FastQCFastQC Report
Thu 26 May 2016
SRR938466_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938466_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259215
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47610.2107369152559628No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42510.18816270253163156No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA31430.13911911880896682No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA30260.13394032883103202No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28630.12672543339168693No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28540.12632706493184578No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA27250.12061711700745613No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC26850.11884659051927329No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG24920.11030380021379108No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33200.057.4343721
GTATCAA58700.039.6938821
ATCAACG74750.030.5855773
TATCAAC76250.030.3076572
TCAACGC75800.030.1618984
CAACGCA78450.029.203635
AACGCAG81950.028.0512526
GTAAGGT14250.025.0166514
CGCAGAG91700.024.9133198
TGGTATC14700.024.9074542
TAAGGTG15700.024.8254435
ACGCAGA92700.024.644577
TATACCG1401.7713166E-523.7658185
GTGGTAT16850.023.1408621
AGGTAAG14700.022.3196662
TACATGG60850.021.33332
AAGGTAA15700.021.2013741
GGTAAGG17150.020.7864323
GCAGAGT107850.020.6982469
CAGAGTA98600.020.6650410-11
Produced by FastQC (version 0.11.5)