FastQCFastQC Report
Thu 26 May 2016
SRR938467_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938467_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2234393
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47030.21048222045092335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42000.1879705136920855No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA30630.1370842103425852No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA30450.13627862242676198No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28150.1259849990579097No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27620.12361299019465243No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26640.11922701154183708No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC26270.11757108082597824No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG24300.10875436863613519No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31150.054.47491
GTATCAA52850.039.5725441
TCAACGC69200.029.9311584
ATCAACG69000.029.811373
TATCAAC70150.029.4745982
CAACGCA71850.028.7611085
AACGCAG75400.027.3928666
TGGTATC14900.026.4775712
GTAAGGT13850.025.0389564
TACATGG60600.025.0209832
TAAGGTG15200.024.3777795
GTGGTAT16800.024.048941
ACGCAGA86050.023.9474037
GTACATG64450.023.8950731
CGCAGAG86100.023.7680578
ACATGGG62700.023.5632933
GGTAAGG15600.021.9255773
GCGTTAC1100.002866973621.6054651
AGGTAAG16100.020.3708672
AAGGTAA15450.019.997291
Produced by FastQC (version 0.11.5)