##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938467_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2234393 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.840770177851436 31.0 31.0 34.0 27.0 34.0 2 30.894123817967564 31.0 31.0 34.0 27.0 34.0 3 30.931703151594192 31.0 31.0 34.0 27.0 34.0 4 34.57856697546045 37.0 35.0 37.0 30.0 37.0 5 34.54457116541271 37.0 35.0 37.0 30.0 37.0 6 34.544581906584924 37.0 35.0 37.0 30.0 37.0 7 34.43704487079936 37.0 35.0 37.0 30.0 37.0 8 34.395760727857635 37.0 35.0 37.0 30.0 37.0 9 35.854373872456634 39.0 35.0 39.0 30.0 39.0 10-11 35.67444581145752 38.0 35.0 39.0 30.0 39.0 12-13 35.51991860876757 38.0 35.0 39.0 30.0 39.0 14-15 36.438509474385214 39.0 36.0 41.0 29.5 41.0 16-17 36.24280554047564 39.0 35.5 40.0 29.0 41.0 18-19 36.38547404149583 39.0 36.0 41.0 29.0 41.0 20-21 36.34625936440008 39.0 36.0 41.0 29.0 41.0 22-23 36.25109302615968 39.0 35.5 41.0 27.5 41.0 24-25 36.10455591294817 39.0 35.0 41.0 27.0 41.0 26-27 35.879190455752415 39.0 35.0 40.0 27.0 41.0 28-29 35.655700004430734 38.5 34.0 40.0 27.0 41.0 30-31 35.38660499741988 38.0 34.0 40.0 26.5 41.0 32-33 35.097443466749134 38.0 34.0 40.0 25.0 41.0 34-35 34.8296969243996 38.0 34.0 40.0 25.0 41.0 36-37 34.5330561812537 38.0 33.0 40.0 24.0 41.0 38-39 34.37384582747977 38.0 33.0 40.0 24.0 41.0 40-41 34.25177576191834 38.0 33.0 40.0 23.5 41.0 42-43 33.93563509194667 38.0 33.0 40.0 22.0 41.0 44-45 33.586134802606345 38.0 32.5 40.0 20.0 41.0 46-47 33.20566749000736 37.5 31.5 40.0 17.5 41.0 48-49 32.83441341787233 37.0 31.0 40.0 15.0 41.0 50-51 32.1450017969086 36.5 30.5 39.5 14.0 40.5 52-53 32.17291206157556 36.5 31.0 39.0 12.5 40.0 54-55 32.253471972029985 37.0 31.0 39.5 9.0 41.0 56-57 31.91929217465325 36.0 30.5 39.0 8.0 41.0 58-59 31.52422223843344 36.0 30.0 39.0 6.5 41.0 60-61 31.022184101006403 35.5 29.0 39.0 2.0 40.0 62-63 30.437979352781717 35.0 28.0 38.5 2.0 40.0 64-65 29.901751840432723 34.5 28.0 38.0 2.0 40.0 66-67 29.34780922604036 34.0 27.0 38.0 2.0 40.0 68-69 28.73290755028323 34.0 26.0 37.0 2.0 39.5 70-71 28.06818496119528 34.0 25.5 36.5 2.0 39.0 72-73 27.417154233834424 33.0 24.5 36.0 2.0 39.0 74-75 26.72168481551813 32.5 22.5 35.5 2.0 38.5 76-77 25.95135994428912 32.0 20.5 35.0 2.0 37.5 78-79 25.14340874680506 31.0 18.0 35.0 2.0 37.0 80-81 24.43326845366952 31.0 12.0 34.5 2.0 36.0 82-83 23.677672638609234 30.0 7.0 34.0 2.0 36.0 84-85 22.954781007638317 30.0 2.0 34.0 2.0 35.0 86-87 22.17259855361165 29.0 2.0 34.0 2.0 35.0 88-89 21.32171063908632 29.0 2.0 34.0 2.0 35.0 90-91 20.394770749818854 27.0 2.0 33.5 2.0 35.0 92-93 19.446706107654293 26.0 2.0 33.0 2.0 35.0 94-95 18.433025658422668 24.5 2.0 32.5 2.0 34.5 96-97 17.193315813287995 20.5 2.0 32.0 2.0 34.0 98-99 15.698934117677597 2.0 2.0 32.0 2.0 34.0 100-101 12.399265035291464 2.0 2.0 27.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5432645534248337 1101 2 0.5514698096477595 1101 3 0.5504145168215508 1101 4 0.4528661648578165 1101 5 0.4961105569341058 1101 6 0.7278605478280085 1101 7 0.6429544401886105 1101 8 0.526410589292233 1101 9 0.7652904475258495 1101 10-11 0.9105300319600289 1101 12-13 0.818734497578042 1101 14-15 0.9825886096560126 1101 16-17 0.8924479447085432 1101 18-19 1.0900871877225953 1101 20-21 1.1852728930965526 1101 22-23 1.1034903609159556 1101 24-25 1.1097150032988097 1101 26-27 1.0755448392982316 1101 28-29 1.1688152348533762 1101 30-31 1.1169667874346985 1101 32-33 1.205234123235165 1101 34-35 1.2192795943701285 1101 36-37 1.0426269139179212 1101 38-39 0.9857553627763025 1101 40-41 1.1898850226495554 1101 42-43 1.3100579350534787 1101 44-45 1.3267684530563955 1101 46-47 1.2293293938536394 1101 48-49 0.8897189689253935 1101 50-51 1.1563565250344396 1101 52-53 1.1463224946194472 1101 54-55 1.168627873892767 1101 56-57 1.1167857581577927 1101 58-59 1.2070489210689317 1101 60-61 1.3694910343676021 1101 62-63 1.2969613281806716 1101 64-65 1.0994057305956844 1101 66-67 1.1466731300651425 1101 68-69 1.2019576059653012 1101 70-71 1.0511387852570344 1101 72-73 1.1677327596213765 1101 74-75 1.2166699694170227 1101 76-77 1.1426640914590784 1101 78-79 1.4818203145813662 1101 80-81 1.3205153806283114 1101 82-83 1.4081033271146346 1101 84-85 1.3520834249544293 1101 86-87 1.460509575827377 1101 88-89 1.3990067568776254 1101 90-91 0.857381411445548 1101 92-93 0.9576126663748923 1101 94-95 0.8898352417774227 1101 96-97 1.0732668007715347 1101 98-99 0.9360337691808045 1101 100-101 1.1237834912156988 1102 1 0.5527047724393555 1102 2 0.591609012724355 1102 3 0.5052608177481552 1102 4 0.39746556011352396 1102 5 0.49493210864191184 1102 6 0.47823794905175276 1102 7 0.46336243956059064 1102 8 0.5398711577446491 1102 9 0.7400117842074394 1102 10-11 0.7840123060584432 1102 12-13 0.763839876662118 1102 14-15 0.8663333546879812 1102 16-17 0.8473104876483717 1102 18-19 0.8539745601889521 1102 20-21 0.8295585919136812 1102 22-23 0.904732897485296 1102 24-25 0.8908252261121632 1102 26-27 0.8542231590078302 1102 28-29 0.6991083941685758 1102 30-31 0.8601321708319247 1102 32-33 0.9233335685704205 1102 34-35 0.7325616134689312 1102 36-37 0.97428176301608 1102 38-39 0.7656837535446996 1102 40-41 0.9346991959716817 1102 42-43 0.9971279164744189 1102 44-45 1.0625234179240195 1102 46-47 1.0614078436798025 1102 48-49 1.1230235328408753 1102 50-51 1.0076197250539423 1102 52-53 0.8281067158645428 1102 54-55 0.9138303892392088 1102 56-57 0.9857300814388203 1102 58-59 0.9792412635011019 1102 60-61 0.9462239443859453 1102 62-63 1.066727068721729 1102 64-65 1.0151814175651666 1102 66-67 0.7585486810528828 1102 68-69 0.9914628065677 1102 70-71 1.318595565711032 1102 72-73 1.5085185076157153 1102 74-75 1.3071113641728367 1102 76-77 1.3341173861965743 1102 78-79 1.4542170131214789 1102 80-81 1.4093269344746453 1102 82-83 1.530695787030698 1102 84-85 1.863696238820058 1102 86-87 1.8934994281784547 1102 88-89 1.7418715953553168 1102 90-91 1.4351896780888715 1102 92-93 1.7555197250636034 1102 94-95 1.9718235709885938 1102 96-97 2.1531636324059242 1102 98-99 2.275857290683879 1102 100-101 1.7722268070407434 1103 1 0.49521767096268476 1103 2 0.40581176889565285 1103 3 0.3793263525766335 1103 4 0.3535570021463599 1103 5 0.24720073568695256 1103 6 0.4879013937129386 1103 7 0.5337759252630221 1103 8 0.4933518194966524 1103 9 0.580656888255028 1103 10-11 0.6938664478378271 1103 12-13 0.6768115455598007 1103 14-15 0.963381535607212 1103 16-17 0.930854277904686 1103 18-19 0.8871741397894652 1103 20-21 0.6066163441223011 1103 22-23 0.628259473994099 1103 24-25 0.8400440280164787 1103 26-27 0.82989491736042 1103 28-29 1.0160169791393798 1103 30-31 1.1012894438759986 1103 32-33 0.9472666141221282 1103 34-35 1.0568835542675288 1103 36-37 1.097558941641715 1103 38-39 1.1260067942057503 1103 40-41 1.2332670601853906 1103 42-43 1.1940294419227726 1103 44-45 1.2585749717261976 1103 46-47 1.1063547558138183 1103 48-49 0.9287400743692587 1103 50-51 1.2365519583533668 1103 52-53 1.3774942262105796 1103 54-55 1.112277658635648 1103 56-57 1.1762810370889873 1103 58-59 1.2193241775279517 1103 60-61 1.2341456244065547 1103 62-63 1.2097459425500148 1103 64-65 1.3562203246636528 1103 66-67 1.3047965898783147 1103 68-69 1.2859345832656182 1103 70-71 1.3718237005869192 1103 72-73 1.6040406615287317 1103 74-75 1.4939765375811973 1103 76-77 1.503478308675131 1103 78-79 1.3581607872613368 1103 80-81 1.5413093572495988 1103 82-83 1.4527693963754977 1103 84-85 1.2402630391761633 1103 86-87 1.45266493433164 1103 88-89 1.4113165291513994 1103 90-91 1.3641821123553761 1103 92-93 1.4879160321661153 1103 94-95 1.5409463470997231 1103 96-97 1.7742819130543026 1103 98-99 1.7550697403038793 1103 100-101 1.4526369322904742 1104 1 0.5392030514093982 1104 2 0.4583171378465636 1104 3 0.4981059788176161 1104 4 0.40524626630601546 1104 5 0.4633581553891517 1104 6 0.5402145596780983 1104 7 0.4236975767777835 1104 8 0.7541555068836487 1104 9 0.6652286272615626 1104 10-11 0.7241726750933069 1104 12-13 0.8873220172877723 1104 14-15 0.98576226971349 1104 16-17 1.110216952206116 1104 18-19 1.0067083294871466 1104 20-21 0.9109221760580652 1104 22-23 0.9153564692005531 1104 24-25 1.019285741763781 1104 26-27 1.1016290127149233 1104 28-29 1.0660961643442377 1104 30-31 1.1770131760335616 1104 32-33 1.1545933070545118 1104 34-35 0.9802846012584254 1104 36-37 1.1144655285653684 1104 38-39 1.2320604000428972 1104 40-41 1.2925237863804142 1104 42-43 1.3372085399390201 1104 44-45 1.2195644351221375 1104 46-47 0.9927918945237408 1104 48-49 0.9185181496473405 1104 50-51 1.0588790938983053 1104 52-53 1.1491595044077272 1104 54-55 1.2604052256368732 1104 56-57 1.1836884310226488 1104 58-59 1.3352892729806811 1104 60-61 1.5120356721723276 1104 62-63 1.472235498712699 1104 64-65 1.3875915504821563 1104 66-67 1.4867391496825455 1104 68-69 1.56645241570099 1104 70-71 1.7213878788009218 1104 72-73 1.7697020520112297 1104 74-75 1.6226355185537074 1104 76-77 1.40812713502428 1104 78-79 1.5117340044725402 1104 80-81 1.7340331706538485 1104 82-83 1.6297219543729007 1104 84-85 1.7988794929643817 1104 86-87 2.203308238331278 1104 88-89 1.994018078537863 1104 90-91 1.855646359568432 1104 92-93 2.209060422082313 1104 94-95 1.9286879802066537 1104 96-97 2.016437363433795 1104 98-99 2.17776078825276 1104 100-101 1.7083648237934046 1105 1 0.48205141062519274 1105 2 0.3856207941836658 1105 3 0.48231884743776376 1105 4 0.39378478550120377 1105 5 0.48145069983438304 1105 6 0.6062124984502617 1105 7 0.5895362388535332 1105 8 0.55342997810844 1105 9 0.7169098162152565 1105 10-11 0.718634003648333 1105 12-13 0.7165905741940577 1105 14-15 0.9128706364246568 1105 16-17 0.7374690778473223 1105 18-19 0.6921719886350175 1105 20-21 0.7323226398343365 1105 22-23 0.7676244053961838 1105 24-25 0.784917109903752 1105 26-27 0.6565250144013817 1105 28-29 0.6943423053978037 1105 30-31 0.8050886216569566 1105 32-33 1.022074802850078 1105 34-35 1.087524956820232 1105 36-37 0.9013372054210507 1105 38-39 1.1811928690659244 1105 40-41 1.278629529710706 1105 42-43 1.2973864379550903 1105 44-45 1.2317745749577043 1105 46-47 1.3855632504837416 1105 48-49 1.3412054168808538 1105 50-51 1.354665873932504 1105 52-53 1.443816708164782 1105 54-55 1.4796141708972215 1105 56-57 1.426273866574146 1105 58-59 1.4001257215788172 1105 60-61 1.361437798984685 1105 62-63 1.3626308796885063 1105 64-65 1.5385527736334907 1105 66-67 1.5756137895532483 1105 68-69 1.7706834957375328 1105 70-71 1.9113774555465248 1105 72-73 1.8131274964634834 1105 74-75 1.9399126482939444 1105 76-77 1.9972471312765911 1105 78-79 1.8939810941593755 1105 80-81 1.8405289662175228 1105 82-83 2.119740809922991 1105 84-85 2.0258036959953216 1105 86-87 2.006205376035357 1105 88-89 1.9270647723752603 1105 90-91 1.5518524120828978 1105 92-93 1.6998370130925444 1105 94-95 1.9709651099468921 1105 96-97 2.56159786984 1105 98-99 2.678913494785446 1105 100-101 2.0647461392315325 1106 1 0.4028119658594349 1106 2 0.23466166559295942 1106 3 0.24987620236354147 1106 4 0.20299916761032222 1106 5 0.30923265472159756 1106 6 0.044394491895410226 1106 7 0.3597367401066691 1106 8 0.3322664924712697 1106 9 0.28922232826461425 1106 10-11 0.1630792513830599 1106 12-13 0.26925348146222916 1106 14-15 0.5374585485125678 1106 16-17 0.4509132652388743 1106 18-19 0.3603347031135158 1106 20-21 0.5042582480857334 1106 22-23 0.4117105823770899 1106 24-25 0.2858579234761507 1106 26-27 0.2290091092539015 1106 28-29 0.42552651592411905 1106 30-31 0.0653826258983301 1106 32-33 0.3896451710783424 1106 34-35 0.057976566613945124 1106 36-37 0.02349500376306679 1106 38-39 0.32913821319547765 1106 40-41 0.3907121400827904 1106 42-43 -0.18396019786522544 1106 44-45 0.19345714677185555 1106 46-47 0.21292540587220543 1106 48-49 0.5852361668326509 1106 50-51 0.1673176957948499 1106 52-53 0.3132209880953809 1106 54-55 0.3582313870144418 1106 56-57 -0.4511080497551383 1106 58-59 0.027112901065990513 1106 60-61 0.5795725589615515 1106 62-63 0.39616278637830504 1106 64-65 0.6552420930859668 1106 66-67 0.2452041115119954 1106 68-69 0.2894375925179453 1106 70-71 0.05844994446111684 1106 72-73 0.30756792445654213 1106 74-75 0.665625488085734 1106 76-77 0.6396145883808906 1106 78-79 0.7897580365152628 1106 80-81 1.1137360223193191 1106 82-83 0.782446612891956 1106 84-85 0.8732829711061783 1106 86-87 0.6670705196636568 1106 88-89 0.813408034376419 1106 90-91 1.0075850128154933 1106 92-93 0.9553498336053643 1106 94-95 1.067721905782637 1106 96-97 1.0200010894274314 1106 98-99 1.1066197797285735 1106 100-101 0.8596386589924734 1107 1 0.09952719295634083 1107 2 -0.056898512990120054 1107 3 0.11963414586194432 1107 4 0.09886603187413101 1107 5 0.009787604436851893 1107 6 0.11491523493523914 1107 7 -0.07382923590205337 1107 8 -0.05416454892160516 1107 9 0.0807897769312973 1107 10-11 0.24451956679283882 1107 12-13 0.21663019296107677 1107 14-15 0.3328345889267723 1107 16-17 0.40971920060970035 1107 18-19 0.4540910993889966 1107 20-21 0.43834692446448287 1107 22-23 0.3850466186902324 1107 24-25 0.4361514298001907 1107 26-27 0.3555239430206427 1107 28-29 0.40942812745932144 1107 30-31 0.36987479191721917 1107 32-33 0.5565224837571847 1107 34-35 0.5669449603915027 1107 36-37 0.5946873906806402 1107 38-39 0.7528721467767383 1107 40-41 0.5661139054082227 1107 42-43 0.7849148965060451 1107 44-45 0.6075105912069816 1107 46-47 0.5975297921559886 1107 48-49 0.7382574458081308 1107 50-51 0.6335407009491547 1107 52-53 0.8448099673942835 1107 54-55 0.9929199384968825 1107 56-57 1.0026030483071047 1107 58-59 0.8425027359978117 1107 60-61 0.8007072512412812 1107 62-63 1.0554437005447141 1107 64-65 1.0767190385323815 1107 66-67 1.1754184857721697 1107 68-69 1.291216619258801 1107 70-71 1.47927286200159 1107 72-73 1.5238608342609012 1107 74-75 1.36482838866268 1107 76-77 1.2636928256652347 1107 78-79 1.4250991246044435 1107 80-81 1.4155423584767988 1107 82-83 1.2786343861026275 1107 84-85 1.4139548946025293 1107 86-87 1.4669331337247407 1107 88-89 1.2430858205427526 1107 90-91 1.2612459659420985 1107 92-93 1.4655918901069676 1107 94-95 1.5637985772028173 1107 96-97 1.6581499210877126 1107 98-99 1.6499923528721414 1107 100-101 1.3221102077999003 1108 1 0.2123876679351362 1108 2 0.0911215251778188 1108 3 0.13747235620969533 1108 4 0.2037582869631933 1108 5 0.13597372615016923 1108 6 0.33618325868418 1108 7 0.2362421964558763 1108 8 0.3740485819096051 1108 9 0.32044600042578253 1108 10-11 0.5647094787945335 1108 12-13 0.40744172550984104 1108 14-15 0.5379574419802111 1108 16-17 0.5860322174672064 1108 18-19 0.5981021786934946 1108 20-21 0.5458483160039265 1108 22-23 0.42152151796927484 1108 24-25 0.5706056150207175 1108 26-27 0.6068608148399761 1108 28-29 0.42813936314635015 1108 30-31 0.4897194696101792 1108 32-33 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-2.7780016957562843 2315 92-93 -2.758213948316623 2315 94-95 -2.936434679866487 2315 96-97 -3.0599912860873335 2315 98-99 -3.1395074693837497 2315 100-101 -2.7830728207339313 2316 1 -0.939226077862994 2316 2 -0.9684499407075258 2316 3 -0.8622465726537563 2316 4 -0.9061997936900283 2316 5 -0.9677606871777158 2316 6 -1.014673014596461 2316 7 -1.0040221665631108 2316 8 -1.120781767527113 2316 9 -1.2376952676309045 2316 10-11 -1.3272161849875985 2316 12-13 -1.431377285971486 2316 14-15 -1.583787172242559 2316 16-17 -1.5913696276654363 2316 18-19 -1.4122555765985894 2316 20-21 -1.37528017751157 2316 22-23 -1.4469418036669524 2316 24-25 -1.438328862754794 2316 26-27 -1.5952426406315396 2316 28-29 -1.6141382428657423 2316 30-31 -1.420711108658196 2316 32-33 -1.5208483011076908 2316 34-35 -1.5549975647330356 2316 36-37 -1.535489658345064 2316 38-39 -1.7303409993336132 2316 40-41 -1.8183449434714376 2316 42-43 -1.7464355557044442 2316 44-45 -1.5935257721897997 2316 46-47 -1.7715764046639393 2316 48-49 -1.8102020008867932 2316 50-51 -1.7554699009093433 2316 52-53 -1.974092230166324 2316 54-55 -2.2574906260501706 2316 56-57 -2.1640547759643347 2316 58-59 -2.1595806016700294 2316 60-61 -1.9570079986656133 2316 62-63 -2.203503589763951 2316 64-65 -2.3228308315364252 2316 66-67 -2.3979990549045844 2316 68-69 -2.4257584216069326 2316 70-71 -2.830644248345255 2316 72-73 -2.5966785241071584 2316 74-75 -2.6271727112619345 2316 76-77 -2.8153914766905856 2316 78-79 -3.1127778821858207 2316 80-81 -3.255271911692706 2316 82-83 -3.4695761558356395 2316 84-85 -3.5246908457556216 2316 86-87 -3.517361385307904 2316 88-89 -3.776331212476485 2316 90-91 -3.6611824748072657 2316 92-93 -3.6420613243290934 2316 94-95 -3.90227315230727 2316 96-97 -4.068571866643481 2316 98-99 -3.9292041265368685 2316 100-101 -3.2944247591919584 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11080.0 3 4054.0 4 4298.0 5 4836.0 6 5511.0 7 6912.0 8 8385.0 9 10259.0 10 12763.0 11 15222.0 12 18113.0 13 20527.0 14 23297.0 15 25691.0 16 29077.0 17 31802.0 18 31656.0 19 31350.0 20 33165.0 21 35885.0 22 39529.0 23 43391.0 24 47971.0 25 53576.0 26 58913.0 27 66238.0 28 73811.0 29 83543.0 30 94800.0 31 108847.0 32 126197.0 33 147710.0 34 175407.0 35 207698.0 36 233284.0 37 212799.0 38 92378.0 39 4418.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.23984769812827 18.279268138213904 14.345907144554742 24.13497701910308 2 15.489343247258528 19.4785691116356 40.23248978948142 24.79959785162445 3 19.325922643437803 22.996915971307736 29.653453390751316 28.023707994503145 4 12.952253014796058 16.007981205721265 36.603273764049 34.43649201543368 5 14.02649924513281 36.91582961876517 34.33556446494081 14.722106671161207 6 33.30678254636681 35.528940059134484 16.964922497984052 14.199354896514649 7 29.253952777083875 32.118065348522165 20.18506132747412 18.44292054691984 8 26.679932581100772 35.00656953749113 19.429983503410117 18.883514377997983 9 26.339576693048738 16.595727536439494 19.717168642337857 37.34752712817391 10-11 25.22848882469616 26.111009488378922 27.329123987917814 21.3313776990071 12-13 26.08110598211641 23.275602849678116 27.454829234478694 23.18846193372678 14-15 22.608492443346925 24.969300187534106 25.594533262122006 26.827674106996962 16-17 22.60547959801776 28.018302933653764 26.580845269429016 22.79537219889946 18-19 22.68795557160392 26.642016559910974 28.513541254155705 22.156486614329403 20-21 22.95408511242383 26.016682318095246 28.79050059045518 22.23873197902574 22-23 22.67937988903725 25.87518313778117 28.687037818441485 22.75839915474009 24-25 22.570797583592935 26.05317044840373 28.813943730904928 22.56208823709841 26-27 22.16286426440466 26.475843483073913 28.45012812004324 22.911164132478184 28-29 22.325882774662713 26.948850353772606 28.268716273719086 22.45655059784559 30-31 22.422676949535123 26.32521810788055 28.30697676501675 22.945128177567582 32-33 22.08784196774605 26.318401945177694 28.34751692067266 23.246239166403594 34-35 22.913527601049644 26.516385614632128 28.078534691447345 22.491552092870887 36-37 23.005546588499914 26.060905674858816 28.082579305826748 22.85096843081452 38-39 22.40130620226705 26.49079914453959 28.050554898683817 23.057339754509545 40-41 22.9659812655639 26.25357393261083 28.139083622635695 22.64136117918957 42-43 22.71069863937005 26.207286823336585 27.975637912111406 23.106376625181962 44-45 22.595428354798983 26.604217070903896 28.1296653408548 22.670689233442324 46-47 23.034273031665723 26.80173501361956 27.454211499153914 22.70978045556081 48-49 22.748940108996898 26.82388084819926 27.411296790965146 23.015882251838697 50-51 22.92300610085102 27.048807031968764 27.394792714069688 22.633394153110526 52-53 23.274636784190314 26.654699532198585 27.405155685019235 22.665507998591874 54-55 22.68018418741013 26.88780345882919 27.64715690059363 22.78485545316705 56-57 22.72428287289949 26.980706293736027 27.473148228760014 22.821862604604465 58-59 22.699864209774525 26.569605863666794 27.43741440370141 23.293115522857274 60-61 22.353881525876094 26.83824878975109 27.72224306328391 23.085626621088906 62-63 22.51304471402339 26.87988792120467 27.84862648644838 22.75844087832356 64-65 22.245879777199246 26.89299293011102 27.653826761200406 23.207300531489327 66-67 22.151211479525497 26.90830460900675 27.610892461581916 23.329591449885843 68-69 22.773581029274393 26.442290692110642 27.76926840207203 23.014859876542936 70-71 22.56838926186515 26.57383497397125 27.346079391713417 23.511696372450178 72-73 22.517749308449606 26.687062870537382 27.4555690903499 23.339618730663116 74-75 22.489054671465546 26.98280062971314 27.368458516762768 23.159686182058547 76-77 22.665492015145126 26.5990301962438 27.424554156180303 23.310923632430768 78-79 22.723831506007205 26.752475243093343 27.350924270529752 23.1727689803697 80-81 22.85949004301821 26.7391178347092 27.11713560458786 23.284256517684728 82-83 22.696140905240757 26.824250005874404 27.276568271256686 23.20304081762815 84-85 22.657373298753985 26.880431136363075 27.23669779300415 23.22549777187879 86-87 22.845461568830018 26.859542419356714 26.904929864312127 23.39006614750114 88-89 22.73337704618571 26.859607645436085 26.81532767513814 23.591687633240067 90-91 22.623666283646095 26.825028099741154 26.915712864857316 23.635592751755436 92-93 22.794633143962287 26.408866862753 27.1535696218399 23.642930371444816 94-95 22.587277866398008 26.221610276215262 27.319060812134445 23.872051045252288 96-97 22.829771891455515 25.87706975297849 27.304810605051472 23.98834775051452 98-99 22.612420821777008 26.066683066184588 26.91644371007234 24.40445240196606 100-101 23.166197049361102 25.46828481964859 27.28006256189341 24.085455569096897 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 121.0 1 108.0 2 117.5 3 140.5 4 121.0 5 131.5 6 242.0 7 520.0 8 846.5 9 1013.0 10 940.0 11 853.0 12 905.5 13 1002.0 14 1180.0 15 1349.0 16 1503.0 17 1679.0 18 1936.0 19 2323.5 20 2795.5 21 3423.5 22 4193.5 23 5266.5 24 6821.0 25 8839.0 26 11556.0 27 14781.5 28 17758.5 29 20692.5 30 23901.5 31 28827.5 32 36120.0 33 39875.5 34 40840.5 35 43660.0 36 47638.5 37 51969.0 38 55910.0 39 59177.0 40 62912.5 41 67955.5 42 70706.5 43 72651.0 44 75535.0 45 79466.5 46 81951.0 47 84176.5 48 86262.0 49 85496.5 50 84114.5 51 82281.5 52 80326.5 53 79142.5 54 79036.0 55 75074.5 56 68812.5 57 63285.5 58 58661.0 59 51887.0 60 42150.0 61 35278.0 62 30161.0 63 23521.5 64 17221.5 65 13101.0 66 10072.0 67 7862.5 68 6114.0 69 4811.5 70 3760.0 71 2614.5 72 1886.0 73 1443.5 74 1050.5 75 775.0 76 594.0 77 413.5 78 277.5 79 187.5 80 115.5 81 79.5 82 50.0 83 30.5 84 20.0 85 13.0 86 7.0 87 7.5 88 7.0 89 4.0 90 1.5 91 1.5 92 2.0 93 1.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006534213095010591 2 0.1065613793097275 3 0.11510956219429617 4 0.11860044316286347 5 0.15829802545926344 6 0.09814746107779607 7 0.02895641008542365 8 0.026763420759015985 9 0.024749450969457926 10-11 0.05314642500222655 12-13 0.05498137525493501 14-15 0.02980675288545927 16-17 0.0341032217698498 18-19 0.038780107170045736 20-21 0.0613141913709898 22-23 0.034036089443531196 24-25 0.05191566568638552 26-27 0.05956875088670614 28-29 0.05592122782339543 30-31 0.06950433518185924 32-33 0.05272125360220874 34-35 0.06460367536060128 36-37 0.054533826412810994 38-39 0.033208124085601774 40-41 0.03844444553845272 42-43 0.03394657967510639 44-45 0.007362178452940015 46-47 0.012956538979490178 48-49 0.008995731726692663 50-51 0.008794334747736858 52-53 0.009778942200409686 54-55 0.011434872916268535 56-57 0.014433450158499422 58-59 0.019356487421863564 60-61 0.024838960737882727 62-63 0.011211098495206528 64-65 0.008928599400374061 66-67 0.010025094063577893 68-69 0.011457250358374735 70-71 0.03173121290659253 72-73 0.028822145432786443 74-75 0.030321434053901884 76-77 0.024122882590484306 78-79 0.005258698894957154 80-81 0.004766395168620739 82-83 0.0056167379686563644 84-85 0.004990169589682746 86-87 0.013202690842658385 88-89 0.03927241089638215 90-91 0.03721368622261169 92-93 0.030567585917070095 94-95 0.04835765239149962 96-97 0.04495628119135712 98-99 0.026293494474785767 100-101 0.07715742038217985 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2234393.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.291008708696026 #Duplication Level Percentage of deduplicated Percentage of total 1 77.81420628898017 45.35868576451212 2 12.68581828005854 14.78938287679655 3 4.215352739891838 7.371514898137822 4 1.8446250419962043 4.30100217549118 5 1.007557264962689 2.936576465322502 6 0.5923482896688169 2.0717147586999722 7 0.36826489726646405 1.5026572633566548 8 0.2602597766576957 1.2136643926141615 9 0.17920552396446518 0.9401463682253166 >10 0.9368422068235703 9.718262577759306 >50 0.05664168742982046 2.266897747343832 >100 0.03339426606624691 3.5907904286917716 >500 0.0027804859775367196 1.1226106337981434 >1k 0.0027032502559380354 2.8160936492506665 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4742 0.21222766093520704 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3904 0.1747230679652147 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3099 0.13869538617423166 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2999 0.13421989775299153 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2717 0.12159902040509436 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2658 0.11895848223656268 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2575 0.11524382684693338 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2286 0.1023096653095494 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.475488421240131E-5 2 0.0 0.0 0.0 0.0 4.475488421240131E-5 3 0.0 0.0 0.0 0.0 4.475488421240131E-5 4 0.0 0.0 0.0 0.0 4.475488421240131E-5 5 0.0 0.0 0.0 4.475488421240131E-5 4.475488421240131E-5 6 0.0 0.0 0.0 4.475488421240131E-5 4.475488421240131E-5 7 0.0 0.0 0.0 4.475488421240131E-5 4.475488421240131E-5 8 0.0 0.0 0.0 4.475488421240131E-5 4.475488421240131E-5 9 0.0 0.0 0.0 4.475488421240131E-5 4.475488421240131E-5 10-11 0.0 0.0 0.0 4.475488421240131E-5 4.475488421240131E-5 12-13 2.2377442106200654E-5 0.0 0.0 4.475488421240131E-5 4.475488421240131E-5 14-15 1.7901953684960523E-4 0.0 0.0 6.713232631860196E-5 4.475488421240131E-5 16-17 1.7901953684960523E-4 0.0 0.0 8.950976842480262E-5 4.475488421240131E-5 18-19 2.2377442106200654E-4 0.0 0.0 1.1188721053100327E-4 4.475488421240131E-5 20-21 2.6852930527440785E-4 0.0 0.0 2.0139697895580589E-4 4.475488421240131E-5 22-23 2.6852930527440785E-4 0.0 0.0 3.1328418948680916E-4 4.475488421240131E-5 24-25 4.0279395791161177E-4 0.0 0.0 6.48945821079819E-4 4.475488421240131E-5 26-27 4.251714000178124E-4 0.0 0.0 0.001096494663203832 4.475488421240131E-5 28-29 4.6992628423021376E-4 4.475488421240131E-5 0.0 0.0031775967790804932 4.475488421240131E-5 30-31 4.923037263364144E-4 4.475488421240131E-5 0.0 0.012016686411029752 4.475488421240131E-5 32-33 4.923037263364144E-4 4.475488421240131E-5 0.0 0.029493468695972462 4.475488421240131E-5 34-35 5.14681168442615E-4 4.475488421240131E-5 0.0 0.05782331040242249 4.475488421240131E-5 36-37 5.370586105488157E-4 4.475488421240131E-5 0.0 0.10705368303606394 4.475488421240131E-5 38-39 5.370586105488157E-4 4.475488421240131E-5 0.0 0.17837059102852543 4.475488421240131E-5 40-41 5.594360526550164E-4 4.475488421240131E-5 0.0 0.254386761863289 4.475488421240131E-5 42-43 6.041909368674177E-4 4.475488421240131E-5 0.0 0.33360290691923933 4.475488421240131E-5 44-45 6.265683789736183E-4 4.475488421240131E-5 0.0 0.42993779518643316 4.475488421240131E-5 46-47 6.48945821079819E-4 4.475488421240131E-5 0.0 0.5438165980648884 4.475488421240131E-5 48-49 6.713232631860197E-4 4.475488421240131E-5 0.0 0.6717036797018251 4.475488421240131E-5 50-51 6.713232631860197E-4 4.475488421240131E-5 0.0 0.8064382586232592 4.475488421240131E-5 52-53 6.713232631860197E-4 4.475488421240131E-5 0.0 0.9378609761129757 4.475488421240131E-5 54-55 6.713232631860197E-4 4.475488421240131E-5 0.0 1.0814346446663592 4.475488421240131E-5 56-57 7.160781473984209E-4 4.475488421240131E-5 0.0 1.2532262677156614 4.475488421240131E-5 58-59 7.160781473984209E-4 4.475488421240131E-5 0.0 1.4392499439445077 4.475488421240131E-5 60-61 7.160781473984209E-4 6.713232631860196E-5 0.0 1.6422804761740661 4.475488421240131E-5 62-63 7.384555895046216E-4 8.950976842480262E-5 0.0 1.841350201150827 4.475488421240131E-5 64-65 8.055879158232235E-4 8.950976842480262E-5 0.0 2.051205853222777 4.475488421240131E-5 66-67 8.055879158232235E-4 8.950976842480262E-5 0.0 2.2966192608014797 4.475488421240131E-5 68-69 8.055879158232235E-4 8.950976842480262E-5 0.0 2.5696464319392334 4.475488421240131E-5 70-71 8.055879158232235E-4 8.950976842480262E-5 0.0 2.85072948223522 4.475488421240131E-5 72-73 8.503428000356248E-4 8.950976842480262E-5 0.0 3.14445578732121 4.475488421240131E-5 74-75 8.950976842480262E-4 8.950976842480262E-5 0.0 3.431826898849039 4.475488421240131E-5 76-77 8.950976842480262E-4 8.950976842480262E-5 0.0 3.7547333884415144 4.475488421240131E-5 78-79 8.950976842480262E-4 8.950976842480262E-5 0.0 4.114875941698708 4.475488421240131E-5 80-81 9.622300105666282E-4 8.950976842480262E-5 0.0 4.4910631209460465 4.475488421240131E-5 82-83 9.846074526728289E-4 8.950976842480262E-5 0.0 4.879311741488628 4.475488421240131E-5 84-85 9.846074526728289E-4 8.950976842480262E-5 0.0 5.279286141694858 4.475488421240131E-5 86-87 9.846074526728289E-4 8.950976842480262E-5 0.0 5.695954113712315 6.713232631860196E-5 88-89 9.846074526728289E-4 8.950976842480262E-5 0.0 6.138109992288733 8.950976842480262E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3235 0.0 54.242577 1 GTATCAA 5650 0.0 40.147472 1 ATCAACG 6985 0.0 31.656658 3 CGATACG 30 0.0039596073 31.654493 58-59 TATCAAC 7085 0.0 31.612556 2 TCAACGC 7060 0.0 31.320364 4 CAACGCA 7340 0.0 29.931221 5 AACGCAG 7615 0.0 28.892036 6 TAAGGTG 1410 0.0 24.956926 5 CGCAGAG 8735 0.0 24.864443 8 ACGCAGA 8800 0.0 24.789354 7 TACATGG 6070 0.0 23.815702 2 GTACATG 6360 0.0 23.552753 1 GTAAGGT 1375 0.0 22.825472 4 AGGTAAG 1320 0.0 22.695784 2 GTGGTAT 1655 0.0 22.124884 1 ACATGGG 6250 0.0 21.760283 3 TGGTATC 1575 0.0 21.738543 2 GCAGAGT 9825 0.0 21.477112 9 GTACGGG 200 5.33204E-7 21.39936 1 >>END_MODULE