FastQCFastQC Report
Thu 26 May 2016
SRR938468_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938468_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2928363
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53450.18252518557296346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45460.1552403168596243No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA40550.13847326987808548No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA40190.13724391409125167No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA39820.13598040953256138No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC37200.1270334313061598No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA36990.1263163070971734No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG32570.1112225499366028No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30030.10254876188505319No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46000.043.384241
GTATCAA78150.038.3655051
ATCAACG95950.030.9394363
TCAACGC96750.030.7321744
TATCAAC97700.030.4866472
CAACGCA98950.030.0488935
AACGCAG102850.028.9094586
TAAGGTG17800.026.41715
ACGCAGA116700.025.3970747
CGCAGAG118650.024.9796758
TACATGG83950.023.7665042
GTACATG88950.023.5577141
GTAAGGT19350.022.5827484
ACATGGG85950.022.2155743
GCAGAGT132350.021.7838549
AGAGTAC110700.020.93833510-11
GGTAAGG19800.020.6305433
TGGTATC26000.020.4635682
AGGTAAG19800.020.1535152
GAGTACT76050.019.95453512-13
Produced by FastQC (version 0.11.5)