FastQCFastQC Report
Thu 26 May 2016
SRR938466_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938466_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259215
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47850.2117992311488725No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39220.17360012216632767No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA30640.13562232899480572No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30220.13376327618221373No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA27520.12181222238697954No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA25720.11384485319015675No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25440.11260548464842876No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC22970.10167248358389971No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31150.049.659341
GTATCAA56950.041.955181
TCAACGC70700.032.9365654
ATCAACG70600.032.848593
TATCAAC72450.032.0754052
CAACGCA72550.032.0290645
AACGCAG76550.030.4748636
ACGCAGA85850.026.3964257
CGCAGAG86450.026.1582668
GTGGTAT17800.024.0656811
TGGTATC16400.024.0511532
TAAGGTG15800.022.857535
GTACATG63700.022.7895641
GTAAGGT15050.022.7351424
TACATGG61950.022.6298852
GCAGAGT100000.021.9477379
AGGTAAG14100.021.9076562
ACATGGG62850.021.7009263
GTATAGG5550.021.4398961
GAGTACT60500.021.15915312-13
Produced by FastQC (version 0.11.5)