FastQCFastQC Report
Thu 26 May 2016
SRR938467_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938467_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2234393
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47420.21222766093520704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39040.1747230679652147No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30990.13869538617423166No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA29990.13421989775299153No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA27170.12159902040509436No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26580.11895848223656268No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25750.11524382684693338No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC22860.1023096653095494No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32350.054.2425771
GTATCAA56500.040.1474721
ATCAACG69850.031.6566583
CGATACG300.003959607331.65449358-59
TATCAAC70850.031.6125562
TCAACGC70600.031.3203644
CAACGCA73400.029.9312215
AACGCAG76150.028.8920366
TAAGGTG14100.024.9569265
CGCAGAG87350.024.8644438
ACGCAGA88000.024.7893547
TACATGG60700.023.8157022
GTACATG63600.023.5527531
GTAAGGT13750.022.8254724
AGGTAAG13200.022.6957842
GTGGTAT16550.022.1248841
ACATGGG62500.021.7602833
TGGTATC15750.021.7385432
GCAGAGT98250.021.4771129
GTACGGG2005.33204E-721.399361
Produced by FastQC (version 0.11.5)