828809091502 23.606786866484764 80-81 23.156568356004428 26.44140379661281 26.74473050319481 23.657297344187956 82-83 23.056666248289133 26.443048145007946 26.87355008704814 23.62673551965478 84-85 22.97357131721301 26.417532308639093 26.89348526898864 23.715411105159255 86-87 23.184651297086834 26.424563825749196 26.542209940430766 23.848574936733197 88-89 22.999068929289177 26.429104737599275 26.433920620586292 24.137905712525257 90-91 22.939374914176916 26.436413718850062 26.534226417182516 24.089984949790505 92-93 23.162586637577434 25.970883899669467 26.755514511260582 24.111014951492518 94-95 22.83216258325914 25.70870499403709 26.95173638252658 24.507396040177184 96-97 23.071019346121222 25.376548397580457 26.897873089677056 24.65455916662127 98-99 22.89943698097484 25.56222495829014 26.432402709731846 25.105935351003172 100-101 23.85105857351801 24.574566026316667 26.64674922574154 24.927626174423782 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 139.0 1 116.0 2 118.0 3 157.0 4 154.5 5 164.0 6 258.0 7 548.0 8 901.5 9 1055.5 10 966.5 11 911.0 12 1022.5 13 1134.0 14 1274.5 15 1488.0 16 1716.0 17 1949.5 18 2232.5 19 2598.0 20 3112.5 21 3745.5 22 4633.0 23 5775.0 24 7183.0 25 9140.0 26 12294.0 27 16306.0 28 19712.5 29 23558.5 30 28188.0 31 34884.0 32 43915.5 33 48267.5 34 49777.5 35 53983.5 36 59928.0 37 66376.0 38 71801.5 39 76225.0 40 81685.5 41 87526.5 42 91506.0 43 94938.5 44 98414.5 45 103742.0 46 107613.5 47 110137.0 48 112164.5 49 111325.5 50 109539.0 51 108244.5 52 107494.5 53 107384.0 54 107205.5 55 102534.0 56 95510.5 57 88161.0 58 81535.0 59 72485.5 60 59862.5 61 49986.0 62 42648.0 63 34004.0 64 25478.5 65 19473.0 66 14672.0 67 11090.0 68 8459.5 69 6505.5 70 5021.5 71 3732.0 72 2778.5 73 2175.0 74 1668.0 75 1198.0 76 897.5 77 682.5 78 476.5 79 289.0 80 166.5 81 103.0 82 69.5 83 43.0 84 27.0 85 20.5 86 14.0 87 8.5 88 5.5 89 4.0 90 2.5 91 3.0 92 3.5 93 2.0 94 2.0 95 1.5 96 0.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3914132230191407 2 0.03189495291396592 3 0.03746120272657454 4 0.0465106272685456 5 0.06648765880459492 6 0.05641377110692903 7 0.03988576552838566 8 0.041217567630788945 9 0.03797343430442196 10-11 0.04021017886102236 12-13 0.03175835782653995 14-15 0.029077679235805128 16-17 0.02407488415882867 18-19 0.029487464498083062 20-21 0.0367953016753729 22-23 0.035548871502610845 24-25 0.044137287624519225 26-27 0.03874178167119309 28-29 0.03414877185649457 30-31 0.03688067360501413 32-33 0.03151931642354449 34-35 0.03317553185858447 36-37 0.03527568132775889 38-39 0.017637840663879446 40-41 0.014666897512364416 42-43 0.015691360668059255 44-45 0.011422764185997433 46-47 0.02042096557018375 48-49 0.017620766277951196 50-51 0.011269094712643207 52-53 0.009766548750957447 54-55 0.008468895420410653 56-57 0.00930554033089477 58-59 0.0046442329724832605 60-61 0.008144482087773953 62-63 0.011661805588992893 64-65 0.007341985949146332 66-67 0.0036368442027166718 68-69 0.007034647002437881 70-71 0.015366947335422555 72-73 0.012771640674328968 74-75 0.013830252601880299 76-77 0.006539489810518709 78-79 0.009100647699755802 80-81 0.011337392256356197 82-83 0.013164351550678657 84-85 0.00811033331591746 86-87 0.003056315081156264 88-89 0.018815973292928504 90-91 0.028309331869033994 92-93 0.012327706640194539 94-95 0.023272388020201047 96-97 0.02112101539324189 98-99 0.013591211198884836 100-101 0.12944091972204264 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2928363.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.178661006395956 #Duplication Level Percentage of deduplicated Percentage of total 1 76.07657645054024 41.97803622491526 2 13.421936631795106 14.812089829103003 3 4.507565155404379 7.461642290229023 4 2.0635086273947816 4.554465721391603 5 1.0815551153547693 2.983938153494715 6 0.6605514495427659 2.1869006706962244 7 0.43368500354644557 1.6751110455972864 8 0.30448966693590745 1.344106568944548 9 0.2193485821234271 1.089302494969997 >10 1.1132953823986924 11.043656928296546 >50 0.0749742391946847 2.8315514834570776 >100 0.037281503002842276 3.7969478264025787 >500 0.0023669443465970455 0.9358179029834789 >1k 0.0028029604102847807 3.1151999077053203 >5k 6.228800911743958E-5 0.1912329518135043 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5564 0.19000376660953577 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4486 0.1531913905482346 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4104 0.1401465596990537 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4018 0.13720976531939516 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3634 0.12409663692650125 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3510 0.11986218921629593 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3217 0.10985659906234302 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3004 0.10258291065690969 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2947 0.10063643066108949 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 3.4148771856494564E-5 6 0.0 0.0 0.0 0.0 6.829754371298913E-5 7 0.0 0.0 0.0 0.0 6.829754371298913E-5 8 0.0 0.0 0.0 0.0 6.829754371298913E-5 9 0.0 0.0 0.0 3.4148771856494564E-5 6.829754371298913E-5 10-11 0.0 0.0 0.0 6.829754371298913E-5 6.829754371298913E-5 12-13 0.0 0.0 0.0 6.829754371298913E-5 2.048926311389674E-4 14-15 3.4148771856494564E-5 0.0 0.0 6.829754371298913E-5 3.073389467084511E-4 16-17 3.4148771856494564E-5 3.4148771856494564E-5 0.0 6.829754371298913E-5 4.6100842006267665E-4 18-19 3.4148771856494564E-5 3.4148771856494564E-5 0.0 6.829754371298913E-5 5.46380349703913E-4 20-21 3.4148771856494564E-5 3.4148771856494564E-5 0.0 8.537192964123641E-5 7.341985949146331E-4 22-23 3.4148771856494564E-5 3.4148771856494564E-5 0.0 1.7074385928247284E-4 7.854217526993751E-4 24-25 3.4148771856494564E-5 3.4148771856494564E-5 0.0 3.7563649042144027E-4 7.854217526993751E-4 26-27 3.4148771856494564E-5 3.4148771856494564E-5 0.0 8.537192964123642E-4 8.537192964123641E-4 28-29 3.4148771856494564E-5 3.4148771856494564E-5 0.0 0.0033636540278647147 8.537192964123641E-4 30-31 3.4148771856494564E-5 3.4148771856494564E-5 0.0 0.012959458919539688 8.707936823406114E-4 32-33 3.4148771856494564E-5 3.4148771856494564E-5 0.0 0.03249255642145458 9.220168401253533E-4 34-35 3.4148771856494564E-5 3.4148771856494564E-5 0.0 0.06303863284708897 0.001024463155694837 36-37 3.4148771856494564E-5 3.4148771856494564E-5 0.0 0.11328855063392074 0.001024463155694837 38-39 3.4148771856494564E-5 3.4148771856494564E-5 0.0 0.1862986248631061 0.0010586119275513316 40-41 3.4148771856494564E-5 3.4148771856494564E-5 0.0 0.26907524784324893 0.0010586119275513316 42-43 3.4148771856494564E-5 3.4148771856494564E-5 0.0 0.35222750731381325 0.0010756863134795788 44-45 3.4148771856494564E-5 3.4148771856494564E-5 0.0 0.4594887997150626 0.0011781326290490625 46-47 3.4148771856494564E-5 3.4148771856494564E-5 0.0 0.5863002640041552 0.001263504558690299 48-49 3.4148771856494564E-5 3.4148771856494564E-5 0.0 0.7185926061762151 0.0014342484179727718 50-51 3.4148771856494564E-5 3.4148771856494564E-5 0.0 0.8660811518244151 0.0014683971898292664 52-53 3.4148771856494564E-5 3.4148771856494564E-5 0.0 1.0049471325788504 0.0016049922772552446 54-55 3.4148771856494564E-5 3.4148771856494564E-5 0.0 1.1585653827752913 0.0016391410491117392 56-57 3.4148771856494564E-5 3.4148771856494564E-5 0.0 1.3438907676404872 0.0017074385928247285 58-59 3.4148771856494564E-5 3.4148771856494564E-5 0.0 1.541629231075519 0.0017586617506094703 60-61 3.4148771856494564E-5 3.4148771856494564E-5 0.0 1.751524657291463 0.0017928105224659647 62-63 1.024463155694837E-4 3.4148771856494564E-5 0.0 1.9575954210594793 0.0018440336802507066 64-65 1.024463155694837E-4 3.4148771856494564E-5 0.0 2.177564734973089 0.0018781824521072012 66-67 1.024463155694837E-4 5.122315778474185E-5 0.0 2.432707283898888 0.0018781824521072012 68-69 1.024463155694837E-4 6.829754371298913E-5 0.0 2.7146053955742513 0.0018952568380354484 70-71 1.024463155694837E-4 6.829754371298913E-5 0.0 3.0135437444060043 0.0019294056098919429 72-73 1.024463155694837E-4 6.829754371298913E-5 0.0 3.3182532356815053 0.001997703153604932 74-75 1.024463155694837E-4 6.829754371298913E-5 0.0 3.620999172575258 0.002117223855102663 76-77 1.024463155694837E-4 6.829754371298913E-5 0.0 3.9550936820332723 0.002185521398815652 78-79 1.024463155694837E-4 6.829754371298913E-5 0.0 4.331959528241547 0.0022196701706721465 80-81 1.024463155694837E-4 6.829754371298913E-5 0.0 4.725063115467584 0.0022879677143851362 82-83 1.024463155694837E-4 6.829754371298913E-5 0.0 5.134028807220963 0.0023904140299546195 84-85 1.024463155694837E-4 6.829754371298913E-5 0.0 5.535874480042263 0.002458711573667609 86-87 1.024463155694837E-4 6.829754371298913E-5 0.0 5.9521821577447875 0.0025099347314523506 88-89 1.024463155694837E-4 6.829754371298913E-5 0.0 6.390003561716904 0.0025611578892370925 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4160 0.0 47.480457 1 GTATCAA 7280 0.0 40.435394 1 ATCAACG 9285 0.0 31.123856 3 TATCAAC 9550 0.0 30.359747 2 TCAACGC 9595 0.0 30.167828 4 CAACGCA 9795 0.0 29.454288 5 AACGCAG 10180 0.0 28.52028 6 CGCAGAG 11520 0.0 25.113884 8 ACGCAGA 11560 0.0 25.026985 7 TAAGGTG 1805 0.0 25.015585 5 GTAAGGT 1735 0.0 24.381601 4 GTACATG 9160 0.0 23.646578 1 TACATGG 8980 0.0 23.130062 2 AGGTAAG 1795 0.0 22.507444 2 GGTAAGG 1910 0.0 21.898836 3 GCAGAGT 13175 0.0 21.489307 9 ACATGGG 9355 0.0 21.237537 3 GAGTACT 7395 0.0 20.71553 12-13 AGAGTAC 11340 0.0 20.441082 10-11 GGACCGA 745 0.0 19.772692 6 >>END_MODULE