##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938468_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2928363 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82875108038177 31.0 31.0 34.0 30.0 34.0 2 32.087522619292756 33.0 31.0 34.0 30.0 34.0 3 32.148901280339906 33.0 31.0 34.0 30.0 34.0 4 35.79731611142471 37.0 35.0 37.0 35.0 37.0 5 35.686306308336775 37.0 35.0 37.0 33.0 37.0 6 35.71590987865917 37.0 35.0 37.0 33.0 37.0 7 35.63753298344502 37.0 35.0 37.0 33.0 37.0 8 35.59904151227153 37.0 35.0 37.0 33.0 37.0 9 37.15373777089794 39.0 37.0 39.0 33.0 39.0 10-11 37.08378401174991 39.0 37.0 39.0 33.0 39.0 12-13 36.97527492322502 39.0 37.0 39.0 33.0 39.0 14-15 38.01594115893419 40.0 37.0 41.0 33.0 41.0 16-17 38.094398133018345 40.0 37.0 41.0 33.0 41.0 18-19 38.21747286794704 40.0 37.5 41.0 33.0 41.0 20-21 38.15934978006484 40.0 37.0 41.0 33.0 41.0 22-23 38.04178204682958 40.0 37.0 41.0 33.0 41.0 24-25 37.925841844061 40.0 37.0 41.0 33.0 41.0 26-27 37.71890916529132 40.0 37.0 41.0 32.0 41.0 28-29 37.58700868027631 39.5 37.0 41.0 32.0 41.0 30-31 37.33496735206667 39.0 36.0 41.0 31.5 41.0 32-33 37.24726579320938 39.0 36.0 41.0 31.0 41.0 34-35 37.13714112628796 39.0 36.0 41.0 31.0 41.0 36-37 37.021033765281146 39.0 36.0 41.0 31.0 41.0 38-39 36.80717998417546 39.0 35.5 41.0 30.0 41.0 40-41 36.59627819365291 39.0 35.0 40.0 30.0 41.0 42-43 36.341704221778514 39.0 35.0 40.0 30.0 41.0 44-45 36.07623627945033 38.0 35.0 40.0 29.5 41.0 46-47 35.71218834550225 38.0 35.0 40.0 28.5 41.0 48-49 35.3193125305845 38.0 34.0 40.0 27.0 41.0 50-51 34.9323663084119 38.0 34.0 40.0 26.0 41.0 52-53 34.33734717997734 38.0 33.0 40.0 24.0 41.0 54-55 33.92175560202065 37.0 33.0 40.0 23.5 41.0 56-57 33.53022661466492 37.0 32.0 39.5 23.0 41.0 58-59 32.99913108450011 36.0 31.5 39.0 21.5 40.0 60-61 32.51109237481829 36.0 31.0 39.0 20.0 40.0 62-63 32.00729622659486 35.0 30.5 39.0 18.0 40.0 64-65 31.359120607656905 35.0 29.5 38.0 14.0 40.0 66-67 30.726141192195094 34.0 29.0 38.0 9.5 40.0 68-69 30.011935166507705 34.0 28.0 37.0 7.5 39.0 70-71 29.250401674928963 33.5 27.0 36.0 4.5 39.0 72-73 28.68006032722036 33.0 26.0 36.0 2.0 39.0 74-75 27.9788632761717 32.5 26.0 35.0 2.0 38.0 76-77 26.83602835440825 31.0 25.0 34.0 2.0 36.0 78-79 27.34155072304902 32.0 26.0 35.0 2.0 37.0 80-81 26.99166855338631 32.0 25.0 35.0 2.0 36.0 82-83 26.333996673226643 32.0 23.5 35.0 2.0 36.0 84-85 25.580555416114738 31.0 21.0 34.0 2.0 35.0 86-87 24.720023439717004 31.0 19.0 34.0 2.0 35.0 88-89 23.779504282768222 30.0 11.5 34.0 2.0 35.0 90-91 22.951957117338253 30.0 4.5 34.0 2.0 35.0 92-93 21.918201227101967 29.0 2.0 34.0 2.0 35.0 94-95 20.784890739297005 28.5 2.0 33.5 2.0 35.0 96-97 19.45741409108092 26.5 2.0 33.0 2.0 34.0 98-99 17.718799889221387 22.5 2.0 32.5 2.0 34.0 100-101 13.385518120533554 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2647519869848658 1101 2 0.2820905852967286 1101 3 0.31556845643071796 1101 4 0.15191021542728578 1101 5 0.09255985519351384 1101 6 0.25455965059947516 1101 7 0.24171650957472934 1101 8 0.2393937536330739 1101 9 0.23855683230743807 1101 10-11 0.33000364780800595 1101 12-13 0.31982180554054196 1101 14-15 0.47051473694730817 1101 16-17 0.4111706665895625 1101 18-19 0.3473438656498473 1101 20-21 0.371326679167332 1101 22-23 0.3878291125435922 1101 24-25 0.18912626744534577 1101 26-27 0.33370303926356115 1101 28-29 0.42587883555552963 1101 30-31 0.4178454142611159 1101 32-33 0.38614316906338786 1101 34-35 0.28521905126786606 1101 36-37 0.3692325270743879 1101 38-39 0.5399670170236774 1101 40-41 0.44938571572592423 1101 42-43 0.4510206916545414 1101 44-45 0.5795107532854473 1101 46-47 0.6474982763979824 1101 48-49 0.6410412212041336 1101 50-51 0.6667080362610847 1101 52-53 0.7778149059995414 1101 54-55 0.8108036101977234 1101 56-57 0.8395563206263787 1101 58-59 0.9158611859104084 1101 60-61 1.0188357768166583 1101 62-63 1.1936946227980236 1101 64-65 1.1575773158884317 1101 66-67 1.2386396849541725 1101 68-69 1.322181642769312 1101 70-71 1.6059947558687213 1101 72-73 1.8518375120848454 1101 74-75 1.849327714454823 1101 76-77 1.7094591042077063 1101 78-79 1.8991080747746913 1101 80-81 2.1130326086189086 1101 82-83 2.123196658746064 1101 84-85 2.258794061143984 1101 86-87 2.2017035614163163 1101 88-89 1.957645590918144 1101 90-91 2.260989707969994 1101 92-93 2.54186653804641 1101 94-95 2.544809062728543 1101 96-97 2.5488178810556157 1101 98-99 2.484723718684137 1101 100-101 2.2832811668826096 1102 1 0.19255817812297593 1102 2 0.20969688010502807 1102 3 0.2196672812198983 1102 4 0.2280388325780507 1102 5 0.16916673993024745 1102 6 0.3007354425032318 1102 7 0.19888780992769028 1102 8 0.31018750734127565 1102 9 0.32734607090934276 1102 10-11 0.28660681832516843 1102 12-13 0.37838232031523233 1102 14-15 0.46096194381905775 1102 16-17 0.46669668740454995 1102 18-19 0.5137206652861863 1102 20-21 0.40177891988675896 1102 22-23 0.5009330197229716 1102 24-25 0.39098616224209337 1102 26-27 0.4627617578994503 1102 28-29 0.41072458547454715 1102 30-31 0.43930277563216436 1102 32-33 0.38983545989745494 1102 34-35 0.5348220241010964 1102 36-37 0.6230037157925352 1102 38-39 0.5445866914815696 1102 40-41 0.549071061203648 1102 42-43 0.5642264991114345 1102 44-45 0.6023088906576888 1102 46-47 0.5480801751346149 1102 48-49 0.5940208803210254 1102 50-51 0.6785414825349747 1102 52-53 0.5373985099715952 1102 54-55 0.4869958271768624 1102 56-57 0.8146031810892325 1102 58-59 0.8292142607415229 1102 60-61 0.880538034617075 1102 62-63 0.9768280576325346 1102 64-65 1.108178846370734 1102 66-67 1.2193891868726432 1102 68-69 1.4335838283285476 1102 70-71 1.4056209393207197 1102 72-73 1.5171755275385514 1102 74-75 1.5023810202729102 1102 76-77 1.5051480717652872 1102 78-79 1.7997815926624803 1102 80-81 1.9883857295198801 1102 82-83 2.0029844589012704 1102 84-85 2.0147946925873406 1102 86-87 1.9028338215951877 1102 88-89 1.7485323614901276 1102 90-91 1.9489343585502965 1102 92-93 2.268223689018761 1102 94-95 2.061368118103861 1102 96-97 2.2704444125042116 1102 98-99 2.2916767012714203 1102 100-101 2.0687889562673583 1103 1 0.2301001561829814 1103 2 0.24120299530937217 1103 3 0.18227103499018682 1103 4 0.17808726735029978 1103 5 0.1755090294213133 1103 6 0.1855935909872386 1103 7 0.19201175717552843 1103 8 0.2146682770266395 1103 9 0.33298120266650955 1103 10-11 0.33812383764023224 1103 12-13 0.32795740955241115 1103 14-15 0.4253578596059242 1103 16-17 0.3861435753824125 1103 18-19 0.30091742614163763 1103 20-21 0.3288884227085944 1103 22-23 0.5046879090950398 1103 24-25 0.35587598193698966 1103 26-27 0.45440443351071025 1103 28-29 0.4340369002291524 1103 30-31 0.41865129273849533 1103 32-33 0.39950719989275285 1103 34-35 0.5855079696177654 1103 36-37 0.5109912635797471 1103 38-39 0.41585694996633293 1103 40-41 0.4828522275713141 1103 42-43 0.42377370691973937 1103 44-45 0.5452997680211453 1103 46-47 0.6801725623251045 1103 48-49 0.6710136216436311 1103 50-51 0.722839872418291 1103 52-53 0.5799518272002473 1103 54-55 0.7308452272244423 1103 56-57 0.7645178921328224 1103 58-59 0.9404642928050393 1103 60-61 0.8640301640923198 1103 62-63 0.9485785898869246 1103 64-65 1.0876244342044004 1103 66-67 0.9332843459605016 1103 68-69 0.851744277690571 1103 70-71 1.0021332585013383 1103 72-73 1.0079192301379827 1103 74-75 1.0917320471353484 1103 76-77 1.1513548822072828 1103 78-79 1.2375082691569332 1103 80-81 1.356731669473838 1103 82-83 1.4909969156406717 1103 84-85 1.6667100036587854 1103 86-87 1.5909501641311259 1103 88-89 1.3432826960823618 1103 90-91 1.7182839997658235 1103 92-93 1.7301986206291673 1103 94-95 1.6292880156414355 1103 96-97 1.6293314890708857 1103 98-99 1.6036254288150111 1103 100-101 1.4729901446544815 1104 1 0.17144896925983844 1104 2 0.1928599752361606 1104 3 0.22606342935236512 1104 4 0.17215598370255947 1104 5 0.1513502525685695 1104 6 0.11313130263179971 1104 7 0.17440021686488905 1104 8 0.1328172290975047 1104 9 0.18988734839125954 1104 10-11 0.2033029828270898 1104 12-13 0.29093799799210984 1104 14-15 0.23153976087257888 1104 16-17 0.1759848746907764 1104 18-19 0.36307690272447246 1104 20-21 0.31315365849859944 1104 22-23 0.3019595566976321 1104 24-25 0.2687236454572499 1104 26-27 0.4500669398772956 1104 28-29 0.34744625186018396 1104 30-31 0.4208660337193564 1104 32-33 0.6369037278986198 1104 34-35 0.500355219012043 1104 36-37 0.5200846608884007 1104 38-39 0.5680125470931543 1104 40-41 0.5701138076285304 1104 42-43 0.6030229012781021 1104 44-45 0.5316196874807844 1104 46-47 0.6336524105741148 1104 48-49 0.733857358107187 1104 50-51 0.6461362438178995 1104 52-53 0.6800722569392477 1104 54-55 0.8595035780510258 1104 56-57 0.9022672602336925 1104 58-59 0.8952578010232912 1104 60-61 0.8946134867154996 1104 62-63 0.9687822256274252 1104 64-65 0.980421855199161 1104 66-67 0.903967439624374 1104 68-69 1.0777283028998959 1104 70-71 1.1493022076209627 1104 72-73 1.3378764847207414 1104 74-75 1.3428898148535175 1104 76-77 1.2765812234756488 1104 78-79 1.4769679299544336 1104 80-81 1.5553224511247272 1104 82-83 1.6747494920278712 1104 84-85 1.9162975437028642 1104 86-87 1.7822905280859835 1104 88-89 1.6929721015973804 1104 90-91 1.9914695805656102 1104 92-93 2.0307662588258637 1104 94-95 2.0425058935075917 1104 96-97 2.318304075835041 1104 98-99 2.4194026766384695 1104 100-101 2.655360159077489 1105 1 0.07374649134442635 1105 2 0.09181727459390743 1105 3 0.12083338253167852 1105 4 0.12356060412870562 1105 5 0.1008342279299228 1105 6 0.12457268159829482 1105 7 0.1482370651486562 1105 8 0.12434894070386804 1105 9 0.2804271233075184 1105 10-11 0.19399810903443182 1105 12-13 0.17035004264625542 1105 14-15 0.2753678614395909 1105 16-17 0.262042351269109 1105 18-19 0.25171812849261954 1105 20-21 0.2218444924324885 1105 22-23 0.2727974791828913 1105 24-25 0.2753737231895528 1105 26-27 0.2512060693661695 1105 28-29 0.254982545819729 1105 30-31 0.3527399034282297 1105 32-33 0.35477399880588933 1105 34-35 0.3544287995476907 1105 36-37 0.35246775649233797 1105 38-39 0.2401323726827158 1105 40-41 0.3588343520719164 1105 42-43 0.3545978302312349 1105 44-45 0.3767112212320143 1105 46-47 0.38713620010314287 1105 48-49 0.43903845397400687 1105 50-51 0.508542251187734 1105 52-53 0.42421355296107777 1105 54-55 0.4158874849716909 1105 56-57 0.6110522567663352 1105 58-59 0.731592666340191 1105 60-61 0.7535779700789789 1105 62-63 0.9276907326686548 1105 64-65 1.0381132738250045 1105 66-67 1.0474920896023896 1105 68-69 1.1050170999302047 1105 70-71 1.21100488496619 1105 72-73 1.3851606132804477 1105 74-75 1.3552129908639223 1105 76-77 1.1417079747186882 1105 78-79 1.2915063632519583 1105 80-81 1.5829634230731102 1105 82-83 1.652998838215364 1105 84-85 1.6834172333175559 1105 86-87 1.6738889806919417 1105 88-89 1.6187665347861255 1105 90-91 1.7244017880270057 1105 92-93 1.823307357230874 1105 94-95 1.6857122489731005 1105 96-97 1.989382220593889 1105 98-99 1.9782545274831023 1105 100-101 1.798861693327293 1106 1 0.017105148912733625 1106 2 0.063803443393887 1106 3 0.057033175558160565 1106 4 0.05363949071004015 1106 5 0.07230075543692038 1106 6 0.027295125157394295 1106 7 0.09143987447871638 1106 8 0.043869440265901005 1106 9 0.03466328151158393 1106 10-11 0.07103760177270146 1106 12-13 0.10240639171805554 1106 14-15 0.1550883412225943 1106 16-17 0.07203443082322991 1106 18-19 0.14729384866159023 1106 20-21 0.11446880899602974 1106 22-23 0.1849535976169534 1106 24-25 0.07003130739175134 1106 26-27 -0.15424261462128896 1106 28-29 0.19509620465954924 1106 30-31 0.35248626373169856 1106 32-33 0.10823047432226929 1106 34-35 0.07250684198785962 1106 36-37 0.09515988690684907 1106 38-39 0.12682783374626894 1106 40-41 0.21630870995951312 1106 42-43 0.20176368807644707 1106 44-45 0.1819806135210129 1106 46-47 -0.15264726355994185 1106 48-49 0.007179012308043298 1106 50-51 0.2162347524592434 1106 52-53 0.21458420687026347 1106 54-55 0.11427787730308125 1106 56-57 0.46044590557534804 1106 58-59 0.5034699179719553 1106 60-61 0.6059510575164566 1106 62-63 0.5198376591471465 1106 64-65 0.6242123615167898 1106 66-67 0.6874517544889436 1106 68-69 0.5941443674558471 1106 70-71 0.36183375035569654 1106 72-73 0.46583503439033436 1106 74-75 0.4294117631506609 1106 76-77 0.6755636866228407 1106 78-79 0.9309459453491051 1106 80-81 0.8830536207928681 1106 82-83 1.1788685420242437 1106 84-85 1.2103184935249516 1106 86-87 1.1982973313319327 1106 88-89 1.0713551166289683 1106 90-91 1.2082823120302812 1106 92-93 1.1636494463213225 1106 94-95 1.0245510427342275 1106 96-97 1.1737643728359828 1106 98-99 1.2000340759558767 1106 100-101 1.3195186488385815 1107 1 0.07084033394724543 1107 2 0.057995264990509554 1107 3 0.09110018022300892 1107 4 0.07644949471041684 1107 5 -0.05082173585039129 1107 6 0.0041912373307511075 1107 7 0.008226679667004078 1107 8 0.09187399663057505 1107 9 0.10519306908464188 1107 10-11 0.09130658069273068 1107 12-13 0.0938034565242063 1107 14-15 0.1428710799232107 1107 16-17 0.11294435193438801 1107 18-19 0.05299580935616177 1107 20-21 0.06388106253195502 1107 22-23 0.07143784442681778 1107 24-25 0.12491310549462042 1107 26-27 0.15570693143732228 1107 28-29 0.16482977259142473 1107 30-31 0.13198874617071255 1107 32-33 0.21758216702529865 1107 34-35 0.26063317262263297 1107 36-37 0.3354777179213215 1107 38-39 0.2759295917097333 1107 40-41 0.2797520198329977 1107 42-43 0.3619562233001332 1107 44-45 0.380173471656704 1107 46-47 0.3941670315256971 1107 48-49 0.36926337963782174 1107 50-51 0.27486478768292955 1107 52-53 0.30520456659932194 1107 54-55 0.456474975671739 1107 56-57 0.47466060222452455 1107 58-59 0.4975539962912592 1107 60-61 0.6112724312400672 1107 62-63 0.81524472320746 1107 64-65 0.8767650402456155 1107 66-67 0.785077122320466 1107 68-69 0.9443138326932257 1107 70-71 0.8221149380616488 1107 72-73 1.103830865350563 1107 74-75 0.9567164734282478 1107 76-77 1.121664502971516 1107 78-79 1.2855537555819403 1107 80-81 1.3811162547038833 1107 82-83 1.5527100634228859 1107 84-85 1.4498454655765372 1107 86-87 1.7802497073201629 1107 88-89 1.813421312742495 1107 90-91 1.880505226408598 1107 92-93 1.833315762107226 1107 94-95 1.5821738849023 1107 96-97 1.834163071695535 1107 98-99 1.8245560644836019 1107 100-101 1.6340950111712793 1108 1 -0.02643462521979245 1108 2 0.020062509175531318 1108 3 0.06599399049255084 1108 4 0.02924574919093459 1108 5 0.06923708524086436 1108 6 0.007738103479731251 1108 7 -0.01633587150560345 1108 8 0.04683399264188637 1108 9 0.04307680732357255 1108 10-11 0.09016133548771421 1108 12-13 0.039109062488968505 1108 14-15 0.11034557546567214 1108 16-17 0.07895988377809005 1108 18-19 -0.03225077127744669 1108 20-21 0.07081680926005873 1108 22-23 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0.3239911488385818 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 20.0 4 49.0 5 82.0 6 174.0 7 522.0 8 1277.0 9 2277.0 10 3870.0 11 5883.0 12 8576.0 13 11860.0 14 15912.0 15 20964.0 16 26428.0 17 32120.0 18 33161.0 19 32496.0 20 34365.0 21 36949.0 22 41015.0 23 46403.0 24 51592.0 25 58859.0 26 68511.0 27 80953.0 28 94803.0 29 111068.0 30 129497.0 31 150806.0 32 179139.0 33 214868.0 34 259892.0 35 320591.0 36 378118.0 37 347763.0 38 124174.0 39 3317.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.26236059638276 18.92183150308268 14.892763820811913 23.923044079722644 2 16.044990467889665 20.010430334993398 39.033120924262924 24.911458272854013 3 19.5468332419273 23.075470422350516 29.71865154357814 27.659044792144044 4 13.617471604442482 16.6567122996705 35.97866794519668 33.747148150690336 5 14.24170608021582 36.90047296258985 34.17743780627316 14.680383150921166 6 33.512477790492504 35.750076066389305 16.5980788583929 14.139367284725287 7 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27.927051902159437 22.609938818914095 30-31 22.728927390490863 26.307172300701794 27.89664396114826 23.067256347659086 32-33 22.316604122934717 26.29017149924992 27.95805557462203 23.43516880319333 34-35 23.15999275292901 26.494909035085968 27.69504721439428 22.650050997590743 36-37 23.351113691274023 25.912834128725464 27.78192311304244 22.954129066958075 38-39 22.684366381152696 26.31393262521497 27.74428025735704 23.25742073627529 40-41 23.19565042172016 26.081571498438528 27.88199349182106 22.840784588020252 42-43 22.933973907573442 26.180944919858778 27.633762788546978 23.2513183840208 44-45 22.832540933992522 26.368756386932198 27.913896683874924 22.884805995200356 46-47 23.351799863473115 26.663789061526227 27.13277154012091 22.85163953487975 48-49 23.03370517931008 26.715386719474328 27.085900894117295 23.165007207098302 50-51 23.175644549531597 26.83596603289961 27.150800635030564 22.837588782538234 52-53 23.51600535862528 26.39993743944996 27.197003923352398 22.887053278572363 54-55 22.94807713387992 26.66694668659589 27.361840045103698 23.023136134420493 56-57 23.020762794776466 26.788960248439146 27.137533837164312 23.052743119620075 58-59 22.96084877455425 26.42425136501178 27.109565993013845 23.505333867420124 60-61 22.542389724224762 26.671164059920166 27.524951653876244 23.261494561978825 62-63 22.786382699139416 26.718033249293207 27.544040817344023 22.951543234223355 64-65 22.44334329738432 26.752187785622706 27.324316797743993 23.480152119248977 66-67 22.41444664363662 26.785426458877154 27.26282873483616 23.537298162650067 68-69 23.04300247427588 26.326186089245702 27.32795108750495 23.302860348973468 70-71 22.713124265374294 26.446593692992877 27.02976954351955 23.81051249811328 72-73 22.76575226312912 26.586119793454145 27.00005497963534 23.648072963781395 74-75 22.71192130210633 26.837929587281355 26.83156084133012 23.61858826928219 76-77 22.73352040030556 26.38766095596755 27.229667223633136 23.64915142009375 78-79 22.768027734266553 26.632166162460052 27.122371782460032 23.47743432081337 80-81 22.92359745632194 26.62251336755957 26.870092386245126 23.583796789873368 82-83 22.8324086314013 26.71289550259175 26.973624224857097 23.481071641149857 84-85 22.66287457142989 26.76992476427043 26.94593754210143 23.621263122198254 86-87 22.828471895661878 26.812508089305247 26.633015156839463 23.726004858193413 88-89 22.627966546497138 26.854969824437745 26.411274831706315 24.105788797358798 90-91 22.51894685114996 26.83313965023667 26.67513112453783 23.97278237407554 92-93 22.6668096370459 26.49018020585344 26.844236788316135 23.998773368784523 94-95 22.32359290340349 26.204244250581766 27.023917641315805 24.448245204698942 96-97 22.541317886702895 25.86657218838173 27.04552458925423 24.546585335661142 98-99 22.266416424466502 26.13378191160044 26.68270634480766 24.917095319125394 100-101 22.522619738929002 25.805753765630975 26.924041626705453 24.74758486873457 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 164.0 1 122.5 2 123.0 3 178.0 4 160.0 5 137.0 6 251.5 7 626.0 8 1005.0 9 1130.0 10 1007.5 11 902.0 12 993.5 13 1100.0 14 1280.5 15 1492.5 16 1667.5 17 1891.5 18 2186.5 19 2522.5 20 2920.5 21 3542.0 22 4387.0 23 5466.0 24 6908.0 25 9036.5 26 12103.0 27 15782.0 28 19547.5 29 23823.5 30 28400.5 31 35184.5 32 45373.5 33 49851.5 34 49867.5 35 53636.5 36 60324.5 37 67303.5 38 72607.5 39 77234.0 40 82980.5 41 88847.5 42 92495.0 43 96249.5 44 100106.5 45 105699.0 46 109374.0 47 111682.5 48 113719.0 49 112233.0 50 110897.0 51 109719.5 52 108467.0 53 109038.0 54 109972.5 55 104167.5 56 95366.5 57 87665.5 58 80784.0 59 71260.5 60 58044.5 61 48001.5 62 40594.5 63 31381.5 64 22196.0 65 16200.0 66 12128.0 67 9293.0 68 7134.5 69 5429.5 70 4133.0 71 2919.0 72 2113.0 73 1713.0 74 1296.0 75 945.0 76 701.0 77 478.5 78 312.0 79 206.0 80 117.5 81 65.5 82 40.0 83 17.5 84 8.0 85 4.5 86 3.5 87 2.0 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024348074333680626 2 0.012464301727620516 3 0.001263504558690299 4 0.0 5 4.439340341344294E-4 6 0.0 7 0.0 8 6.829754371298913E-5 9 4.097852622779348E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0017074385928247283 26-27 0.00804203577220447 28-29 2.3904140299546196E-4 30-31 0.0 32-33 4.097852622779348E-4 34-35 0.009681176821316211 36-37 0.0012976533305467935 38-39 0.01258382242911825 40-41 0.012122814009055571 42-43 0.005361357181469647 44-45 7.000498230581387E-4 46-47 6.829754371298913E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 8.537192964123642E-5 66-67 5.122315778474185E-4 68-69 0.0010756863134795788 70-71 3.4148771856494564E-5 72-73 2.048926311389674E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.7074385928247284E-4 82-83 0.001092760699407826 84-85 0.005685770514106346 86-87 0.0038929599916403806 88-89 0.0 90-91 0.0013659508742597827 92-93 5.97603507488655E-4 94-95 5.122315778474185E-5 96-97 1.7074385928247282E-5 98-99 0.0 100-101 8.537192964123642E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2928363.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.84351521848338 #Duplication Level Percentage of deduplicated Percentage of total 1 73.42375648105126 38.06545636523598 2 14.566219136155873 15.103280069221217 3 5.132862005236044 7.9831682844849 4 2.3263946581592276 4.824339074579054 5 1.2851127737222012 3.3312381820967163 6 0.7581575504338564 2.3583331502355427 7 0.49606680927810215 1.8002493023335648 8 0.347180219440989 1.4399234392116247 9 0.2536710113703136 1.1836077244618433 >10 1.2726588669758716 12.000706231376656 >50 0.08856229182529832 3.1593562296670465 >100 0.04328359257199922 4.185414564684688 >500 0.002839216983823377 1.0239636518925823 >1k 0.003169358493250061 3.3579969361834507 >5k 6.602830194270962E-5 0.18296679433520438 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5345 0.18252518557296346 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4546 0.1552403168596243 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4055 0.13847326987808548 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4019 0.13724391409125167 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3982 0.13598040953256138 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3720 0.1270334313061598 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3699 0.1263163070971734 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3257 0.1112225499366028 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3003 0.10254876188505319 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.4148771856494564E-5 2 0.0 0.0 0.0 0.0 3.4148771856494564E-5 3 0.0 0.0 0.0 0.0 3.4148771856494564E-5 4 0.0 0.0 0.0 0.0 3.4148771856494564E-5 5 0.0 0.0 0.0 0.0 3.4148771856494564E-5 6 0.0 0.0 0.0 0.0 3.4148771856494564E-5 7 0.0 0.0 0.0 0.0 3.4148771856494564E-5 8 0.0 0.0 0.0 0.0 3.4148771856494564E-5 9 0.0 0.0 0.0 0.0 3.4148771856494564E-5 10-11 0.0 0.0 0.0 0.0 3.4148771856494564E-5 12-13 0.0 0.0 0.0 0.0 4.6100842006267665E-4 14-15 0.0 0.0 0.0 0.0 5.293059637756657E-4 16-17 0.0 0.0 0.0 0.0 5.46380349703913E-4 18-19 0.0 0.0 0.0 0.0 5.97603507488655E-4 20-21 0.0 0.0 0.0 0.0 6.317522793451494E-4 22-23 0.0 0.0 0.0 6.829754371298913E-5 6.829754371298914E-4 24-25 0.0 0.0 0.0 2.7319017485195657E-4 6.829754371298914E-4 26-27 0.0 0.0 0.0 7.512729808428804E-4 7.000498230581387E-4 28-29 0.0 0.0 0.0 0.0033295052560082207 7.171242089863859E-4 30-31 0.0 0.0 0.0 0.01301068207732443 7.171242089863859E-4 32-33 0.0 0.0 0.0 0.032697449052593544 8.537192964123641E-4 34-35 0.0 0.0 0.0 0.06329474863601267 9.049424541971061E-4 36-37 0.0 0.0 0.0 0.11402274922883535 9.220168401253533E-4 38-39 0.0 0.0 0.0 0.1873060136328727 9.220168401253533E-4 40-41 0.0 0.0 0.0 0.2704753474893652 9.220168401253533E-4 42-43 0.0 0.0 0.0 0.35400324345035095 9.220168401253533E-4 44-45 0.0 0.0 0.0 0.4615035772545958 9.390912260536005E-4 46-47 0.0 0.0 0.0 0.5887248268059664 9.561656119818478E-4 48-49 0.0 0.0 0.0 0.7213757310825195 0.001024463155694837 50-51 0.0 0.0 0.0 0.8689325742744325 0.001092760699407826 52-53 0.0 0.0 0.0 1.0084644560800693 0.0011952070149773098 54-55 0.0 0.0 0.0 1.1624924915387882 0.001263504558690299 56-57 0.0 0.0 0.0 1.3478690995617688 0.0012805789446185462 58-59 0.0 0.0 0.0 1.5455904886108724 0.0012976533305467935 60-61 0.0 0.0 0.0 1.755400542897175 0.0013659508742597825 62-63 0.0 0.0 0.0 1.9613347115777655 0.0014000996461162772 64-65 0.0 0.0 0.0 2.182055298472218 0.0014683971898292662 66-67 0.0 0.0 0.0 2.43863209581599 0.0015025459616857609 68-69 0.0 0.0 0.0 2.7212814804721956 0.0015025459616857609 70-71 0.0 0.0 0.0 3.0237713015770247 0.00157084350539875 72-73 0.0 0.0 0.0 3.334798315645977 0.0017586617506094703 74-75 0.0 0.0 0.0 3.645278949365226 0.0018781824521072012 76-77 0.0 0.0 0.0 3.989259528275695 0.0018781824521072012 78-79 0.0 0.0 0.0 4.379494618665786 0.0018781824521072012 80-81 0.0 0.0 0.0 4.789279880943722 0.0019123312239636959 82-83 0.0 0.0 0.0 5.21422719792594 0.0020318519254614266 84-85 0.0 0.0 0.0 5.636066976669218 0.0020660006973179214 86-87 0.0 0.0 0.0 6.076569059232069 0.002185521398815652 88-89 0.0 0.0 0.0 6.54483409331425 0.002287967714385136 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4600 0.0 43.38424 1 GTATCAA 7815 0.0 38.365505 1 ATCAACG 9595 0.0 30.939436 3 TCAACGC 9675 0.0 30.732174 4 TATCAAC 9770 0.0 30.486647 2 CAACGCA 9895 0.0 30.048893 5 AACGCAG 10285 0.0 28.909458 6 TAAGGTG 1780 0.0 26.4171 5 ACGCAGA 11670 0.0 25.397074 7 CGCAGAG 11865 0.0 24.979675 8 TACATGG 8395 0.0 23.766504 2 GTACATG 8895 0.0 23.557714 1 GTAAGGT 1935 0.0 22.582748 4 ACATGGG 8595 0.0 22.215574 3 GCAGAGT 13235 0.0 21.783854 9 AGAGTAC 11070 0.0 20.938335 10-11 GGTAAGG 1980 0.0 20.630543 3 TGGTATC 2600 0.0 20.463568 2 AGGTAAG 1980 0.0 20.153515 2 GAGTACT 7605 0.0 19.954535 12-13 >>END_MODULE